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NF-kB Pathway
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ALX-804-361 Revised 09-May-07
Monoclonal Antibody to NF-κB (p65) (112A1021)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors / Related Products
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ALX-804-361-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 112A1021
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 526-539 of human NF-κB (p65 subunit) .
SPECIFICITY: Recognizes human and mouse NF-κB (p65 subunit ). Detects a band of ~65kDa by Western blot.
APPLICATION: Flow Cyctometry (2.5µg/ml) (see [1])
Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
Product Specific Literature References
[1] Human keratinocytes respond to interleukin-18: implication for the course of chronic inflammatory skin diseases: M. Wittmann, et al.; J. Invest. Dermatol. 124, 1225 (2005) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-849 Revised 16-Aug-07
Monoclonal Antibody to RIG-I (Alme-1)
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SYNONYMS anti-RIG-1 MAb (Alme-1)
anti-Retinoic Acid-inducible Gene 1 Protein MAb (Alme-1)
anti-DEAD-box Protein 58 MAb (Alme-1)
anti-Probable ATP-dependent RNA Helicase DDX58 MAb (Alme-1)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
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ALX-804-849-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Alme-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIG-I (retinoic acid-inducible gene 1 protein) (aa 201-713).
SPECIFICITY: Recognizes human and mouse RIG-I.
APPLICATION: Immunohistochemistry (paraffin sections)
Immunoprecipitation (1:100)
Western blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Virology. Sensing viral RNA amid your own: T. Fujita; Science 314, 935 (2006) Abstract
5’-Triphosphate RNA is the ligand for RIG-I: V. Hornung, et al.; Science 314, 994 (2006) Abstract
RIG-I-mediated antiviral responses to single-stranded RNA bearing 5’-phosphates: A. Pichlmair, et al.; Science 314, 997 (2006) Abstract
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Further Categories Containing This Product:
Antiviral Signalling / Related Products • NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • Polyclonal Antibodies
 
 
ALX-804-139 Revised 09-May-07
Monoclonal Antibody to RIP2 (human) (Nick-1)
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SYNONYMS anti-Receptor-interacting Protein 2 (human) MAb (Nick-1)
anti-CARDIAK (human) MAb (Nick-1)
anti-CARD-containing Interleukin-1β Converting Enzyme Associated Kinase (human) MAb (Nick-1)
anti-RICK (human) MAb (Nick-1)
anti-RIPK2 (human) MAb (Nick-1)
anti-Receptor-interacting Serine/Threonine Protein Kinase 2 (human) MAb (Nick-1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY RIP Kinases
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ALX-804-139-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Nick-1
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIP2 (receptor-interacting protein 2) (aa 1-322).
SPECIFICITY: Recognizes human RIP2.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-604.
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Product Specific Literature References
Participation of Rip2 in LPS signaling is independent of its kinase activity: C. Lu, et al.; J. Biol. Chem. 280, 16278 (2005) Abstract; Full Text
PIDD mediates NF-kB activation in Response to DNA damage: Janssens, et al.; Cell 123, 1079 (2005) Abstract
Participation of Rip2 in lipopolysaccharide signaling is independent of its kinase activity: C. Lu, et al.; J. Biol. Chem. 280, 16278 (2005) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Nod-complex / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Monoclonal Antibodies
 
 
ALX-804-155 Revised 27-Feb-07
Monoclonal Antibody to RIP3 (human) (Rippy-3)
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SYNONYMS anti-Receptor-interacting Protein 3 (human) MAb (Rippy-3)
anti-Receptor-interacting Serine/Threonine Protein Kinase 3 (human) MAb (Rippy-3)
anti-RIP-like Protein Kinase 3 (human) MAb (Rippy-3)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY RIP Kinases
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ALX-804-155-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Rippy-3
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIP3 (receptor-interacting protein 3).
SPECIFICITY: Recognizes human RIP3.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-608.
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General Information
Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNF-R1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3 [1-3]. RIP3 contains an N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNF-R1, mediates TNF-R1 induced apoptosis, and attenuates RIP and TNF-R1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues [2,3]. Meanwhile RIP4 has been identified and initially characterized. These recent data suggest that RIP4 is involved in NF-κB and JNK signalling and that caspase-dependent processing of RIP4 may negatively regulate NF-κB-dependent pro-survival or pro-inflammatory signals [4].
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB: P.W. Yu, et al.; Curr. Biol. 9, 539 (1999) Abstract
[2] RIP3, a novel apoptosis-inducing kinase: X. Sun, et al.; J. Biol. Chem. 274, 16871 (1999) Abstract; Full Text
[3] Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB: N.J. Pazdernik, et al.; Mol. Cell. Biol. 19, 6500 (1999) Abstract; Full Text
[4] RIP4 (DIK/PKK), a novel member of the RIP kinase family, activates NF-kappa B and is processed during apoptosis: E. Meylan, et al.; EMBO Rep. 3, 1201 (2002) Abstract; Full Text
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Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-372 Revised 22-May-08
Monoclonal Antibody to TANK-binding Kinase 1 (108A429)
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SYNONYMS anti-TBK1 MAb (108A429)
anti-NAK MAb (108A429)
anti-NF-κB-activating Kinase MAb (108A429)
anti-T2K MAb (108A429)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY IkB Kinases [IKKs] / Related Products
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ALX-804-372-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 108A429
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 563-577 (YYQFKKDKAERRLAYC) of human TBK1 (TANK-binding kinase 1).
SPECIFICITY: Recognizes human, mouse, rat, dog and bovine TBK1. Detects a band of ~80kDa by Western blot.
APPLICATION: Western Blot (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
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Product Description
TANK-binding Kinase 1 (TBK1) is a member of the IKK family. TANK (TRAF-associated NF-κB activator) interacts with TBK1 and activates NF-κB in a kinase-dependent manner [1].
Product Specific Literature References
[1] NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase: J.L. Pomerantz & D. Baltimore; EMBO J. 18, 6694 (1999) Abstract; Full Text
[2] IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway: K.A. Fitzgerald, et al.; Nat. Immunol. 4, 491 (2003) Abstract
[3] Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I: R. Sumpter, Jr., et al.; J. Virol. 79, 2689 (2005) Abstract; Full Text
[4] Gene expression and antiviral activity of alpha/beta interferons and interleukin-29 in virus-infected human myeloid dendritic cells: P. Osterlund, et al.; J. Virol. 79, 9608 (2005) Abstract
Further Categories Containing This Product:
Antiviral Signalling / Related Products • Monoclonal Antibodies
 
 
ALX-804-733 Revised 05-Feb-08
Monoclonal Antibody to Ribosomal Protein S3 (human) (RP159-1)
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SYNONYMS anti-RPS3 (human) MAb (RP159-1)
anti-40S Ribosomal Protein S3 (human) MAb (RP159-1)
anti-IMR-90 Ribosomal Protein S3 (human) MAb (RP159-1)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Ribosomal Proteins / Related Products
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ALX-804-733-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: RP159-1
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human ribosomal protein S3.
SPECIFICITY: Recognizes human ribosomal protein S3.
APPLICATION: ELISA (direct and indirect: 1:500-1:5’000)
Western Blot (1:500-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 1-2 weeks when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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Product Specific Literature References
Interaction of Hsp90 with ribosomal proteins protects from ubiquitination and proteasome-dependent degradation: T.S. Kim, et al.; Mol. Biol. Cell 17, 824 (2006) Abstract; Full Text
Reduction of invasion in human fibrosarcoma cells by ribosomal protein S3 in conjunction with Nm23-H1 and ERK: S.H. Kim & J. Kim; Biochim. Biophys. Acta 1763, 823 (2006) Abstract
Ribosomal protein S3: a KH domain subunit in NF-kappaB complexes that mediates selective gene regulation: F. Wan, et al.; Cell 131, 927 (2007) Abstract
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
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Further Categories Containing This Product:
Co-Chaperones & HSP-interacting Proteins / Related Products • NF-kB Pathway Other Products • DNA Endonucleases / Related Products • DNA Endonucleases / Related Products • MAPK Pathway Other Products • Monoclonal Antibodies
 
 
ALX-400-047 Revised 24-Oct-07
Monosodium urate (crystals)
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SYNONYMS MSU (crystals)
Uric Acid (crystals)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Inflammasome
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ALX-400-047-M002   2 mg 90.00 USD Add To Cart
Product Specification
FORMULA: C5H4N4O3 . Na
MW: 168.1 . 23.0
CAS NUMBER: 1198-77-2
RTECS: YU7062000
CONCENTRATION: 5mg/ml after reconstitution.
PURITY DETAIL: Not determined. Degradation of the crystals with uricase (which breaks purines down into a very soluble product called allantoin) inhibits its activity.
RECONSTITUTION: Reconstitute with PBS.
ACTIVITY: Stimulates the caspase-1 activating NALP3 inflammasome, resulting in the production of active IL-1β and IL-18.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Vortex before use.

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Product Description
Specially crystallized and tested on biological activity.
Product Specific Literature References
Gout-associated uric acid crystals activate the NALP3 inflammasome: F. Martinon, et al.; Nature 440, 237 (2006) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Activation of THP-1 cells by uric acid:

1. THP-1 cells are cultured in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum
    (FCS), 50μM β-mercaptoethanol, 2mM L-glutamine and penicillin/streptomycin. THP-1 cells are grown
    in suspension to a density of 1.5x106 cells/ml in tissue culture flasks.
2. Treat THP-1 cells for 3 hours with 0.5µM PMA (Prod. No. ALX-445-004) the day before stimulation.
3. Wash cells with PBS and plate them in a 12-well plate at a density of 6x105 cells/well in RPMI medium
    (this increases the phagocytic properties of the cells and induces a constitutive production of proIL-1β).
4. The next day, wash cells with PBS and add 500µl Optimem (Invitrogen).
5. Stimulate the cells for 6 hours by addition of 50-200µg/ml of the suspension of uric acid (vortex the
    suspension just before use).
6. As a positive control, 5mM of ATP or 50µg/ml of LPS from E. coli, Serotype R515 (Re) (TLRgrade™)
    (liquid) (Prod. No. ALX-581-007) are incubated for 6 hours.

Detection of the mature IL-1β in the supernatant of uric acid-activated THP-1 cells:

1. Collect the supernatant of each well (500µl). Add 500µl methanol and 125µl chloroform.
2. Vortex and centrifuge for 5 min. at 13’000 rpm.
3. Remove the upper phase.
4. Add 500µl methanol and centrifuge for 5 min. at 13’000 rpm.
5. Remove the supernatant, dry the pellet for 5 min. at 50°C.
6. Add 60µl of 1x loading buffer and DTT (final concentration of 0.1M).
7. Mix and boil before loading on a 15% SDS-gel and transfer onto nitrocellulose membrane. The mature
    IL-1β is detected using the cleaved IL-1β (Asp116) antibody (Cell Signaling).

--> Note: Detection of IL-1β in the supernatant of uric acid-activated THP-1 cells can also be performed by ELISA.
--> Note: Sonication of MSU improves its biological activity in murine cells.
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ALX-385-016 Revised 02-Jun-08
Morin
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SYNONYMS 2’,3,4’,5,7-Pentahydroxyflavone
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Flavonols
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ALX-385-016-G001   1 g 16.00 USD Add To Cart
Product Specification
FORMULA: C15H10O7
MW: 302.2
CAS NUMBER: 480-16-0
MERCK INDEX: 14: 6269
RTECS: LK8749000
PURITY: ≥85%
APPEARANCE: White to brown powder.
SOLUBILITY: Soluble in methanol or 100% ethanol; slightly soluble in ether or acetic acid.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HAZARD: IRRITANT.

Product Description
Antioxidant flavonoid. Shows anti-proliferative and antitumor properties. Induces apoptosis. Induces cell cycle arrest at the G2/M phase. Anti-inflammatory compound. Suppresses NF-κB activation. Induces lipid peroxidation and DNA strand breaks.
Product Specific Literature References
Structure-antioxidant activity relationships of flavonoids and phenolic acids: C.A. Rice-Evans, et al.; Free Radical Biol. & Med. 20, 933 (1996), (Review) Abstract
Lipid peroxidation and DNA damage induced by morin and naringenin in isolated rat liver nuclei: S. C. Sahu & G. C. Gray; Food Chem. Toxicol. 35, 443 (1997) Abstract
Morin inhibits the growth of human leukemia HL-60 cells via cell cycle arrest and induction of apoptosis through mitochondria dependent pathway: H.M. Kuo, et al.; Anticancer Res. 27, 395 (2007) Abstract
Morin (3,5,7,2’,4’-Pentahydroxyflavone) abolishes nuclear factor-kappaB activation induced by various carcinogens and inflammatory stimuli, leading to suppression of nuclear factor-kappaB-regulated gene expression and up-regulation of apoptosi: S.K. Manna, et al.; Clin. Cancer Res. 13, 2290 (2007) Abstract
Further Categories Containing This Product:
NF-kB Pathway Inhibitors • Natural Products - Anti-inflammatory Agents • Antitumor Agents (Anti-proliferative) • Natural Products - NF-kB Pathway Inhibitors • Natural Products - Antitumor Reagents • Natural Products - Apoptosis Inducers & Inhibitors • Natural Products - Antioxidants • Cell Cycle Blockers & Inhibitors / Related Products • Natural Products for Cell Cycle Research • Lipid Peroxidation
 
 
ALX-385-012 Revised 19-Nov-07
Myricetin
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