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ALX-270-275
Revised 11-Sep-08
Alsterpaullone
SYNONYMS
9-Nitro-7,12-dihydroindolo-[3,2-
d
][1]benzazepin-6(5
H
)-one
PRODUCT LINE
Cell Cycle
PRODUCT CATEGORY
CDK & Cyclin Inhibitors
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-270-275-M001
1 mg
60.00 USD
ALX-270-275-M005
5 mg
270.00 USD
Product Specification
FORMULA:
C
16
H
11
N
3
O
3
MW:
293.3
CAS NUMBER:
237430-03-4
PURITY:
≥95% (HPLC)
APPEARANCE:
Yellow to brown powder.
SOLUBILITY:
Soluble in DMSO; insoluble in water or 100% ethanol.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
IDENTITY:
Identity determined by IR,
1
H-NMR and
13
C-NMR.
Product Description
Potent inhibitor of CDK1/cyclin B (IC
50
=35nM). Potent and selective inhibitor of CDK2/cyclin A, CDK2/cyclin E (IC
50
=200nM), CDK5/p25 (IC
50
=40nM), CDK5/p35 (IC
50
=40nM) and GSK-3β . Induces apoptosis by activation of caspase-8 and caspase-9 followed by disruption of the mitochondrial potential.
Licensed from NCI.
Product Specific Literature References
ATP-site directed inhibitors of cyclin-dependent kinases
:
N. Gray, et al.; Curr. Med. Chem.
6
, 859 (1999)
Abstract
Fused azepinones with antitumor activity:
C. Kunick; Curr. Pharm. Des.
5
, 181 (1999), (Review)
Abstract
Paullones, a series of cyclin-dependent kinase inhibitors: synthesis, evaluation of CDK1/cyclin B inhibition, and in vitro antitumor activity:
C. Schultz, et al.; J. Med. Chem.
42
, 2909 (1999)
Abstract
Discovery and initial characterization of the paullones, a novel class of small-molecule inhibitors of cyclin-dependent kinases
:
D.W. Zaharevitz, et al.; Cancer Res.
59
, 2566 (1999)
Abstract
2-Substituted paullones: CDK1/cyclin B-inhibiting property and in vitro antiproliferative activity
:
C. Kunick, et al.; Bioorg. Med. Chem. Lett.
10
, 567 (2000)
Abstract
Paullones are potent inhibitors of glycogen synthase kinase-3beta and cyclin-dependent kinase 5/p25
:
M. Leost, et al.; Eur. J. Biochem.
267
, 5983 (2000)
Abstract
Inhibition of CDKs as a therapeutic modality
:
E.A. Sausville, et al.; Ann. NY Acad. Sci.
910
, 207 (2000)
Abstract
A novel, extraneuronal role for cyclin-dependent protein kinase 5 (CDK5): modulation of cAMP-induced apoptosis in rat leukemia cells
:
T. Sandal, et al.; J. Biol. Chem.
277
, 20783 (2002)
Abstract
;
Full Text
Intracellular Targets of Paullones. Identification following affinity purification on immobilized inhibitor
:
M. Knockaert, et al.; J. Biol. Chem.
277
, 25493 (2002)
Abstract
;
Full Text
Alsterpaullone, a novel cyclin-dependent kinase inhibitor, induces apoptosis by activation of caspase-9 due to perturbation in mitochondrial membrane potential
:
T. Lahusen, et al.; Mol Carcinog
36
, 183 (2003)
Abstract
The specificities of protein kinase inhibitors: an update
:
J. Bain, et al.; Biochem. J.
371
, 199 (2003)
Abstract
Further Categories Containing This Product:
Apoptosis Inducers & Inhibitors Other Products
•
GSK-3 Inhibitors
ALX-270-044
Revised 20-Oct-08
3-Aminobenzamide
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP Inhibitors
Ordering Information
Product Numbers:
Format:
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ALX-270-044-G001
1 g
50.00 USD
ALX-270-044-G005
5 g
190.00 USD
Product Specification
FORMULA:
C
7
H
8
N
2
O
MW:
136.2
CAS NUMBER:
3544-24-9
RTECS:
CU8992000
PURITY:
≥97%
APPEARANCE:
White to tan powder.
SOLUBILITY:
Soluble in DMSO or 100% ethanol.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+20°C
HAZARD:
IRRITANT. MAY BE MUTAGENIC.
Product Description
Inhibitor of poly(ADP-ribose) polymerase-1 (PARP-1). Has minimal effect on bacterial toxin-mediated ADP-ribosylation. Apoptosis inhibitor.
Product Specific Literature References
Signal transduction in Coprinus congregatus: evidence for the involvement of G proteins in blue light photomorphogenesis:
K.R. Kozak and I.A. Ross; BBRC
179
, 1225 (1991)
Abstract
Cell death protection by 3-aminobenzamide and other poly(ADP- ribose)polymerase inhibitors: different effects on human natural killer and lymphokine activated killer cell activities:
D. Monti, et al.; BBRC
199
, 525 (1994)
Abstract
Cell death protection by 3-aminobenzamide: impairment of cytoskeleton function in human NK cell-mediated killing:
W. Malorni, et al.; BBRC
199
, 1250 (1994)
Abstract
3-Aminobenzamide protects cells from UV-B-induced apoptosis by acting on cytoskeleton and substrate adhesion:
W. Malorni, et al.; BBRC
207
, 715 (1995)
Abstract
Inactivation of the poly(ADP-ribose) polymerase gene affects oxygen radical and nitric oxide toxicity in islet cells:
B. Heller, et al.; J. Biol. Chem.
270
, 11176 (1995)
Abstract
Inhibitors of poly(ADP-ribose) polymerase block nitric oxide-induced apoptosis but not differentiation in human leukemia HL-60 cells:
M.L. Kuo, et al.; BBRC
219
, 502 (1996)
Abstract
Substrate-assisted catalysis by PARP10 limits its activity to mono-ADP-ribosylation:
H. Kleine, et al.; Mol. Cell
32
, 57 (2008)
Abstract
Further Categories Containing This Product:
Apoptosis Inducers & Inhibitors Other Products
•
DNA Repair Other Products
ALX-340-029
Revised 08-Jul-08
Anandamide
SYNONYMS
AEA
N-Arachidonoylethanolamine
(all-Z)-N-(2-Hydroxyethyl)-5,8,11,14-eicosatetraenamide
PRODUCT LINE
Neurobiology
PRODUCT CATEGORY
Anandamide & Anandamide Analogs
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ALX-340-029-M005
5 mg
51.00 USD
Product Specification
FORMULA:
C
22
H
37
NO
2
MW:
347.5
CAS NUMBER:
94421-68-8
MERCK INDEX:
14:
624
RTECS:
JX3842500
CONCENTRATION:
50mg/ml
PURITY:
≥98%
FORMULATION:
Liquid. Solution in ethanol.
SOLUBILITY:
10mg/ml soluble in DMSO or dimethyl formamide.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 6 months in other organic solvents when stored at -20°C.
HANDLING:
Protect from light and oxygen.
Product Description
Endogenous [1] ligand for the CB
1
receptor (CB
1
: K
i
=52nm; CB
2
: K
i
=1930nm [2]) and TRPV1 (K
i
=5.78µM [3,4]). Inhibits NF-κB activation through direct binding to IKKβ [5] and induces apoptosis independently of cannabinoid or vanilloid receptors [6]. Activates the MAP kinase (MAPK/ERK) signalling pathway [7].
Product Specific Literature References
[1]
Isolation and structure of a brain constituent that binds to the cannabinoid receptor:
W.A. Devane, et al.; Science
258
, 1946 (1992)
Abstract
[2]
Novel analogues of arachidonylethanolamide (anandamide): affinities for the CB1 and CB2 cannabinoid receptors and metabolic stability
:
S. Lin, et al.; J. Med. Chem.
41
, 5353 (1998)
Abstract
[3]
Vanilloid receptors on sensory nerves mediate the vasodilator action of anandamide
:
P.M. Zygmunt, et al.; Nature
400
, 452 (1999)
Abstract
[4]
Structure-activity relationship for the endogenous cannabinoid, anandamide, and certain of its analogues at vanilloid receptors in transfected cells and vas deferens
:
R.A. Ross, et al.; Br. J. Pharmacol.
132
, 631 (2001)
Abstract
[5]
Anandamide inhibits nuclear factor-kappaB activation through a cannabinoid receptor-independent pathway
:
R. Sancho, et al.; Mol. Pharmacol.
63
, 429 (2003)
Abstract
[6]
Anandamide induces cell death independently of cannabinoid receptors or vanilloid receptor 1: possible involvement of lipid rafts
:
K.P. Sarker, et al.; Cell Mol. Life Sci.
60
, 1200 (2003)
Abstract
[7]
The MAP kinase signal transduction pathway is activated by the endogenous cannabinoid anandamide
:
M. Wartmann, et al.; FEBS Lett.
359
, 133 (1995)
Abstract
Biochemistry and pharmacology of arachidonylethanolamide, a putative endogenous cannabinoid:
C.J. Hillard & W.B. Campbell; J. Lipid Res.
38
, 2383 (1997), (Review)
Abstract
The endogenous cannabinoid anandamide inhibits human breast cancer cell proliferation
:
L. De Petrocellis, et al.; PNAS
95
, 8375 (1998)
Abstract
Cannabinoid receptors and their endogenous agonist, anandamide:
J. Axelrod & C.C. Felder; Neurochem. Res.
23
, 575 (1998), (Review)
Abstract
Endocannabinoids: endogenous cannabinoid receptor ligands with neuromodulatory action:
V. Di Marzo, et al.; TINS
21
, 521 (1998), (Review)
Abstract
Endocannabinoids: a new class of vasoactive substances:
M.D. Randall & D.A. Kendall; TIPS
19
, 55 (1998), (Review)
Abstract
Metabolism of anandamide and 2-arachidonoylglycerol: an historical overview and some recent developments:
V. Di Marzo, et al.; Lipids
34
, S319 (1999), (Review)
Abstract
Anandamide: a candidate neurotransmitter heads for the big leagues:
D.W. Self; Nat. Neurosci.
2
, 303 (1999), (Review)
Abstract
New perspectives on enigmatic vanilloid receptors
:
A. Szallasi & V. Di Marzo; TINS
23
, 491 (2000), (Review)
Abstract
Anandamide: some like it hot
:
V. Di Marzo, et al.; TIPS
22
, 346 (2001), (Review)
Abstract
Anandamide is an endogenous inhibitor for the migration of tumor cells and T lymphocytes:
J. Joseph, et al.; Cancer Immunol. Immunother.
53
, 723 (2004)
Abstract
Anandamide-induced cell death in primary neuronal cultures: role of calpain and caspase pathways:
V.A. Movsesyan, et al.; Cell Death Differ.
11
, 1121 (2004)
Abstract
Arachidonylethanolamide induces apoptosis of human glioma cells through vanilloid receptor-1:
E. Contassot, et al.; J. Neuropathol. Exp. Neurol.
63
, 956 (2004)
Abstract
Preferred conformations of endogenous cannabinoid ligand anandamide:
J.Z. Chen, et al.; Life Sci.
76
, 2053 (2005)
Abstract
The cardiovascular actions of anandamide: more targets?:
M.D. Randall; Br. J. Pharmacol.
145
, 565 (2005)
Abstract
;
Full Text
Accumulation of anandamide: Evidence for cellular diversity:
C.J. Hillard and A. Jarrahian; Neuropharmacology
48
, 1072 (2005)
Abstract
Anandamide as an intracellular messenger regulating ion channel activity:
M. van der Stelt and V. Di Marzo; Prostaglandins Other Lipid Mediat.
77
, 111 (2005)
Abstract
Anandamide reduces infarct size in rat isolated hearts subjected to ischaemia-reperfusion by a novel cannabinoid mechanism:
N.J. Underdown, et al.; Br. J. Pharmacol.
146
, 809 (2005)
Abstract
Anandamide inhibits adhesion and migration of breast cancer cells:
C. Grimaldi, et al.; Exp. Cell Res.
312
, 363 (2006)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
To change the solvent, evaporate the ethanol under a gentle stream of nitrogen and immediately add the solvent of choice.
Further Categories Containing This Product:
Endocannabinoids
•
MAPK Pathway Activators
•
Apoptosis Inducers & Inhibitors Other Products
•
Endovanilloids
•
NF-kB Pathway Inhibitors
ALX-420-035
Revised 03-May-05
Apoptosis Activator 2
SYNONYMS
1-[(3,4-Dichlorophenyl)methyl]-1
H
-indole-2,3-dione
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Apoptosis Inducers & Inhibitors Other Products
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ALX-420-035-M005
5 mg
45.00 USD
ALX-420-035-M025
25 mg
180.00 USD
Product Specification
FORMULA:
C
15
H
9
NO
2
Cl
2
MW:
306.1
CAS NUMBER:
79183-19-0
PURITY:
≥98% (NMR)
APPEARANCE:
Orange to red solid.
SOLUBILITY:
Soluble in DMSO or 100% ethanol.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
Product Description
Induces apoptosis by promoting the oligomerization of Apaf-1 into the mature, functional apoptosome, triggering caspase-9 activation resulting in activated caspase-3. Shows strong selectivity for cancer cells against normal cell lines. Acts in a similar way like apoptosis inducer PETCM (Prod. No.
ALX-420-031
).
Product Specific Literature References
Direct activation of the apoptosis machinery as a mechanism to target cancer cells:
J.T. Nguyen and J.A. Wells; PNAS
100
, 7533 (2003)
Abstract
;
Full Text
Further Categories Containing This Product:
Caspases Other Products
ALX-850-235
Revised 29-May-08
Apoptosis Inducer Set
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Apoptosis Inducers & Inhibitors Other Products
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ALX-850-235-KI01
1 Set
315.00 USD
Product Specification
KIT/SET CONTAINS:
Contains 5
ready-to-use
reagents:
- Actinomycin D, 50µl (10mM), violet cap
- Camptothecin, 1ml (2mM), red cap
- Cycloheximide, 1ml (100mM), green cap
- Dexamethasone, 1ml (10mM), blue cap
- Etoposide, 100µl (100mM), yellow cap
FORMULATION:
Liquid. In DMSO
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
Product Description
The set contains 5
ready-to-use
reagents that induce apoptosis through different mechanisms. Actinomycin D, an antineoplastic antibiotic, inhibits RNA synthesis. Camptothecin, an inhibitor of nuclear topoisomerase, induces apoptosis in many types of cells. Cycloheximide, an active antibiotic against many yeast and fungi, inhibits protein synthesis. Dexamethasone, an active and highly stable glucocorticoid, probably induces apoptosis by binding and activating the intracellular glucocorticoid receptor. Etoposide, a derivative of podophyllotoxin, inhibits topoisomerase activity.
Product Specific Literature References
Topoisomerase inhibitors induce apoptosis in thymocytes:
Y. Onishi, et al.; Biochim Biophys Acta
1175
, 147 (1993)
Abstract
Dexamethasone and etoposide induce apoptosis in rat thymocytes from different phases of the cell cycle
:
H.O. Fearnhead, et al.; Biochem. Pharmacol.
48
, 1073 (1994)
Abstract
Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl
:
S.J. Martin, et al.; J. Exp. Med.
182
, 1545 (1995)
Abstract
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis
:
D.W. Nicholson, et al.; Nature
376
, 37 (1995)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
We recommend using 1000x dilutions for inducing apoptosis in cell cultures. Optimal doses may vary for different cells and culture conditions and must be determined individually.
MANUFACTURER
Manufactured by BioVision.
Further Categories Containing This Product:
Panels / Sets
ALX-340-042
Revised 07-Jan-06
Arvanil
SYNONYMS
N-[(4-Hydroxy-3-methoxyphenyl)methyl]-5
Z
,8
Z
,11
Z
,14
Z
-eicosatetraenamide
PRODUCT LINE
Neurobiology
PRODUCT CATEGORY
Cannabinoid Receptor Agonists & Antagonists / Related Products
Ordering Information
Product Numbers:
Format:
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Unit Price:
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ALX-340-042-M005
5 mg
25.00 USD
Product Specification
FORMULA:
C
28
H
41
NO
3
MW:
439.6
CAS NUMBER:
128007-31-8
PURITY:
≥98%
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light. Keep cool and dry.
Product Description
"Hybrid" activator of CB
1
receptor (CB
1
: K
i
=0.5µM; CB
2
: K
i
=>15µM) and TRPV1 (K
i
=0.3µM). Also inhibits anandamide uptake (IC
50
=3.6µM) and fatty acid amide hydrolase (FAAH) (IC
50
=3µM). Analgesic, vasodilatory and anti-inflammatory
in vivo
. Apoptosis inducer.
Product Specific Literature References
Unsaturated long-chain N-acyl-vanillyl-amides (N-AVAMs): vanilloid receptor ligands that inhibit anandamide-facilitated transport and bind to CB1 cannabinoid receptors:
D. Melck, et al.; BBRC
262
, 275 (1999)
Abstract
Neurobehavioral activity in mice of N-vanillyl-arachidonyl-amide
:
V. Di Marzo, et al.; Eur. J. Pharmacol.
406
, 363 (2000)
Abstract
A structure/activity relationship study on arvanil, an endocannabinoid and vanilloid hybrid
:
V. Di Marzo, et al.; J. Pharmacol. Exp. Ther.
300
, 984 (2002)
Abstract
The CB1/VR1 agonist arvanil induces apoptosis through an FADD/caspase-8-dependent pathway
:
R. Sancho, et al.; Br. J. Pharmacol.
140
, 1035 (2003)
Abstract
Evidence against the presence of an anandamide transporter
:
S.T. Glaser, et al.; PNAS
100
, 4269 (2003)
Abstract
Arvanil, a hybrid endocannabinoid and vanilloid compound, behaves as an antihyperkinetic agent in a rat model of Huntington's disease:
E. de Lago, et al.; Brain Res.
1050
, 210 (2005)
Abstract
Arvanil inhibits T lymphocyte activation and ameliorates autoimmune encephalomyelitis:
A.M. Malfitano, et al.; J. Neuroimmunol.
171
, 110 (2006)
Abstract
Further Categories Containing This Product:
Anandamide Uptake Inhibitors
•
Apoptosis Inducers & Inhibitors Other Products
•
TRPV1 Agonists and Antagonists / Related Products
•
Analgesic / Anti-nociceptive Agents / Related Products
•
Anti-inflammatory Agents Other Products
•
Fatty Acid Amide Hydrolase [FAAH] / Related Products
ALX-270-201
Revised 18-Sep-07
Aurintricarboxylic acid
SYNONYMS
ATA
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Topoisomerase Inhibitors Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
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ALX-270-201-G001
1 g
15.00 USD
Product Specification
FORMULA:
C
22
H
14
O
9
MW:
422.4
CAS NUMBER:
4431-00-9
PURITY:
~85%
APPEARANCE:
Red solid.
SOLUBILITY:
Soluble in aqueous alkali.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+20°C
HAZARD:
IRRITANT.
Product Description
Potent inhibitor of DNA topoisomerase II
in vitro
and protein-nucleic acid interactions. Stimulates the tyrosine phosphorylation of MAP kinases. Inhibits both major calpain isoforms. Potent and selective inhibitor of SARS coronavirus replication. Apoptosis inhibitor.
Product Specific Literature References
Mechanism of action of polymeric aurintricarboxylic acid, a potent inhibitor of protein--nucleic acid interactions:
R.G. Gonzalez, et al.; Biochemistry
19
, 4299 (1980)
Abstract
Delayed application of aurintricarboxylic acid reduces glutamate- induced cortical neuronal injury:
C.A. Csernansky, et al.; J. Neurosci. Res.
38
, 101 (1994)
Abstract
Inhibition of topoisomerase II by aurintricarboxylic acid: implications for mechanisms of apoptosis:
D.R. Catchpoole & B.W. Stewart; Anticancer Res.
14
, 853 (1994)
Abstract
Aurintricarboxylic acid, a putative inhibitor of apoptosis, is a potent inhibitor of DNA topoisomerase II in vitro and in Chinese hamster fibrosarcoma cells:
Y. Benchokroun, et al.; Biochem. Pharmacol.
49
, 305 (1995)
Abstract
A neuroprotective compound, aurin tricarboxylic acid, stimulates the tyrosine phosphorylation cascade in PC12 cells:
N. Okada & S. Koizumi; J. Biol. Chem.
270
, 16464 (1995)
Abstract
;
Full Text
Potent and selective inhibition of SARS coronavirus replication by aurintricarboxylic acid
:
R. He, et al.; BBRC
320
, 1199 (2004)
Abstract
Further Categories Containing This Product:
Apoptosis Inducers & Inhibitors Other Products
ALX-450-014
Revised 22-Feb-08
BAPTA/AM
SYNONYMS
1,2-
bis
(2-Aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
tetrakis
(acetoxymethyl) ester
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calcium Chelators / Caged Calcium Chelators
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-450-014-M010
10 mg
48.00 USD
ALX-450-014-M050
50 mg
195.00 USD
Product Specification
FORMULA:
C
34
H
40
N
2
O
18
MW:
764.7
CAS NUMBER:
126150-97-8
PURITY:
≥98% (HPLC)
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in DMSO (10mg/ml).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Packaged under inert gas. Protect from light and moisture.
HAZARD:
IRRITANT.
IDENTITY:
Determined by
1
H-NMR.
Product Description
Cell permeable acetoxymethyl ester derivative of BAPTA (Prod. No.
ALX-450-013
). Useful for controlling intracellular Ca
2+
concentration. Induces inactivation of protein kinase C. Also inhibits thapsigargin-induced apoptosis in rat thymocytes.
Product Specific Literature References
Physiological [Ca2+]i level and pump-leak turnover in intact red cells measured using an incorporated Ca chelator:
V.L. Lew, et al.; Nature
298
, 478 (1982)
Abstract
BAPTA induces a decrease of intracellular free calcium and a translocation and inactivation of protein kinase C in macrophages:
P. Dieter, et al.; Biol. Chem. Hoppe-Seyler
374
, 171 (1993)
Abstract
Intracellular calcium chelator, BAPTA-AM, prevents cocaine-induced ventricular fibrillation:
G.E. Billman; Am. J. Physiol.
265
, H1529 (1993)
Abstract
Intracellular Ca2+ signals activate apoptosis in thymocytes: studies using the Ca(2+)-ATPase inhibitor thapsigargin:
S. Jiang, et al.; Exp. Cell. Res.
212
, 84 (1994)
Abstract
Stimulation of the ERK pathway by GTP-loaded Rap1 requires the concomitant activation of Ras, protein kinase C, and protein kinase A in neuronal cells:
T. Bouschet, et al.; J. Biol. Chem.
278
, 4778 (2003)
Abstract
;
Full Text
Further Categories Containing This Product:
PKC Inhibitors
•
Apoptosis Inducers & Inhibitors Other Products
•
Nuclear Envelope & Nuclear Transport
ALX-620-051
Revised 30-Jun-08
Bisbenzimide H33258 . trihydrochloride
SYNONYMS
HOE 33258
2'-4-(Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-
bis
-1
H
-benzimidazole . 3HCl
PRODUCT LINE
Other Products
PRODUCT CATEGORY
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
Ordering Information
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Format:
Size:
Unit Price:
Quantity:
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ALX-620-051-M010
10 mg
22.00 USD
ALX-620-051-M050
50 mg
35.00 USD
Product Specification
FORMULA:
C
25
H
24
N
6
O . 3HCl
MW:
424.4 . 109.4
CAS NUMBER:
23491-45-4
RTECS:
SM1140500
PURITY:
≥98% (TLC)
APPEARANCE:
Yellow to greenish powder.
SOLUBILITY:
Soluble in water (10mg/ml).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light.
HAZARD:
MAY BE MUTAGENIC. HARMFUL.
IDENTITY:
Identity determined by IR and
1
H-NMR.
Product Description
Cell permeable fluorescent dye with low cytotoxicity. Useful for staining DNA, chromosomes and nuclei [1,2]. Can be used with chromomycin A
3
(Prod. No.
ALX-380-055
) to distinguish chromosomes by their total DNA concentration and their DNA base composition [3,4]. Binds to A:T pairs in DNA. Also useful for controlling gene expression through binding to RNA aptamers inserted into 5' untranslated region of a messenger RNA [5]. Increases the rate of channel-mediated Ca
2+
efflux from junctional cytoplasmic reticulum vesicles [6]. Ex(max): 346nm; Em(max): 460nm.
Product Specific Literature References
[1]
Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis
:
S.A. Latt & G. Stetten; J. Histochem. Cytochem.
24
, 24 (1976)
Abstract
[2]
Comparison of cellular and nuclear flow cytometric techniques for discriminating apoptotic subpopulations:
K.H. Elstein & R.M. Zucker; Exp. Cell. Res.
211
, 322 (1994)
Abstract
[3]
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MDR Other Products
ALX-620-050
Revised 02-Sep-05
Bisbenzimide H33342 . trihydrochloride
SYNONYMS
HOE 33342
2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-
bis
-1
H
-benzimidazole . 3HCl
PRODUCT LINE
Other Products
PRODUCT CATEGORY
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
Ordering Information
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Format:
Size:
Unit Price:
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ALX-620-050-M010
10 mg
22.00 USD
ALX-620-050-M050
50 mg
35.00 USD
