ALEXIS Biochemicals: Polyclonal Antibody to Shc

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ALX-804-358

Revised 18-Jul-08

Monoclonal Antibody to Shc (phosphorylated) (pSer36) (6E10)
SYNONYMS	anti-Shc Transforming Protein 1 (phosphorylated) (pSer³⁶) MAb (6E10) anti-SH2 Domain Protein C1 (phosphorylated) (pSer³⁶) MAb (6E10) anti-Shc1 (phosphorylated) (pSer³⁶) MAb (6E10)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	SH2 & SH3 Domains

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-358-C100		100 µg	453.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Dog
CLONE:	6E10
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min, RT).
IMMUNOGEN:	Synthetic peptide corresponding to aa 33-39 (E³³LPpSPSA³⁹) of shc phosphorylated at Ser³⁶.
SPECIFICITY:	Recognizes human, mouse and dog shc phosphorylated at Ser³⁶. Does not cross-react with non-phosphorylated shc.
APPLICATION:	Western Blot (1µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride. Immunoprecipitation of shc prior to detection with MAb to Shc (phosphorylated) (pSer36) (6E10) is recommended.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL INCLUDED:	yes

Further Categories Containing This Product:

Phospho-specific Antibodies • Shc & Other Ras Adaptors / Related Products • Monoclonal Antibodies

ALX-804-357

Revised 30-May-08

Monoclonal Antibody to Shc (phosphorylated) (pTyr239/240) (1E3)
SYNONYMS	anti-Shc Transforming Protein 1 (phosphorylated) (pTyr^239/240) MAb (1E3) anti-SH2 Domain Protein C1 (phosphorylated) (pTyr^239/240) MAb (1E3) anti-Shc1 (phosphorylated) (pTyr^239/240) MAb (1E3)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	SH2 & SH3 Domains

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-357-C100		100 µg	453.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Dog
CLONE:	1E3
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:	Synthetic peptide corresponding to aa 236-243 (D²³⁶HQpYpYNDF²⁴³) of shc phosphorylated at Tyr²³⁹ and Tyr²⁴⁰.
SPECIFICITY:	Recognizes human, mouse and dog p46shc, p52shc and p66shc when phosphorylated at Tyr^239/240. Does not cross-react with non-phosphorylated shc.
APPLICATION:	Immunohistochemistry Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL:	Included (Prod. No. ALX-840-025).

Further Categories Containing This Product:

Phospho-specific Antibodies • Shc & Other Ras Adaptors / Related Products • Monoclonal Antibodies

ALX-210-535

Revised 31-Oct-06

Polyclonal Antibody to Shc
SYNONYMS	anti-Shc Transforming Protein 1 PAb anti-SH2 Domain Protein C1 PAb anti-Shc1 PAb
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	SH2 & SH3 Domains

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-535-R100		100 µl	220.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	From rabbit.
PURITY:	Liquid. Neat serum containing 0.05% sodium azide.
IMMUNOGEN:	Recombinant human Shc portion (aa 1180-1620).
SPECIFICITY:	Recognizes human, mouse and hamster p46shc, p52shc and p66shc isoforms of phosphorylated and unphosphorylated Shc.
APPLICATION:	ELISA Immunoprecipitation Western Blot
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

There are 3 alternatively spliced isoforms of the Shc adapter pro-tein: p46SHC, p52SHC and p66SHC, that link activated growth factor receptors to signalling pathway. Isoform p46SHC and p52SHC, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS com-plex and are implicated in mitogenic signalling. They function as initiators of the Ras signalling cascade in various non-neuronal systems. The isoform p66SHC does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. It acts as a downstream target of the tumor suppressor p53 and is indispen-sable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis.

Further Categories Containing This Product:

Phospho-specific Antibodies • Shc & Other Ras Adaptors / Related Products • Polyclonal Antibodies

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