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cAMP Pathways
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ALX-270-039 Revised 15-Nov-06
A-3 . hydrochloride
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SYNONYMS N-(2-Aminoethyl)-5-chloronaphthalene-1-sulfonamide . HCl
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKA Inhibitors
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ALX-270-039-M010   10 mg 65.00 USD Add To Cart
ALX-270-039-M050   50 mg 260.00 USD Add To Cart
Product Specification
FORMULA: C12H13ClN2O2S . HCl
MW: 284.8 . 36.5
PURITY: ≥98%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO or 100% ethanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Inhibitor of cAMP- and cGMP-dependent protein kinase (PKA and PKG), protein kinase C (PKC), casein kinase I and II, and myosin light chain kinase.
Product Specific Literature References
Naphthalenesulfonamides as calmodulin antagonists and protein kinase inhibitors: M. Inagaki, et al.; Mol. Pharmacol. 29, 577 (1986) Abstract
Further Categories Containing This Product:
Myosin Light Chain Kinase Inhibitors • PKC Inhibitors • Casein Kinase Inhibitors • PKG Inhibitors
 
 
ALX-480-011 Revised 17-Jan-05
Adenosine 3',5'-cyclic Monophosphate . sodium salt
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SYNONYMS Cyclic AMP . Na
cAMP . Na
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY cAMP Derivatives
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ALX-480-011-G001   1 g 195.00 USD Add To Cart
ALX-480-011-M100   100 mg 38.00 USD Add To Cart
ALX-480-011-M250   250 mg 70.00 USD Add To Cart
Product Specification
FORMULA: C10H11N5O6P . Na
MW: 328.2 . 23.0
CAS NUMBER: 37839-81-9
MERCK INDEX: 14: 2708
PURITY: ≥98%
APPEARANCE: White solid.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Key regulator of many cellular reactions. Naturally occurring activator of cAMP-dependent protein kinase (PKA).
Product Specific Literature References
TGF-beta 1 and cyclic AMP promote apoptosis in resting human B lymphocytes: J. Lomo, et al.; J. Immunol. 154, 1634 (1995) Abstract
Further Categories Containing This Product:
PKA Activators
 
 
ALX-480-085 Revised 05-Sep-07
Adenosine-3’,5’-cyclic Monophosphothioate, Rp-Isomer . sodium salt
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SYNONYMS Rp-cAMPS . Na
cAMPS . Na, Rp-Isomer
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY cAMP Derivatives
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ALX-480-085-M001   1 mg 120.00 USD Add To Cart
ALX-480-085-M005   5 mg 490.00 USD Add To Cart
Product Specification
FORMULA: C10H11N5NaO5PS
MW: 367.3
CAS NUMBER: 73208-40-9
PURITY: ≥99% (low adenosine, cAMP and Sp-cAMPS content)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in water or aqueous buffers.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
For application and literature references see the triethylammonium salt (Prod. No. ALX-480-012).
Further Categories Containing This Product:
PKA Inhibitors
 
 
ALX-480-012 Revised 27-Apr-06
Adenosine 3',5'-cyclic Monophosphothioate, Rp-Isomer . triethylammonium salt
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SYNONYMS Rp-cAMPS . TEA
cAMPS . TEA, Rp-Isomer
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY cAMP Derivatives
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ALX-480-012-M001   1 mg 110.00 USD Add To Cart
ALX-480-012-M005   5 mg 460.00 USD Add To Cart
Product Specification
FORMULA: C10H12N5O5PS . C6H16N
MW: 345.5 . 101.0
CAS NUMBER: 151837-09-1
PURITY: ≥99%
APPEARANCE: White lyophilized solid.
SOLUBILITY: Soluble in water
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Competitive inhibitor of protein cAMP-dependent protein kinase type I and II (cAMP antagonist). Resistant against mammalian cyclic nucleotide-dependent phosphodiesterases. Significantly more lipophilic as compared to cAMP and thus membrane permeable in many biological systems.
Product Specific Literature References
A mechanistic and kinetic analysis of the interactions of the diastereoisomers of adenosine 3',5'-(cyclic)phosphorothioate with purified cyclic AMP-dependent protein kinase: J.D. Rothermel; Biochem. J. 251, 757 (1988) Abstract
Probing the cyclic nucleotide binding sites of cAMP-dependent protein kinases I and II with analogs of adenosine 3',5'-cyclic phosphorothioates: W.R.G. Dostmann, et al.; J. Biol. Chem. 265, 10484 (1990) Abstract; Full Text
Inhibition of cGMP-dependent protein kinase by (Rp)-guanosine 3',5'- monophosphorothioates: E. Butt, et al.; FEBS Lett. 263, 47 (1990) Abstract
Regulation of kainate receptors by cAMP-dependent protein kinase and phosphatases: L.Y. Wang, et al.; Science 253, 1132 (1991) Abstract
Stimulation of the ERK pathway by GTP-loaded Rap1 requires the concomitant activation of Ras, protein kinase C, and protein kinase A in neuronal cells: T. Bouschet, et al.; J. Biol. Chem. 278, 4778 (2003) Abstract; Full Text
Further Categories Containing This Product:
PKA Inhibitors
 
 
ALX-480-086 Revised 30-Aug-06
Adenosine 3',5'-cyclic Monophosphothioate, Sp-Isomer . sodium salt
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SYNONYMS Sp-cAMPS . Na
cAMPS . Na, Sp-Isomer
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY cAMP Derivatives
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ALX-480-086-M001   1 mg 75.00 USD Add To Cart
ALX-480-086-M005   5 mg 300.00 USD Add To Cart
Product Specification
FORMULA: C10H11N5NaO5PS
MW: 367.3
CAS NUMBER: 717794-13-5
PURITY: ≥99% (low adenosine, cAMP and Rp-cAMPS content)
APPEARANCE: White to off-white lyophilized powder.
SOLUBILITY: Soluble in water or aqueous buffers.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
For application and literature references see the triethylammonium salt (Prod. No. ALX-480-013).
Further Categories Containing This Product:
PKA Activators
 
 
ALX-480-013 Revised 10-Feb-05
Adenosine 3',5'-cyclic Monophosphothioate, Sp-Isomer . triethylammonium salt
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SYNONYMS Sp-cAMPS . TEA
cAMPS . TEA, Sp-Isomer
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY cAMP Derivatives
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ALX-480-013-M001   1 mg 75.00 USD Add To Cart
ALX-480-013-M005   5 mg 298.00 USD Add To Cart
ALX-480-013-M010   10 mg 580.00 USD Add To Cart
Product Specification
FORMULA: C16H27N6O5PS
MW: 446.5
CAS NUMBER: 93602-66-5
PURITY: ≥99%
APPEARANCE: White lyophilized solid.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Activator of cAMP-dependent protein kinase (PKA) (cAMP agonist). Considerably higher resistance against cyclic nucleotide-dependent phosphodiesterases as compared to N6,O2'-dibutyryladenosine 3',5'-cyclic monophosphate (dibutyryl-cAMP) (Prod. No. ALX-480-045) or 8-bromoadenosine-3',5'-cyclic monophosphate (8-Br-cAMP) (Prod. No. ALX-480-007). No metabolic side effects. Membrane permeable in many biological systems.
Product Specific Literature References
Probing the cyclic nucleotide binding sites of cAMP-dependent protein kinases I and II with analogs of adenosine 3',5'-cyclic phosphorothioates: W.R.G. Dostmann, et al.; J. Biol. Chem. 265, 10484 (1990) Abstract; Full Text
Cyclic AMP antagonist Rp-cAMPS inhibits amylase exocytosis from saponin- permeabilized parotid acini: T. Takuma & T. Ichida; J. Biochem. 110, 292 (1991) Abstract
Second messenger signaling in the regulation of cytosolic pH and DNA synthesis by parathyroid hormone (PTH) and PTH-related peptide in osteoblastic osteosarcoma cells: role of Na+/H+ exchange: T. Sugimoto, et al.; J. Cell Physiol. 152, 28 (1992) Abstract
The activation of cAMP-dependent protein kinase is directly linked to the stimulation of bone resorption by parathyroid hormone: H. Kaji, et al.; BBRC 182, 1356 (1992) Abstract
Intracellular cyclic AMP not calcium, determines the direction of vesicle movement in melanophores: direct measurement by fluorescence ratio imaging: P.J. Sammak, et al.; J. Cell. Biol. 117, 57 (1992) Abstract
Effects of activators and inhibitors of protein kinase A on increases in quantal size at the frog neuromuscular junction: W. Van Der Kloot & D.D. Branisteanu; Pfluegers Arch. 420, 336 (1992) Abstract
Further Categories Containing This Product:
PKA Activators
 
 
ALX-630-088 Revised 03-Apr-08
Adenylate Cyclase Toxin
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SYNONYMS AC Toxin
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Other Toxins
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ALX-630-088-C050   50 µg 290.00 USD Add To Cart
Product Specification
MW: ~177kDa.
SOURCE/HOST: Recombinant Bordetella pertussis adenylate cyclase toxin produced in E. coli.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥75% (SDS-PAGE)
APPEARANCE: Lyophilized.
SOLUBILITY: Soluble in water.
RECONSTITUTION: Reconstitute with 50µl sterile distilled water to get a solution in 20mM HEPES, pH 7.5, containing 8M urea. The resulting suspension should be made uniform by gentle mixing prior to use.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: After reconstitution, store at concentrations higher than 100µg/ml to reduce adherence to the container surface.
HANDLING: After reconstitution, prepare aliquots and store at -20°C.
HAZARD: TOXIC.
Product Description
Important virulence factor of Bordetella pertussis and a research tool for manipulation of cAMP levels in mammalian cells. Has the ability to interact with target cells, insert into the cytoplasmic membrane and deliver its adenylate cyclase enzymatic domain to the cell interior.
Product Specific Literature References
[1] Calmodulin activates prokaryotic adenylate cyclase: J. Wolff, et al.; PNAS 77, 3841 (1980) Abstract
[2] Phagocyte impotence caused by an invasive bacterial adenylate cyclase: D.L. Confer & J.W. Eaton; Science 217, 948 (1982) Abstract
[3] Virulence factors of Bordetella pertussis: A. Weiss & E.L. Hewlett; Ann. Rev. Microbiol. 40, 661 (1986) Abstract
[4] The calmodulin-sensitive adenylate cyclase of Bordetella pertussis: cloning and expression in Escherichia coli: P. Glaser, et al.; Mol. Microbiol. 2, 19 (1988) Abstract
[5] Adenylate cyclase toxin from Bordetella pertussis. Identification and purification of the holotoxin molecule: E.L. Hewlett, et al.; J. Biol. Chem. 264, 19379 (1989) Abstract
[6] Bordetella pertussis adenylate cyclase toxin. Structural and functional independence of the catalytic and hemolytic activities: H. Sakamoto, et al.; J. Biol. Chem. 267, 13598 (1992) Abstract; Full Text
[7] Characterization of adenylate cyclase toxin from a mutant of Bordetella pertussis defective in the activator gene, cyaC: E.L. Hewlett, et al.; J. Biol. Chem. 268, 7842 (1993) Abstract; Full Text
[8] Calmodulin-activated bacterial adenylate cyclases as virulence factors: M. Mock & A. Ullmann; Trends in Microbiol. 1, 187 (1993) Abstract
[9] Adenylate cyclase toxin (CyaA) of Bordetella pertussis. Evidence for the formation of small ion-permeable channels and comparison with HlyA of Escherichia coli: R. Benz, et al.; J. Biol. Chem. 269, 27231 (1994) Abstract; Full Text
[10] Internal lysine palmitoylation in adenylate cyclase toxin from Bordetella pertussis: M. Hackett, et al.; Science 266, 433 (1994) Abstract
[11] Adenylate cyclase toxin from Bordetella pertussis produces ion conductance across artificial lipid bilayers in a calcium- and polarity- dependent manner: G. Szabo, et al.; J. Biol. Chem. 269, 22496 (1994) Abstract; Full Text
[12] Hemolytic, but not cell-invasive activity, of adenylate cyclase toxin is selectively affected by differential fatty-acylation in escherichia coli: M. Hackett, et al.; J. Biol. Chem. 270, 20250 (1995) Abstract; Full Text
[13] Bordetella pertussis adenylate cyclase toxin: a versatile screening tool: N. Dautin, et al.; Toxicon 40, 1383 (2002), (Review) Abstract
[14] The adenylate cyclase toxin from Bordetella pertussis--a novel promising vehicle for antigen delivery to dendritic cells: M. Simsova, et al.; Int. J. Med. Microbiol. 239, 571 (2004), (Review) Abstract
[15] Bordetella adenylate cyclase toxin: a swift saboteur of host defense: J. Vojtova, et al.; Curr. Opin. Microbiol. 9, 69 (2006) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Enzymatic activity: The enzymatic activity of this preparation is >238µmol cAMP/min/mg of protein as determined by a modification of the method of Hewlett et al. [5].
Toxicity: The toxicity of this preparation is >63µmol cAMP/mg of J774A.1 cell protein as determined by a modification of the method of Hewlett et al. [7].
Further Categories Containing This Product:
Enzymes • Adenylyl Cyclase Activators
 
 
ALX-850-013 Revised 24-Feb-05
cAMP EIA Kit
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY cAMP Derivatives
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ALX-850-013-KI01   1 Kit 412.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the measurement of cAMP.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
ELISA & EIA Kits
 
 
ALX-202-027 Revised 09-Aug-06
cAMP-dependent Protein Kinase (bovine heart) (catalytic subunit)
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SYNONYMS Protein Kinase A (bovine heart) (catalytic subunit)
PKA (bovine heart) (catalytic subunit)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKA
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ALX-202-027-U500   500 U 250.00 USD Add To Cart
Product Specification
MW: ~40kDa.
EC: 2.7.1.37
SOURCE/HOST: Isolated from bovine heart.
CONCENTRATION: 20µg/ml.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 20mM MES, pH 6.5, containing 0.1mM EDTA, 30mM β-mercaptoethanol, 100mM sodium chloride and 50% ethylene glycol.
SPECIFIC ACTIVITY: ~1000 Units/µg using histone as the substrate. One unit is defined as the amount of enzyme that transfers 1pmol phosphate to histone per min. at 30°C.
APPLICATION: For studying PKA phosphorylation in vitro.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Do not freeze/thaw.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link: cAMP-dependent Protein Kinase (bovine) α catalytic subunit (P00517), β-1 catalytic subunit (P05131), β-2 catalytic subunit (P24256)
AfCS Signalling Gateway link: cAMP-dependent Protein Kinase (mouse) α catalytic subunit (A001914), β catalytic subunit (A001815), γ catalytic subunit (A001916)
Further Categories Containing This Product:
Enzymes • Natural Proteins
 
 
ALX-151-016 Revised 17-Jan-05
cAMP-dependent Protein Kinase Inhibitor (5-22) amide
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SYNONYMS PKA Inhibitor (5-22) amide
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKA Inhibitors
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ALX-151-016-MC05   0.5 mg 110.00 USD Add To Cart
ALX-151-016-M001   1 mg 180.00 USD Add To Cart
Product Specification
SEQUENCE: H-Thr-Thr-Tyr-Ala-Asp-Phe-Ile-Ala-Ser-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile-NH2
FORMULA: C84H137N29O26
MW: 1969.2
PURITY: ≥97%
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Potent inhibitor of cAMP-dependent protein kinase (PKA).
Product Specific Literature References
A potent synthetic peptide inhibitor of the cAMP-dependent protein kinase: H.-C. Cheng, et al.; J. Biol. Chem. 261, 989 (1986) Abstract; Full Text
Primary structural determinants essential for potent inhibition of cAMP- dependent protein kinase by inhibitory peptides corresponding to the active portion of the heat-stable inhibitor protein: D.B. Glass, et al.; J. Biol. Chem. 264, 8802 (1989) Abstract; Full Text
Further Categories Containing This Product:
Peptides