ALEXIS Biochemicals: Monoclonal Antibody to Phospholamban (2D12)

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ALX-201-259

Revised 26-Feb-07

Recoverin (human) (recombinant)
SYNONYMS	CAR (human) (recombinant) Cancer-associated Retinopathy Protein (human) (recombinant)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calcium Signalling (Intracellular) Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-259-C100		100 µg	430.00 USD

Product Specification

MW:	~23kDa
SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 20mM TRIS, pH 8.0, containing 1mM EDTA, 2mM MgCl₂ and 10% glycerol.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Cloning, expression, and crystallization of recoverin, a calcium sensor in vision: S. Ray, et al.; PNAS 89, 5705 (1992) Abstract; Full Text
The effect of recombinant recoverin on the photoresponse of truncated rod photoreceptors: M.A. Erickson, et al.; PNAS 95, 6474 (1998) Abstract; Full Text
Cerebrospinal fluid (vascular endothelial growth factor) and serologic (recoverin) tumor markers for malignant glioma: P. Sampath, et al.; Cancer Control 11, 174 (2004) Abstract; Full Text

Further Categories Containing This Product:

Vision Research • Recombinant Proteins / Fusion Proteins • Cancer Other Products

ALX-201-383

Revised 18-Mar-08

Nucleobindin-2 (mouse) (recombinant) (His)
SYNONYMS	NUCB2 (mouse) (recombinant) (His) DNA-binding Protein NEFA (mouse) (recombinant) (His) Nesfatin (mouse) (recombinant) (His)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calcium Signalling (Intracellular) Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-383-C010		10 µg	350.00 USD
ALX-201-383-C050		50 µg	490.00 USD

Product Specification

SOURCE/HOST:	Produced in E. coli. Mature human nucleobindin-2 (aa 25-420) is fused at the C-terminus to a His-tag.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. 0.2µm-filtered solution in PBS, pH 7.2.
ENDOTOXIN CONTENT:	<1EU/µg protein (LAL-test).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

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General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P81117: Nucleobindin-2 (mouse) [Precursor]
MANUFACTURER	Manufactured by AdipoGen, Inc.

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ALX-210-290

Revised 11-May-07

Polyclonal Antibody to Calsequestrin
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calcium Signalling (Intracellular) Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-290-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Sheep Dog
SOURCE/HOST:	From rabbit.
FORMULATION:	Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:	Purified dog cardiac calsequestrin.
SPECIFICITY:	Recognizes human, mouse, rat, dog and sheep calsequestrin. Recognizes both cardiac and skeletal muscle calsequestrin. Detects a band of ~55kDa by Western blot.
CROSSREACTIVITY PROTEINS:	Additional bands at ~97kDa may be observed and have been reported to be calsequestrin-like proteins.
APPLICATION:	Immunoprecipitation Western Blot (1:2'500)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Developmental changes in cardiac sarcoplasmic reticulum in sheep: L. Mahony & L.R. Jones; J. Biol. Chem. 261, 15257 (1986) Abstract; Full Text
Complex formation between junctin, triadin, calsequestrin, and the ryanodine receptor. Proteins of the cardiac junctional sarcoplasmic reticulum membrane: L. Zhang, et al.; J. Biol. Chem. 272, 23389 (1997) Abstract; Full Text
Identification of triadin 1 as the predominant triadin isoform expressed in mammalian myocardium: Y.M. Kobayashi & L.R. Jones; J. Biol. Chem. 274, 28660 (1999) Abstract; Full Text

General Information

The sarcoplasmic reticulum (SR) is in part responsible for maintaining the level of intracellular Ca²⁺ in cardiac and skeletal muscle by storing and releasing Ca²⁺. Several intralumenal SR Ca²⁺ binding proteins have been identified, the most prominent of these is calsequestrin. Calsequestrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize Ca²⁺ near the junctional face of the terminal cisternae from which Ca²⁺ can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for Ca²⁺. Two mammalian isoforms of calequestrin exist, a cardiac and a skeletal muscle isoform, which are the products of different genes and are 63% identical. This antibody recognizes both cardiac and skeletal muscle calsequestrin from a variety of species.

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Polyclonal Antibodies

ALX-210-292

Revised 13-Jan-05

Polyclonal Antibody to Na+/Ca2+/K+Exchanger (rat)
SYNONYMS	anti-NCKX2 (rat) PAb
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calcium Signalling (Intracellular) Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-292-R200		200 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Rat
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml.
FORMULATION:	Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 90-102 (D⁹⁰LNDKIRDYTPQP¹⁰²C) of rat NCKX2 (Na⁺/ Ca²⁺/K⁺ exchanger).
SPECIFICITY:	Recognizes rat NCKX2. Detects a band of ~78kDa by Western blot.
APPLICATION:	Immunohistochemistry (frozen sections (1:100)) Western Blot (1:100)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-163-008.

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Product Description

Na⁺/Ca²⁺-K⁺ exchanger proteins (NCKX1) have been reported to occur in retinal photoreceptors and in brain (NCKX2). Unlike the abundant and widely expressed Na⁺/Ca²⁺ exchanger which catalyzes the exchange of 3 Na⁺ ions for 1 Ca²⁺ ion to modulate intracellular Ca²⁺ levels, NCKX isoforms exchange 4 Na⁺ ions for 1 Ca²⁺ and 1 K⁺ ion. The Na⁺/Ca²⁺-K⁺ exchanger uses the transmembrane Na⁺ gradient to catalyze countertransport of Ca²⁺ in a K⁺-dependent mechanism against its electrochemical gradient. It is hypothesized that NCKX2 plays a significant role in calcium homeostasis and the regulation of neuronal function.

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Polyclonal Antibodies

ALX-210-460

Revised 26-Apr-07

Polyclonal Antibody to Nucleobindin-2 (mouse)
SYNONYMS	anti-NUCB2 (mouse) PAb anti-DNA-binding Protein NEFA (mouse) PAb anti-Nesfatin (mouse) PAb
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calcium Signalling (Intracellular) Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-460-C100		100 µg	310.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml.
PURITY DETAIL:	Purified IgG.
FORMULATION:	Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:	Recombinant mouse nucleobindin-2 (NUCB2).
SPECIFICITY:	Recognizes mouse nucleobindin-2.
APPLICATION:	ELISA (direct and indirect: 1:2’000-1:5’000) Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 1-2 weeks when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

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General Information

MANUFACTURER

Manufactured by AdipoGen, Inc.

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ALX-350-105

Revised 05-Apr-08

Hellebrin
SYNONYMS	3β-(O4-β-D-Glucopyranosyl-α-L-rhamnopyranosyloxy)-5,14-dihydroxy-19-oxo-5β,14-β-bufa-20,22-dienolide
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products - ATPase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-105-M001		1 mg	45.00 USD
ALX-350-105-M005		5 mg	180.00 USD

Product Specification

FORMULA:	C₃₆H₅₂O₁₅
MW:	724.8
CAS NUMBER:	13289-18-4
SOURCE/HOST:	Isolated from Helleborus sp.
PURITY:	≥99%
APPEARANCE:	White solid.
SOLUBILITY:	Soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HAZARD:	TOXIC.

Product Description

Cardiotonic glycoside which presents a bufadienolide-steroid structure. Besides ouabain (Prod. No. ALX-350-066), from the class of cardenolides hellebrin is the second unique water soluble cardiotonic glycoside. Extremely potent inhibitor of Na⁺/K⁺-ATPase (sodium pump) blocking the active efflux of Na⁺ and reuptake of K⁺ of this membrane enzyme. Shows a positive inotropic effect by increasing the intracellular Ca²⁺ concentration. Strong immunosuppressor inhibiting T cell activity with much higher potency than cortisol or cyclosporin A (Prod. No. ALX-380-002). Induces caspase-dependent apoptosis.

Product Specific Literature References

Über Hellebrin, ein krystallisiertes Glykosid aus Radix Hellebori nigri: W. Karrer; Helv. Chim. Acta 26, 1353 (1943)
The structure of hellebrin: P. Muhr, et al.; Liebigs Ann. Org. Bioorg. Chem. 2, 443 (1995)
In search of ideal inotropic steroids: recent progress: K.R. Repke, et al.; Prog. Drug Res. 47, 9 (1996) Abstract
Exquisitely small amounts of nonglucocorticoid natural steroids suppress the human allogeneic T-cell response: P. Terness, et al.; Transplant Proc. 33, 547 (2001) Abstract
Apoptosis-mediated selective killing of malignant cells by cardiac steroids: maintenance of cytotoxicity and loss of cardiac activity of chemically modified derivatives: D. Daniel, et al.; Int. Immunopharmacol. 3, 1791 (2003) Abstract

Further Categories Containing This Product:

Na+ / K+-ATPase / Related Products • Calcium Signalling (Intracellular) Other Products • Natural Products - Immunomodulators • Natural Products - Apoptosis Inducers & Inhibitors

ALX-350-234

Revised 08-Apr-08

12-epi-Scalaradial
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products - Other Signal Transduction Pathway Modulators

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-234-M001		1 mg	190.00 USD

Product Specification

FORMULA:	C₂₇H₄₀O₄
MW:	428.6
CAS NUMBER:	53527-28-9
SOURCE/HOST:	Isolated from Cacospongia sp.
PURITY:	≥98%
APPEARANCE:	Waxy solid.
SOLUBILITY:	Soluble in acetic acid, 100% ethanol or DMSO.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Inhibitor of bee venom phospholipase A₂(PLA₂). Inhibits Ca²⁺ mobilization induced by leukotriene B₄ (LTB₄) and platelet activating factor. Inhibits Ca²⁺-dependent arachidonic acid release in human neutrophils. Inhibits epidermal growth factor (EGF)-stimulated Akt (protein kinase B; PKB) phosphorylation.

Product Specific Literature References

Two-step inactivation of bee venom phospholipase A2 by scalaradial: M.S. De Carvalho & R.S. Jacobs; Biochem. Pharmacol. 42, 1621 (1991) Abstract
Evidence for different mechanisms involved in the formation of lyso platelet-activating factor and the calcium-dependent release of arachidonic acid from human neutrophils: J.D. Winkler, et al.; Biochem. Pharmacol. 44, 2055 (1992) Abstract
Effects of scalaradial, a type II phospholipase A2 inhibitor, on human neutrophil arachidonic acid mobilization and lipid mediator formation: L.A. Marshall, et al.; J. Pharmacol. Exp. Ther. 268, 709 (1994) Abstract
Scalaradial inhibition of epidermal growth factor receptor-mediated Akt phosphorylation is independent of secretory phospholipase A2: Y. Xie, et al.; J. Pharmacol. Exp. Ther. 314, 1210 (2005) Abstract
Scalaradial, a dialdehyde-containing marine metabolite that causes an unexpected noncovalent PLA2 Inactivation: M.C. Monti, et al.; Chembiochem 8, 1585 (2007) Abstract

Further Categories Containing This Product:

PLA2 Inhibitors • Akt [PKB] / Related Products • Calcium Signalling (Intracellular) Other Products

ALX-350-362

Revised 26-Mar-08

(+)-Egenine
SYNONYMS	6-(6-Methyl-5,6,7,8-tetrahydro[1,3]dioxolo[4,5-g]isoquinolin-5-yl)-6,8-dihydrofuro[3,4-E][1,3]benzodioxol-8-ol
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products - Other Signal Transduction Pathway Modulators

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-362-M025		25 mg	95.00 USD
ALX-350-362-M100		100 mg	285.00 USD

Product Specification

FORMULA:	C₂₀H₁₉NO₆
MW:	369.4
CAS NUMBER:	6883-44-9
SOURCE/HOST:	Isolated from Fumaria vaillantii or Corydalis decumbens.
PURITY:	≥98% (HPLC)
APPEARANCE:	White to off-white crystalline solid.
SOLUBILITY:	Soluble in ethyl acetate or chloroform; sparingly soluble in 100% ethanol or ether; practically insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

First phthalideisoquinoline hemiacetal from a natural source. Inhibits Ca²⁺ currents and contractile responses in vitro.

Product Specific Literature References

Egenine: A possible intermediate in phthalideisoquinoline biogenesis: B. Gotzler, et al.; Tetrahedron 39, 577 (1983)
Synthesis of (-)-egenine (decumbensine) by asymmetric carbonyl addition: K.S. Rein & R.E. Gawley; Tetrahedron Lett. 31, 3711 (1990)
Synthesis of phthalide isoquinoline alkaloides (-)-egenine, (-)-corytensine, and (-)-bicuculline by asymmetric carbonyl addition of chiral dipole-stabilized organometallics: K.S. Rein & R.E. Gawley; J. Org. Chem. 56, 1564 (1991)
β-Phenylethylamines and the isoquinoline alkaloids: K.W. Bentley; Natural Prod. Rep. 11, 555 (1994), (Review)
1-Magnesiotetrahydroisoquinolyloxazolines as chiral nucleophiles in stereoselective additions to aldehydes: Auxiliary optimization, asymmetric synthesis of (+)-corlumine, (+)-bicuculline, (+)-egenine, and (+)-corytensine, and preliminary (13)C NMR studies: R.E. Gawley & P. Zhang; J. Org. Chem. 61, 8103 (1996) Abstract
Effects of alkaloids from Corydalis decumbens on contraction and electrophysiology of cardiac myocytes: S. Kadota, et al.; Phytother. Res. 10, 18 (1998)

Further Categories Containing This Product:

Calcium Signalling (Intracellular) Other Products • Alkaloids

ALX-350-382

Revised 13-Aug-08

Xestospongin C
SYNONYMS	XeC Araguspongine E
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products - Other Signal Transduction Pathway Modulators

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-382-C100		100 µg	490.00 USD

Product Specification

FORMULA:	C₂₈H₅₀N₂O₂
MW:	446.7
CAS NUMBER:	88903-69-9
SOURCE/HOST:	Isolated from an Okinawan marine sponge Xestospongia sp.
PURITY:	≥90% (HPLC)
APPEARANCE:	White to off-white powder.
FORMULATION:	Lyophilized from 20mM HEPES, pH 7.3, containing 0.1% BSA.
SOLUBILITY:	Soluble in ethyl acetate, acetone or 100% ethanol; slightly soluble in DMSO or water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. Packaged under inert gas.

Product Description

Potent, selective, reversible and membrane-permeable inhibitor of the inositol 1,4,5-triphosphate (IP₃) receptor (IC₅₀=350nM). Inhibits IP₃receptor-mediated Ca²⁺ release. Has vasodilatory properties.

Product Specific Literature References

Structures of xestospongin A,B,C, and D, novel vasodilative compounds from marine sponge, Xestospongia exigua: M. Nakagawa, et al.; THL 25, 3227 (1984)
Xestospongins: potent membrane permeable blockers of the inositol 1,4,5-trisphosphate receptor: J. Gafni, et al.; Neuron 19, 723 (1997) Abstract
New developments in the molecular pharmacology of the myo-inositol 1,4,5-trisphosphate receptor: R. A. Wilcox, et al.; TIPS 19, 467 (1998) Abstract
Isomerization of dimeric 2,9-disubstituted 1-oxa-quinolizidine alkaloids and structural revision of aragusponjines B and E, isolated from a marine sponge of Xesospongia sp.: M. Kobayashi, et al.; Heterocycles 47, 195 (1998)
Xestospongin C is an equally potent inhibitor of the inositol 1,4,5-trisphosphate receptor and the endoplasmic-reticulum Ca(2+) pumps: P. De Smet, et al.; Cell Calcium 26, 9 (1999) Abstract
Xestospongin C, a selective and membrane-permeable inhibitor of IP(3) receptor, attenuates the positive inotropic effect of alpha-adrenergic stimulation in guinea-pig papillary muscle: S. Miyamoto, et al.; Br. J. Pharmacol. 130, 650 (2000) Abstract; Full Text
In vivo signal transduction of nociceptive response by kyotorphin (tyrosine-arginine) through Galpha(i)- and inositol trisphosphate-mediated Ca(2+) influx: H. Ueda & M. Inoue; Mol. Pharmacol. 57, 108 (2000) Abstract; Full Text
Ca2+ entry mediated by store depletion, S-nitrosylation, and TRP3 channels. Comparison of coupling and function: D.B. van Rossum, et al.; J. Biol. Chem. 275, 28562 (2000) Abstract; Full Text
Xestospongin C is a potent inhibitor of SERCA at a vertebrate synapse: A. Castonguay & R. Robitaille; Cell Calcium 32, 39 (2002) Abstract
Inhibitory mechanism of xestospongin-C on contraction and ion channels in the intestinal smooth muscle: H. Ozaki, et al.; Br. J. Pharmacol. 137, 1207 (2002) Abstract; Full Text
Xestospongin C empties the ER calcium store but does not inhibit InsP3-induced Ca2+ release in cultured dorsal root ganglia neurones: N. Solovyova, et al.; Cell Calcium 21, 49 (2002) Abstract

Further Categories Containing This Product:

Inositols & Inositolphosphates / Related Products • Calcium Signalling (Intracellular) Other Products • Marine Natural Products

ALX-804-089

Revised 27-Mar-08

Monoclonal Antibody to Calsequestrin (VIIID12)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calcium Signalling (Intracellular) Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-089-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse Rat Dog Others
CLONE:	VIIID12
ISOTYPE:	Mouse IgG2b
FORMULATION:	Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:	Purified rabbit skeletal muscle sarcoplasmic reticulum.
SPECIFICITY:	Recognizes mouse, rat, rabbit and dog type I (slow) and type II (fast) calsequestrin. Detects a band of ~63kDa by Western blot.
APPLICATION:	Immunocytochemistry Western Blot (1:1’000)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

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