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Caspase Recruitment Domain [CARD] Proteins / Related Products
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ALX-210-017 Revised 12-Apr-07
Polyclonal Antibody to NALP1 (human) (CT) (Bur 242)
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SYNONYMS anti-NACHT-, LRR- and PYD-containing Protein 1 (human) (CT) PAb (Bur 242)
anti-DEFCAP (human) (CT) PAb (Bur 242)
anti-NAC (human) (CT) PAb (Bur 242)
anti-CARD7 (human) (CT) PAb (Bur 242)
anti-Caspase Recruitment Domain-containing Protein 7 (human) (CT) PAb (Bur 242)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Inflammasome
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ALX-210-017-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Antiserum containing PBS and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1058-1077 (C1058VPSPASQGDLHTKPLGTDD1077) of C-terminal human NALP1 (NACHT-, LRR- and PYD-containing protein 1).
SPECIFICITY: Recognizes human NALP1. Does not cross-react with mouse NALP1.
APPLICATION: Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-603.
Product Specific Literature References
A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis: Z.L. Chu, et al.; J. Biol. Chem. 276, 9239 (2001) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • Polyclonal Antibodies
 
 
ALX-210-018 Revised 12-Apr-07
Polyclonal Antibody to NALP1 (human) (NT) (Bur 241)
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SYNONYMS anti-NACHT-, LRR- and PYD-containing Protein 1 (human) (NT) PAb (Bur 241)
anti-DEFCAP (human) (NT) PAb (Bur 241)
anti-NAC (human) (NT) PAb (Bur 241)
anti-CARD7 (human) (NT) PAb (Bur 241)
anti-Caspase Recruitment Domain-containing Protein 7 (human) (NT) PAb (Bur 241)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Inflammasome
Ordering Information
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ALX-210-018-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Antiserum containing PBS and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 161-180 (P161SSPDHESPSQESPNAPTST180) of N-terminal human NALP1 (NACHT-, LRR- and PYD-containing protein 1).
SPECIFICITY: Recognizes human NALP1. Does not cross-react with mouse NALP1.
APPLICATION: Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-603.
Product Specific Literature References
A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis: Z.L. Chu, et al.; J. Biol. Chem. 276, 9239 (2001) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • Polyclonal Antibodies
 
 
ALX-210-352 Revised 30-Oct-06
Polyclonal Antibody to MDA5 (mouse) (AL180)
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SYNONYMS anti-Melanoma Differentiation-associated Gene 5 (mouse) PAb (AL180)
anti-Helicard (mouse) PAb (AL180)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
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ALX-210-352-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Contains 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse MDA5 (melanoma differentiation-associated gene 5) (aa 2-208).
SPECIFICITY: Recognizes mouse MDA5.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Overexpression of Helicard, a CARD-Containing Helicase Cleaved during Apoptosis, Accelerates DNA Degradation: M. Kovacsovics, et al.; Curr. Biol. 12, 838 (2002) Abstract
General Information
MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract
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Further Categories Containing This Product:
NF-kB Pathway Other Products • Antiviral Signalling / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • Polyclonal Antibodies
 
 
ALX-210-373 Revised 02-Apr-07
Polyclonal Antibody to Nod2 (human)
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SYNONYMS anti-CARD15 (human) PAb
anti-Caspase Recruitment Domain-containing Protein 15 (human) PAb
anti-Ibd1 (human) PAb
anti-Inflammatory Bowel Disease Protein 1 (human) PAb
PRODUCT LINE Inflammation
PRODUCT CATEGORY Nod-complex / Related Products
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ALX-210-373-1   1 Vial 330.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute with 100µl water containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human Nod2 (CARD15) (aa 28-301).
SPECIFICITY: Recognizes human Nod2.
APPLICATION: Immunoprecipitation*
Western Blot (1:500 to 1:1'000)*
*) tested with cells overexpressing Nod2. In monocytes, Nod2 is expressed at low levels and may not be detected with this antibody.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 2 years after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution store at +4°C.
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • Polyclonal Antibodies
 
 
ALX-210-903 Revised 25-Jan-08
Polyclonal Antibody to CARMA1 (AL220)
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SYNONYMS anti-CARD-containing MAGUK Protein 1 PAb (AL220)
anti-CARD11 PAb (AL220)
anti-Caspase Recruitment Domain-containnig Protein 11 PAb (AL220)
anti-Bimp3 PAb (AL220)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Pathway Other Products
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ALX-210-903-R100   100 µl 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum.
IMMUNOGEN: N-terminal human CARMA1 (CARD-containing MAGUK protein 1) (aa 1-221 containing the CARD domain).
SPECIFICITY: Recognizes human and mouse CARMA1. Detects a band of ~130Dka by Western blot.
APPLICATION: Immunocytochemistry
Immunohistochemistry
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-606.
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Product Specific Literature References
CARMA1 is a critical lipid raft-associated regulator of TCR-induced NF-kappaB activation: O. Gaide, et al.; Nat. Immunol. 3, 836 (2002) Abstract
Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1: R.B. Medeiros, et al.; Science 316, 754 (2007) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Carma1, a CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappaB activation: O. Gaide, et al.; FEBS Lett. 496, 121 (2001) Abstract
A requirement for CARMA1 in TCR-induced NF-kappa B activation: D. Wang, et al.; Nat. Immunol. 3, 830 (2002) Abstract
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • Polyclonal Antibodies
 
 
ALX-210-904 Revised 25-Jan-08
Polyclonal Antibody to NALP1 (human) (AL176)
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SYNONYMS anti-NACHT-, LRR- and PYD-containing Protein 1 (human) PAb (AL176)
anti-DEFCAP (human) PAb (AL176)
anti-NAC (human) PAb (AL176)
anti-CARD7 (human) PAb (AL176)
anti-Caspase Recruitment Domain-containing Protein 7 (human) PAb (AL176)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Inflammasome
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ALX-210-904-R100   100 µl 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
PURITY: Affinity purified.
FORMULATION: Liquid. Neat serum.
IMMUNOGEN: Synthetic peptide corresponding to aa 2-25 (A2GGAWGRLACYLEFLKKEELKEFQ25) of N-terminal human NALP1 (NACHT-, LRR- and PYD-containing protein 1).
SPECIFICITY: Recognizes human NALP1. Does not cross-react with mouse NALP1.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-603.
Product Images
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Product Specific Literature References
The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta: F. Martinon; Mol. Cell. 10, 417 (2002) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Signalling: Initiation of pro-IL-1beta processing: E. Bell; Nat. Rev. Immunol. 2, 719 (2002), (Review) Abstract
The PYRIN-CARD protein ASC is an activating adaptor for caspase-1: S.M. Srinivasula, et al.; J. Biol. Chem. 277, 21119 (2002) Abstract
NALPS: a novel protein family involved in inflammation: J. Tschopp, et al.; Nat. Rev. Mol. Cell Biol. 4, 95 (2003), (Review) Abstract; Full Text
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • Polyclonal Antibodies
 
 
ALX-210-905 Revised 23-May-08
Polyclonal Antibody to Asc (AL177)
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SYNONYMS anti-Pycard PAb (AL177)
anti-Apoptosis-associated Speck-like Protein Containing CARD PAb (AL177)
anti-TMS1 PAb (AL177)
anti-Target of Methylation-induced Silencing 1 PAb (AL177)
anti-CARD5 PAb (AL177)
anti-Caspase Recruitment Domain-containing Protein 5 PAb (AL177)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Inflammasome
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ALX-210-905-R100   100 µl 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 2-27 (G2RARDAILDALENLTAEELKKFKLKL27) of N-terminal human Asc (apoptosis-associated speck-like protein containing CARD; Pycard).
SPECIFICITY: Recognizes human and mouse Asc.
APPLICATION: Immunocytochemistry
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-602.
Product Images
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Product Specific Literature References
The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta: F. Martinon, et al.; Mol. Cell. 10, 417 (2002) Abstract
NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder: L. Agostini, et al.; Immunity 20, 319 (2004) Abstract
P2X7 Receptor Differentially Couples to Distinct Release Pathways for IL-1{beta} in Mouse Macrophage: P. Pelegrin, et al.; J. Immunol. 180, 7147 (2008) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Signalling: Initiation of pro-IL-1beta processing: E. Bell; Nat. Rev. Immunol. 2, 719 (2002) Abstract
The PYRIN-CARD protein ASC is an activating adaptor for caspase-1: S.M. Srinivasula, et al.; J. Biol. Chem. 277, 21119 (2002) Abstract
NALPS: a novel protein family involved in inflammation: J. Tschopp, et al.; Nat. Rev. Mol. Cell Biol. 4, 95 (2003), (Review) Abstract; Full Text
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • Polyclonal Antibodies
 
 
ALX-210-929 Revised 16-Apr-08
Polyclonal Antibody to Cardif (human) (AT107)
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SYNONYMS anti-CARD Adapter Inducing Interferon-β (human) PAb (AT107)
anti-IPS-1 (human) PAb (AT107)
anti-Interferon-β Promoter Stimulator Protein 1 (human) PAb (AT107)
anti-MAVS (human) PAb (AT107)
anti-Mitochondrial Antiviral Signalling Protein (human) PAb (AT107)
anti-VISA (human) PAb (AT107)
anti-Virus-induced Signalling Adapter (human) PAb (AT107)
PRODUCT LINE Immunology
PRODUCT CATEGORY Antiviral Signalling / Related Products
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ALX-210-929-C100   100 µg 370.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).
SPECIFICITY: Recognizes human cardif.
APPLICATION: Immunocytochemistry (1:500)
Immunoprecipitation (1:100)
Western Blot (1:2’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Regulation of antiviral responses by a direct and specific interaction between TRAF3 and Cardif : S. K. Saha; EMBO J. 25, 3257 (2006) Abstract
Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008) Abstract
General Information
RIG-I (retinoic acid-inducible gene I; Ddx58) and Mda5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons.
Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and Mda5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005) Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005) Abstract
CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006) Abstract
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • NF-kB Pathway Other Products • Polyclonal Antibodies
 
 
ALX-210-932 Revised 28-Feb-07
Polyclonal Antibody to RIG-I (human) (AT111)
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SYNONYMS anti-RIG-1 (human) PAb (AT111)
anti-Retinoic Acid-inducible Gene 1 Protein (human) PAb (AT111)
anti-DEAD-box Protein 58 (human) PAb (AT111)
anti-Probable ATP-dependent RNA Helicase DDX58 (human) PAb (AT111)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
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ALX-210-932-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIG-I (retinoic acid-inducible gene 1 protein) (aa 201-713).
SPECIFICITY: Recognizes human RIG-I.
APPLICATION: Immunoprecipitation (1:100)
Western blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
General Information
RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Virology. Sensing viral RNA amid your own: T. Fujita; Science 314, 935