ALEXIS Biochemicals: URB754

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ALX-215-054

Revised 20-Oct-08

Polyclonal Antibody to Monoacylglycerol Lipase
SYNONYMS	anti-MGL PAb
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Diacylglycerol Lipase [DAGL] & Monoacylglycerol Lipase [MGL] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-215-054-1		1 vial	357.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Others
SOURCE/HOST:	From rabbit
PURITY DETAIL:	Affinity-purified IgG.
FORMULATION:	Liquid. In TBS, pH 7.4, containing 50% glycerol, 0.5 mg/ml BSA and 0.02% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 1-14 (M¹PEESSPRRTPQSI¹⁴) of human monoacylglycerol lipase (MAGL).
SPECIFICITY:	Recognizes human, mouse, rat and bovine MAGL. Detects a band of ~35kDa by Western blot.
APPLICATION:	Immunohistochemistry (formalin-fixed paraffin sections) Western Blot Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles.

Further Categories Containing This Product:

Polyclonal Antibodies

ALX-270-172

Revised 04-Dec-07

RHC-80267
SYNONYMS	U-57908 1,6-bis(Cyclohexyloximinocarbonylamino)hexane
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Diacylglycerol Lipase [DAGL] & Monoacylglycerol Lipase [MGL] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-172-M005		5 mg	40.00 USD
ALX-270-172-M010		10 mg	65.00 USD

Product Specification

FORMULA:	C₂₀H₃₄N₄O₄
MW:	394.5
CAS NUMBER:	83654-05-1
PURITY:	≥98%
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in DMSO or 100% ethanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+20°C

Product Description

Selective inhibitor of diacylglycerol lipase activity. Also inhibits glucose- and carbachol-induced insulin release from intact islets.

Product Specific Literature References

Relative activities of rat and dog platelet phospholipase A2 and diglyceride lipase. Selective inhibition of diglyceride lipase by RHC 80267: C.A. Southerland & D. Amin; J. Biol. Chem. 257, 14006 (1982) Abstract; Full Text
RHC 80267 inhibits thyrotropin-stimulated prostaglandin release from rat thyroid lobes: S. Levasseur, et al.; Prostaglandins 27, 673 (1984) Abstract
Inhibitors of diacylglycerol lipase and diacylglycerol kinase inhibit carbamylcholine-stimulated responses in guinea pig pancreatic minilobules: M. Chandra Sekar & L.E. Hokin; Arch. Biochem. Biophys. 256, 509 (1987) Abstract
Diacylglycerol provides arachidonic acid for lipoxygenase products that mediate angiotensin II-induced aldosterone synthesis: R. Natarajan, et al.; BBRC 156, 717 (1988) Abstract
Arachidonic acid liberated by diacylglycerol lipase is essential for the release mechanism in chromaffin cells from bovine adrenal medulla: B. Rindlisbacher, et al.; J. Neurochem. 54, 1247 (1990) Abstract
Arachidonic acid release from diacylglycerol in human neutrophils. Translocation of diacylglycerol-deacylating enzyme activities from an intracellular pool to plasma membrane upon cell activation: J. Balsinde, et al.; J. Biol. Chem. 266, 15638 (1991) Abstract; Full Text

ALX-550-519

Revised 03-Jul-07

URB602
SYNONYMS	[1,1’-Biphenyl]-3-yl-carbamic acid cyclohexyl ester
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Diacylglycerol Lipase [DAGL] & Monoacylglycerol Lipase [MGL] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-550-519-M005		5 mg	34.00 USD
ALX-550-519-M025		25 mg	150.00 USD

Product Specification

FORMULA:	C₁₉H₂₁NO₂
MW:	295.4
CAS NUMBER:	565460-15-3
PURITY:	≥98%
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in 100% ethanol (10mg/ml), DMSO (30mg/ml) or dimethyl formamide (30mg/ml); also soluble in a 1:1 solution in DMSO:PBS, pH 7.2 (0.25mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Sparingly soluble in aqueous buffers. For maximum solubility dissolve first in DMSO and then dilute with the aqeous buffer of choice. We do not recommend storing the aqueous solution for more than one day.

Product Description

Selective noncompetitive inhibitor of monoacylglycerol lipase (MGL) (IC₅₀=28µM). Does not inhibit FAAH, DAG lipase or COX-2.

Product Specific Literature References

An endocannabinoid mechanism for stress-induced analgesia: A.G. Hohmann, et al.; Nature 435, 1108 (2005) Abstract
Inhibition of fatty-acid amide hydrolase enhances cannabinoid stress-induced analgesia: sites of action in the dorsolateral periaqueductal gray and rostral ventromedial medulla: R.L. Suplita, 2nd, et al.; Neuropharmacology 49, 1201 (2005) Abstract

ALX-550-410

Revised 01-Jun-06

URB754
SYNONYMS	6-Methyl-2-[(4-methylphenyl)amino]-4H-3,1-benzoxazin-4-one
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Diacylglycerol Lipase [DAGL] & Monoacylglycerol Lipase [MGL] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-550-410-M001		1 mg	15.00 USD
ALX-550-410-M005		5 mg	25.00 USD
ALX-550-410-M025		25 mg	100.00 USD

Product Specification

FORMULA:	C₁₆H₁₄N₂O₂
MW:	266.3
CAS NUMBER:	86672-58-4
PURITY:	≥98%
APPEARANCE:	White to off-white crystalline solid.
SOLUBILITY:	Soluble in DMSO and dimethyl formamide. Also soluble in aqueous solutions.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 2 years when stored at -20°C. We do not recommend storing aqueous solutions for more than one day.
HANDLING:	Protect from light.

Product Description

Potent, noncompetitive inhibitor of monoacylglycerol lipase (MGL), which hydrolyzes 2-arachidonoylglycerol (2-AG) to arachidonic acid and glycerol. IC₅₀=200nM for the recombinant rat brain enzyme. Inhibits rat brain fatty acid amide hydrolase (FAAH) less effectively (IC₅₀=32µM) and binds weakly to the rat CB₁ receptor (IC₅₀=3.8µM).

Product Specific Literature References

Selective inhibition of 2-AG hydrolysis enhances endocannabinoid signaling in hippocampus: J.K. Makara, et al.; Nat. Neurosci. 8, 1139 (2005) Abstract