ALEXIS Biochemicals: L-655,238

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Annexins / Related Products

Ceramides / Cerebrosides / Sphingolipids / Related Products

Diacylglycerol Kinases / Related Products

Diacylglycerol Lipase [DAGL] & Monoacylglycerol Lipase [MGL] / Related Products

Eicosanoids / Related Products

Mono- & Diacylglycerols

Phospholipase A2 [PLA2] / Related Products

Phospholipase C / Related Products

Phospholipase D / Related Products

Phospholipids / Related Products

Platelet Activating Factors [PAF] / Related Products

Lipid Signalling Pathways Other Products

ALX-162-006

Revised 07-Apr-08

Melittin (natural)
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products - Protein Kinase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-162-006-M001		1 mg	40.00 USD
ALX-162-006-M005		5 mg	160.00 USD

Product Specification

SEQUENCE:	H-Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH₂
FORMULA:	C₁₃₁H₂₂₉N₃₉O₃₁
MW:	2846.5
CAS NUMBER:	37231-28-0
MERCK INDEX:	14: 5824
RTECS:	OS3960000
SOURCE/HOST:	Isolated from bee venom.
PURITY:	≥85% (HPLC)
APPEARANCE:	Off-white to faint tan powder.
PURITY DETAIL:	Phospholipase A₂impurity: <5 units per mg solid.
SOLUBILITY:	Soluble in PBS (5mg/ml) or water (5mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Very hygroscopic. Protect from light.
HAZARD:	HARMFUL.

Product Description

Causes disruption of normal cellular activity and cell lysis. Binds calmodulin in a calcium-dependent manner. Activates phospholipase A₂ (PLA₂) and inhibits protein kinase C (PKC) by binding to the catalytic domain in a Mg-ATP sensitive manner. Antirheumatic.

Product Specific Literature References

Rapid purification of calmodulin and S-100 protein by affinity chromatography with melittin immobilized to sepharose: R.L. Kincaid & C.C. Coulson; BBRC 133, 256 (1985) Abstract
Comparison of S100b protein with calmodulin: interactions with melittin and microtubule-associated tau proteins and inhibition of phosphorylation of tau proteins by protein kinase C: J. Baudier, et al.; Biochemistry 26, 2886 (1987) Abstract
Possible mechanisms of action of cobra snake venom cardiotoxins and bee venom melittin: J.E. Fletcher & M.S. Jiang; Toxicon. 31, 669 (1993) Abstract
Melittin and phospholipase A2 from bee (Apis mellifera) venom cause necrosis of murine skeletal muscle in vivo: C.L. Ownby, et al.; Toxicon. 35, 67 (1997) Abstract
Melittin binds to secretory phospholipase A2 and inhibits its enzymatic activity: S.S. Saini, et al.; BBRC 238, 436 (1997) Abstract
Phospholipase A2-independent Ca2+ entry and subsequent apoptosis induced by melittin in human MG63 osteosarcoma cells: S.T. Chu, et al.; Life Sci. 80, 364 (2007) Abstract

Further Categories Containing This Product:

Calmodulin Other Products • PKC Inhibitors • Peptides • PLA2 Activators

ALX-162-007

Revised 12-May-05

Melittin (synthetic)
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products - Protein Kinase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-162-007-MC05		0.5 mg	70.00 USD
ALX-162-007-M001		1 mg	120.00 USD

Product Specification

SEQUENCE:	H-Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH₂
FORMULA:	C₁₃₁H₂₂₉N₃₉O₃₁
MW:	2846.5
CAS NUMBER:	37231-28-0
MERCK INDEX:	14: 5824
SOURCE/HOST:	Synthetic.
PURITY:	≥97%
APPEARANCE:	White lyophilized powder.
SOLUBILITY:	Soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.
HAZARD:	TOXIC.

Product Description

For technical information see melittin (natural) (Prod. No. ALX-162-006).

Further Categories Containing This Product:

PLA2 Activators • Calmodulin Other Products • PKC Inhibitors • Peptides

ALX-165-007

Revised 05-Dec-04

Phospholipase A2 Activating Peptide
SYNONYMS	PLAP
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	PLA2 Activators

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-165-007-MC01		0.1 mg	40.00 USD
ALX-165-007-M001		1 mg	140.00 USD

Product Specification

SEQUENCE:	H-Glu-Ser-Pro-Leu-Ile-Ala-Lys-Val-Leu-Thr-Thr-Glu-Pro-Pro-Ile-Ile-Thr-Pro-Val-Arg-Arg-OH
FORMULA:	C₁₀₆H₁₈₄N₂₈O₃₀
MW:	2330.8
PURITY:	≥97%
APPEARANCE:	White lyophilized powder.
SOLUBILITY:	Soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Peptide fragment from phospholipase A₂(PLA₂)activating protein (PLAP) that activates phospholipase A₂in a dose-dependent manner. The amino acid sequence bears considerable homology to melittin.

Product Specific Literature References

Cloning of a phospholipase A2-activating protein: M.A. Clark, et al.; PNAS 88, 5418 (1991) Abstract

Further Categories Containing This Product:

Peptides

ALX-201-116

Revised 21-Jan-08

Annexin V (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Annexin V / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-116-C100		100 µg	194.00 USD

Product Specification

MW:	~52kDa (SDS-PAGE).
SOURCE/HOST:	Produced in E. coli. Recombinant human annexin V fused to GST.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Affinity purified.
FORMULATION:	Lyophilized. Contains PBS, pH 7.4, and 0.02% sodium azide.
RECONSTITUTION:	Reconstitute with 100µl sterile water.
SPECIFICITY:	Binds to phosphatidylserine (PS).
BIOLOGICAL ACTIVITY:	Exhibits anti-phospholipase activity.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Reconstituted solutions can be kept at 4°C under sterile conditions for one week or should be frozen in aliquots at -20°C. Repeated freeze/thaw cycles (>3) may result in an adverse effect on the activity of annexin V.
HANDLING:	Avoid freeze/thaw cycles.

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P08758: Annexin V (human) AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-209-002

Revised 02-Aug-06

Annexin V (human) (recombinant) (Biotin)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Annexin V / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-209-002-T020		20 tests	106.00 USD
ALX-209-002-T100		100 tests	352.00 USD
ALX-209-002-T300		300 tests	531.00 USD

Product Specification

MW:	~35.8kDa.
QUANTITY:	20 tests/100µl; 100 tests/500µl; 300 tests/1'500µl.
PURITY:	≥98% (SDS-PAGE, HPLC)
FORMULATION:	Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY:	Binds to phosphatidylserine (PS).
APPLICATION:	Detection of apoptotic cells by flow cytometry.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C

Product Specific Literature References

[1] Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers: H.A. Andree, et al.; J. Biol. Chem. 265, 4923 (1990) Abstract; Full Text
[2] Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages: V.A. Fadok, et al.; J. Immunol. 148, 2207 (1992) Abstract
[3] Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis: G. Koopman, et al.; Blood 84, 1415 (1994) Abstract
[4] Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro: C.H. Homburg, et al.; Blood 85, 532 (1995) Abstract
[5] A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth. 184, 39 (1995) Abstract
[6] Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl: S. Martin, et al.; J. Exp. Med. 182, 1545 (1995) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[8] CD95 engagement releases calcium from intracellular stores of long term activated, apoptosis-prone gammadelta T cells: P. Rovere, et al.; J. Immunol. 156, 4631 (1996) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Flow Cytometry Protocol:
- Brief centrifugation before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down
- Resuspend cells in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered
through 0.2µm pore filter); adjust cell density to 2-5x10⁵/ml
- Take 195µl cell suspension and add 5µl Annexin V-Biotin
- Mix and incubate 15 minutes at room temperature
- Wash cells 2x in binding buffer
- Resuspend in binding buffer
- Add Streptavidin-FITC
- Mix and incubate for 30 minutes in the dark at room temperature
- Wash cells 1x in binding buffer and resuspend in 190µl binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1mg/ml)
- FACS analysis

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281 : Annexin V (mouse)

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins • Biotinylated Reagents Other Products

ALX-209-250

Revised 08-Aug-08

Annexin V (human) (recombinant) (FITC)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Annexin V / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-209-250-T020		20 tests	106.00 USD
ALX-209-250-T100		100 tests	352.00 USD
ALX-209-250-T300		300 tests	531.00 USD

Product Specification

MW:	~35.8kDa.
SOURCE/HOST:	Produced in E. coli.
QUANTITY:	20 tests/100µl; 100 tests/500µl; 300 tests/1'500µl.
PURITY:	≥98% (SDS-PAGE, HPLC)
FORMULATION:	Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY:	Binds to phosphatidylserine (PS).
APPLICATION:	Detection of apoptotic cells by flow cytometry.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	After opening, prepare aliquots and store at -20°C.

Product Description

FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm).

Product Specific Literature References

[1] Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers: H.A. Andree, et al.; J. Biol. Chem. 265, 4923 (1990) Abstract; Full Text
[2] Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages: V.A. Fadok, et al.; J. Immunol. 148, 2207 (1992) Abstract
[3] Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis: G. Koopman, et al.; Blood 84, 1415 (1994) Abstract
[4] Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro: C.H. Homburg, et al.; Blood 85, 532 (1995) Abstract
[5] A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth. 184, 39 (1995) Abstract
[6] Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl: S. Martin, et al.; J. Exp. Med. 182, 1545 (1995) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[8] CD95 engagement releases calcium from intracellular stores of long term activated, apoptosis-prone gammadelta T cells: P. Rovere, et al.; J. Immunol. 156, 4631 (1996) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Flow Cytometry Protocol:
- Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl₂; filtered through
0.2µm pore filter).
- Brief centrifugation of annexin V-FITC before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down.
- Resuspend cells in binding buffer; adjust cell density to 2-5x10⁵/ml
- Take 195µl cell suspension and add 5µl annexin V-FITC- mix and incubate 10 min. in the dark at room temperature
- Wash cells 1x with PBS and resuspend in 190µl binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)
- FACS analysis

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281 : Annexin V (mouse)

ALX-209-251	Annexin V (human) (recombinant) (R-PE)
ALX-209-252	Annexin V (human) (recombinant) (APC)
ALX-209-256	Annexin V (human) (recombinant) (FITC)
ALX-209-258	Annexin V (human) (recombinant) (ATTO 488)

ALX-209-256	Annexin V (human) (recombinant) (FITC)
ALX-209-252	Annexin V (human) (recombinant) (APC)
ALX-209-250	Annexin V (human) (recombinant) (FITC)
ALX-209-258	Annexin V (human) (recombinant) (ATTO 488)

ALX-209-250	Annexin V (human) (recombinant) (FITC)
ALX-209-251	Annexin V (human) (recombinant) (R-PE)
ALX-209-256	Annexin V (human) (recombinant) (FITC)
ALX-209-258	Annexin V (human) (recombinant) (ATTO 488)

MW:	~35.8kDa.
SOURCE/HOST:	Produced in E. coli.
QUANTITY:	20 tests/100µl; 100 tests/500µl.
PURITY:	≥98% (SDS-PAGE, HPLC)
FORMULATION:	Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY:	Binds to phosphatidylserine (PS).
APPLICATION:	Detection of apoptotic cells by flow cytometry.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Do not freeze. Protect from light.