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ALX-350-332 Revised 20-Oct-08
Celastrol
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SYNONYMS Tripterine
3-Hydroxy-24-nor-2-oxo-1(10),3,5,7-friedelatetraen-29-oic acid
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Anti-inflammatory Agents
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-332-M005   5 mg 65.00 USD Add To Cart
ALX-350-332-M025   25 mg 260.00 USD Add To Cart
Product Specification
FORMULA: C29H38O4
MW: 450.6
CAS NUMBER: 34157-83-0
SOURCE/HOST: Isolated from the root of Tripterygium sp.
PURITY: ≥98% (HPLC)
APPEARANCE: Red cubic crystals.
SOLUBILITY: Soluble in 100% ethanol, DMSO or dimethyl formamide; insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Product Description
Cell permeable triterpenoid; anti-inflammatory and immunosuppressive compound. Antioxidant. Suppresses LPS-induced cytokine release in macrophages and monocytes (IC50=40nM for IL-1β and IL-1α, 80nM for IL-6, 110nM for prostaglandin E2 and 210nM for IL-8 and TNF-α). Suppresses nitric oxide (NO) production (IC50=230nM) and LPS-induced NF-κB activation (IC50=270nM). Inhibits chymotrypsin-like activity of 20S proteasome (IC50=2.5µM). Inhibits lipid peroxidation induced by ADP and Fe2+ in rat liver mitochondria (IC50=7µM). Induces expression of a wider set of heat shock proteins. Inhibits topoisomerse II.
Product Specific Literature References
The triterpene celastrol as a very potent inhibitor of lipid peroxidation in mitochondria: H. Sassa, et al.; BBRC 172, 890 (1990) Abstract
Structural basis of potent antiperoxidation activity of the triterpene celastrol in mitochondria: effect of negative membrane surface charge on lipid peroxidation: H. Sassa, et al.; Free Radic. Biol. Med. 17, 201 (1994) Abstract
Novel cytokine release inhibitors. Part III: Truncated analogs of tripterine: W. He, et al.; Bioorg. Med. Chem. Lett. 8, 3659 (1998) Abstract
Celastrol, a potent antioxidant and anti-inflammatory drug, as a possible treatment for Alzheimer’s disease: A.C. Allison, et al.; Prog. Neuropsychopharmacol. Biol. Psychiatry 25, 1341 (2001) Abstract
Antiinflammatory constituents of Celastrus orbiculatus inhibit the NF-kappaB activation and NO production: H.Z. Jin, et al.; J. Nat. Prod. 65, 89 (2002) Abstract
Apoptosis induction in HL-60 cells and inhibition of topoisomerase II by triterpene celastrol: M. Nagase, et al.; Biosci. Biotechnol. Biochem. 67, 1883 (2003) Abstract; Full Text
Celastrol inhibits pro-inflammatory cytokine secretion in Crohn’s disease biopsies: G.F. Pinna, et al.; BBRC 322, 778 (2004) Abstract
Celastrols as inducers of the heat shock response and cytoprotection: S.D. Westerheide, et al.; J. Biol. Chem. 279, 56053 (2004) Abstract; Full Text
Celastrol protects against MPTP- and 3-nitropropionic acid-induced neurotoxicity: C. Cleren, et al.; J. Neurochem. 94, 995 (2005) Abstract
Celastrol blocks neuronal cell death and extends life in transgenic mouse model of amyotrophic lateral sclerosis: M. Kiaei, et al.; Neurodegener. Dis. 2, 246 (2005) Abstract
Celastrol, a triterpene extracted from the Chinese "Thunder of God Vine," is a potent proteasome inhibitor and suppresses human prostate cancer growth in nude mice: H. Yang, et al.; Cancer Res. 66, 4758 (2006) Abstract; Full Text
Celastrol, a novel triterpene, potentiates TNF-induced apoptosis and suppresses invasion of tumor cells by inhibiting NF-kappaB-regulated gene products and TAK1-mediated NF-kappaB activation: G. Sethi, et al.; Blood 109, 2727 (2007) Abstract
Induction of heat shock proteins in differentiated human and rodent neurons by celastrol: A.M. Chow & I.R. Brown; Cell Stress Chaperones 12, 237 (2007) Abstract
Further Categories Containing This Product:
Stress & Heat Shock Proteins Other Products • NF-kB Pathway Inhibitors • Natural Products - Topoisomerase Inhibitors • Natural Products - Antioxidants • Ubiquitin & Proteasome Other Products • Natural Products - Nitric Oxide Pathway Modulators • Natural Products - Immunomodulators
 
 
ALX-850-307 Revised 29-Apr-05
Chaperone Cocktail
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PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Stress & Heat Shock Proteins Other Products
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ALX-850-307-KI01   1 Kit 590.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli.
QUANTITY: 85µl
CONCENTRATION: 2.4mg/ml total protein.
KIT/SET CONTAINS: Contains the following recombinant E. coli proteins:
ClpB (10µM)
DnaK (10µM)
DnaJ (2µM)
GrpE (1µM)
GroEL (10µM)
GroES (10µM)
PURITY: ≥95%
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 50mM sodium chloride, 5mM magnesium dichloride and 5% (v/v) glycerol.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Description
High power chaperone mix which mediates the ATP-dependent refolding of aggregated proteins (i.e. inclusion bodies).
Product Specific Literature References
Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network: P. Goloubinoff, et al.; PNAS 96, 13732 (1999) Abstract; Full Text
Further Categories Containing This Product:
Panels / Sets
 
 
ALX-201-190 Revised 16-Feb-05
HSBP1 (human) (recombinant)
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SYNONYMS Heat Shock Factor Binding Protein 1 (human) (recombinant)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Stress & Heat Shock Proteins Other Products
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ALX-201-190-C100   100 µg 125.00 USD Add To Cart
Product Specification
MW: ~8.5kDa.
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 20mM TRIS-HCl, pH 7.5, containing 20% glycerol, 50mM NaCl, 1mM DTT.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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General Information
Heat shock factor binding protein 1 (HSBP1) is a 76 amino acid protein that binds to heat shock factor 1 (HSF1), which is a transcription factor involved in the HS response.
HSBP1 is localized in the nucleus and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity.
General Literature References
The heat-shock response: regulation and function of heat-shock proteins and molecular chaperones: R.I. Morimoto, et al.; Essays Biochem. 32, 17 (1997) Abstract
Negative regulation of the heat shock transcriptional response by HSBP1: S.H. Satyal, et al.; Genes Dev. 12, 1962 (1998) Abstract; Full Text
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Recombinant Proteins / Fusion Proteins
 
 
ALX-201-024 Revised 24-Feb-05
HSF1 (human) (recombinant)
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SYNONYMS Heat Shock Factor 1 (human) (recombinant)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Stress & Heat Shock Proteins Other Products
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ALX-201-024-C150   150 µg 343.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli.
FORMULATION: Liquid. Sonicated E. coli extract at a total protein concentration of 6mg/ml in 25mM HEPES, pH 7.9, 12.5mM MgCl, 0.1mM EDTA, 10% glycerol, 1mM DTT, 0.1% NP-40 and 300mM KCl and protease inhibitors.
APPLICATION: Positive control in gel shift assays (25 tests) or Western blot (50 tests) with PAb to HSF1 (Prod. No. ALX-210-129). DNase I footprinting and in vitro transcription assays.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -20°C
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q00613: HSF 1 (human)
Further Categories Containing This Product:
Transcription Factors Other Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-804-495 Revised 22-Dec-06
Monoclonal Antibody to Pin1 (8C10)
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SYNONYMS anti-Peptidyl-prolyl cis-trans Isomerase Pin1 MAb (8C10)
anti-PPIase Pin1 (8C10) MAb
anti-Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 MAb (8C10)
anti-PPIase NIMA-interacting 1 (8C10) MAb
anti-Rotamase Pin1 MAb (8C10)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Alzheimer Research Other Products
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ALX-804-495-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 8C10
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN: Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1.
SPECIFICITY: Recognizes human, mouse and dog Pin1. Detects a band of ~16 kDa by Western blot.
APPLICATION: Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL INCLUDED: Yes (Prod. No. ALX-840-030).
General Information
Pin1, a member of the parvulin family of peptidyl-prolyl-cis-trans-isomerases (PPIases), specifically isomerizes only the phosphorylated  Ser/Thr-Pro bonds in certain proteins like Bcl-2, Cdc25C, Cdc27, p53 and Tau. Binding of Pin1 to p53 stimulates the DNA binding activity and transactivation function of p53. Pin1 also regulates the expression of cyclin D1 and is strikingly overexpressed in a subset of human tumors like breast cancer and oral squamous cell carcinoma.
Further Categories Containing This Product:
Stress & Heat Shock Proteins Other Products • Antiviral Signalling / Related Products • Monoclonal Antibodies • p53 Other Products
 
 
ALX-804-107 Revised 06-Jan-05
Monoclonal Antibody to TCP-1 (91A)
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SYNONYMS anti-T Complex Polypeptide 1 MAb (91A)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Stress & Heat Shock Proteins Other Products
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ALX-804-107-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Dog
Monkey
Others
CLONE: 91A
ISOTYPE: Rat IgG2a
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Recombinant mouse TCP-1 (T complex polypeptide 1) (aa 306-556).
SPECIFICITY: Recognizes an epitope of aa 465-469 of TCP-1 from a broad range of species including mouse, rat, dog, monkey, hamster, chicken and yeast. Detects a band of ~57kDa by Western blot.
APPLICATION: Immunocytochemistry (1:500)
Immunoprecipitation
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-201-221 Revised 02-Oct-06
Pin1 (human) (recombinant)
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SYNONYMS Peptidyl-prolyl cis-trans Isomerase Pin1 (human) (recombinant)
PPIase Pin1 (human) (recombinant)
Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 (human) (recombinant)
PPIase NIMA-interacting 1 (human) (recombinant)
Rotamase Pin1 (human) (recombinant)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Alzheimer Research Other Products
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ALX-201-221-C100   100 µg 325.00 USD Add To Cart
Product Specification
MW: ~18.2kDa
SOURCE/HOST: Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1 produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 20mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 5mM DTT and 20% glycerol.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Stress & Heat Shock Proteins Other Products • Recombinant Proteins / Fusion Proteins • Antiviral Signalling / Related Products • p53 Other Products
 
 
ALX-210-129 Revised 25-Jan-07
Polyclonal Antibody to HSF1
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SYNONYMS anti-Heat Shock Factor 1 PAb
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Stress & Heat Shock Proteins Other Products
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ALX-210-129-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Antiserum diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human HSF1 (heat shock factor 1).
SPECIFICITY: Recognizes human, mouse and rat HSF1. Detects a band of ~83kDa by Western blot.
APPLICATION: Gel Supershift Assay
Immunoprecipitation
Western Blot (1:10'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Transcription Factors Other Products • Polyclonal Antibodies
 
 
ALX-210-141 Revised 10-Jan-05
Polyclonal Antibody to KDEL/GRP78
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PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Stress & Heat Shock Proteins Other Products
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ALX-210-141-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Others
SOURCE/HOST: From rabbit.
FORMULATION: Lyophilized. Purified IgG containing 0.05% sodium azide.
RECONSTITUTION: Reconstitute in PBS.
IMMUNOGEN: Synthetic peptide corresponding to aa 643-654 (CT643GEEDTSEKDEL654) of rat GRP78 (glucose regulated protein 78).
SPECIFICITY: Recognizes mouse, rat  and hamster endoplasmic reticulum (ER) targeting sequence KDEL. Does not cross-react with human endoplasmic reticulum (ER) targeting sequence KDEL.
APPLICATION: Immunocytochemistry
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
ER-Golgi Intermediate Compartment [ERGIC] / Related Products • Polyclonal Antibodies
 
 
ALX-210-919 Revised 14-Jan-08
Polyclonal Antibody to SODD (human) (AL169)
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SYNONYMS anti-Silencer of Death Domain (human) PAb (AL169)
anti-BAG-4 (human) PAb (AL169)
anti-BAG-family Molecular Chaperone Regulator-4 (human) PAb (AL169)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Adaptor Proteins for Death Receptors Other Products
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ALX-210-919-C050   50 µg 194.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to C-terminal aa 350-376 (N350AT
SDHPNNQDQSSSLPEECVPSDEST376) of human SODD (Silencer of Death Domain; BAG-4; BAG-family Molecular Chaperone Regulator-4).
SPECIFICITY: Recognizes human SODD. Detects bands of ~70kDa (full length protein) and ~60kDa (a C-terminal fragment) by Western blot.
APPLICATION: Western Blot (1:1000)
Note: Not recommended for immunoprecipitation.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Apoptosis: Silencing the death receptors: J. Tschopp, et al.; Curr. Biol. 9, R381 (1999) Abstract; Full Text
Overexpression of the 'silencer of death domain', SODD/BAG-4, modulates both TNFR1- and CD95-dependent cell death pathways: H. Eichholtz-Wirth, et al.; Cancer Lett. 194, 81 (2003) Abstract
Further Categories Containing This Product:
Stress & Heat Shock Proteins Other Products • Polyclonal Antibodies
 
 
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