ALEXIS Biochemicals: Monoclonal Antibody to Fas (human) (SM1/1)

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Fas

Fas cDNA Probes

Fas Antibodies

Fas Kits & Sets

ALX-748-015

Revised 24-Mar-05

Fas (human) cDNA Probe
SYNONYMS	CD95 (human) cDNA Probe APO-1 (human) cDNA Probe TNFRSF 6 (human) cDNA Probe
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Fas cDNA Probes

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-748-015-C001		1 µg	140.00 USD

Product Specification

SOURCE/HOST:	Derived from the coding region of the human Fas (CD95) gene. Has a length of ~500bp located within the region between nucleotides 500-1000.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (agarose gel electrophoresis)
FORMULATION:	Lyophilized powder.
RECONSTITUTION:	Reconstitute with 10µl sterile water or 10mM Tris . HCl containing 0.1mM Na₂EDTA.
APPLICATION:	Northern Blot
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C

General Information

MANUFACTURER

Manufactured by Apotech Corporation.

Further Categories Containing This Product:

DNA & RNA Probes / Related Products

ALX-522-002

Revised 06-Jun-07

Fas (human):Fc (human) (recombinant)
SYNONYMS	APO-1 (human):Fc (human) (recombinant) CD95 (human):Fc (human) (recombinant) TNFRSF 6 (human):Fc (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Fas

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-002-C050		50 µg	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human Fas (CD95; APO-1) (aa 7-154) is fused to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions can be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds human, mouse and rat FasL (APO-1L; CD95L; CD178).
BIOLOGICAL ACTIVITY:	Inhibits the activity of human and mouse soluble Fas (sFasL) and thereby sFasL-mediated lysis of Fas sensitive cells in a concentration range of 20-100µg/ml. When used with cross-linking enhancer (see Set Prod. No. ALX-850-016), the inhibitory activity of rhFas:Fc is increased approximately 20- to 50-fold. Concentrations of rhFas:Fc required to inhibit may vary depending on the cell viability and on the concentrations of sFasL used to kill the cells.
APPLICATION:	ELISA (capture, 1µg/ml).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Images

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Product Specific Literature References

Characterization of Fas (Apo-1, CD95)-Fas ligand interaction: P. Schneider, et al.; J. Biol. Chem. 272, 18827 (1997) Abstract; Full Text
Cysteine 230 is essential for the structure and activity of the cytotoxic ligand TRAIL: J.L. Bodmer, et al.; J. Biol. Chem. 275, 20632 (2000) Abstract; Full Text
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000) Abstract
TGF-beta induces the expression of the FLICE-inhibitory protein and inhibits Fas-mediated apoptosis of microglia: R. Schlapbach, et al.; Eur. J. Immunol. 30, 3680 (2000) Abstract
Mechanisms underlying neuronal death induced by chromogranin A-activated microglia: J. Ciesielski-Treska, et al.; J. Biol. Chem. 276, 13113 (2001) Abstract; Full Text
T cell costimulation by the TNF ligand BAFF: B. Huard et al.; J. Immunol. 167, 6225 (2001) Abstract
Productive HIV-1 infection of primary CD4+ T cells induces mitochondrial membrane permeabilization leading to a caspase-independent cell death: F. Petit et al; J. Biol. Chem. 277, 1477 (2002) Abstract; Full Text
Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003) Abstract; Full Text
High expression of Fas ligand by synovial fluid-derived gamma delta T cells in Lyme arthritis: K. Roessner, et al.; J. Immunol. 170, 2702 (2003) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P25445: Fas (human) (precursor) AfCS Signalling Gateway link A000600: Fas (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

Related Products

ALX-203-004	Control:Fc Fusion Protein (human) (recombinant)
ALX-203-001	Enhancer for Receptors

Further Categories Containing This Product:

Receptor / Ligand + Enhancer Set • CD Kits & Sets

ALX-804-119

Revised 03-Nov-05

Monoclonal Antibody to Fas (DD) (human) (3D5)
SYNONYMS	anti-APO-1 (DD) (human) (3D5) anti-CD95 (DD) (human) (3D5) anti-TNFRSF 6 (DD) (human) MAb (3D5)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Fas Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-119-R200		200 µl	290.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	3D5
ISOTYPE:	Mouse IgG1
FORMULATION:	Liquid. In 50% glycerol containing 1mM sodium azide.
IMMUNOGEN:	Recombinant human Fas (CD95; APO-1) cytoplasmic domain.
SPECIFICITY:	Recognizes human Fas death domain (DD).
APPLICATION:	Western Blot (1:500; total cell lysates)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Activation-induced aggregation and processing of the human Fas antigen. Detection with cytoplasmic domain-specific antibodies: T. Kamitani, et al.; J. Biol. Chem. 272, 22307 (1997) Abstract; Full Text
Death of smooth muscle cells and expression of mediators of apoptosis by T lymphocytes in human abdominal aortic aneurysms: E.L. Henderson, et al.; Circulation 99, 96 (1999) Abstract; Full Text
Fas aggregation does not correlate with Fas-mediated apoptosis: Y. Lee & E. Shacter; J. Immunol. 167, 82 (2001) Abstract

Further Categories Containing This Product:

Monoclonal Antibodies • CD Antibodies

ALX-805-038

Revised 23-Jul-08

Monoclonal Antibody to Fas (human) (2R2)
SYNONYMS	anti-CD95 (human) MAb (2R2) anti-APO-1 (human) MAb (2R2) anti-TNFRSF 6 MAb (human) (2R2)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Fas Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-805-038-C100		100 µg	340.00 USD
ALX-805-038B-C100	Biotin	100 µg	390.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	2R2
ISOTYPE:	Mouse IgG3
SOURCE/HOST:	Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE).
PURITY DETAIL:	Protein A-affinity purified.
FORMULATION:	2R2: Liquid. In PBS containing 1% BSA. Contains no preservatives. 2R2-Biotin: Liquid. In PBS.
IMMUNOGEN:	Mouse L cells stably overexpressing human Fas (CD95; APO-1).
SPECIFICITY:	Recognizes human Fas.
APPLICATION:	2R2: Flow Cytometry Functional Application (induces apoptosis - no cross-linking required). - Can be used in DISC (death-inducing signalling complex assays): Co-immunoprecipitates FADD and caspase-8 after receptor activation. 2R2-Biotin: Flow Cytometry Immunocytochemistry Immunohistochemistry (frozen sections, paraffin sections) - Recommended working dilutions are 1:200-1:1000.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.

Product Images

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Product Specific Literature References

Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis: D. Ferrari, et al.; J. Exp. Med. 188, 979 (1998) Abstract; Full Text
Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction: S. Wesselborg, et al.; Blood 93, 3053 (1999) Abstract; Full Text
IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt, et al.; Eur. J. Immunol. 30, 1769 (2000) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

IHC (PS) Antigen Retrieval: we recommend using a citric acid solution together with microwave retrieval (5 x 5 minutes at high intensity).

Further Categories Containing This Product:

Monoclonal Antibodies • CD Antibodies

ALX-805-022

Revised 29-Jul-08

Monoclonal Antibody to Fas (human) (APO-1-1)
SYNONYMS	anti-CD95 (human) MAb (APO-1-1) anti-APO-1 (human) MAb (APO-1-1) anti-TNFRSF 6 (human) MAb (APO-1-1)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Fas Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-805-022-C100	Purified	100 µg	259.00 USD
ALX-805-022B-T100	Biotin	100 tests	308.00 USD
ALX-805-022F-T100	FITC	100 tests	308.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	APO-1-1
ISOTYPE:	Mouse IgG1
PURITY:	≥95% (SDS-PAGE).
FORMULATION:	APO-1-1-Purified: Liquid. 0.1mg/ml in PBS. APO-1-1-Biotin and -FITC: Liquid. In 1ml 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
IMMUNOGEN:	Human Fas (CD95; APO-1).
SPECIFICITY:	Recognizes human Fas.
APPLICATION:	Immunohistochemistry (frozen sections): APO-1-1 (ALX-805-022; IgG1) is an isotype-switch variant of the original clone APO-1-3 (ALX-805-020). Since undesired side reactions in immunohistochemistry are minimal for IgG1 antibodies, APO-1-1 is especially useful for immunohistology on cryostat sections and on cytospin preparations [1]. Note: Not suitable for stainings on paraffin sections. Flow Cytometry: APO-1-1-Purified: 1-2µg/ml APO-1-1-Biotin and -FITC: 1:20 Functional Application: Induces apoptosis upon cross-linking [2]. Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. After opening aliquot contents and store at -20°C.

Product Specific Literature References

[1] Constitutive and induced expression of APO-1, a new member of the nerve growth factor/tumor necrosis factor receptor superfamily, in normal and neoplastic cells: F. Leithauser, et al.; Lab. Invest. 69, 415 (1993) Abstract
[2] Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-x(L): D.C. Huang, et al.; PNAS 96, 14871 (1999) Abstract
[3] Inhibition of death receptor-mediated gene induction by a cycloheximide-sensitive factor occurs at the level of or upstream of Fas-associated death domain protein (FADD): H. Wajant, et al.; J. Biol. Chem. 275, 24357 (2000) Abstract; Full Text

Further Categories Containing This Product:

Monoclonal Antibodies • CD Antibodies

ALX-805-020

Revised 25-Apr-07

Monoclonal Antibody to Fas (human) (APO-1-3)
SYNONYMS	anti-CD95 (human) MAb (APO-1-3) anti-APO-1 (human) MAb (APO-1-3) anti-TNFRSF 6 (human) MAb (APO-1-3)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Fas Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-805-020-C100	Purified	100 µg	295.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	APO-1-3
ISOTYPE:	Mouse IgG3
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. 1mg/ml in PBS. Contains no preservatives.
IMMUNOGEN:	Human recombinant Fas (CD95; APO-1).
SPECIFICITY:	Recognizes human Fas.
APPLICATION:	Flow Cytometry (suitable as a primary antibody) Immunoprecipitation Western Blot Functional Application: Induces apoptosis with or without cross-linking (Protein A), depending on cell type.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Specific Literature References

Monoclonal antibody-mediated tumor regression by induction of apoptosis: B.C. Trauth, et al.; Science 245, 301 (1989) Abstract
Monoclonal-antibody-mediated apoptosis in adult T-cell leukaemia: K.M. Debatin, et al.; Lancet 335, 497 (1990) Abstract
Induction of apoptosis by monoclonal antibody anti-APO-1 class switch variants is dependent on cross-linking of APO-1 cell surface antigens: J. Dhein, et al.; J. Immunol. 149, 3166 (1992) Abstract
APO-1-induced apoptosis of leukemia cells from patients with adult T-cell leukemia: K.M. Debatin, et al.; Blood 81, 2972 (1993) Abstract
Apoptotic cell death induced by a mouse-human anti-APO-1 chimeric antibody leads to tumor regression: L.R. Coney, et al.; Int. J. Cancer 58, 562 (1994) Abstract
Activation induces sensitivity toward APO-1 (CD95)-mediated apoptosis in human B cells: P.T. Daniel & P.H. Krammer; J. Immunol. 152, 5624 (1994) Abstract
APO-1 (CD95) mediated apoptosis in human T-ALL engrafted in SCID mice: K.M. Lücking-Famira, et al.; Leukemia 8, 1825 (1994) Abstract
The apoptosis-1/Fas protein in human systemic lupus erythematosus: E. Mysler, et al.; J. Clin. Invest. 93, 1029 (1994) Abstract
Divergent signalling via APO-1/Fas and the TNF receptor, two homologous molecules involved in physiological cell death: K. Schulze-Osthoff, et al.; EMBO J. 13, 4587 (1994) Abstract
Cell nucleus and DNA fragmentation are not required for apoptosis: K. Schulze-Osthoff, et al.; J. Cell Biol. 127, 15 (1994) Abstract
Inhibition of apoptosis in T cells expressing human T cell leukemia virus type I Tax: K.F. Copeland, et al.; AIDS Res. Hum. Retroviruses 10, 1259 (1994) Abstract
Resistance to APO-1 (CD95) induced apoptosis in T-ALL is determined by a BCL-2 independent anti-apoptotic program: K.M. Debatin & P.H. Krammer; Leukemia 9, 815 (1995) Abstract
Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor: F.C. Kischkel, et al.; EMBO J. 14, 5579 (1995) Abstract
CD40 ligation induces Apo-1/Fas expression on human B lymphocytes and facilitates apoptosis through the Apo-1/Fas pathway: E.J. Schattner, et al.; J. Exp. Med. 182, 1557 (1995) Abstract
Sensitization of T cells to CD95-mediated apoptosis by HIV-1 Tat and gp120: M.O. Westendorp, et al.; Nature 375, 497 (1995) Abstract
Autocrine T-cell suicide mediated by APO-1/(Fas/CD95): J. Dhein, et al.; Nature 373, 438 (1995) Abstract
The medical significance of physiological cell death: G. Häcker & D.L. Vaux; Med. Res. Rev. 15, 299 (1995) Abstract
Involvement of the CD95 (APO-1/FAS) receptor/ligand system in drug-induced apoptosis in leukemia cells: C. Friesen, et al.; Nat. Med. 2, 574 (1996) Abstract
Apoptosis and the maintenance of homoeostasis in the immune system: B.A. Osborne; Curr. Opin. Immunol. 8, 245 (1996) Abstract
Latent sensitivity to Fas-mediated apoptosis after CD40 ligation may explain activity of CD154 gene therapy in chronic lymphocytic leukemia: P. Chu, et al.; PNAS 99, 3854 (2002) Abstract; Full Text

Further Categories Containing This Product:

Monoclonal Antibodies • CD Antibodies

ALX-805-010

Revised 29-Apr-08

Monoclonal Antibody to Fas (human) (blocking) (SM1/23)
SYNONYMS	anti-CD95 (human) MAb (blocking) (SM1/23) anti-APO-1 (human) MAb (blocking) (SM1/23) anti-TNFRSF 6 (human) MAb (blocking) (SM1/23)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Fas Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-805-010-C100	Purified	100 µg	259.00 USD
ALX-805-010B-T100	Biotin	100 tests	308.00 USD
ALX-805-010F-T100	FITC	100 tests	308.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	SM1/23
ISOTYPE:	Mouse IgG2b
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	SM1/23: 1mg/ml in PBS. SM1/23-Biotin and -FITC: Liquid. In 1ml 50mM TRIS, pH 7.4, containing 100mM NaCl, 1% BSA and 0.02% sodium azide.
IMMUNOGEN:	Human Fas (CD95; APO-1).
SPECIFICITY:	Recognizes human Fas.
APPLICATION:	Flow Cytometry Functional Application (blocks induction of apoptosis induced by clone SM1/1 (Prod. No. ALX-805-008) or other commercial anti-Fas antibodies). Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Further Categories Containing This Product:

Monoclonal Antibodies • CD Antibodies

ALX-805-008

Revised 13-Jun-06

Monoclonal Antibody to Fas (human) (SM1/1)
SYNONYMS	anti-CD95 (human) MAb (SM1/1) anti-APO-1 (human) MAb (SM1/1) anti-TNFRSF 6 (human) MAb (SM1/1)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Fas Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-805-008-C100		100 µg	259.00 USD

BACKGROUND/TECHNICAL INFORMATION	Enhancer for sFasL and rhFas:Fc are not identical and may show decreased efficiency when used together. We recommend to only use enhancer for rhFas:Fc. Swiss-Prot link P25445: Fas (human) (precursor) AfCS Signalling Gateway link A000600: Fas (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.