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Adaptor Proteins & Interaction Domains/Related Products
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APO-54N-038 Revised 27-May-08 New product
RIG-I (human) Detection Set (IntraCellular) [For ELISA Application]
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SYNONYMS Retinoic Acid-inducible Gene 1 (human) Detection Set (IntraCellular) [For ELISA Application]
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
APO-54N-038-KI01   1 Set 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 0.4ng/ml (range 0 to 32ng/ml).
QUANTITY: For 5x96 wells.
KIT/SET CONTAINS: 1 vial Standard (lyophilized) (STD).
1 vial Coating Antibody (COAT).
1 vial Detection Antibody (DET).
APPLICATION: For the quantitative determination of human RIG-I in cell extracts.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
The innate immune response to viruses is initiated upon detection of viral RNA generated during the life cycle of most viruses [1]. Recognition of the viral RNA is mediated by either the two cytoplasmic RNA helicases Retinoic acid Inducible Gene-1 (RIG-I ; Ddx58) and Melanoma Differentiation Associated gene 5 (MDA5 / Helicard) [2] or by Toll-like Receptor 3 (TLR3) located to an endosomal-like compartment [3]. RIG-I and MDA5 belong to the family of RIG-I like receptor (RLR) together with the protein LGP2 whose function is still elusive [4, 5]. Upon binding to RNA, RIG-I or MDA5 binds a new mediator named Cardif, MAVS, IPS-1 or VISA [6, 7] that signals through transcription factors, such as IRF-3, IRF-7, and NF-κB leading to the activation of interferons (IFNs) and other inflammatory cytokine genes. RIG-I is activated by dsRNA and 5′-ppp RNA. RIG-I has been shown to trigger innate immune signaling pathways during infection by orthomyxoviruses, rhabdovirus, vesicular stomatitis virus, and paramyxoviruses; and possibly by the filovirus Ebola virus and by the Epstein-Barr virus [8]. Virus infection induces a rapid production of IFN-α/β that leads to expression of hundreds of interferon-stimulated genes (ISGs) whose products direct antiviral actions. RIG-I belongs to the ISG gene family and monitoring its levels using the RIG-I (human) Detection Set (IC) can be used as indicator of IFN production and virus infection.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Recognition of viruses by innate immunity: O. Takeuchi & S. Akira; Immunol. Rev. 220, 214 (2007) Abstract
[2] MDA5/RIG-I and virus recognition: O. Takeuchi & S. Akira; Curr. Opin. Immunol. 20, 17 (2008) Abstract
[3] TLR3: Interferon induction by double-stranded RNA including poly(I:C): M. Matsumoto & T. Seya; Adv. Drug Deliv. Rev. 60, 805 (2008) Abstract
[4] Cytoplasmic recognition of RNA: M. Yoneyama, et al.; Adv. Drug Deliv. Rev. 60, 841 (2008) Abstract
[5] Loss of DExD/H box RNA helicase LGP2 manifests disparate antiviral responses: T. Venkataraman, et al.; J. Immunol. 178, 6444 (2007) Abstract; Full Text
[6] Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
[7] IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
[8] Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008) Abstract; Full Text
Related Products
Further Categories Containing This Product:
NF-kB Pathway Other Products • Antiviral Signalling/Related Products • Caspase Recruitment Domain [CARD] Proteins/Related Products • ELISA Sets • Interferons & Interferon Receptors Other Products
 
 
ALX-840-604 Revised 01-Mar-07
Positive Control (Cell Lysate) for Antibodies to RIP2 (human)
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SYNONYMS Positive Control (Cell Lysate) for Antibodies to Receptor-interacting Protein 2 (human)
Positive Control (Cell Lysate) for Antibodies to CARDIAK (human)
Positive Control (Cell Lysate) for Antibodies to CARD-containing IL-1β Converting Enzyme Associated Kinase (human)
Positive Control (Cell Lysate) for Antibodies to RICK (human)
Positive Control (Cell Lysate) for Antibodies to RIPK2 (human)
Positive Control (Cell Lysate) for Antibodies to Receptor-interacting Serine-Threonine Kinase 2 (human)
PRODUCT LINE Immunology
PRODUCT CATEGORY Positive Controls (Cell Lysates & Extracts)
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-840-604-R100   100 µl 70.00 USD Add To Cart
Product Specification
SOURCE/HOST: Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human RIP2 (receptor-interacting protein 2) (aa 1-541) fused at the C-terminus to a FLAG®-tag.
QUANTITY: 100µl corresponding to 2x106 cells.
FORMULATION: Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION: Positive control in Western blot for MAb to RIP2 (human) (Nick-1) (Prod. No. ALX-804-139). Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Nod-complex/Related Products • Caspase Recruitment Domain [CARD] Proteins/Related Products
 
 
ALX-168-003 Revised 02-Apr-03
Tyrosine Phosphorylation Site Inhibitor
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Tyrosine Kinase Inhibitors
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-168-003-M001   1 mg 60.00 USD Add To Cart
ALX-168-003-M005   5 mg 240.00 USD Add To Cart
Product Specification
SEQUENCE: H-Gly-Ser-Phe-Leu-Val-Arg-Glu-Ser-OH
FORMULA: C39H63N11O13
MW: 894.0
PURITY: ≥97%
APPEARANCE: White lyophilized powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Inhibitor of EGF receptor binding to SH2. The peptide corresponds to a highly conserved amino acid sequence common in a variety of SH2-containing proteins. It is the minimum essential sequence for recognition of the phosphotyrosine site.
Product Specific Literature References
Highly conserved eight amino acid sequence in SH2 is important for recognition of phosphotyrosine site: M. Hidaka, et al.; BBRC 180, 1490 (1991) Abstract
Further Categories Containing This Product:
Peptides • SH2 & SH3 Domains
 
 
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