ALEXIS Biochemicals: Rapamycin

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ALX-270-460

Revised 04-Sep-07

PI-103
SYNONYMS	3-(4-(4-Morpholinyl)pyrido[3’,2’:4,5]furo[3,2-d]pyrimidin-2-yl)phenol
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Phosphoinositide 3-kinase [PI(3)K] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-460-M001		1 mg	70.00 USD
ALX-270-460-M005		5 mg	280.00 USD
ALX-270-460-M025		25 mg	980.00 USD

Product Specification

FORMULA:	C₁₉H₁₆N₄O₃
MW:	348.4
CAS NUMBER:	371935-74-9
PURITY:	≥97% (HPLC)
APPEARANCE:	Off-white solid.
SOLUBILITY:	Soluble in DMSO (5mg/ml; warm).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions are stable for up to 3 months when stored at -20°C.
HANDLING:	Protect from light. After reconstitution, prepare aliquots and store at -20°C.

Product Description

Potent, cell permeable and ATP-competitive inhibitor of DNA-PK, phosphoinositide 3-kinase (PI(3)K), the rapamycin-sensitive (mTORC1) and rapamycin-insensitive (mTORC2) complexes of the protein kinase mTOR. Exhibits little activity towards a panel of more than 40 other kinases even at concentrations as high as 10µM. Effectively blocks cell proliferation in glioma cell lines both in vitro and in vivo.

Product Specific Literature References

A dual PI3 kinase/mTOR inhibitor reveals emergent efficacy in glioma: Q.W. Fan, et al.; Cancer Cell 9, 341 (2006) Abstract
A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signaling: Z.A. Knight, et al.; Cell 125, 733 (2006) Abstract

Further Categories Containing This Product:

Mammalian Target of Rapamycin [mTOR] / Related Products

ALX-210-142

Revised 12-Jun-06

Polyclonal Antibody to FKBP12
SYNONYMS	anti-FK506 Binding Protein (12kDa) PAb
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Immunophilins / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-142-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml.
PURITY DETAIL:	Epitope-affinity purified IgG.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 1-13 (G¹VQVETISPGDGR¹³) of human FKBP12 (FK506 binding protein 12kDa).
SPECIFICITY:	Recognizes human, mouse and rat FKBP12. Detects a band of ~12kDa by Western blot.
APPLICATION:	Immunohistochemistry (frozen sections (2µg/ml); paraffin sections) Immunoprecipitation Western Blot (1µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-156-002.

Product Images

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Product Specific Literature References

The effects of FK506 on retinal ganglion cells after optic nerve crush: E.E. Freeman and C.L. Grosskreutz; IOVS 41, 1111 (2000) Abstract
Immunophilin expression in the HIV-infected brain: C.L. Achim, et al.; J. Neuroimmunol. 157, 126 (2004) Abstract

Related Products

ALX-156-002

Blocking Peptide for PAb to FKBP12 (Prod. No. ALX-210-142)

Further Categories Containing This Product:

Mammalian Target of Rapamycin [mTOR] / Related Products • Polyclonal Antibodies

ALX-210-065

Revised 10-Dec-04

Polyclonal Antibody to mTOR
SYNONYMS	anti-Mammalian Target of Rapamycin PAb anti-FRAP1 PAb anti-RAFT1 PAb anti-RAPT1 PAb
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Mammalian Target of Rapamycin [mTOR] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-065-C050		50 µg	220.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Rat
SOURCE/HOST:	From rabbit.
CONCENTRATION:	12mg/ml
FORMULATION:	Liquid. Protein G purified antibody in PBS containing 10% glycerol and 0.02% sodium azide.
IMMUNOGEN:	Human mTOR (mammalian target of rapamycin) (aa 1223-1290).
SPECIFICITY:	Recognizes human and rat mTOR.
APPLICATION:	Immunoprecipitation (1-5µg) Western Blot (1µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Identification of TOR signaling complexes: more TORC for the cell growth engine: R.T. Abraham; Cell 111, 9 (2002) Abstract

Further Categories Containing This Product:

Polyclonal Antibodies

ALX-380-004

Revised 21-Feb-08

Rapamycin
SYNONYMS	Sirolimus AY-22,989 RAPA Rapamune
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antibiotics - Immunomodulators

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-380-004-C100		100 µg	35.00 USD
ALX-380-004-5100		5x100 µg	90.00 USD
ALX-380-004-M001		1 mg	120.00 USD
ALX-380-004-M005		5 mg	420.00 USD
ALX-380-004-M025		25 mg	950.00 USD

Product Specification

FORMULA:	C₅₁H₇₉NO₁₃
MW:	914.2
CAS NUMBER:	53123-88-9
MERCK INDEX:	14: 8114
RTECS:	VE6250000
SOURCE/HOST:	Isolated from Streptomyces hygroscopicus.
PURITY:	≥98% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in chloroform, DMSO or methanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HAZARD:	TOXIC.

Product Description

Macrocyclic-triene antibiotic possessing potent immunosuppressant activity. It forms a complex with FKBP12 that binds to an effector, thus inhibiting IL-2 and other growth promoting lymphokines. The effectors were recently identified as FRAP (FKBP12 rapamycin-associated protein) and RAFT1 (rapamycin and KFBP12 target). Rapamycin induces inhibition of p70^s6k, p33^cdk2 and p34^cdc2. Enhances apoptosis.

Product Specific Literature References

Rapamycin (AY-22,989), a new antifungal antibiotic. I. Taxonomy of the producing streptomycete and isolation of the active principle: C. Vezina, et al.; J. Antibiot. (Tokyo) 28, 721 (1975) Abstract
Rapamycin (AY-22,989), a new antifungal antibiotic. II. Fermentation, isolation and characterization: S.N. Sehgal, et al.; J. Antibiot. (Tokyo) 28, 727 (1975) Abstract
Rapamycin-induced inhibition of the 70-kilodalton S6 protein kinase: D.J. Price, et al.; Science 257, 973 (1992) Abstract
Rapamycin: in vitro profile of a new immunosuppressive macrolide: S.N. Sehgal & C.C. Bansback; Ann. N. Y. Acad. Sci. 685, 58 (1993) Abstract
Rapamycin inhibition of interleukin-2-dependent p33cdk2 and p34cdc2 kinase activation in T lymphocytes: W.G. Morice, et al.; J. Biol. Chem. 268, 22737 (1993) Abstract; Full Text
A mammalian protein targeted by G1-arresting rapamycin-receptor complex: E.J. Brown, et al.; Nature 369, 756 (1994) Abstract
RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin- dependent fashion and is homologous to yeast TORs: D.M. Sabatini, et al.; Cell 78, 35 (1994) Abstract
Rapamycin, a potent immunosuppressive drug, causes programmed cell death in B lymphoma cells: S. Muthukkumar, et al.; Transplantation 60, 264 (1995) Abstract
Rapamycin enhances apoptosis and increases sensitivity to cisplatin in vitro: Y. Shi, et al.; Cancer Res. 55, 1982 (1995) Abstract
Rapamycin potentiates dexamethasone-induced apoptosis and inhibits JNK activity in lymphoblastoid cells: T. Ishizuka, et al.; BBRC 230, 386 (1997) Abstract
Rapamycin and p53 act on different pathways to induce G1 arrest in mammalian cells: S.M. Metcalfe, et al.; Oncogene 15, 1635 (1997) Abstract
Rapamycin inhibition of the G1 to S transition is mediated by effects on cyclin D1 mRNA and protein stability: S. Hashemolhosseini, et al.; J. Biol. Chem. 273, 14424 (1998) Abstract; Full Text
Rapamycin causes poorly reversible inhibition of mTOR and induces p53- independent apoptosis in human rhabdomyosarcoma cells: H. Hosoi, et al.; Cancer Res. 59, 886 (1999) Abstract
Rapamycin: an anti-cancer immunosuppressant?: B.K. Law; Crit. Rev. Oncol. Hematol. 56, 47 (2005), Review Abstract
mTOR inhibitors (rapamycin and its derivatives) and nitrogen containing bisphosphonates: bi-functional compounds for the treatment of bone tumours: B. Ory, et al.; Curr. Med. Chem. 14, 1381 (2007), Review Abstract

Further Categories Containing This Product:

Mammalian Target of Rapamycin [mTOR] / Related Products • Antibiotics - Protein Kinase Inhibitors • Antibiotics - Antifungal

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