ALEXIS Biochemicals: Polyclonal Antibody to β-Pix

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ALX-480-093

Revised 07-Feb-08

8-(4-Chlorophenylthio)-2'-O-methyladenosine 3',5'-cyclic Monophosphate . sodium salt
SYNONYMS	8-CPT-2'-O-Me-cAMP . Na
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	cAMP Derivatives

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-480-093-M001		1 mg	130.00 USD
ALX-480-093-M005		5 mg	520.00 USD

Product Specification

FORMULA:	C₁₇H₁₆ClN₅O₆PS . Na
MW:	484.8 . 23.0
CAS NUMBER:	510774-50-2
PURITY:	≥98% (HPLC)
PURITY DETAIL:	Product is not sterile.
FORMULATION:	White to off-white powder.
SOLUBILITY:	Soluble in water, aqueous buffers, DMSO, dimethyl formamide or methanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. Hygroscopic.
IDENTITY:	Determined by ¹H-NMR, MS and UV (λ_max: 282nm, ε: 16’000, pH 7).

Product Description

Specific, potent and membrane permeant activator of the Epac cAMP receptor. Activates Epac1 and Epac2, the guanine nucleotide-exchange factors (GEFs) for the small GTPases Rap1 and Rap2. Does not activate protein kinase A (PKA), neither in vitro nor in vivo and is therefore a valuable tool to specifically discriminate between PKA- and Epac-mediated effects. Since a free 2'-ribose hydroxyl group in cyclic AMP is essential for stimulation of PKA, the methylated structure of 8-CPT-2'-O-Me-cAMP is an extremely poor PKA activator. Its high lipophilicity allows for good membrane permeability in most biosystems and its increased resistance towards phosphodiesterases prevents rapid hydrolysis.

Product Specific Literature References

A novel Epac-specific cAMP analogue demonstrates independent regulation of Rap1 and ERK: J.M. Enserink, et al.; Nat. Cell Biol. 4, 901 (2002) Abstract; Full Text
Epac-selective cAMP analog 8-pCPT-2'-O-Me-cAMP as a stimulus for Ca2+-induced Ca2+ release and exocytosis in pancreatic beta-cells: G. Kang, et al.; J. Biol. Chem. 278, 8279 (2003) Abstract; Full Text
Cyclic AMP induces integrin-mediated cell adhesion through Epac and Rap1 upon stimulation of the {beta}2-adrenergic receptor: S. Rangarajan, et al.; J. Cell Biol. 160, 487 (2003) Abstract

General Literature References

Signal transduction. New exchange, new target: J. Downward; Nature 396, 416 (1998), Comment Abstract
Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP: J. de Rooij, et al.; Nature 396, 474 (1998) Abstract
A family of cAMP-binding proteins that directly activate Rap1: H. Kawasaki, et al.; Science 282, 2275 (1998) Abstract

Further Categories Containing This Product:

PKA Activators • Guanine-nucleotide Exchange Factors [GEFs]

ALX-210-380

Revised 30-Oct-06

Polyclonal Antibody to β-Pix
SYNONYMS	anti-p85SPR PAb anti-PAK-interacting Exchange Factor β PAb anti-Rho Guanine Nucleotide Exchange Factor 7 PAb anti-COOL-1 PAb
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Guanine-nucleotide Exchange Factors [GEFs]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-380-C100		100 µg	290.00 USD
ALX-210-380B-C050	Biotin	50 µg	290.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml.
FORMULATION:	Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:	Recombinant β-Pix (PAK-interacting exchange factor β) SH3 domain.
SPECIFICITY:	Recognizes the multiple splice variants of human, mouse and rat β-Pix-a, -b, bL, -c and -d isoforms.
APPLICATION:	ELISA (direct and indirect: 1:5'000-1:10'000) Immunocytochemistry (1:1'000) Western Blot (1:5'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

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Product Specific Literature References

Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion: W.K. Oh, et al.; BBRC 235, 794 (1997) Abstract
betaPix-b(L), a novel isoform of betaPix, is generated by alternative translation: S. Rhee, et al.; BBRC 318, 415 (2004) Abstract

General Information

β-Pix is a p21-activated protein kinase (Pak)-interacting exchange factor that has been identified as a putative guanine nucleotide exchange factor (GEF) for Rac/Cdc42. β-Pix is expressed ubiquitously in all the tissue examined and other isoforms are expressed mainly in the central nervous system.
MANUFACTURER	Manufactured by AdipoGen, Inc.

General Literature References

A novel regulator of p21-activated kinases: S. Bagrodia, et al.; J. Biol. Chem. 273, 23633 (1998) Abstract
Leucine zipper-mediated homodimerization of the p21-activated kinase-interacting factor, beta Pix. Implication for a role in cytoskeletal reorganization: S. Kim, et al.; J. Biol. Chem. 276, 10581 (2001) Abstract
Human immunodeficiency virus type 1 Nef selectively associates with a catalytically active subpopulation of p21-activated kinase 2 (PAK2) independently of PAK2 binding to Nck or beta-PIX: G.H. Renkema, et al.; J. Virol. 75, 2154 (2001) Abstract
Genomic structure of the human beta-PIX gene and its alteration in gastric cancer: Z. Li, et al.; Cancer Lett. 177, 203 (2002) Abstract
Phosphorylation of p85 beta PIX, a Rac/Cdc42-specific guanine nucleotide exchange factor, via the Ras/ERK/PAK2 pathway is required for basic fibroblast growth factor-induced neurite outgrowth: E.Y. Shin, et al.; J. Biol. Chem. 277, 44417 (2002) Abstract
Mammalian Scribble forms a tight complex with the betaPIX exchange factor: S. Audebert, et al.; Curr. Biol. 14, 987 (2004) Abstract

Further Categories Containing This Product:

Polyclonal Antibodies

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