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Caspase Recruitment Domain [CARD] Proteins/Related Products
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ALX-840-602 Revised 24-Nov-05
Positive Control (Cell Lysate) for Antibodies to Asc (human)
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SYNONYMS Positive Control (Cell Lysate) for Antibodies to Pycard (human)
PRODUCT LINE Immunology
PRODUCT CATEGORY Positive Controls (Cell Lysates & Extracts)
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-840-602-R100   100 µl 70.00 USD Add To Cart
Product Specification
SOURCE/HOST: Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human Asc (aa 1-195) fused at the C-terminus to a FLAG®-tag.
QUANTITY: 100µl corresponding to 2x105 cells.
FORMULATION: Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION: Positive control in Western blot for PAb to Asc (human) (AL177) (Prod. No. ALX-210-905). Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins/Related Products • Inflammasome
 
 
ALX-840-606 Revised 08-Mar-07
Positive Control (Cell Lysate) for Antibodies to CARMA1 (human)
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SYNONYMS Positive Control (Cell Lysate) for Antibodies to CARD-containing MAGUK Protein 1 (human)
Positive Control (Cell Lysate) for Antibodies to CARD11 (human)
Positive Control (Cell Lysate) for Antibodies to Caspase Recruitment Domain-containing Protein 11 (human)
Positive Control (Cell Lysate) for Antibodies to Bimp3 (human)
PRODUCT LINE Immunology
PRODUCT CATEGORY Positive Controls (Cell Lysates & Extracts)
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-840-606-R100   100 µl 70.00 USD Add To Cart
Product Specification
SOURCE/HOST: Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human CARMA1 (CARD-containing MAGUK protein 1) (aa 1-1147) fused at the C-terminus to a FLAG®-tag.
QUANTITY: 100µl corresponding to 2x105 cells.
FORMULATION: Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION: Positive control in Western blot for PAb to CARMA1 (AL220) (Prod. No. ALX-210-903). Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins/Related Products
 
 
APO-54N-035 Revised 20-Jun-08 New product
MDA5 (human) Detection Set (IntraCellular) [For ELISA Application]
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SYNONYMS Melanoma Differentiation-associated Gene 5 (human) Detection Set (IntraCellular) [For ELISA Application]
Helicard (human) Detection Set (IntraCellular) [For ELISA Application]
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases/Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
APO-54N-035-KI01   1 Set 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 0.4ng/ml (range 0 to 32ng/ml).
QUANTITY: For 5x96 wells.
KIT/SET CONTAINS: 1 vial Standard (lyophilized) (STD).
1 vial Coating Antibody (COAT).
1 vial Detection Antibody (DET).
APPLICATION: For the quantitative determination of human MDA5 in cell extracts.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Description

This intracellular Detection Set is for measuring protein levels in cell lysates.  It allows to quantitatively determine:
a. Levels of MDA5
b. Interferon response since MDA5 is an interferon inducible gene
b. Virus infection response
General Information
The innate immune response to viruses is initiated upon detection of viral RNA generated during the life cycle of most viruses [1]. Recognition of the viral RNA is mediated by either the two cytoplasmic RNA helicases Retinoic acid Inducible Gene-1 (RIG-I ; Ddx58) and Melanoma Differentiation Associated gene 5 (MDA5 / Helicard; Ifih1) [2] or by Toll-like Receptor 3 (TLR3) located to an endosomal-like compartment [3]. RIG-I and MDA5 belongs to the family of RIG-I like receptor (RLR) together with the protein LGP2 whose function is still elusive [4, 5]. Upon binding to RNA, RIG-I or MDA5 binds a new mediator named Cardif, MAVS (mitochondrial antiviral signalling), IPS-1 (interferon-β promoter stimulator 1) or VISA (virus-induced signalling adapter) [6, 7]. Cardif/ MAVS/ IPS-1/ VISA then interacts with several TRAFs molecules, leading to the activation of regulatory kinases, such as TBK1, IKKε , and the IKKα/β/γ complex. These kinases activate respective transcription factors, IRF-3, IRF-7, and NF-κB that participate in the activation of IFN and other inflammatory cytokine genes. RIG-I is activated by dsRNA and 5′-ppp RNA while only poly I: C acts as a chemical agonist for MDA5. The physiological agonist of MDA5 has still to be discovered. Mda-5 is an early response gene inducible by IFN and tumor necrosis factor-α, responding predominantly to IFN-β [8].
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9BYX4: MDA5 (human)
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[8] mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract; Full Text
[6] Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
[7] IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
[1] Recognition of viruses by innate immunity: O. Takeuchi & S. Akira; Immunol. Rev. 220, 214 (2007) Abstract
[4] Cytoplasmic recognition of RNA: M. Yoneyama, et al.; Adv. Drug Deliv. Rev. 60, 841 (2008) Abstract
[5] Loss of DExD/H box RNA helicase LGP2 manifests disparate antiviral responses: T. Venkataraman, et al.; J. Immunol. 178, 6444 (2007) Abstract
[2] MDA5/RIG-I and virus recognition: O. Takeuchi & S. Akira; Curr. Opin. Immunol. 20, 17 (2008) Abstract
[3] TLR3: Interferon induction by double-stranded RNA including poly(I:C): M. Matsumoto & T. Seya; Adv. Drug Deliv. Rev. 60, 805 (2008)
Further Categories Containing This Product:
NF-kB Pathway Other Products • Antiviral Signalling/Related Products • Caspase Recruitment Domain [CARD] Proteins/Related Products • Interferons & Interferon Receptors Other Products • ELISA Sets
 
 
ALX-804-349 Revised 18-Sep-07
Monoclonal Antibody to Apaf-1 (13F11)
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SYNONYMS anti-Apoptosis Protease-activating Factor 1 MAb (13F11)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Apaf-1
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ALX-804-349-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
CLONE: 13F11
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse Apaf-1 (apoptosis protease-activating factor 1) (aa 1-97) containing the N-terminal CARD domain.
SPECIFICITY: Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1.
APPLICATION: Immunocytochemistry
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
Decreased apoptosome activity with neuronal differentiation sets the threshold for strict IAP regulation of apoptosis: K.M. Wright, et al.; J. Cell Biol. 167, 303 (2004) Abstract; Full Text
General Literature References
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997) Abstract
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997) Abstract
Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development: F. Cecconi, et al.; Cell 94, 727 (1998) Abstract
Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation: Y. Hu, et al.; PNAS 95, 4386 (1998) Abstract; Full Text
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell Biol. 149, 623 (2000) Abstract; Full Text
Apaf1 in developmental apoptosis and cancer: how many ways to die?: F. Cecconi & P. Gruss; Cell. Mol. Life Sci. 58, 1688 (2001), (Review) Abstract
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins/Related Products • Monoclonal Antibodies
 
 
ALX-804-350 Revised 14-Aug-07
Monoclonal Antibody to Apaf-1 (18H2)
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SYNONYMS anti-Apoptosis Protease activating Factor 1 MAb (18H2)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Apaf-1
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ALX-804-350-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 18H2
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Protein G purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse Apaf-1 (apoptosis protease-activating factor-1) (aa 1-97) containing the N-terminal CARD domain.
SPECIFICITY: Recognizes the CARD domain of human and mouse Apaf-1. Does not cross-react with rat Apaf-1.
APPLICATION: ELISA
Immunocytochemistry
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell Biol. 149, 623 (2000) Abstract; Full Text
Apaf-1 and caspase-9 do not act as tumor suppressors in myc-induced lymphomagenesis or mouse embryo fibroblast transformation: C.L. Scott, et al.; J. Cell Biol. 164, 89 (2004) Abstract; Full Text
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death: V.S. Marsden, et al.; Blood 107, 1872 (2006) Abstract; Full Text
General Literature References
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997) Abstract
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997) Abstract
Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development: F. Cecconi, et al.; Cell 94, 727 (1998) Abstract
Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation: Y. Hu, et al.; PNAS 95, 4386 (1998) Abstract; Full Text
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell. Biol. 149, 623 (2000) Abstract; Full Text
Apaf1 in developmental apoptosis and cancer: how many ways to die?: F. Cecconi & P. Gruss; Cell. Mol. Life Sci. 58, 1688 (2001), (Review) Abstract
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins/Related Products • Monoclonal Antibodies
 
 
ALX-804-348 Revised 18-Sep-07
Monoclonal Antibody to Apaf-1 (human) (2E12)
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SYNONYMS anti-Apoptosis Protease-activating Factor 1 (human) MAb (2E12)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Apaf-1
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ALX-804-348-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 2E12
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human Apaf-1 (apoptosis protease-activating factor 1) N-terminal CARD and CED-4 homologous domains (aa 1-464) .
SPECIFICITY: Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.
APPLICATION: ELISA
Immunocytochemistry
Immunohistochemistry (EM on frozen sections; for details see G. Hausmann, et al., below.)
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
Bcl-2 family members do not inhibit apoptosis by binding the caspase activator Apaf-1: K. Moriishi, et al.; PNAS 96, 9683 (1999) Abstract; Full Text
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell Biol. 149, 623 (2000) Abstract; Full Text
Anti-Fas antibody-induced apoptosis and its signal transduction in human gastric carcinoma cell lines: K. Adachi, et al.; Int. J. Oncol. 23, 713 (2003) Abstract
Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein: D.H. Cho, et al.; J. Biol. Chem. 279, 39942 (2004) Abstract; Full Text
BOK and NOXA Are Essential Mediators of p53-dependent Apoptosis: A.G. Yakovlev, et al.; J. Biol. Chem. 279, 28367 (2004) Abstract
General Literature References
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997) Abstract
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997) Abstract
Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development: F. Cecconi, et al.; Cell 94, 727 (1998) Abstract
Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation: Y. Hu, et al.; PNAS 95, 4386 (1998) Abstract; Full Text
Apaf1 in developmental apoptosis and cancer: how many ways to die?: F. Cecconi & P. Gruss; Cell. Mol. Life Sci. 58, 1688 (2001), (Review) Abstract
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins/Related Products • Monoclonal Antibodies
 
 
ALX-804-134 Revised 01-Mar-07
Monoclonal Antibody to Bcl-10 (Carmen-1)
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SYNONYMS anti-CARMEN MAb (Carmen-1)
anti-CIPER MAb (Carmen-1)
anti-CLAP MAb (Carmen-1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Caspase Recruitment Domain [CARD] Proteins/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-134-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Carmen-1
ISOTYPE: Rat IgM
SOURCE/HOST: Purified from concentrated hybridoma cell culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: >95% (SDS-PAGE)
PURITY DETAIL: Purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human Bcl-10.
SPECIFICITY: Recognizes human and mouse Bcl-10.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY:

Stable for at least 6 months after receipt when stored undiluted at +4°C.
HANDLING: Do not freeze/thaw.
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
NF-kB Pathway Other Products • Innate Immunity Other Products • Monoclonal Antibodies
 
 
ALX-804-847 Revised 19-Jun-08
Monoclonal Antibody to Cardif (human) (Adri-1)
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SYNONYMS anti-CARD Adapter Inducing Interferon-β (human) MAb (Adri-1)
anti-IPS-1 Cardif (human) MAb (Adri-1)
anti-Interferon-β Promoter Stimulator Protein 1 (human) MAb (Adri-1)
anti-MAVS (human) MAb (Adri-1)
anti-Mitochondrial Antiviral Signalling Protein (human) MAb (Adri-1)
anti-VISA (human) MAb (Adri-1)
anti-Virus-induced Signalling Adapter (human) MAb (Adri-1)
PRODUCT LINE Immunology
PRODUCT CATEGORY Antiviral Signalling/Related Products
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ALX-804-847-C100   100 µg 370.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Adri-1
ISOTYPE: Mouse IgG2b
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).
SPECIFICITY: Recognizes human Cardif.
APPLICATION: Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections)
Immunoprecipitation (1:200)
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
TRADD Protein Is an Essential Component of the RIG-like Helicase Antiviral Pathway: M.C. Michallet, et al.; Immunity 28, 651 (2008) Abstract
General Information
RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons.
Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005) Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005) Abstract
CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006) Abstract
Further Categories Containing This Product:
NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins/Related Products • Polyclonal Antibodies
 
 
ALX-804-848 Revised 24-May-07
Monoclonal Antibody to IPAF (human) (Luna-1)
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SYNONYMS anti-Ice Protease-activating Factor (human) (Luna-1)
anti-CARD12 (human) (Luna-1)
anti-Caspase Recruitment Domain-containing Protein 12 (human) (Luna-1)
anti-CLAN (human) (Luna-1)
anti-CARD, LRR, and NACHT-containing Protein (human) (Luna-1)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Nod-complex/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-848-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Luna-1
ISOTYPE: Mouse IgG2b
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: