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Signal Transduction
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ALX-522-016 Revised 28-Apr-06
CD40 (human):Fc (human) (recombinant)
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SYNONYMS TNFRSF 5 (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154]/Related Products
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ALX-522-016-C050   50 µg 355.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~54kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The cysteine-rich region (aa 21-193) of human CD40 is fused to the Fc portion of human IgG1.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds human and mouse CD40L (CD154).
APPLICATION: Can be used together with anti-human IgG1 PAb to detect membrane-bound human or mouse CD40L. CD40:Fc should be used at concentrations between 1 and 100µg/ml depending on the level of CD40L surface expression.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P25942: CD40 (human) (precursor)
AfCS Signalling Gateway link A000031: CD40 (mouse)
MANUFACTURER

Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins/Fusion Proteins
 
 
ALX-850-262 Revised 11-Apr-08
CD40, Soluble (human) ELISA Kit
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SYNONYMS TNFSF 5, Soluble (human) ELISA Kit
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154]/Related Products
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ALX-850-262-KI01   1 Kit 619.00 USD Add To Cart
Product Specification
SENSITIVITY: 6.92pg/ml (range 7.8 to 500 pg/ml)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative determination of human soluble CD40 in cell culture supernatants, serum and other body fluids.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
CD Kits & Sets • ELISA & EIA Kits
 
 
ALX-522-076 Revised 19-Apr-06
Fc (human):CD40L, Soluble (human) (recombinant)
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SYNONYMS Fc (human):CD154, Soluble (human) (recombinant)
Fc (human):TNFSF 5, Soluble (human) (recombinant)
Fc (human):gp39, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154]/Related Products
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ALX-522-076-C010   10 µg 400.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
MW: ~58kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa).
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; BioWhittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds to human CD40.
BIOLOGICAL ACTIVITY: Stimulates the proliferation of human dendritic cells. Note: Does not require an enhancer.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins • CD Proteins
 
 
ALX-522-072 Revised 19-Apr-06
Fc (human):CD40L, Soluble (mouse) (recombinant)
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SYNONYMS Fc (human):CD154, Soluble (mouse) (recombinant)
Fc (human):TNFSF 5, Soluble (mouse) (recombinant)
Fc (human):gp39, Soluble (mouse) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154]/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-072-C010   10 µg 400.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~58kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa).
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds to human and mouse CD40.
BIOLOGICAL ACTIVITY: Stimulates the proliferation of mouse B cells and dendritic cells. Note: Does not require an enhancer.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins/Fusion Proteins
 
 
ALX-522-015 Revised 28-Apr-06
CD40L, Soluble (human) (recombinant)
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SYNONYMS CD154, Soluble (human) (recombinant)
TNFSF 5, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154]/Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-015-2010   2x10 µg 200.00 USD Add To Cart
ALX-522-015-6010 SuperPack 6x10 µg 410.00 USD Add To Cart
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Product Specification
SPECIES CROSSREACTIVITY:
Human
MW: ~18kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute each tube with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds to human CD40.
BIOLOGICAL ACTIVITY: Stimulates growth of B cells. The activity of rhsCD40L increases 1’000-fold (stimulation in the ng/ml range) in the presence of cross-linking enhancer (see Set Prod. No. ALX-850-064). For stimulation of mouse cells via CD40 use CD40L, Soluble (mouse) (recombinant) (Prod. No. ALX-522-070 or ALX-850-075).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Images
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Product Specific Literature References
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 12051 (1998) Abstract; Full Text
CD45 inhibits CD40L-induced microglial activation via negative regulation of the Src/p44/42 MAPK pathway: J. Tan, et al.; J. Biol. Chem. 275, 37224 (2000) Abstract; Full Text
Histamine polarizes human dendritic cells into Th2 cell-promoting effector dendritic cells: G. Caron, et al.; J. Immunol. 167, 3682 (2001) Abstract
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001) Abstract; Full Text
Synergy between CD40 ligation and IL-4 on fibroblast proliferation involves IL-4 receptor signaling: S.P. Atamas, et al.; J. Immunol. 168, 1139 (2002) Abstract
Pro-inflammatory effect of TWEAK/Fn14 interaction on human umbilical vein endothelial cells: N. Harada, et al.; BBRC 299, 488 (2002) Abstract
Suppression of IL-12 Production by Soluble CD40 Ligand: Evidence for Involvement of the p44/42 Mitogen-Activated Protein Kinase Pathway: M. Wittmann, et al.; J. Immunol. 168, 3793 (2002) Abstract
HIV-1 Nef intersects the macrophage CD40L signalling pathway to promote resting-cell infection: S. Swingler, et al.; Nature 424, 213 (2003) Full Text
Platelet-Activating Factor Mediates CD40-Dependent Angiogenesis and Endothelial-Smooth Muscle Cell Interaction: S. Russo, et al.; J. Immunol. 171, 5489 (2003) Abstract
Induction of Cell Cycle Arrest and Apoptosis by the Proteasome Inhibitor PS-341 in Hodgkin Disease Cell Lines Is Independent of Inhibitor of Nuclear Factor-kappaB Mutations or Activation of the CD30, CD40, and RANK Receptors: B. Zheng, et al.; Clin. Cancer Res. 10, 3207 (2004) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P29965: CD40L (human)
AfCS Signalling Gateway link A000536: CD40L (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins/Fusion Proteins
 
 
ALX-850-064 Revised 23-Apr-08
CD40L, Soluble (human) (recombinant) Set
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SYNONYMS TNFSF 5, Soluble (human) (recombinant) Set
CD154, Soluble (human) (recombinant) Set
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154]/Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-064-KI01   1 Set 440.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: Produced in E. coli. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG®-tag.
CONCENTRATION: CD40L: 0.1mg/ml after reconstitution.
Enhancer: 1mg/ml after reconstitution.
KIT/SET CONTAINS: 10µg of CD40L, Soluble (human) (recombinant) (Prod. No. ALX-522-015) and 2x50µg of Enhancer for Ligands (Prod. No. ALX-804-034), which increases the biological activity of rhsCD40L at least 1’000-fold.
PURITY: ≥95% (SDS-PAGE).
FORMULATION: Lyophilized. Contains PBS.
RECONSTITUTION: Reconstitute CD40L with 100µl and each vial of enhancer with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
SPECIFICITY: CD40L binds to human CD40.
APPLICATION: Can be used to activate human B cells and dendritic cells. Stimulates growth of human B cells. For stimulation of mouse cells via CD40 use MAb to CD40 (mouse) (FGK45) (Prod. No. ALX-805-046).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998) Abstract; Full Text
CD45 inhibits CD40L-induced microglial activation via negative regulation of the Src/p44/42 MAPK pathway: J. Tan, et al.; J. Biol. Chem. 275, 37224 (2000) Abstract; Full Text
CD40 induces apoptosis in carcinoma cells through activation of cytotoxic ligands of the tumor necrosis factor superfamily: A.G. Eliopoulos, et al.; Mol. Cell. Biol. 20, 5503 (2000) Abstract
Brain dendritic cells and macrophages/microglia in central nervous system inflammation: H.G. Fischer & G. Reichmann; J. Immunol. 166, 2717 (2001) Abstract; Full Text
Synergy between CD40 ligation and IL-4 on fibroblast proliferation involves IL-4 receptor signaling: S.P. Atamas, et al.; J. Immunol. 168, 1139 (2002) Abstract; Full Text
The novel IgD binding protein from Moraxella catarrhalis induces human B lymphocyte activation and Ig secretion in the presence of Th2 cytokines: A. Gjorloff Wingren, et al.; J. Immunol. 168, 5582 (2002) Abstract; Full Text
Platelet-Activating Factor Mediates CD40-Dependent Angiogenesis and Endothelial-Smooth Muscle Cell Interaction: S. Russo, et al.; J. Immunol. 171, 5489 (2003) Abstract
Induction of antigen-specific CTL by recombinant HIV trans-activating fusion protein-pulsed human monocyte-derived dendritic cells: Y. Tanaka, et al.; J. Immunol. 170, 1291 (2003) Abstract; Full Text
IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo: A. Soruri, et al.; J. Immunol. 170, 3306 (2003) Abstract; Full Text
CD40 induces human multiple myeloma cell migration via phosphatidylinositol 3-kinase/AKT/NF-kappa B signaling: Y.T. Tai, et al.; Blood 101, 2762 (2003) Abstract
TNF receptor-associated factor 6 is an essential mediator of CD40-activated proinflammatory pathways in monocytes and macrophages: L. Mukundan, et al.; J. Immunol. 174, 1018 (2005) Abstract; Full Text
Enhanced activation of human dendritic cells by inducible CD40 and Toll-like receptor-4 ligation: N. Lapteva, et al.; Cancer Res. 67, 10528 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION

CD40L (Prod. No. ALX-522-015) and Enhancer for Ligands (Prod. No. ALX-804-034) should be incubated together for 30 min. at room temperature prior to cell application. Effects are typically best seen with 0.5-10µg/ml of CD40L together with 1-2µg/ml of enhancer. This may vary by cell type and experimental conditions.

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
CD Kits & Sets • Receptor/Ligand + Enhancer Set
 
 
ALX-522-070 Revised 18-Apr-06
CD40L, Soluble (mouse) (recombinant)
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SYNONYMS CD154, Soluble (mouse) (recombinant)
TNFSF 5, Soluble (mouse) (recombinant)
gp39, Soluble (mouse) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154]/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-070-2010   2x10 µg 350.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~26kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute each tube with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds to human and mouse CD40.
BIOLOGICAL ACTIVITY: Stimulates the proliferation of mouse B cells and dendritic cells. The activity of rmsCD40L increases 1'000-fold (stimulation in the ng/ml range) in the presence of a cross-linking enhancer (see Set Prod. No. ALX-850-075).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins/Fusion Proteins
 
 
ALX-850-075 Revised 23-Apr-08
CD40L, Soluble (mouse) (recombinant) Set
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SYNONYMS CD154, Soluble (mouse) (recombinant) Set
TNFSF 5, Soluble (mouse) (recombinant) Set
gp39, Soluble (mouse) (recombinant) Set
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154]/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-075-KI01   1 Set 495.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
CONCENTRATION: CD40L: 0.1mg/ml after reconstitution.
Enhancer: 1mg/ml after reconstitution.
KIT/SET CONTAINS: 10µg of CD40L, Soluble (mouse) (recombinant) (Prod. No. ALX-522-070) and 2x50µg of Enhancer for Ligands (Prod. No. ALX-804-034) which increases the biological activity of CD40L at least 1’000-fold.
PURITY: ≥90% (SDS-PAGE).
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute CD40L with 100µl sterile water and each vial of enhancer with 50µl of sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
SPECIFICITY: CD40L binds to human and mouse CD40 in an ELISA assay.
APPLICATION: ELISA
Functional Application: Stimulates the proliferation of mouse B cells and dendritic cells. The activity of CD40L increases 1’000-fold (stimulation in the ng/ml range) in the presence of the cross-linking enhancer (Prod. No. ALX-804-034).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
The persistent elimination of B cells responding to blood group A carbohydrates by synthetic group A carbohydrates and B-1 cell differentiation blockade: novel concept in preventing antibody-mediated rejection in ABO-incompatible transplantation: T. Irei, et al.; Blood 110, 4567 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION CD40L (Prod. No. ALX-522-070) and Enhancer for Ligands (Prod. No. ALX-804-034) should be incubated together for 30 min. at room temperature prior to cell application. Effects are typically best seen with 0.5-10µg/ml of CD40L together with 1-2µg/ml of enhancer. This may vary by cell type and experimental conditions.

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Receptor/Ligand + Enhancer Set • CD Kits & Sets