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Kinases / Related Products
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ALX-270-249 Revised 11-Sep-08
Aminopurvalanol A
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SYNONYMS (2R)-2-((6-((3-Amino-5-chlorophenyl)amino)-9-(1-methylethyl)-
9H-purin-2-yl)amino)-3-methyl-1-butanol
NG-97
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY CDK & Cyclin Inhibitors
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ALX-270-249-M001   1 mg 45.00 USD Add To Cart
ALX-270-249-M005   5 mg 135.00 USD Add To Cart
Product Specification
FORMULA: C19H26N7OCl
MW: 403.9
CAS NUMBER: 220792-57-4
PURITY: ≥97% (1H-NMR)
APPEARANCE: White to brownish solid.
SOLUBILITY: Soluble in DMSO or methanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Packaged under inert gas. Protect from light.
HAZARD: IRRITANT.

Product Description
Cell permeable, reversible and competitive inhibitor of CDK1/cyclin B (IC50=33nM), CDK2/cyclin A (IC50=33nM), CDK2/cyclin E (IC50=28nM) and CDK5/p35 (IC50=20nM).
Product Specific Literature References
Synthesis and application of functionally diverse 2,6,9-trisubstituted purine libraries as CDK inhibitors: Y.T. Chang, et al.; Chem. Biol. 6, 361 (1999) Abstract
A cyclin-dependent kinase inhibitor inducing cancer cell differentiation: biochemical identification using Xenopus egg extracts: G.R. Rosania, et al.; PNAS 96, 4797 (1999) Abstract; Full Text
M-phase regulation of the recruitment of mRNAs onto polysomes using the CDK1/cyclin B inhibitor aminopurvalanol: M. Le Breton, et al.; BBRC 306, 880 (2003) Abstract
 
 
ALX-270-387 Revised 18-Jul-07
3-Amino-1H-pyrazolo[3,4-b]quinoxaline
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY CDK & Cyclin Inhibitors
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-270-387-M001   1 mg 40.00 USD Add To Cart
ALX-270-387-M005   5 mg 160.00 USD Add To Cart
Product Specification
FORMULA: C9H7N5
MW: 185.2
CAS NUMBER: 40254-90-8
PURITY: ≥98% (HPLC)
APPEARANCE: Red solid.
SOLUBILITY: Soluble in DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Protect from light. Packaged under inert gas.

Product Description
Selective inhibitor of CDK1/cyclin B (IC50=600nM), CDK5/p25 (IC50=400nM) and GSK-3β (IC50=1µM). Does not inhibit Cdc25 phosphatase activity (IC50>10µM).
Product Specific Literature References
Pyrazolo[3,4-b]quinoxalines. A new class of cyclin-dependent kinases inhibitors: M.A. Ortega, et al.; Bioorg. Med. Chem. 10, 2177 (2002) Abstract
Further Categories Containing This Product:
GSK-3 Inhibitors
 
 
ALX-202-027 Revised 09-Aug-06
cAMP-dependent Protein Kinase (bovine heart) (catalytic subunit)
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SYNONYMS Protein Kinase A (bovine heart) (catalytic subunit)
PKA (bovine heart) (catalytic subunit)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKA
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ALX-202-027-U500   500 U 250.00 USD Add To Cart
Product Specification
MW: ~40kDa.
EC: 2.7.1.37
SOURCE/HOST: Isolated from bovine heart.
CONCENTRATION: 20µg/ml.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 20mM MES, pH 6.5, containing 0.1mM EDTA, 30mM β-mercaptoethanol, 100mM sodium chloride and 50% ethylene glycol.
SPECIFIC ACTIVITY: ~1000 Units/µg using histone as the substrate. One unit is defined as the amount of enzyme that transfers 1pmol phosphate to histone per min. at 30°C.
APPLICATION: For studying PKA phosphorylation in vitro.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Do not freeze/thaw.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link: cAMP-dependent Protein Kinase (bovine) α catalytic subunit (P00517), β-1 catalytic subunit (P05131), β-2 catalytic subunit (P24256)
AfCS Signalling Gateway link: cAMP-dependent Protein Kinase (mouse) α catalytic subunit (A001914), β catalytic subunit (A001815)γ catalytic subunit (A001916)
Further Categories Containing This Product:
EnzymesNatural Proteins
 
 
ALX-151-016 Revised 17-Jan-05
cAMP-dependent Protein Kinase Inhibitor (5-22) amide
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SYNONYMS PKA Inhibitor (5-22) amide
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKA Inhibitors
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ALX-151-016-MC05   0.5 mg 110.00 USD Add To Cart
ALX-151-016-M001   1 mg 180.00 USD Add To Cart
Product Specification
SEQUENCE: H-Thr-Thr-Tyr-Ala-Asp-Phe-Ile-Ala-Ser-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile-NH2
FORMULA: C84H137N29O26
MW: 1969.2
PURITY: ≥97%
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Potent inhibitor of cAMP-dependent protein kinase (PKA).
Product Specific Literature References
A potent synthetic peptide inhibitor of the cAMP-dependent protein kinase: H.-C. Cheng, et al.; J. Biol. Chem. 261, 989 (1986) Abstract; Full Text
Primary structural determinants essential for potent inhibition of cAMP- dependent protein kinase by inhibitory peptides corresponding to the active portion of the heat-stable inhibitor protein: D.B. Glass, et al.; J. Biol. Chem. 264, 8802 (1989) Abstract; Full Text
Further Categories Containing This Product:
Peptides
 
 
ALX-151-014 Revised 15-Dec-04
cAMP-dependent Protein Kinase Inhibitor (5-24)
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SYNONYMS PKA Inhibitor (5-24)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKA Inhibitors
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ALX-151-014-MC05   0.5 mg 90.00 USD Add To Cart
ALX-151-014-M001   1 mg 160.00 USD Add To Cart
Product Specification
SEQUENCE: H-Thr-Thr-Tyr-Ala-Asp-Phe-Ile-Ala-Ser-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile-His-Asp-OH
FORMULA: C94H148N32O31
MW: 2222.4
PURITY: ≥97%
APPEARANCE: White to off-white powder.
FORMULATION: Lyophilized.
SOLUBILITY: Soluble in water or 5 % acetic acid.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Keep cool and dry.
Product Description
Potent inhibitor of cAMP-dependent protein kinase (PKA). The peptide corresponds to the active site of the skeletal muscle inhibitor protein.
Product Specific Literature References
A potent synthetic peptide inhibitor of the cAMP-dependent protein kinase: H.-C. Cheng, et al.; J. Biol. Chem. 261, 989 (1986) Abstract; Full Text
Phosphorylation of Avena phytochrome in vitro as a probe of light- induced conformational changes: Y.S. Wong, et al.; J. Biol. Chem. 261, 12089 (1986) Abstract; Full Text
Further Categories Containing This Product:
Peptides
 
 
ALX-151-018 Revised 13-Feb-06
cAMP-dependent Protein Kinase Inhibitor (5-24) amide
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SYNONYMS PKA Inhibitor (5-24) amide
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKA Inhibitors
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ALX-151-018-MC05   0.5 mg 90.00 USD Add To Cart
ALX-151-018-M001   1 mg 160.00 USD Add To Cart
Product Specification
SEQUENCE: H-Thr-Thr-Tyr-Ala-Asp-Phe-Ile-Ala-Ser-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile-His-Asp-NH2
FORMULA: C94H149N33O30
MW: 2221.4
PURITY: ≥97%
APPEARANCE: White to off-white lyophilized powder.
SOLUBILITY: Soluble in water or DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Specific Literature References
A potent synthetic peptide inhibitor of the cAMP-dependent protein kinase: H.-C. Cheng, et al.; J. Biol. Chem. 261, 989 (1986) Abstract; Full Text
Further Categories Containing This Product:
Peptides
 
 
ALX-151-017 Revised 15-Dec-04
cAMP-dependent Protein Kinase Inhibitor (14-24) amide
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SYNONYMS PKA Inhibitor (14-24) amide
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKA Inhibitors
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ALX-151-017-M001   1 mg 90.00 USD Add To Cart
ALX-151-017-M005   5 mg 360.00 USD Add To Cart
Product Specification
SEQUENCE: H-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile-His-Asp-NH2
FORMULA: C49H86N24O15
MW: 1251.4
PURITY: ≥97%
APPEARANCE: White powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Peptides
 
 
ALX-151-019 Revised 15-Dec-04
cAMP-dependent Protein Kinase Substrate
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SYNONYMS PKA Substrate
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKA Substrates
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ALX-151-019-MC05   0.5 mg 95.00 USD Add To Cart
ALX-151-019-M001   1 mg 165.00 USD Add To Cart
Product Specification
SEQUENCE: H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser-Val-Ala-Ala-Glu-OH
FORMULA: C92H150N28O29
MW: 2112.4
PURITY: ≥95%
APPEARANCE: White powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Derived from the RII subunit of cAMP-dependent protein kinase (PKA). Substrate for PKA. Has been used in a non-radioactive assay of calcineurin (protein phosphatase 2B) for screening purposes.
Product Specific Literature References
Dephosphorylation of cAMP-dependent protein kinase regulatory subunit (type II) by calmodulin-dependent protein phosphatase. Determinants of substrate specificity: D.K. Blumenthal, et al.; J. Biol. Chem. 261, 8140 (1986) Abstract; Full Text
Nonradioactive assay for protein phosphatase 2B (calcineurin) activity using a partial sequence of the subunit of cAMP-dependent protein kinase as substrate: A. Enz, et al.; Anal. Biochem. 216, 147 (1994) Abstract
Further Categories Containing This Product:
PP2B [Calcineurin] / Related ProductsPeptides
 
 
ALX-340-029 Revised 08-Jul-08
Anandamide
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SYNONYMS AEA
N-Arachidonoylethanolamine
(all-Z)-N-(2-Hydroxyethyl)-5,8,11,14-eicosatetraenamide
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Anandamide & Anandamide Analogs
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ALX-340-029-M005   5 mg 51.00 USD Add To Cart
Product Specification
FORMULA: C22H37NO2
MW: 347.5
CAS NUMBER: 94421-68-8
MERCK INDEX: 14: 624
RTECS: JX3842500
CONCENTRATION: 50mg/ml 
PURITY: ≥98%
FORMULATION: Liquid. Solution in ethanol.
SOLUBILITY: 10mg/ml soluble in DMSO or dimethyl formamide.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 6 months in other organic solvents when stored at -20°C.
HANDLING: Protect from light and oxygen.

Product Description
Endogenous [1] ligand for the CB1 receptor (CB1: Ki=52nm; CB2: Ki=1930nm [2]) and TRPV1 (Ki=5.78µM [3,4]). Inhibits NF-κB activation through direct binding to IKKβ [5] and induces apoptosis independently of cannabinoid or vanilloid receptors [6]. Activates the MAP kinase (MAPK/ERK) signalling pathway [7].
Product Specific Literature References
[1] Isolation and structure of a brain constituent that binds to the cannabinoid receptor: W.A. Devane, et al.; Science 258, 1946 (1992) Abstract
[2] Novel analogues of arachidonylethanolamide (anandamide): affinities for the CB1 and CB2 cannabinoid receptors and metabolic stability: S. Lin, et al.; J. Med. Chem. 41, 5353 (1998) Abstract
[3] Vanilloid receptors on sensory nerves mediate the vasodilator action of anandamide: P.M. Zygmunt, et al.; Nature 400, 452 (1999) Abstract
[4] Structure-activity relationship for the endogenous cannabinoid, anandamide, and certain of its analogues at vanilloid receptors in transfected cells and vas deferens: R.A. Ross, et al.; Br. J. Pharmacol. 132, 631 (2001) Abstract
[5] Anandamide inhibits nuclear factor-kappaB activation through a cannabinoid receptor-independent pathway: R. Sancho, et al.; Mol. Pharmacol. 63, 429 (2003) Abstract
[6] Anandamide induces cell death independently of cannabinoid receptors or vanilloid receptor 1: possible involvement of lipid rafts: K.P. Sarker, et al.; Cell Mol. Life Sci. 60, 1200 (2003) Abstract
[7] The MAP kinase signal transduction pathway is activated by the endogenous cannabinoid anandamide: M. Wartmann, et al.; FEBS Lett. 359, 133 (1995) Abstract
Biochemistry and pharmacology of arachidonylethanolamide, a putative endogenous cannabinoid: C.J. Hillard & W.B. Campbell; J. Lipid Res. 38, 2383 (1997), (Review) Abstract
The endogenous cannabinoid anandamide inhibits human breast cancer cell proliferation: L. De Petrocellis, et al.; PNAS 95, 8375 (1998) Abstract
Cannabinoid receptors and their endogenous agonist, anandamide: J. Axelrod & C.C. Felder; Neurochem. Res. 23, 575 (1998), (Review) Abstract
Endocannabinoids: endogenous cannabinoid receptor ligands with neuromodulatory action: V. Di Marzo, et al.; TINS 21, 521 (1998), (Review) Abstract
Endocannabinoids: a new class of vasoactive substances: M.D. Randall & D.A. Kendall; TIPS 19, 55 (1998), (Review) Abstract
Metabolism of anandamide and 2-arachidonoylglycerol: an historical overview and some recent developments: V. Di Marzo, et al.; Lipids 34, S319 (1999), (Review) Abstract
Anandamide: a candidate neurotransmitter heads for the big leagues: D.W. Self; Nat. Neurosci. 2, 303 (1999), (Review) Abstract
New perspectives on enigmatic vanilloid receptors: A. Szallasi & V. Di Marzo; TINS 23, 491 (2000), (Review) Abstract
Anandamide: some like it hot: V. Di Marzo, et al.; TIPS 22, 346 (2001), (Review) Abstract
Anandamide is an endogenous inhibitor for the migration of tumor cells and T lymphocytes: J. Joseph, et al.; Cancer Immunol. Immunother. 53, 723 (2004) Abstract
Anandamide-induced cell death in primary neuronal cultures: role of calpain and caspase pathways: V.A. Movsesyan, et al.; Cell Death Differ. 11, 1121 (2004) Abstract
Arachidonylethanolamide induces apoptosis of human glioma cells through vanilloid receptor-1: E. Contassot, et al.; J. Neuropathol. Exp. Neurol. 63, 956 (2004) Abstract
Preferred conformations of endogenous cannabinoid ligand anandamide: J.Z. Chen, et al.; Life Sci. 76, 2053 (2005) Abstract
The cardiovascular actions of anandamide: more targets?: M.D. Randall; Br. J. Pharmacol. 145, 565 (2005) Abstract; Full Text
Accumulation of anandamide: Evidence for cellular diversity: C.J. Hillard and A. Jarrahian; Neuropharmacology 48, 1072 (2005) Abstract
Anandamide as an intracellular messenger regulating ion channel activity: M. van der Stelt and V. Di Marzo; Prostaglandins Other Lipid Mediat. 77, 111 (2005) Abstract
Anandamide reduces infarct size in rat isolated hearts subjected to ischaemia-reperfusion by a novel cannabinoid mechanism: N.J. Underdown, et al.; Br. J. Pharmacol. 146, 809 (2005) Abstract
Anandamide inhibits adhesion and migration of breast cancer cells: C. Grimaldi, et al.; Exp. Cell Res. 312, 363 (2006) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION To change the solvent, evaporate the ethanol under a gentle stream of nitrogen and immediately add the solvent of choice.
 
 
ALX-380-051 Revised 20-Jun-08
Anisomycin
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SYNONYMS 2-(p-Methoxybenzyl)-3,4-pyrrolidinediol-3-acetate
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics - Other Signal Transduction Pathway Modulators
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-380-051-M010   10 mg 35.00 USD Add To Cart
ALX-380-051-M050   50 mg 140.00 USD Add To Cart
ALX-380-051-M100   100 mg 250.00 USD Add To Cart
Product Specification
FORMULA: C14H19NO4
MW: 265.3
CAS NUMBER: 22862-76-6
MERCK INDEX: 14: 670
RTECS: