© 2008 Alexis Corporation
14-3-3 Proteins / Related Products
You are here: Product Lines > Signal Transduction > Adaptor Proteins & Interaction Domains / Related Products > 14-3-3 Proteins / Related Products
ALX-350-014 Revised 03-Apr-08
Calyculin A
Add to Clipboard
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Other Signal Transduction Pathway Modulators
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-014-C010   10 µg 65.00 USD Add To Cart
ALX-350-014-C025   25 µg 110.00 USD Add To Cart
ALX-350-014-C050   50 µg 190.00 USD Add To Cart
ALX-350-014-C100   100 µg 320.00 USD Add To Cart
Product Specification
FORMULA: C50H81N4O15P
MW: 1009.2
CAS NUMBER: 101932-71-2
SOURCE/HOST: Isolated from Discodermia calyx.
PURITY: ≥95%
APPEARANCE: Lyophilized.
SOLUBILITY: Soluble in DMSO, 100% ethanol, methanol, acetonitrile, chloroform, methylene chloride or benzene. Insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: May by subject to isomerization when in solution. Biological activity is not affected by isomerization.
HANDLING: Protect from light and moisture.
HAZARD: TOXIC. MAY BE CARCINOGENIC.

Product Description
Potent cell permeable inhibitor with high specificity for protein phosphatase 1 (PP1) and 2A (PP2A). Inhibits p130cas tyrosine phosphorylation. Enhances the phosphorylation level of NF-κB. Affects intracellular signalling processes that require 14-3-3. Potent non-phorbol type tumor promoter. Prevents γ-radiation induced apoptosis.
Product Specific Literature References
Okadaic acid: an additional non-phorbol-12-tetradecanoate-13-acetate- type tumor promoter: M. Suganuma, et al.; PNAS 85, 1768 (1988) Abstract
Protein phosphatases come of age: P.Cohen & P.T. Cohen; J. Biol. Chem. 264, 21435 (1989) Abstract; Full Text
Calyculin A and okadaic acid: inhibitors of protein phosphatase activity: H. Ishihara, et al.; BBRC 159, 871 (1989) Abstract
The structure and regulation of protein phosphatases: P. Cohen; Ann. Rev. Biochem. 58, 453 (1989) Abstract
Calyculin A, an inhibitor of protein phosphatases, a potent tumor promoter on CD-1 mouse skin: M. Suganuma, et al.; Cancer Res. 50, 3521 (1990) Abstract
Nonphorbol tumor promoters okadaic acid and calyculin-A induce membrane translocation of protein kinase C: R. Gopalakrishna; BBRC 189, 950 (1992) Abstract
Comparative effects of protein phosphatase inhibitors (okadaic acid and calyculin A) on human leukemia HL60, HL60/ADR and K562 cells: K. Sakurada, et al.; BBRC 187, 488 (1992) Abstract
Calyculin A, a potent inhibitor of phosphatases-1 and -2A, prevents apoptosis: Q. Song & M.F. Lavin; BBRC 190, 47 (1993) Abstract
Enhancement of AMPA-mediated synaptic transmission by the protein phosphatase inhibitor calyculin A in rat hippocampal slices: A. Figurov, et al.; Eur. J. Neurosci. 5, 1035 (1993) Abstract
Calyculin A, a non-phorbol ester type tumor promotor, induced oxidative DNA damage in stimulated human neutrophil-like cells: T. Takeuchi, et al.; BBRC 205, 1803 (1994) Abstract
Potassium and calcium channel involvement in induction of long-lasting synaptic enhancement by calyculin A, a protein phosphatase inhibitor, in rat hippocampal CA1 region: N. Murakami, et al.; Neurosci. Lett. 176, 181 (1994) Abstract
Differential toxicity of the protein phosphatase inhibitors microcystin and calyculin A: M.T. Runnegar, et al.; J. Pharmacol. Exp. Ther. 273, 545 (1995) Abstract
Inhibition of p130cas tyrosine phosphorylation by calyculin A: W. Qiu, et al.; J. Leukoc. Biol. 63, 631 (1998) Abstract
Unique features of the okadaic acid activity class of tumor promoters: H. Fujiki & M. Suganuma; J. Cancer Res. Clin. Oncol. 125, 150 (1999), Review Abstract
Calyculin A-induced vimentin phosphorylation sequesters 14-3-3 and displaces other 14-3-3 partners in vivo: G. Tzivion, et al.; J. Biol. Chem. 275, 29772 (2000) Abstract
Calyculin A induces apoptosis and stimulates phosphorylation of p65NF-kappaB in human osteoblastic osteosarcoma MG63 cells: H. Tanaka, et al.; Int. J. Oncol. 31, 389 (2007) Abstract
Further Categories Containing This Product:
PP2A / Related Products • PP1 / Related Products • Natural Products - Other Tumor Promoters • 14-3-3 Proteins / Related Products • Carcinogens & Tumor Promoters Other Products • NF-kB Pathway Modulators • Natural Products - Apoptosis Inducers & Inhibitors • Marine Natural Products
 
 
ALX-350-115 Revised 21-Feb-08
Fusicoccin
Add to Clipboard
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Plant Research Reagents
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-115-MC05   0.5 mg 70.00 USD Add To Cart
ALX-350-115-M001   1 mg 120.00 USD Add To Cart
Product Specification
FORMULA: C36H56O12
MW: 680.8
CAS NUMBER: 20108-30-9
SOURCE/HOST: Isolated from fungus Fusicoccum amygdali.
PURITY: ≥90% (HPLC)
APPEARANCE: White to off-white crystalline solid.
SOLUBILITY: Soluble in acetone, 100% ethanol, methanol, chloroform or ethyl acetate; practically insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: TOXIC.

Product Description
Diterpene glucoside with numerous effects on plants. Induces H+ extrusion from plant cells by 14-3-3-dependent activation of the plasma membrane H+-ATPase. Stimulates cell enlargement, ion uptake, seed germination, opening the stomata, etc. Markedly reduces the cell’s capability for H2O2 scavenging.
Product Specific Literature References
Fusicoccin - a key to multiple 14-3-3 locks?: A.H. De Boer; Trends Plant Sci. 2, 20 (1997)
Fusicoccin binding to its plasma membrane receptor and the activation of the plasma membrane H(+)-ATPase. IV. Fusicoccin induces the association between the plasma membrane H(+)-ATPase and the fusicoccin receptor: C. Olivari, et al.; Plant Physiol. 116, 529 (1998) Abstract
Cytoskeletal inhibitors suppress the stomatal opening of Vicia faba L. induced by fusicoccin and IAA: R.F. Huang, et al.; Plant Sci. 156, 65 (2000) Abstract
Inhibition of catalase activity as an early response of Arabidopsis thaliana cultured cells to the phytotoxin fusicoccin: N. Beffagna & I. Lutzu; J. Exp. Bot. 58, 4183 (2007) Abstract
General Literature References
Fusicoccin: a tool in plant physiology: E. Marre; Ann. Rev. Plant Physiol. 30, 273 (1979), (Review)
Fusicoccin receptors: perception and transduction of the fusicoccin signal: P. Aducci et al.; J. Exp. Bot. 46, 1463 (1995), (Review)
Fusicoccin - a key to multiple 14-3-3 locks?: A.H. De Boer; Trends Plant Sci. 2, 20 (1997), (Review)
Fusicoccin binding to its plasma membrane receptor and the activation of the plasma membrane H(+)-ATPase. IV. Fusicoccin induces the association between the plasma membrane H(+)-ATPase and the fusicoccin receptor: C. Olivari, et al.; Plant Physiol. 116, 529 (1998) Abstract
Phosphorylation-independent interaction between 14-3-3 protein and the plant plasma membrane H+-ATPase: J. Borch, et al.; Biochem. Soc. Trans. 30, 411 (2002), (Review) Abstract; Full Text
Plant 14-3-3 proteins assist ion channels and pumps: A.H. de Boer; Biochem. Soc. Trans. 30, 416 (2002), (Review) Abstract; Full Text
Metabolic enzymes as targets for 14-3-3 proteins: S.C. Huber, et al.; Plant Mol. Biol. 50, 1053 (2002), (Review) Abstract
cDNA microarray analysis of fusicoccin-induced changes in gene expression in tomato plants: U.B. Frick & A. Schaller; Planta 216, 83 (2002) Abstract
Mutational analysis of the interaction between 14-3-3 proteins and plant plasma membrane H+-ATPase: S. Visconti, et al.; J. Biol. Chem. 278, 8172 (2003) Abstract
Structural view of a fungal toxin acting on a 14-3-3 regulatory complex: M. Wurtele, et al.; Embo J. 22, 987 (2003) Abstract
Further Categories Containing This Product:
H+-ATPase / Related Products • 14-3-3 Proteins / Related Products • Plant Research Reagents / Related Products
 
 
ALX-215-025 Revised 21-Jul-06
Polyclonal Antibody to 14-3-3ε (NT)
Add to Clipboard
SYNONYMS anti-KCIP-1 PAb (NT)
anti-Protein Kinase C Inhibitor Protein 1 PAb (NT)
anti-14-3-3E PAb (NT)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-215-025-R100   100 µl 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum. Contains no preservatives.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-12 (M1DDREDLVYQAK12C) of 14-3-3ε.
SPECIFICITY: Recognizes human, mouse, rat, sheep, bovine and chicken 14-3-3ε.
APPLICATION: ELISA
Immunohistochemistry (1:400)
Western Blot (1:3000)
Optimal condition must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein: H. Martin, et al.; FEBS Lett. 331, 296 (1993) Abstract; Full Text
Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies: H. Martin, et al.; J. Neurochem. 63, 2259 (1994) Abstract
Purification of 14-3-3 protein and analysis of isoforms in chicken brain: Y. Patel, et al.; Biochim. Biophys. Acta 1222, 405 (1994) Abstract
Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins: D.H. Jones, et al.; FEBS Lett. 368, 55 (1995) Abstract; Full Text
Expression and structural analysis of 14-3-3 proteins: D.H. Jones, et al.; J. Mol. Biol. 245, 375 (1995) Abstract
Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation: C.P. Wheeler-Jones, et al.; Biochem. J. 315, 41 (1996) Abstract; Full Text
14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain: M. Matto-Yelin, et al.; Mol. Biol. Cell 8, 1889 (1997) Abstract; Full Text
Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease: J. Wiltfang, et al.; J. Neurochem. 73, 2485 (1999) Abstract
Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants: A. Aitken; Plant Mol. Biol. 50, 993 (2002) Abstract
Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain: H.C. Baxter, et al.; Neuroscience 109, 5 (2002) Abstract
Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1: H.K. Chen, et al.; Cell 113, 457 (2003) Abstract
Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein: P. Steinacker, et al.; Mol. Cell. Biol. 25, 1339 (2005) Abstract; Full Text
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-708 Revised 30-Jun-06
Polyclonal Antibody to 14-3-3 (human)
Add to Clipboard
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-708-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.1mg/ml
PURITY DETAIL: Protein A-affinity purified IgG.
FORMULATION: Liquid. In 0.05M sodium phosphate buffer containing 0.1% sodium azide and 0.2% gelatin.
IMMUNOGEN: Synthetic peptide corresponding to aa 221-242 of 14-3-3 protein family members.
SPECIFICITY: Recognizes human 14-3-3 protein family members.
APPLICATION: Western Blot (2.5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Do not freeze.
General Information
General Literature References
[1] 14-3-3 proteins. Hot numbers in signal transduction: P.D. Burbelo and A. Hall; Curr. Biol. 5, 95 (1995) Abstract
[2] Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins: D.H. Jones, et al.; FEBS Lett. 368, 55 (1995) Abstract
[3] 14-3-3 proteins: a highly conserved, widespread family of eukaryotic proteins: A. Aitken, et al.; TIBS 17, 498 (1992), (Review) Abstract
[4] Evolutionary conservation of the 14-3-3 protein: G.J. Martens, et al.; BBRC 184, 1456 (1992) Abstract
[5] Expression and structural analysis of 14-3-3 proteins: D.H. Jones, et al.; D.H. Jones, et al. 245, 375 (1995) Abstract
[6] Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies : H. Martin, et al.; J. Neurochem. 63, 2259 (1994) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-215-028 Revised 17-Aug-06
Polyclonal Antibody to 14-3-3τ
Add to Clipboard
SYNONYMS anti-14-3-3θ PAb
anti-KCIP-1 PAb
anti-Protein Kinase C Inhibitor Protein 1 PAb
anti-HS1 Protein PAb
anti-T Cell Protein PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-215-028-R100   100 µl 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum. Contains no preservatives.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-10 (M1EKTELIQKA10C) of 14-3-3τ.
SPECIFICITY: Recognizes human, mouse, rat, sheep, bovine and chicken 14-3-3τ.
APPLICATION: ELISA
Immunohistochemistry (1:60)
Western Blot (1:3000)
Optimal condition must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein: H. Martin, et al.; FEBS Lett. 331, 296 (1993) Abstract; Full Text
Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies: H. Martin, et al.; J. Neurochem. 63, 2259 (1994) Abstract
Purification of 14-3-3 protein and analysis of isoforms in chicken brain: Y. Patel, et al.; Biochim. Biophys. Acta 1222, 405 (1994) Abstract
Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins: D.H. Jones, et al.; FEBS Lett. 368, 55 (1995) Abstract; Full Text
Expression and structural analysis of 14-3-3 proteins: D.H. Jones, et al.; J. Mol. Biol. 245, 375 (1995) Abstract
Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation: C.P. Wheeler-Jones, et al.; Biochem. J. 315, 41 (1996) Abstract; Full Text
14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain: M. Matto-Yelin, et al.; Mol. Biol. Cell 8, 1889 (1997) Abstract; Full Text
Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease: J. Wiltfang, et al.; J. Neurochem. 73, 2485 (1999) Abstract
Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants: A. Aitken; Plant Mol. Biol. 50, 993 (2002) Abstract
Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain: H.C. Baxter, et al.; Neuroscience 109, 5 (2002) Abstract
Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1: H.K. Chen, et al.; Cell 113, 457 (2003) Abstract
Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein: P. Steinacker, et al.; Mol. Cell. Biol. 25, 1339 (2005) Abstract; Full Text
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-215-029 Revised 21-Jul-06
Polyclonal Antibody to 14-3-3ζ
Add to Clipboard
SYNONYMS anti-KCIP-1 PAb
anti-Protein Kinase C Inhibitor Protein 1 PAb
anti-14-3-3δ PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-215-029-R100   100 µl 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum. Contains no preservatives.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-10 (M1DKNELVQKA10C) of 14-3-3ζ.
SPECIFICITY: Recognizes human, mouse, rat, sheep, bovine and chicken 14-3-3ζ.
APPLICATION: ELISA
Immunohistochemistry (1:400)
Western Blot (1:3000)
Optimal condition must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein: H. Martin, et al.; FEBS Lett. 331, 296 (1993) Abstract; Full Text
Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies: H. Martin, et al.; J. Neurochem. 63, 2259 (1994) Abstract
Purification of 14-3-3 protein and analysis of isoforms in chicken brain: Y. Patel, et al.; Biochim. Biophys. Acta 1222, 405 (1994) Abstract
Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins: D.H. Jones, et al.; FEBS Lett. 368, 55 (1995) Abstract; Full Text
Expression and structural analysis of 14-3-3 proteins: D.H. Jones, et al.; J. Mol. Biol. 245, 375 (1995) Abstract
Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation: C.P. Wheeler-Jones, et al.; Biochem. J. 315, 41 (1996) Abstract; Full Text
14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain: M. Matto-Yelin, et al.; Mol. Biol. Cell 8, 1889 (1997) Abstract; Full Text
Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease: J. Wiltfang, et al.; J. Neurochem. 73, 2485 (1999) Abstract
Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants: A. Aitken; Plant Mol. Biol. 50, 993 (2002) Abstract
Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain: H.C. Baxter, et al.; Neuroscience 109, 5 (2002) Abstract
Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1: H.K. Chen, et al.; Cell 113, 457 (2003) Abstract
Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein: P. Steinacker, et al.; Mol. Cell. Biol. 25, 1339 (2005) Abstract; Full Text
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-215-030 Revised 12-Feb-07
Polyclonal Antibody to 14-3-3
Add to Clipboard
SYNONYMS anti-pan-14-3-3 PAb
anti-KCIP-1 PAb
anti-Protein Kinase C Inhibitor Protein 1 PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-215-030-R100   100 µl 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum. Contains no preservatives.
IMMUNOGEN: Mixture of 14-3-3 isoforms isolated from sheep brain.
SPECIFICITY: Recognizes human, mouse, rat, sheep, bovine and chicken 14-3-3. Detects a band of ~28kDa by Western blot.
APPLICATION: Immunohistochemistry
Immunoprecipitation
Western Blot (1:2000)
Optimal condition must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Mammalian and yeast 14-3-3 isoforms form distinct patterns of dimers in vivo: M. Chaudhri, et al.; BBRC 300, 679 (2003) Abstract
14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain: M. Matto-Yelin, et al.; Mol. Biol. Cell 8, 1889 (1997) Abstract; Full Text
Neurofibrillary tangles of Alzheimer’s disease brains contain 14-3-3 proteins: R. Layfield, et al.; Neurosci. Lett. 209, 57 (1996) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-215-021 Revised 21-Jul-06
Polyclonal Antibody to 14-3-3β
Add to Clipboard
SYNONYMS anti-KCIP-1 PAb
anti-Protein Kinase C Inhibitor Protein 1 PAb
anti-Protein 1054 PAb
anti-14-3-3α PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-215-021-R100   100 µl 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum. Contains no preservatives.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-8 (T1MDKSELV8C) of 14-3-3β.
SPECIFICITY: Recognizes human, mouse, rat, sheep and bovine 14-3-3β.
APPLICATION: ELISA
Immunohistochemistry (1:600)
Western Blot (1:3000)
Optimal condition must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein: H. Martin, et al.; FEBS Lett. 331, 296 (1993) Abstract; Full Text
Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies: H. Martin, et al.; J. Neurochem. 63, 2259 (1994) Abstract
Purification of 14-3-3 protein and analysis of isoforms in chicken brain: Y. Patel, et al.; Biochim. Biophys. Acta 1222, 405 (1994) Abstract
Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins: D.H. Jones, et al.; FEBS Lett. 368, 55 (1995) Abstract; Full Text
Expression and structural analysis of 14-3-3 proteins: D.H. Jones, et al.; J. Mol. Biol. 245, 375 (1995) Abstract
Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation: C.P. Wheeler-Jones, et al.; Biochem. J. 315, 41 (1996) Abstract; Full Text
14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain: M. Matto-Yelin, et al.; Mol. Biol. Cell 8, 1889 (1997) Abstract; Full Text
Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease: J. Wiltfang, et al.; J. Neurochem. 73, 2485 (1999) Abstract
Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants: A. Aitken; Plant Mol. Biol. 50, 993 (2002) Abstract
Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain: H.C. Baxter, et al.; Neuroscience 109, 5 (2002) Abstract
Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1: H.K. Chen, et al.; Cell 113, 457 (2003) Abstract
Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein: P. Steinacker, et al.; Mol. Cell. Biol. 25, 1339 (2005) Abstract; Full Text
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-215-026 Revised 21-Jul-06
Polyclonal Antibody to 14-3-3β/ζ (phosphorylated) (pSer185)
Add to Clipboard
SYNONYMS anti-KCIP-1 (phosphorylated) (pSer185) PAb
anti-Protein Kinase C Inhibitor Protein 1 (phosphorylated) (pSer185) PAb
anti-Protein 1054 (phosphorylated) (pSer185) PAb
anti-14-3-3α/δ (phosphorylated) (pSer185) PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-215-026-R100   100 µl 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From sheep.
PURITY DETAIL: Affinity-purified.
FORMULATION: Liquid. Contains no preservatives.
IMMUNOGEN: Synthetic phosphorylated peptide corresponding to aa 181-190 (E181ILNpSPEKAC190) of 14-3-3β and aa 180-189 of 14-3-3ζ.
SPECIFICITY: Recognizes human, mouse, rat, sheep and bovine 14-3-3β and 14-3-3ζ.
APPLICATION: Western Blot (1:300)
Optimal condition must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Phospho-specific Antibodies • Polyclonal Antibodies
 
 
ALX-215-022 Revised 21-Jul-06
Polyclonal Antibody to 14-3-3ε (CT)
Add to Clipboard
SYNONYMS anti-KCIP-1 PAb (CT)
anti-Protein Kinase C Inhibitor Protein 1 PAb (CT)
anti-14-3-3E PAb (CT)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-215-022-R100   100 µl 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum. Contains no preservatives.
IMMUNOGEN: Synthetic peptide corresponding to aa 239-255 (CG239EEQNKEALQDVEDENQ255) of 14-3-3ε.
SPECIFICITY: Recognizes human, mouse, rat, sheep, bovine and chicken 14-3-3ε.
APPLICATION: ELISA
Immunohistochemistry
Western Blot (1:3000)
Optimal condition must be determined i