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Adaptor Proteins & Interaction Domains/Related Products
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ALX-350-014 Revised 03-Apr-08
Calyculin A
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Other Signal Transduction Pathway Modulators
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-014-C010   10 µg 65.00 USD Add To Cart
ALX-350-014-C025   25 µg 110.00 USD Add To Cart
ALX-350-014-C050   50 µg 190.00 USD Add To Cart
ALX-350-014-C100   100 µg 320.00 USD Add To Cart
Product Specification
FORMULA: C50H81N4O15P
MW: 1009.2
CAS NUMBER: 101932-71-2
SOURCE/HOST: Isolated from Discodermia calyx.
PURITY: ≥95%
APPEARANCE: Lyophilized.
SOLUBILITY: Soluble in DMSO, 100% ethanol, methanol, acetonitrile, chloroform, methylene chloride or benzene. Insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: May by subject to isomerization when in solution. Biological activity is not affected by isomerization.
HANDLING: Protect from light and moisture.
HAZARD: TOXIC. MAY BE CARCINOGENIC.

Product Description
Potent cell permeable inhibitor with high specificity for protein phosphatase 1 (PP1) and 2A (PP2A). Inhibits p130cas tyrosine phosphorylation. Enhances the phosphorylation level of NF-κB. Affects intracellular signalling processes that require 14-3-3. Potent non-phorbol type tumor promoter. Prevents γ-radiation induced apoptosis.
Product Specific Literature References
Okadaic acid: an additional non-phorbol-12-tetradecanoate-13-acetate- type tumor promoter: M. Suganuma, et al.; PNAS 85, 1768 (1988) Abstract
Protein phosphatases come of age: P.Cohen & P.T. Cohen; J. Biol. Chem. 264, 21435 (1989) Abstract; Full Text
Calyculin A and okadaic acid: inhibitors of protein phosphatase activity: H. Ishihara, et al.; BBRC 159, 871 (1989) Abstract
The structure and regulation of protein phosphatases: P. Cohen; Ann. Rev. Biochem. 58, 453 (1989) Abstract
Calyculin A, an inhibitor of protein phosphatases, a potent tumor promoter on CD-1 mouse skin: M. Suganuma, et al.; Cancer Res. 50, 3521 (1990) Abstract
Nonphorbol tumor promoters okadaic acid and calyculin-A induce membrane translocation of protein kinase C: R. Gopalakrishna; BBRC 189, 950 (1992) Abstract
Comparative effects of protein phosphatase inhibitors (okadaic acid and calyculin A) on human leukemia HL60, HL60/ADR and K562 cells: K. Sakurada, et al.; BBRC 187, 488 (1992) Abstract
Calyculin A, a potent inhibitor of phosphatases-1 and -2A, prevents apoptosis: Q. Song & M.F. Lavin; BBRC 190, 47 (1993) Abstract
Enhancement of AMPA-mediated synaptic transmission by the protein phosphatase inhibitor calyculin A in rat hippocampal slices: A. Figurov, et al.; Eur. J. Neurosci. 5, 1035 (1993) Abstract
Calyculin A, a non-phorbol ester type tumor promotor, induced oxidative DNA damage in stimulated human neutrophil-like cells: T. Takeuchi, et al.; BBRC 205, 1803 (1994) Abstract
Potassium and calcium channel involvement in induction of long-lasting synaptic enhancement by calyculin A, a protein phosphatase inhibitor, in rat hippocampal CA1 region: N. Murakami, et al.; Neurosci. Lett. 176, 181 (1994) Abstract
Differential toxicity of the protein phosphatase inhibitors microcystin and calyculin A: M.T. Runnegar, et al.; J. Pharmacol. Exp. Ther. 273, 545 (1995) Abstract
Inhibition of p130cas tyrosine phosphorylation by calyculin A: W. Qiu, et al.; J. Leukoc. Biol. 63, 631 (1998) Abstract
Unique features of the okadaic acid activity class of tumor promoters: H. Fujiki & M. Suganuma; J. Cancer Res. Clin. Oncol. 125, 150 (1999), Review Abstract
Calyculin A-induced vimentin phosphorylation sequesters 14-3-3 and displaces other 14-3-3 partners in vivo: G. Tzivion, et al.; J. Biol. Chem. 275, 29772 (2000) Abstract
Calyculin A induces apoptosis and stimulates phosphorylation of p65NF-kappaB in human osteoblastic osteosarcoma MG63 cells: H. Tanaka, et al.; Int. J. Oncol. 31, 389 (2007) Abstract
Further Categories Containing This Product:
PP2A/Related Products • PP1/Related Products • Natural Products - Other Tumor Promoters • 14-3-3 Proteins/Related Products • Carcinogens & Tumor Promoters Other Products • NF-kB Pathway Modulators • Natural Products - Apoptosis Inducers & Inhibitors
 
 
ALX-350-115 Revised 21-Feb-08
Fusicoccin
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Plant Research Reagents
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ALX-350-115-MC05   0.5 mg 70.00 USD Add To Cart
ALX-350-115-M001   1 mg 120.00 USD Add To Cart
Product Specification
FORMULA: C36H56O12
MW: 680.8
CAS NUMBER: 20108-30-9
SOURCE/HOST: Isolated from fungus Fusicoccum amygdali.
PURITY: ≥90% (HPLC)
APPEARANCE: White to off-white crystalline solid.
SOLUBILITY: Soluble in acetone, 100% ethanol, methanol, chloroform or ethyl acetate; practically insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: TOXIC.

Product Description
Diterpene glucoside with numerous effects on plants. Induces H+ extrusion from plant cells by 14-3-3-dependent activation of the plasma membrane H+-ATPase. Stimulates cell enlargement, ion uptake, seed germination, opening the stomata, etc. Markedly reduces the cell’s capability for H2O2 scavenging.
Product Specific Literature References
Fusicoccin - a key to multiple 14-3-3 locks?: A.H. De Boer; Trends Plant Sci. 2, 20 (1997)
Fusicoccin binding to its plasma membrane receptor and the activation of the plasma membrane H(+)-ATPase. IV. Fusicoccin induces the association between the plasma membrane H(+)-ATPase and the fusicoccin receptor: C. Olivari, et al.; Plant Physiol. 116, 529 (1998) Abstract
Cytoskeletal inhibitors suppress the stomatal opening of Vicia faba L. induced by fusicoccin and IAA: R.F. Huang, et al.; Plant Sci. 156, 65 (2000) Abstract
Inhibition of catalase activity as an early response of Arabidopsis thaliana cultured cells to the phytotoxin fusicoccin: N. Beffagna & I. Lutzu; J. Exp. Bot. 58, 4183 (2007) Abstract
General Literature References
Fusicoccin: a tool in plant physiology: E. Marre; Ann. Rev. Plant Physiol. 30, 273 (1979), (Review)
Fusicoccin receptors: perception and transduction of the fusicoccin signal: P. Aducci et al.; J. Exp. Bot. 46, 1463 (1995), (Review)
Fusicoccin - a key to multiple 14-3-3 locks?: A.H. De Boer; Trends Plant Sci. 2, 20 (1997), (Review)
Fusicoccin binding to its plasma membrane receptor and the activation of the plasma membrane H(+)-ATPase. IV. Fusicoccin induces the association between the plasma membrane H(+)-ATPase and the fusicoccin receptor: C. Olivari, et al.; Plant Physiol. 116, 529 (1998) Abstract
Phosphorylation-independent interaction between 14-3-3 protein and the plant plasma membrane H+-ATPase: J. Borch, et al.; Biochem. Soc. Trans. 30, 411 (2002), (Review) Abstract; Full Text
Plant 14-3-3 proteins assist ion channels and pumps: A.H. de Boer; Biochem. Soc. Trans. 30, 416 (2002), (Review) Abstract; Full Text
Metabolic enzymes as targets for 14-3-3 proteins: S.C. Huber, et al.; Plant Mol. Biol. 50, 1053 (2002), (Review) Abstract
cDNA microarray analysis of fusicoccin-induced changes in gene expression in tomato plants: U.B. Frick & A. Schaller; Planta 216, 83 (2002) Abstract
Mutational analysis of the interaction between 14-3-3 proteins and plant plasma membrane H+-ATPase: S. Visconti, et al.; J. Biol. Chem. 278, 8172 (2003) Abstract
Structural view of a fungal toxin acting on a 14-3-3 regulatory complex: M. Wurtele, et al.; Embo J. 22, 987 (2003) Abstract
Further Categories Containing This Product:
H+-ATPase/Related Products • 14-3-3 Proteins/Related Products • Plant Research Reagents/Related Products
 
 
ALX-165-005 Revised 16-Feb-05
PDGF β-Receptor (719-723) (phosphorylated)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY SH2 & SH3 Domains
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-165-005-M001   1 mg 130.00 USD Add To Cart
ALX-165-005-M005   5 mg 520.00 USD Add To Cart
Product Specification
SEQUENCE: H-Tyr(PO3H2)-Val-Pro-Met-Leu-OH
FORMULA: C30H48N5O10PS
MW: 701.8
PURITY: ≥97%
APPEARANCE: White lyophilized powder.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Contains the PDGF β-receptor recognition sequence which specifically interacts with the SH2 domain of PI(3)K (phosphoinositide 3-kinase). In this phosphorylated form the peptide inhibits the binding of the 85kDa subunit of PI(3)K to the PDGF β-receptor.
Product Specific Literature References
Distinct phosphotyrosines on a growth factor receptor bind to specific molecules that mediate different signaling pathways: W.J. Fantl, et al.; Cell 69, 413 (1992) Abstract
Closing in on SH2 specificity: R.B. Birge & H. Hanafusa; Science 262, 1522 (1993) Abstract
Further Categories Containing This Product:
Peptides • Platelet-derived Growth Factors [PDGFs] & Receptors/Related Products
 
 
ALX-165-019 Revised 24-Feb-05
PDGF Fragment (phosphorylated) (cyclic)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY SH2 & SH3 Domains
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ALX-165-019-MC01   0.1 mg 50.00 USD Add To Cart
ALX-165-019-M001   1 mg 250.00 USD Add To Cart
Product Specification
SEQUENCE: cyclo(Gly-Tyr(PO3H2)-Val-Pro-Met-Leu)
FORMULA: C32H49N6O10PS
MW: 740.8
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Cyclic hexapeptide inhibitor of SH2 interactions. The peptide sequence represents the autophosphorylation region around Tyr751 of the PDGF β-receptor.
Product Specific Literature References
Synthesis of phosphonomethyl-phenylalanine and phosphotryrosine containing cyclic peptides as inhibitors of protein kinase/SN2 interactions: M. Nomizu, et al.; Tetrahedron 50, 2691 (1994)
Further Categories Containing This Product:
Peptides • Platelet-derived Growth Factors [PDGFs] & Receptors/Related Products
 
 
ALX-168-003 Revised 02-Apr-03
Tyrosine Phosphorylation Site Inhibitor
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Tyrosine Kinase Inhibitors
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ALX-168-003-M001   1 mg 60.00 USD Add To Cart
ALX-168-003-M005   5 mg 240.00 USD Add To Cart
Product Specification
SEQUENCE: H-Gly-Ser-Phe-Leu-Val-Arg-Glu-Ser-OH
FORMULA: C39H63N11O13
MW: 894.0
PURITY: ≥97%
APPEARANCE: White lyophilized powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Inhibitor of EGF receptor binding to SH2. The peptide corresponds to a highly conserved amino acid sequence common in a variety of SH2-containing proteins. It is the minimum essential sequence for recognition of the phosphotyrosine site.
Product Specific Literature References
Highly conserved eight amino acid sequence in SH2 is important for recognition of phosphotyrosine site: M. Hidaka, et al.; BBRC 180, 1490 (1991) Abstract
Further Categories Containing This Product:
Peptides • SH2 & SH3 Domains
 
 
ALX-153-064 Revised 31-Aug-07
Caveolin-1 Scaffolding Domain Peptide
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SYNONYMS Cavtratin
AP-Cav
Pen-C1-SD
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Caveolae/Related Products
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ALX-153-064-M001   1 mg 140.00 USD Add To Cart
ALX-153-064-M005   5 mg 560.00 USD Add To Cart
Product Specification
SEQUENCE: H-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Asp-Gly-Ile-Trp-Lys-Ala-Ser-Phe-Thr-Thr-Phe-Thr-Val-Thr-Lys-Tyr-Trp-Phe-Tyr-Arg-OH
FORMULA: C228H335N61O49S
MW: 4746.6
APPEARANCE: ≥97% (HPLC)
SOLUBILITY: Soluble in DMSO or acentonitrile:water (50:50).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Stock solutions are stable for up to 3 months when stored at -20°C.
HANDLING: Hygroscopic. Protect from light.
HAZARD: MAY BE CARCINOGENIC. HARMFUL.
Product Description
Cell permeable peptide that blocks eNOS (NOS III) activity and cellular nitric oxide (NO) release in vitro and reduces inflammation and tumorigenesis in vivo. Caveolin-1 interacts with several lipid-modified signalling ligands, such as EGFR, eNOS, G-protein α-subunits, PKCα, H-Ras, and Src, via the C1-SD82-101 sequence.
Product Specific Literature References
Caveolins, a family of scaffolding proteins for organizing "preassembled signaling complexes" at the plasma membrane: T. Okamoto, et al.; J. Biol. Chem. 273, 4519 (1998) Abstract; Full Text
In vivo delivery of the caveolin-1 scaffolding domain inhibits nitric oxide synthesis and reduces inflammation: M. Bucci, et al.; Nat. Med. 6, 1362 (2000) Abstract; Full Text
Selective inhibition of tumor microvascular permeability by cavtratin blocks tumor progression in mice: J.P. Gratton, et al.; Cancer Cell 4, 31 (2003) Abstract; Full Text
Caveolin-1 gene disruption promotes mammary tumorigenesis and dramatically enhances lung metastasis in vivo. Role of Cav-1 in cell invasiveness and matrix metalloproteinase (MMP-2/9) secretion: T.M. Williams, et al.; J. Biol. Chem. 279, 51630 (2004) Abstract; Full Text
Dissecting the molecular control of endothelial NO synthase by caveolin-1 using cell-permeable peptides: P.N. Bernatchez, et al.; PNAS 102, 761 (2005) Abstract; Full Text
Further Categories Containing This Product:
NOS Inhibitors (NOS Induction & Enzyme Activity) • NOS Regulation • Cancer Other Products • Peptides
 
 
ALX-748-017 Revised 24-Mar-05
FLIP (human) cDNA Probe
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FLIP
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ALX-748-017-C001   1 µg 140.00 USD Add To Cart
Product Specification
SOURCE/HOST: Derived from the coding region of the human FLIPL gene. Has a length of ~700 bp located within the region between nucleotides 1-700.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (agarose gel electrophoresis)
FORMULATION: Lyophilized powder.
RECONSTITUTION: Reconstitute with 10µl sterile water or 10mM Tris.HCl containing 0.1mM Na2EDTA.
APPLICATION: Northern Blot. Human FLIP mRNA migrates as a 4.7kb, 3.9kb and 2.2kb species.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
DNA & RNA Probes/Related Products
 
 
ALX-840-601 Revised 06-Dec-07
Positive Control (Cell Lysate) for Antibodies to FLIP (human)
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SYNONYMS Positive Control (Cell Lysate) for Antibodies to CASPER (human)
Positive Control (Cell Lysate) for Antibodies to CLARP (human)
Positive Control (Cell Lysate) for Antibodies to I-FLICE (human)
Positive Control (Cell Lysate) for Antibodies to Usurpin (human)
PRODUCT LINE Immunology
PRODUCT CATEGORY Positive Controls (Cell Lysates & Extracts)
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ALX-840-601-R100   100 µl 70.00 USD Add To Cart
Product Specification
SOURCE/HOST: Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human FLIP (aa 1-480) fused at the C-terminus to a HA-tag.
QUANTITY: 100µl corresponding to 2x106 cells.
FORMULATION: Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION: Positive control in Western blot for MAb to FLIP (Dave-2) (Prod. No. ALX-804-127) and MAb to FLIP (human) (NF6) (Prod. No. ALX-804-428).
Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
FLIP
 
 
ALX-522-085 Revised 21-Dec-06
RAIDD (human) (recombinant)
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SYNONYMS CRADD (human) (recombinant)
Caspase and RIP Adaptor with Death Domain (human) (recombinant)
RIP-associated Protein with a Death Domain (human) (recombinant)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY RAIDD/Related Products
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ALX-522-085-C050   50 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
MW: ~48kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. Full length human RAIDD (aa 1-199) is fused to a N-terminal tag.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
APPLICATION: Use as positive control for the PAb to RAIDD (human) (AT100) (Prod. No. ALX-210-915).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
RAIDD (RIP-associated ICH-1 homologous protein with a death domain) is an adaptor molecule that mediates the action of cysteine proteases involved in apoptosis. In human cell lines, the caspase-2 adaptor RAIDD interacts selectively with caspase-2 through its caspase recruitment domain (CARD) and leads to caspase-2-dependent death. RAIDD overexpression induced caspase-2, CARD- and caspase-9-dependent apoptosis of PC12 cells and sympathetic neurons. Apoptosis correlated with the formation of discrete perinuclear aggregates. Both death and aggregates required the expression of full-length RAIDD. Recently, it has been reported that activation of caspase-2 occurs in a complex that contains the death domain–containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress: A. Tinel and J. Tschopp; Science 304, 843 (2004) Abstract
RAIDD aggregation facilitates apoptotic death of PC12 cells and sympathetic neurons: O. Jabado, et al.; Cell Death Differ. 11, 618 (2004) Abstract
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-840-602 Revised 24-Nov-05
Positive Control (Cell Lysate) for Antibodies to Asc (human)
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SYNONYMS Positive Control (Cell Lysate) for Antibodies to Pycard (human)
PRODUCT LINE Immunology