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Items 11 of 11
ALX-804-622
Revised 23-Apr-08
Monoclonal Antibody to Fibulin-1 (human) (MEM-2)
SYNONYMS
anti-Basement-membrane Protein 90 (human) MAb (MEM-2)
anti-BM-90 (human) MAb (MEM-2)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Cytoskeleton & Extracellular Matrix Other Products
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Format:
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ALX-804-622-C050
50 µg
210.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-2
ISOTYPE:
Mouse IgM
CONCENTRATION:
FORMULATION:
Liquid. In 20mM TRIS, pH 7.4, containing 1.25M sodium chloride and 0.02% sodium azide.
IMMUNOGEN:
Recombinant human fibulin-1.
SPECIFICITY:
Recognizes human fibulin-1.
APPLICATION:
Flow Cytometry
Immunohistochemistry (frozen sections; paraffin sections)
Immunoprecipitation
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
HANDLING:
Do not freeze.
Product Specific Literature References
Monoclonal antibody to fibulin-1 generated by genetic immunization:
S.M. Pupa, et al.; J. Cell. Biochem.
89
, 647 (2003)
Abstract
Immunological and pathobiological roles of fibulin-1 in breast cancer:
S.M. Pupa, et al.; Oncogene
23
, 2153 (2004)
Abstract
General Information
The fibulins are a family of secreted glycoproteins that are components of elastic matrix fibers and basement membranes. Fibulin-1 is an extracellular matrix protein, which can adhere to endothelial cells and regulate their motility and proliferation. Fibulin-1 is overexpressed in estrogen receptor (ER)-positive ovarian carcinomas and several studies suggest a possible role for fibulin-1 as a tumor suppressor. Xie et al. described that fibulin-1 inhibits tumor growth in HT1080 cell lines by suppressing tumor angiogenesis and inducing apoptosis.
General Literature References
Basement membrane derived fibulin-1 and fibulin-5 function as angiogenesis inhibitors and suppress tumor growth:
L. Xie, et al.; Exp. Biol. Med. (Maywood)
233
, 155 (2008)
Abstract
Further Categories Containing This Product:
Tumor Suppressors / Related Products
•
Monoclonal Antibodies
ALX-804-254
Revised 14-Aug-07
Monoclonal Antibody to PTEN (6H2.1)
SYNONYMS
anti-Phosphatase and Tensin Homolog Deleted on Chromosome 10 MAb (6H2.1)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
PTEN / Related Products
Ordering Information
Product Numbers:
Format:
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ALX-804-254-C100
100 µg
480.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE:
6H2.1
ISOTYPE:
Mouse IgG
CONCENTRATION:
1mg/ml.
PURITY DETAIL:
Protein A-affinity purified. Sterile filtered.
FORMULATION:
Liquid. In PBS.
IMMUNOGEN:
Recombinant human PTEN.
SPECIFICITY:
Recognizes human, mouse and rat PTEN (epitope aa 304-403). Detects a band of ~60kDa by Western blot.
APPLICATION:
ELISA
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation (5µg)
Western Blot (1-5µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
POSITIVE CONTROL:
For Positive Control in Western blot see Prod. No.
ALX-201-079
.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Immunohistochemical evidence of loss of PTEN expression in primary ductal adenocarcinomas of the breast:
A. Perren, et al.; Am. J. Pathol.
155
, 1253 (1999)
Abstract
;
Full Text
PTEN suppresses breast cancer cell growth by phosphatase activity- dependent G1 arrest followed by cell death:
L.P. Weng, et al.; Cancer Res.
59
, 5808 (1999)
Abstract
;
Full Text
Altered PTEN expression as a diagnostic marker for the earliest endometrial precancers:
G.L. Mutter, et al.; J. Natl. Cancer Inst.
92
, 924 (2000)
Abstract
Changes in endometrial PTEN expression throughout the human menstrual cycle
:
G.L. Mutter, et al.; J. Clin. Endocrinol. Metab.
85
, 2334 (2000)
Abstract
;
Full Text
Molecular identification of latent precancers in histologically normal endometrium
:
G.L. Mutter, et al.; Cancer Res.
61
, 4311 (2001)
Abstract
;
Full Text
Further Categories Containing This Product:
Tumor Suppressors / Related Products
•
Monoclonal Antibodies
ALX-804-451
Revised 14-Aug-07
Monoclonal Antibody to PTEN (11G8.1)
SYNONYMS
anti-Phosphatase and Tensin Homolog Deleted on Chromosome 10 MAb (11G8.1)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
PTEN / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-451-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
11G8.1
ISOTYPE:
Mouse IgG
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. Protein A purified antibody, sterile filtered in PBS.
IMMUNOGEN:
Recombinant human PTEN.
SPECIFICITY:
Recognizes human and mouse PTEN (epitope aa 152-200). Detects a band of ~60 kDa by Western blot.
APPLICATION:
Immunoprecipitation (5µg/sample)
Western Blot (1-5µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
POSITIVE CONTROL:
For Positive Control in Western blot see Prod. No.
ALX-201-079
.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
Tumor Suppressors / Related Products
•
Monoclonal Antibodies
ALX-803-318
Revised 24-Apr-08
Monoclonal Antibody to RUNX3 (human) (R3-6E9)
SYNONYMS
anti-Runt-related Transcription Factor 3 (human) MAb (R3-6E9)
anti-CBF-α3 (human) MAb (R3-6E9)
anti-Core-binding Factor α3 Subunit (human) MAb (R3-6E9)
anti-AML-2 (human) MAb (R3-6E9)
anti-Acute Myeloid Leukemia 2 Protein (human) MAb (R3-6E9)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-803-318-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
R3-6E9
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human RUNX3 (runt-related transcription factor 3) (aa 191-300).
SPECIFICITY:
Recognizes human RUNX3. Weakly cross-reacts with mouse RUNX3.
APPLICATION:
Immunohistochemistry (paraffin sections (2µg/ml)) (see [1])
Immunoprecipitation
Western Blot (0.2µg/ml (see [1]))
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
[1]
RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization:
K. Ito, et al.; Cancer Res.
65
, 7743 (2005)
Abstract
;
Full Text
[2]
RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer:
Q.C. Lau, et al.; Cancer Res.
66
, 6512 (2006)
Abstract
;
Full Text
[3]
Targets of genome copy number reduction in primary breast cancers identified by integrative genomics:
W. Chen, et al.; Genes Chromosomes Cancer
46
, 288 (2007)
Abstract
General Information
RUNX (Runt-related transcription factor) family members play important roles in normal developmental processes and carcinogenesis. Of the three known RUNX family members, the ubiquitously expressed RUNX3 is involved in neurogenesis, T cell differentiation, tumorigenesis and is a component of the TGF-β induced tumor suppressor pathway. In gastric cancer cells, RUNX3 induces cell cycle arrest and apoptosis through upregulation of Bim, a proapoptotic BH3-only protein. In addition, RUNX3 is frequently inactivated in gastric cancer cells.
General Literature References
Tumor suppressor activity of RUNX3:
S.C. Bae & J.K. Choi; Oncogene
23
, 4336 (2004)
Abstract
The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing transforming growth factor beta-induced apoptosis:
T. Yano, et al.; Mol. Cell. Biol.
26
, 4474 (2006)
Abstract
;
Full Text
Further Categories Containing This Product:
Tumor Suppressors / Related Products
•
Monoclonal Antibodies
ALX-803-319
Revised 24-Apr-08
Monoclonal Antibody to RUNX3 (human) (R3-5G4)
SYNONYMS
anti-Runt-related Transcription Factor 3 (human) MAb (R3-5G4)
anti-CBF-α3 (human) MAb (R3-5G4)
anti-Core-binding Factor α3 Subunit (human) MAb (R3-5G4)
anti-AML-2 (human) MAb (R3-5G4)
anti-Acute Myeloid Leukemia 2 Protein (human) MAb (R3-5G4)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-803-319-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
R3-5G4
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human RUNX3 (runt-related transcription factor 3) (aa 191-300).
SPECIFICITY:
Recognizes human RUNX3. Weakly cross-reacts with mouse RUNX3.
APPLICATION:
Immunoprecipitation
Western Blot (0.2µg/ml (see [1]))
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
[1]
RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization:
K. Ito, et al.; Cancer Res.
65
, 7743 (2005)
Abstract
;
Full Text
General Information
RUNX (Runt-related transcription factor) family members play important roles in normal developmental processes and carcinogenesis. Of the three known RUNX family members, the ubiquitously expressed RUNX3 is involved in neurogenesis, T cell differentiation, tumorigenesis and is a component of the TGF-β induced tumor suppressor pathway. In gastric cancer cells, RUNX3 induces cell cycle arrest and apoptosis through upregulation of Bim, a proapoptotic BH3-only protein. In addition, RUNX3 is frequently inactivated in gastric cancer cells.
General Literature References
Tumor suppressor activity of RUNX3:
S.C. Bae & J.K. Choi; Oncogene
23
, 4336 (2004)
Abstract
RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer:
Q.C. Lau, et al.; Cancer Res.
66
, 6512 (2006)
Abstract
;
Full Text
The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing transforming growth factor beta-induced apoptosis:
T. Yano, et al.; Mol. Cell. Biol.
26
, 4474 (2006)
Abstract
;
Full Text
Further Categories Containing This Product:
Tumor Suppressors / Related Products
•
Monoclonal Antibodies
ALX-803-320
Revised 24-Apr-08
Monoclonal Antibody to RUNX3 (R3-1E10)
SYNONYMS
anti-Runt-related Transcription Factor 3 MAb (R3-1E10)
anti-CBF-α3 MAb (R3-1E10)
anti-Core-binding Factor α3 Subunit MAb (R3-1E10)
anti-AML-2 MAb (R3-1E10)
anti-Acute Myeloid Leukemia 2 Protein MAb (R3-1E10)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-803-320-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
R3-1E10
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human RUNX3 (runt-related transcription factor 3) (aa 191-300).
SPECIFICITY:
Recognizes human and mouse RUNX3.
APPLICATION:
Immunohistochemistry (paraffin sections (2µg/ml) (see [1]))
Western Blot (0.2µg/ml (see [1]))
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
[1]
The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing transforming growth factor beta-induced apoptosis:
T. Yano, et al.; Mol. Cell. Biol.
26
, 4474 (2006)
Abstract
;
Full Text
General Information
RUNX (Runt-related transcription factor) family members play important roles in normal developmental processes and carcinogenesis. Of the three known RUNX family members, the ubiquitously expressed RUNX3 is involved in neurogenesis, T cell differentiation, tumorigenesis and is a component of the TGF-β induced tumor suppressor pathway. In gastric cancer cells, RUNX3 induces cell cycle arrest and apoptosis through upregulation of Bim, a proapoptotic BH3-only protein. In addition, RUNX3 is frequently inactivated in gastric cancer cells.
General Literature References
Tumor suppressor activity of RUNX3:
S.C. Bae & J.K. Choi; Oncogene
23
, 4336 (2004)
Abstract
RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer:
Q.C. Lau, et al.; Cancer Res.
66
, 6512 (2006)
Abstract
;
Full Text
Further Categories Containing This Product:
Tumor Suppressors / Related Products
•
Monoclonal Antibodies
ALX-804-579
Revised 18-Jun-08
Monoclonal Antibody to SEL1L (MSel1)
SYNONYMS
anti-Suppressor of lin-12-like Protein MAb (MSel1)
PRODUCT LINE
Stress & Heat Shock Proteins
PRODUCT CATEGORY
Endoplasmatic Reticulum Stress
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-579-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
MSel1
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In 150mM sodium chloride, pH 7.4, containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human SEL1L (suppressor of lin-12-like protein).
SPECIFICITY:
Recognizes human and mouse SEL1L.
APPLICATION:
Immunohistochemistry (frozen sections; paraffin sections)
Immunoprecipitation
Western Blot (10µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
HANDLING:
Do not freeze.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Production of a monoclonal antibody directed against the recombinant SEL1L protein
:
R. Orlandi, et al.; J. Biol. Markers
17
, 104 (2002)
Abstract
SEL1L expression decreases breast tumor cell aggressiveness in vivo and in vitro
:
R. Orlandi, et al.; Cancer Res.
62
, 567 (2002)
Abstract
;
Full Text
General Information
SEL1L (suppressor of lin-12-like protein) is the human ortholog of the
C. elegans
sel-1 gene, a negative regulator of the lin-12/Notch family of proteins. Human SEL1L is a component of endoplasmic reticulum-associated degradation (ERAD), involved in ubiquitin-dependent degradation of misfolded ER proteins. In addition, SEL1L plays a role in cancer progression. Several studies provided significant evidence that increased SEL1L expression is linked to a decrease in tumor aggressiveness.
General Literature References
Complete cDNA sequence and genomic organization of a human pancreas-specific gene homologous to Caenorhabditis elegans sel-1:
Y. Harada, et al.; J. Hum. Genet.
44
, 330 (1999)
Abstract
SEL1L a multifaceted protein playing a role in tumor progression:
I. Biunno, et al.; J. Cell Physiol.
208
, 23 (2006)
Abstract
A different pathway in the endoplasmic reticulum stress-induced expression of human HRD1 and SEL1 genes:
M. Kaneko, et al.; FEBS Lett.
581
, 5355 (2007)
Abstract
Further Categories Containing This Product:
Tumor Suppressors / Related Products
•
Monoclonal Antibodies
ALX-804-645
Revised 05-Mar-07
Monoclonal Antibody to TRIP-Br1 (TB1-27/2)
SYNONYMS
anti-SERTA Domain-containing Protein 1 MAb (TB1-27/2)
anti-SEI-1 MAb (TB1-27/2)
anti-CDK4-binding Protein p34SEI1 MAb (TB1-27/2)
anti-Transcriptional Regulator Interacting with the PHD-bromodomain 1 MAb (TB1-27/2)
PRODUCT LINE
Cell Cycle
PRODUCT CATEGORY
CDKs & Cyclins Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-645-C050
50 µg
240.00 USD
ALX-804-645-C100
100 µg
360.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Pig
Monkey
CLONE:
TB1-27/2
ISOTYPE:
Mouse IgG2a.
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 27-42 (D
27
PGHAAVAQAPPAVAS
42
) of human TRIP-Br1 [SEI-1].
SPECIFICITY:
Recognizes human, mouse, pig and monkey TRIP-Br1.
APPLICATION:
Immunohistochemistry (1:200)
Immunoprecipitation
Western Blot (1:1’000-1:5’000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Exploiting the TRIP-Br family of cell cycle regulatory proteins as chemotherapeutic drug targets in human cancer:
Z.J. Zang, et al.;
(ahead of print)
, (2007)
General Information
TRIP-Br1 [SEI-1] functions as physiological integrator of transcriptional regulatory signals at E2F-responsive promoters to ensure proper execution of E2F-dependent cell cycle progression. TRIP-Br1 was recently defined as an oncogene.
General Literature References
RBT1, a novel transcriptional co-activator, binds the second subunit of replication protein A:
J.M. Cho, et al.; Nucl. Acids Res.
28
, 3478 (2000)
Abstract
;
Full Text
TRIP-Br: a novel family of PHD zinc finger- and bromodomain-interacting proteins that regulate the transcriptional activity of E2F-1/DP-1:
S.I. Hsu, et al.; EMBO J.
20
, 2273 (2001)
Abstract
;
Full Text
The Drosophila gene taranis encodes a novel trithorax group member potentially linked to the cell cycle regulatory apparatus:
S. Calgaro, et al.; Genetics
160
, 547 (2002)
Abstract
;
Full Text
Regulation of CREB-mediated transcription by association of CDK4 binding protein p34SEI-1 with CBP:
T. Hirose, et al.; Int. J. Mol. Med.
11
, 705 (2003)
Abstract
TRIP-Br links E2F to novel functions in the regulation of cyclin E expression during cell cycle progression and in the maintenance of genomic stability:
K.G. Sim, et al.; Cell Cycle
3
, 1296 (2004)
Abstract
The nuclear protein p34SEI-1 regulates the kinase activity of cyclin-dependent kinase 4 in a concentration-dependent manner:
J. Li, et al.; Biochemistry
43
, 4394 (2004)
Abstract
Expression of a human SOCS protein, HSOCP-1, in peripheral blood eosinophils from patients with atopic dermatitis:
K. Ogawa, et al.; Int. Arch. Allergy Immunol.
134 Suppl 1
, 2 (2004)
Abstract
Dissection of CDK4-binding and transactivation activities of p34(SEI-1) and comparison between functions of p34(SEI-1) and p16(INK4A):
J. Li, et al.; Biochemistry
44
, 13246 (2005)
Abstract
SEI family of nuclear factors regulates p53-dependent transcriptional activation:
R. Watanabe-Fukunaga, et al.; Genes Cells
10
, 851 (2005)
Abstract
;
Full Text
CDCA4 is an E2F transcription factor family-induced nuclear factor that regulates E2F-dependent transcriptional activation and cell proliferation:
R. Hayashi, et al.; J. Biol. Chem.
281
, 35633 (2006)
Abstract
;
Full Text
Further Categories Containing This Product:
Tumor Suppressors / Related Products
•
Monoclonal Antibodies
•
Transcription Regulators Other Products
ALX-210-910
Revised 29-Feb-08
Polyclonal Antibody to CYLD (human) (AF888)
SYNONYMS
anti-Cylindromatosis Tumor Suppressor Protein (human) PAb (AF888)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
NF-kB Pathway Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-910-C050
50 µg
195.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
0.45mg/ml
PURITY DETAIL:
Epitope-affinity purified.
FORMULATION:
Liquid. In PBS containing 1% BSA and 0.02% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 56-70 (G
56
HSRIPSAKGKKNQI
70
) of human CYLD (cylindromatosis tumor suppressor protein).
SPECIFICITY:
Recognizes human CYLD. Detects a band of ~120kDa by Western blot.
APPLICATION:
Immunocytochemistry (on transfected cells; 1:200)
Western Blot (on transfected cells; 1:3'000).
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
