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GTPase-activating Proteins [GAPs]
You are here: Product Lines > Signal Transduction > Ras Signalling Pathway > GTPase-activating Proteins [GAPs]
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ALX-804-020 Revised 11-Jan-05
Monoclonal Antibody to GTPase Activating Protein (B4F8)
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SYNONYMS anti-GAP MAb (B4F8)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY GTPase-activating Proteins [GAPs]
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-020-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Monkey
Others
CLONE: B4F8
ISOTYPE: Mouse IgG2a
FORMULATION: Lyophilized. Protein A purified antibody.
RECONSTITUTION: Reconstitute in PBS.
IMMUNOGEN: Recombinant human GAP (GTPase activating protein).
SPECIFICITY: Recognizes an epitope of human, mouse, rat, bovine and monkey GAP containing the src homology regions SH2 and SH3. Detects a band of ~120kDa by Western blot.
APPLICATION: Immunocytochemistry (5µg/ml)
Immunoprecipitation (1-5µg/ml)
Western Blot (10µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-210-571 Revised 10-Apr-08
Polyclonal Antibody to ARAP1 (human)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY GTPase-activating Proteins [GAPs]
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-571-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
PURITY: Liquid. In PBS.
IMMUNOGEN: Recombinant C-terminal part of mouse ARAP1 (aa 1084-1371).
SPECIFICITY: Recognizes human ARAP1.
APPLICATION: Western Blot (1µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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General Information
The adaptor protein ARAP1 is a PIP3-dependent Arf GAP that regulates Arf-, Rho-, and Cdc42-dependent cell activities. ARAP1 plays an important role in the regulation of blood pressure and renal function via activation of the intrarenal renin-angiotensin system. It was shown that ARAP1 might affect the efficacy of TRAIL-induced apoptosis due to its cell-specific trafficking of TRAIL-R1 (DR4).
General Literature References
ARAP1: a point of convergence for Arf and Rho signaling: K. Miura, et al.; Mol. Cell 9, 109 (2002) Abstract
Development of hypertension and kidney hypertrophy in transgenic mice overexpressing ARAP1 gene in the kidney: D.F. Guo, et al.; Hypertension 48, 453 (2006) Abstract; Full Text
Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane: S. Simova, et al.; Apoptosis 13, 423 (2008) Abstract
Further Categories Containing This Product:
Renin-Angiotensin-Aldosterone System [RAAS] Other Products • Polyclonal Antibodies
 
 
ALX-210-781 Revised 16-Feb-05
Polyclonal Antibody to RasGAP (CT)
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SYNONYMS anti-Ras-specific GTPase-activating Protein (CT) PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY GTPase-activating Proteins [GAPs]
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ALX-210-781-R100   100 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Monkey
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. 100µl serum containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1034-1047 of human RasGAP (Ras-specific GTPase-activating protein).
SPECIFICITY: Recognizes human, mouse, rat and monkey full-length RasGAP as well as the C-terminal (CT) fragment generated by caspase cleavage (RasGAP aa 456-1047). Detects a band of ~150 kDa by Western blot. Does not cross-react with the N-terminal portion of RasGAP (including the SH2 and SH3 domains).
APPLICATION: Immunoprecipitation (1:1'000; only CT RasGAP; see technical note)
Western Blot (1:1'000-1:3'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
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Product Description
RasGAP
RasGAP (Ras-specific GTPase-activating protein) is one of the few signalling proteins cleaved by caspases during apoptosis [1-3]. The kinetics of RasGAP cleavage is identical to well-known caspase substrates such as PARP or the caspase themselves. RasGAP cleavage by caspases generates a carboxy-terminal fragment of about 64 kDa (fragment C) containing the GAP activity and a N-terminal fragment (fragment N) of about 56 kDa. The latter fragment can be further cleaved into two other smaller fragments, one of which contains the SH2 and SH3 domains. The fact that RasGAP is an abundant ubiquitous protein readily cleaved during apoptosis makes it a very good apoptotic marker.
RasGAP is a unique caspase substrate because it can either inhibit or promote apoptosis depending on the extent of its cleavage by caspases. At low levels of caspase activity, generation of fragment N induces a potent Ras-PI3K-Akt-dependent survival pathway. At higher levels of caspase activity, fragment N is further cleaved into two fragments (N1 and N2) that potentiate apoptosis. RasGAP has recently been qualified as an “apoptostat” because it functions as a sensor of caspase activity to determine whether or not a cell should survive [2].
Product Specific Literature References
[1] Caspase-dependent cleavage of signaling proteins during apoptosis. A turn-off mechanism for anti-apoptotic signals: C. Widmann, et al.; J. Biol. Chem. 273, 7141 (1998) Abstract; Full Text
[2] Antiapoptotic signaling generated by caspase-induced cleavage of RasGAP: J.-Y. Yang, et al.; Mol. Cell. Biol. 21, 5346 (2002) Abstract; Full Text
[3] The RasGAP N-terminal fragment generated by caspase cleavage protects cells in a Ras/PI3K/Akt-dependent manner that does not rely on NF-κB activation: J.-Y. Yang, et al.; J. Biol. Chem. 277, 14641 (2002) Abstract; Full Text
Ras GTPase-activating protein binds to Akt and is required for its activation: Y. Yue, et al.; J. Biol. Chem. 279, 12883 (2004) Abstract; Full Text
RasGTPase-activating protein is a target of caspases in spontaneous apoptosis of lung carcinoma cells and in response to etoposide: B. Bartling, et al.; Carcinogenesis 25, 909 (2004) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Technical note: Due to the conformation of native full-length RasGAP, steric hinderance prevents binding of this antibody to the C-terminus. Thus immunoprecipitation is possible only with the C-terminal RasGAP fragment and not with the full-length protein.

The C-terminal (aa 456-1047) fragment contains the GTPase-activating protein activity of RasGAP as well as a C2 domain and a PH domain.
Further Categories Containing This Product:
Apoptosis & Necrosis Detection Other Products • Polyclonal Antibodies
 
 
ALX-210-860 Revised 31-Oct-06
Polyclonal Antibody to RasGAP (NT)
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SYNONYMS anti-Ras-specific GTPase-activating Protein (NT) PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY GTPase-activating Proteins [GAPs]
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ALX-210-860-R100   100 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: Affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RasGAP (Ras-specific GTPase-activating protein) fragment N2 (aa 158-455).
SPECIFICITY: Recognizes human, mouse and rat full-length RasGAP as well as the N-terminal fragment (that comprises SH2 and SH3 domains) generated by caspase cleavage (fragments N and N2). Does not cross-react with other GAP proteins.
APPLICATION: Immunoprecipitation
Western Blot (1:1'000-1:3'000 for detection of the full-length protein; 1:100-1:300 for detection of the caspase-generated fragments).
Other applications not tested.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
[1] Antiapoptotic signaling generated by caspase-induced cleavage of RasGAP: J.-Y. Yang, et al.; Mol. Cell. Biol. 21, 5346 (2001) Abstract; Full Text
[2] RasGTPase-activating protein is a target of caspases in spontaneous apoptosis of lung carcinoma cells and in response to etoposide: B. Bartling, et al.; Carcinogenesis 25, 909 (2004) Abstract
General Information
RasGAP (Ras-specific GTPase-activating protein) is one of the few signalling proteins cleaved by caspases during apoptosis [1,3,4]. The kinetics of RasGAP cleavage is identical to well-known caspase substrates such as PARP or the caspase themselves. RasGAP cleavage by caspases generates a C-terminal fragment of about 64kDa (fragment C) containing the GAP activity and a N-terminal fragment (fragment N) of about 56kDa. The latter fragment can be further cleaved into two other smaller fragments, one of which contains the SH2 and SH3 domains. The fact that RasGAP is an abundant ubiquitous protein readily cleaved during apoptosis makes it a very good apoptotic marker.
RasGAP is a unique caspase substrate because it can either inhibit or promote apoptosis depending on the extent of its cleavage by caspases. At low levels of caspase activity that do not induce apoptosis, generation of fragment N induces a potent Ras-PI3K-Akt-dependent survival pathway [5]. The formation of fragment N is mandatory for survival of stressed cells. The presence of fragment N, but not of fragment N2, in cells is indicative of mild caspase-3 activity. At higher levels of caspase activity, fragment N is further cleaved into two fragment (N1 and N2) that potentiate apoptosis. The presence of fragment N2 in cells is indicative of high caspase-3 activity. RasGAP has recently been qualified as an “apoptostat” because it functions as a sensor of caspase activity to determine whether or not a cell should survive [1].
General Literature References
[3] Caspase-dependent cleavage of signaling proteins during apoptosis. A turn-off mechanism for anti-apoptotic signals: C. Widmann, et al.; J. Biol. Chem. 273, 7141 (1998) Abstract; Full Text
[4] Proteolytic cleavage of ras GTPase-activating protein during apoptosis: L.P. Wen, et al.; Cell Death Differ. 5, 729 (1998) Abstract
[5] The RasGAP N-terminal fragment generated by caspase cleavage protects cells in a Ras/PI3K/Akt-dependent manner that does not rely on NFkappa B activation: J.Y. Yang and C. Widmann; J. Biol. Chem. 277, 14641 (2002) Abstract; Full Text
Further Categories Containing This Product:
Apoptosis & Necrosis Detection Other Products • Polyclonal Antibodies
 
 
ALX-210-269 Revised 13-Jan-05
Polyclonal Antibody to SynGAP
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SYNONYMS anti-Synaptic Ras-GTPase Activating Protein PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY GTPase-activating Proteins [GAPs]
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-269-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 947-1167 of rat SynGAP (synaptic Ras-GTPase activating protein).
SPECIFICITY: Recognizes mouse and rat SynGAP. Detects a band of ~135kDa by Western blot.
APPLICATION: Immunocytochemistry (1µg/ml)
Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Product Description
SynGAP, synaptic Ras-GTPase activating protein, is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with PSD-95, a synaptic scaffold protein, and synaptic NMDA receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
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