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Signal Transduction
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ALX-380-051 Revised 20-Jun-08
Anisomycin
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SYNONYMS 2-(p-Methoxybenzyl)-3,4-pyrrolidinediol-3-acetate
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics - Other Signal Transduction Pathway Modulators
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-380-051-M010   10 mg 35.00 USD Add To Cart
ALX-380-051-M050   50 mg 140.00 USD Add To Cart
ALX-380-051-M100   100 mg 250.00 USD Add To Cart
Product Specification
FORMULA: C14H19NO4
MW: 265.3
CAS NUMBER: 22862-76-6
MERCK INDEX: 14: 670
RTECS: BZ9800000
SOURCE/HOST: Isolated from Streptomyces griseolus.
PURITY: ≥98% (HPLC)
APPEARANCE: White crystalline solid.
SOLUBILITY: Soluble in DMSO (25mg/ml), 100% ethanol, methanol or ethyl acetate (10mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: TOXIC.

Product Description
Antibiotic. Activator of p38 and MAP kinases. Synergistic with growth factors and phorbol esters to superinduce cFos and cJun, by acting as a potent signalling agonist. Induces apoptosis in the human monoblastoid cell line. Used in the eradication of bean mildew. Inhibits other pathogenic fungi in plants.
Product Specific Literature References
Anisomycin, a new anti-protozoan antibiotic: B. A. Sobin & F. W. Tanner; J. Am. Chem. Soc. 76, 4053 (1954)
DNA fragmentation and cytolysis in U937 cells treated with diphtheria toxin or other inhibitors of protein synthesis: S.K. Kochi & R.J. Collier; Exp. Cell. Res. 208, 296 (1993) Abstract
The stress-activated protein kinase subfamily of c-Jun kinases: J.M. Kyriakis, et al.; Nature 369, 156 (1994) Abstract
Role of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-Jun: I. Sanchez, et al.; Nature 372, 794 (1994) Abstract
Anisomycin and rapamycin define an area upstream of p70/85S6k containing a bifurcation to histone H3-HMG-like protein phosphorylation and c-fos-c-jun induction: E. Kardalinou, et al.; Mol. Cell. Biol. 14, 1066 (1994) Abstract
Protein synthesis inhibitor phase shifts vasopressin rhythms in long- term suprachiasmatic cultures: K. Shinohara & T. Oka; NeuroReport 5, 2201 (1994) Abstract
Anisomycin selectively desensitizes signalling components involved in stress kinase activation and fos and jun induction: C.A. Hazzalin, et al.; Mol Cell Biol 18, 1844 (1998) Abstract
Anisomycin treatment paradigm affects duration of long-term potentiation in slices of the amygdala: P. Okulski, et al.; Neuroscience 114, 1 (2002) Abstract
Downregulation of Ski and SnoN co-repressors by anisomycin: A. Vazquez-Macias, et al.; FEBS Lett. 579, 3701 (2005) Abstract
Anisomycin and the reconsolidation hypothesis: J.W. Rudy, et al.; Learn. Mem. 13, 1 (2006), Review Abstract
A chemical screen identifies anisomycin as an anoikis sensitizer that functions by decreasing FLIP protein synthesis: I.A. Mawji, et al.; Cancer Res. 67, 8307 (2007) Abstract
Anisomycin abrogates repression of protooncogene c-fos transcription in E1A + cHa-ras-transformed cells through activation of MEK/ERK kinase cascade: A. N. Kukushkin, et al.; J. Cell. Biochem. 103, 1005 (2008) Abstract
Further Categories Containing This Product:
MAPK Pathway Activators • Antibiotics - Apoptosis Inducers & Inhibitors • JNK [SAPK1]/Related Products
 
 
ALX-201-116 Revised 21-Jan-08
Annexin V (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-116-C100   100 µg 194.00 USD Add To Cart
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Product Specification
MW: ~52kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. Recombinant human annexin V fused to GST.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Affinity purified.
FORMULATION: Lyophilized. Contains PBS, pH 7.4, and 0.02% sodium azide.
RECONSTITUTION: Reconstitute with 100µl sterile water.
SPECIFICITY: Binds to phosphatidylserine (PS).
BIOLOGICAL ACTIVITY: Exhibits anti-phospholipase activity.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Reconstituted solutions can be kept at 4°C under sterile conditions for one week or should be frozen in aliquots at -20°C. Repeated freeze/thaw cycles (>3) may result in an adverse effect on the activity of annexin V.
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-209-257 Revised 18-May-06
Annexin V (mouse) (recombinant) (Alexa Fluor® 488)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-209-257-T100   100 tests 245.00 USD Add To Cart
Product Specification
QUANTITY: 100 tests/500µl.
FORMULATION: Liquid. Contains 0.02% sodium azide.
SPECIFICITY: Binds phosphatidylserine (PS) residues.
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Protect from light. Do not freeze.
Product Images
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Product Description
Alexa Fluor® 488-labelled recombinant mouse annexin shows bright green fluorescence (Ex(max): 495nm; Em(max): 519nm).
Product Specific Literature References
[1] Engagement of the antigen-receptor on immature murine B lymphocytes results in death by apoptosis: A. Norvell, et al.; J. Immunol. 154, 4404 (1995) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION General Protocol: Detection of apoptotic cells by flow cytometry

- Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS).
- Resuspend cells in 100µl binding buffer (10mM HEPES, pH 7.4, 140mM sodium chloride, 2.5mM calcium dichloride), add 5µl annexin V-Alexa Fluor® 488 to the cells and mix gently.
  For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1µg/ml or 1.5µM).
- Incubate for 15 min. at room temperature in the dark.
- Add 400µl binding buffer and analyze the staining in flow cytometry as soon as possible (within an hour).

Swiss-Prot link P48036: Annexin V (mouse)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-209-252 Revised 05-Feb-08
Annexin V (human) (recombinant) (APC)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-209-252-T020   20 tests 106.00 USD Add To Cart
ALX-209-252-T100   100 tests 279.00 USD Add To Cart
Product Specification
MW: ~35.8kDa.
QUANTITY: 20 tests/100µl; 100 tests/500µl.
PURITY: ≥98% (SDS-PAGE, HPLC)
FORMULATION: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Do not freeze. Protect from light.
Product Description
APC-labelled recombinant human annexin V (produced in E. coli) is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable MAb (FITC-labelled or labelled secondary antibody). The fluorochrome APC (allophycocyanin) shows far red fluorescence (Ex(max): 650nm; Em(max): 660nm).
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:

 - Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2;
    filtered through 0.2µm pore filter);
 - Brief centrifugation before use is recommended
 - Wash cells in PBS by gentle shaking or pipetting up and down
 - resuspend cells in binding buffer; adjust cell density to 2-5x105/ml
 - Take 195µl cell suspension and add 5µl Annexin V-FITC
 - Mix and incubate 10 min. in the dark at room temperature
 - Wash cells 1x with PBS and resuspend in 190µl binding buffer
 - FACS analysis

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281 : Annexin V (mouse)
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-209-258 Revised 29-May-06
Annexin V (human) (recombinant) (ATTO 488)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-209-258-T100   100 tests 260.00 USD Add To Cart
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Product Specification
MW: ~52kDa (SDS-PAGE).
QUANTITY: 100 tests/0.5ml
PURITY: ≥90%
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry or immunofluorescence.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least one year after receipt when stored at +4°C.
HANDLING: Do not freeze. Protect from light.
Product Description
New ATTO-fluorescent dyes show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 488 shows bright green fluorescence (λabs (max): 501nm, λem (max): 523nm, εmax: 90'000).
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:

- Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm filter).
- Adjust cells to a density between 200’000 and 500’000 cells/ml.
- Take 195µl cell suspension and add 5µl Annexin-ATTO 488. Mix and incubate 10 min. in the dark.
- Wash cells 1x and resuspend in 190µl binding buffer.
- Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells.
- Analyze by FACS.
Note: These are general recommendations. Optimal conditions must be determined individually for each application:

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-209-259 Revised 11-Oct-06
Annexin V (human) (recombinant) (ATTO 647N)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-209-259-T100   100 tests 260.00 USD Add To Cart
Inquire
Product Specification
MW: ~52kDa (SDS-PAGE).
QUANTITY: 100 tests/0.5ml
PURITY: ≥90%
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry or immunofluorescence.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least one year after receipt when stored at +4°C.
HANDLING: Protect from light. Do not freeze.
Product Description
New ATTO-fluorescent dyes show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 647N shows red fluorescence (λabs (max): 645nm, λem (max): 669nm, εmax: 120’000).
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:

- Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm filter).
- Adjust cells to a density between 200’000 and 500’000 cells/ml.
- Take 195µl cell suspension and add 5µl Annexin-ATTO 647N. Mix and incubate 10 min. in the dark.
- Wash cells 1x and resuspend in 190µl binding buffer.
- Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells.
- Analyze by FACS.
Note: These are general recommendations. Optimal conditions must be determined individually for each application:

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-209-002 Revised 02-Aug-06
Annexin V (human) (recombinant) (Biotin)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-209-002-T020   20 tests 106.00 USD Add To Cart
ALX-209-002-T100   100 tests 352.00 USD Add To Cart
ALX-209-002-T300   300 tests 531.00 USD Add To Cart
Product Specification
MW: ~35.8kDa.
QUANTITY: 20 tests/100µl; 100 tests/500µl; 300 tests/1'500µl.
PURITY: ≥98% (SDS-PAGE, HPLC)
FORMULATION: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
[1] Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers: H.A. Andree, et al.; J. Biol. Chem. 265, 4923 (1990) Abstract; Full Text
[2] Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages: V.A. Fadok, et al.; J. Immunol. 148, 2207 (1992) Abstract
[3] Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis: G. Koopman, et al.; Blood 84, 1415 (1994) Abstract
[4] Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro: C.H. Homburg, et al.; Blood 85, 532 (1995) Abstract
[5] A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth. 184, 39 (1995) Abstract
[6] Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl: S. Martin, et al.; J. Exp. Med. 182, 1545 (1995) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[8] CD95 engagement releases calcium from intracellular stores of long term activated, apoptosis-prone gammadelta T cells: P. Rovere, et al.; J. Immunol. 156, 4631 (1996) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:
- Brief centrifugation before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down
- Resuspend cells in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered
through 0.2µm pore filter); adjust cell density to 2-5x105/ml
- Take 195µl cell suspension and add 5µl Annexin V-Biotin
- Mix and incubate 15 minutes at room temperature
- Wash cells 2x in binding buffer
- Resuspend in binding buffer
- Add Streptavidin-FITC
- Mix and incubate for 30 minutes in the dark at room temperature
- Wash cells 1x in binding buffer and resuspend in 190µl binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1mg/ml)
- FACS analysis

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281 : Annexin V (mouse)
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins • Biotinylated Reagents Other Products
 
 
ALX-209-256 Revised 18-May-06
Annexin V (human) (recombinant) (FITC)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-209-256-T100   100 tests 173.00 USD Add To Cart
ALX-209-256-3100   3x100 tests 366.00 USD Add To Cart
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Product Specification
MW: ~52kDa (SDS-PAGE).
QUANTITY: 100 tests/0.5ml; 3x100 tests/3x0.5ml.
PURITY: ≥90%
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least one year after receipt when stored at +4°C.
HANDLING: Do not freeze. Protect from light.
Product Images
Please click on thumbnails to enlarge.
Product Description
FITC-labelled recombinant human annexin V fusion protein (produced in E. coli) shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm).
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:

- Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm filter).
- Adjust cells to a density between 200'000 and 500'000 cells/ml.
- Take 195µl cell suspension and add 5µl Annexin-FITC. Mix and incubate 10 min. in the dark.
- Wash cells 1x and resuspend in 190µl binding buffer.
- Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells.
- Analyze by FACS.
Note: These are general recommendations. Optimal conditions must be determined individually for each application:

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products