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ALX-350-362 Revised 26-Mar-08
(+)-Egenine
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SYNONYMS 6-(6-Methyl-5,6,7,8-tetrahydro[1,3]dioxolo[4,5-g]isoquinolin-5-yl)-6,8-dihydrofuro[3,4-E][1,3]benzodioxol-8-ol
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Other Signal Transduction Pathway Modulators
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ALX-350-362-M025   25 mg 95.00 USD Add To Cart
ALX-350-362-M100   100 mg 285.00 USD Add To Cart
Product Specification
FORMULA: C20H19NO6
MW: 369.4
CAS NUMBER: 6883-44-9
SOURCE/HOST: Isolated from Fumaria vaillantii or Corydalis decumbens.
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white crystalline solid.
SOLUBILITY: Soluble in ethyl acetate or chloroform; sparingly soluble in 100% ethanol or ether; practically insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
First phthalideisoquinoline hemiacetal from a natural source. Inhibits Ca2+ currents and contractile responses in vitro.
Product Specific Literature References
Egenine: A possible intermediate in phthalideisoquinoline biogenesis: B. Gotzler, et al.; Tetrahedron 39, 577 (1983)
Synthesis of (-)-egenine (decumbensine) by asymmetric carbonyl addition: K.S. Rein & R.E. Gawley; Tetrahedron Lett. 31, 3711 (1990)
Synthesis of phthalide isoquinoline alkaloides (-)-egenine, (-)-corytensine, and (-)-bicuculline by asymmetric carbonyl addition of chiral dipole-stabilized organometallics: K.S. Rein & R.E. Gawley; J. Org. Chem. 56, 1564 (1991)
β-Phenylethylamines and the isoquinoline alkaloids: K.W. Bentley; Natural Prod. Rep. 11, 555 (1994), (Review)
1-Magnesiotetrahydroisoquinolyloxazolines as chiral nucleophiles in stereoselective additions to aldehydes: Auxiliary optimization, asymmetric synthesis of (+)-corlumine, (+)-bicuculline, (+)-egenine, and (+)-corytensine, and preliminary (13)C NMR studies: R.E. Gawley & P. Zhang; J. Org. Chem. 61, 8103 (1996) Abstract
Effects of alkaloids from Corydalis decumbens on contraction and electrophysiology of cardiac myocytes: S. Kadota, et al.; Phytother. Res. 10, 18 (1998)
Further Categories Containing This Product:
Calcium Signalling (Intracellular) Other Products • Alkaloids
 
 
ALX-350-105 Revised 05-Apr-08
Hellebrin
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SYNONYMS 3β-(O4-β-D-Glucopyranosyl-α-L-rhamnopyranosyloxy)-5,14-dihydroxy-19-oxo-5β,14-β-bufa-20,22-dienolide
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - ATPase Inhibitors
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ALX-350-105-M001   1 mg 45.00 USD Add To Cart
ALX-350-105-M005   5 mg 180.00 USD Add To Cart
Product Specification
FORMULA: C36H52O15
MW: 724.8
CAS NUMBER: 13289-18-4
SOURCE/HOST: Isolated from Helleborus sp.
PURITY: ≥99%
APPEARANCE: White solid.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: TOXIC.

Product Description
Cardiotonic glycoside which presents a bufadienolide-steroid structure. Besides ouabain (Prod. No. ALX-350-066), from the class of cardenolides hellebrin is the second unique water soluble cardiotonic glycoside. Extremely potent inhibitor of Na+/K+-ATPase  (sodium pump) blocking the active efflux of Na+ and reuptake of K+ of this membrane enzyme. Shows a positive inotropic effect by increasing the intracellular Ca2+ concentration. Strong immunosuppressor inhibiting T cell activity with much higher potency than cortisol or cyclosporin A (Prod. No. ALX-380-002). Induces caspase-dependent apoptosis.
Product Specific Literature References
Über Hellebrin, ein krystallisiertes Glykosid aus Radix Hellebori nigri: W. Karrer; Helv. Chim. Acta 26, 1353 (1943)
The structure of hellebrin: P. Muhr, et al.; Liebigs Ann. Org. Bioorg. Chem. 2, 443 (1995)
In search of ideal inotropic steroids: recent progress: K.R. Repke, et al.; Prog. Drug Res. 47, 9 (1996) Abstract
Exquisitely small amounts of nonglucocorticoid natural steroids suppress the human allogeneic T-cell response: P. Terness, et al.; Transplant Proc. 33, 547 (2001) Abstract
Apoptosis-mediated selective killing of malignant cells by cardiac steroids: maintenance of cytotoxicity and loss of cardiac activity of chemically modified derivatives: D. Daniel, et al.; Int. Immunopharmacol. 3, 1791 (2003) Abstract
Further Categories Containing This Product:
Na+/K+-ATPase/Related Products • Calcium Signalling (Intracellular) Other Products • Natural Products - Apoptosis Inducers & Inhibitors • Natural Products - Immunomodulators
 
 
ALX-804-089 Revised 27-Mar-08
Monoclonal Antibody to Calsequestrin (VIIID12)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calcium Signalling (Intracellular) Other Products
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ALX-804-089-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Dog
Others
CLONE: VIIID12
ISOTYPE: Mouse IgG2b
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Purified rabbit skeletal muscle sarcoplasmic reticulum.
SPECIFICITY: Recognizes mouse, rat, rabbit and dog type I (slow) and type II (fast) calsequestrin. Detects a band of ~63kDa by Western blot.
APPLICATION: Immunocytochemistry
Western Blot  (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-092 Revised 22-May-08
Monoclonal Antibody to Na+/Ca2+ Exchanger (C2C12)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calcium Signalling (Intracellular) Other Products
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ALX-804-092-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
Dog
Others
CLONE: C2C12
ISOTYPE: Mouse IgM
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Purified dog cardiac Na+/ Ca2+exchanger.
SPECIFICITY: Recognizes human, rat, dog, rabbit and guinea pig Na+/ Ca2+ exchanger. The epitope for this antibody is on the intracellular side of the plasma membrane (aa 371-525). Detects a band of ~120kDa (mature exchanger), as well as bands of ~70kDa (proteolytic fragment) and ~160kDa (non-reduced exchanger) by Western blot.
APPLICATION: Immunocytochemistry (1:200)
Immunohistochemistry
Immunoprecipitation
Western Blot (1:1’000; not recommended for rat tissue)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Description
The Na+/Ca2+ exchanger of cardiac sarcolemma rapidly transports Ca2+ during excitation-concentration coupling and is the dominant myocardial Ca2+ efflux mechanism.
Product Specific Literature References
Distribution of the Na+/Ca2+ exchange protein in mammalian cardiac myocytes: An immunofluorescence and immunocolloidal gold-labelling study: J.S. Frank, et al.; J. Cell. Biol. 117, 337 (1992) Abstract
Mapping of the cardiac sodium-calcium exchanger with monoclonal antibodies: H. Porzig, et al.; Am. J. Physiol. 265, C748 (1993) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-093 Revised 21-May-08
Monoclonal Antibody to Phospholamban (2D12)
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SYNONYMS anti-PLB (2D12) MAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calcium Signalling (Intracellular) Other Products
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ALX-804-093-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
Dog
Pig
Others
CLONE: 2D12
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 2-25 (D2KVQYLTRSAIRRASTIEMPQQAR25) of dog PLB (phospholamban).
SPECIFICITY: Recognizes human, mouse, rat, sheep, dog, pig and guinea pig PLB. Recognizes an epitope between aa 9-17 of dog PLB. Detects bands of ~25kDa (non-reducing conditions; representing the pentameric form of PLB) and ~5kDa (reducing conditions; representing the monomeric form of PLB) by Western blot.
APPLICATION: Immunocytochemistry (20µg/ml)
Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot (2µg/ml)
Functional Application (increases the calcium uptake in ventricular SR vesicles).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Description
The antibody can also be used to increase calcium uptake in ventricular SR vesicle analogous to the phosphorylation of phospholamban following adrenergic stimulation.
Product Specific Literature References
Phospholamban mediates the b-adrenergic-enhanced Ca2+ uptake in mammalian ventricular myocytes: J.S.K. Sham, et al.; Am. J. Physiol. 261, H1344 (1991) Abstract
Phospholamban expressed in slow-twitch and chronically stimulated fast-twitch muscles minimally affects calcium affinity of sarcoplasmic reticulum Ca(2+)-ATPase: F.N. Briggs, et al.; J. Biol. Chem. 267, 26056 (1992) Abstract; Full Text
Residues 2-25 of phospholamban are insufficient to inhibit Ca2+ transport ATPase of cardiac sarcoplasmic reticulum: L.R. Jones and L.J. Field; J. Biol. Chem. 268, 11486 (1993) Abstract; Full Text
Comparative studies of cardiac and skeletal sarcoplasmic reticulum ATPases. Effect of a phospholamban antibody on enzyme activation by Ca2+: T. Cantilina, et al.; J. Biol. Chem. 268, 17018 (1993) Abstract; Full Text
The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle: A.O. Jorgensen, et al.; J. Cell Biol. 120, 969 (1993) Abstract; Full Text
Molecular and physiological alterations in murine ventricular dysfunction: H.A. Rockman, et al.; PNAS 91, 2694 (1994) Abstract; Full Text
Mechanisms of altered excitation-contraction coupling in canine tachycardia-induced heart failure, I: experimental studies: B. O’Rourke, et al.; Circ. Res. 84, 562 (1999) Abstract; Full Text
Overexpression of the sarcoplasmic reticulum Ca(2+)-ATPase improves myocardial contractility in diabetic cardiomyopathy: S.U. Trost, et al.; Diabetes 51, 1166 (2002) Abstract; Full Text
Ca(2+) signaling in cardiac myocytes overexpressing the alpha(1) subunit of L-type Ca(2+) channel: L.S. Song, et al.; Circ. Res. 90, 174 (2002) Abstract; Full Text
Abnormal Ca2+ release, but normal ryanodine receptors, in canine and human heart failure: M.T. Jiang, et al.; Circ. Res. 91, 1015 (2002) Abstract; Full Text
Targeting phospholamban by gene transfer in human heart failure: F. del Monte, et al.; Circulation 105, 904 (2002) Abstract; Full Text
Type 1 phosphatase, a negative regulator of cardiac function: A.N. Carr, et al.; Mol. Cell. Biol. 22, 4124 (2002) Abstract; Full Text
Altered dose response to beta-agonists in SERCA1a-expressing hearts ex vivo and in vivo: S. Huke, et al.; Am. J. Physiol. Heart Circ. Physiol. 283, H958 (2002) Abstract; Full Text
Defective intracellular Ca(2+) signaling contributes to cardiomyopathy in Type 1 diabetic rats: K.M. Choi, et al.; Am. J. Physiol. Heart Circ. Physiol. 283, H1398 (2002) Abstract; Full Text
Interaction between increased SERCA2a activity and beta -adrenoceptor stimulation in adult rabbit myocytes: B. Chaudhri, et al.; Am. J. Physiol. Heart Circ. Physiol. 283, H2450 (2002) Abstract; Full Text
Targeted inhibition of Ca2+/calmodulin-dependent protein kinase II in cardiac longitudinal sarcoplasmic reticulum results in decreased phospholamban phosphorylation at threonine 17: Y. Ji, et al.; J. Biol. Chem. 278, 25063 (2003) Abstract; Full Text
Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation: K.Y. Xu, et al.; J. Biol. Chem. 278, 41798 (2003) Abstract; Full Text
Mechanism of enhanced cardiac function in mice with hypertrophy induced by overexpressed Akt: Y.K. Kim, et al.; J. Biol. Chem. 278, 47622 (2003) Abstract; Full Text
Regional alterations in protein expression in the dyssynchronous failing heart: D.D. Spragg, et al.; Circulation 108, 929 (2003) Abstract; Full Text
Sarcoplasmic reticulum calcium defect in Ras-induced hypertrophic cardiomyopathy heart: M. Zheng, et al.; Am. J. Physiol. Heart Circ. Physiol. 286, H424 (2004) Abstract; Full Text
Autologous stem cell transplantation for myocardial repair: J. Liu, et al.; Am. J. Physiol. Heart Circ. Physiol. 287, H501 (2004) Abstract; Full Text
Doxycycline inducible expression of SERCA2a improves calcium handling and reverts cardiac dysfunction in pressure overload-induced cardiac hypertrophy: J. Suarez, et al.; Am. J. Physiol. Heart Circ. Physiol. 287, H2164 (2004) Abstract; Full Text
The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm: T.L. Radzyukevich, et al.; Am. J. Physiol. Cell Physiol. 287, C1300 (2004) Abstract; Full Text
Frequency- and afterload-dependent cardiac modulation in vivo by troponin I with constitutively active protein kinase A phosphorylation sites: E. Takimoto, et al.; Circ. Res. 94, 496 (2004) Abstract; Full Text
Alterations in myofilament function contribute to left ventricular dysfunction in pigs early after myocardial infarction: J. van der Velden, et al.; Circ. Res. 95, e85 (2004) Abstract; Full Text
Adenylyl cyclase type VI gene transfer reduces phospholamban expression in cardiac myocytes via activating transcription factor 3: M.H. Gao, et al.; J. Biol. Chem. 279, 38797 (2004) Abstract; Full Text
Chronic ventricular myocyte-specific overexpression of angiotensin II type 2 receptor results in intrinsic myocyte contractile dysfunction: M. Nakayama, et al.; Am. J. Physiol. Heart Circ. Physiol. 288, H317 (2005) Abstract; Full Text
Genetic manipulation of calcium-handling proteins in cardiac myocytes. I. Experimental studies: P. Coutu and J.M. Metzger; Am. J. Physiol. Heart Circ. Physiol. 288, H601 (2005) Abstract; Full Text
Differential activation of stress-response signaling in load-induced cardiac hypertrophy and failure: B.A. Rothermel, et al.; Physiol. Genomics 23, 18 (2005) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-201-383 Revised 18-Mar-08
Nucleobindin-2 (mouse) (recombinant) (His)
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SYNONYMS NUCB2 (mouse) (recombinant) (His)
DNA-binding Protein NEFA (mouse) (recombinant) (His)
Nesfatin (mouse) (recombinant) (His)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calcium Signalling (Intracellular) Other Products
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ALX-201-383-C010   10 µg 350.00 USD Add To Cart
ALX-201-383-C050   50 µg 490.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. Mature human nucleobindin-2 (aa 25-420) is fused at the C-terminus to a His-tag.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS, pH 7.2.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P81117: Nucleobindin-2 (mouse) [Precursor]
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins • Food Intake Modulators Other Products
 
 
ALX-210-290 Revised 11-May-07
Polyclonal Antibody to Calsequestrin
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calcium Signalling (Intracellular) Other Products
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ALX-210-290-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
Dog
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Purified dog cardiac calsequestrin.
SPECIFICITY: Recognizes human, mouse, rat, dog and sheep calsequestrin. Recognizes both cardiac and skeletal muscle calsequestrin. Detects a band of ~55kDa by Western blot.
CROSSREACTIVITY PROTEINS: Additional bands at ~97kDa may be observed and have been reported to be calsequestrin-like proteins.
APPLICATION: Immunoprecipitation
Western Blot (1:2'500)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Developmental changes in cardiac sarcoplasmic reticulum in sheep: L. Mahony & L.R. Jones; J. Biol. Chem. 261, 15257 (1986) Abstract; Full Text
Complex formation between junctin, triadin, calsequestrin, and the ryanodine receptor. Proteins of the cardiac junctional sarcoplasmic reticulum membrane: L. Zhang, et al.; J. Biol. Chem. 272, 23389 (1997) Abstract; Full Text
Identification of triadin 1 as the predominant triadin isoform expressed in mammalian myocardium: Y.M. Kobayashi & L.R. Jones; J. Biol. Chem. 274, 28660 (1999) Abstract; Full Text
General Information
The sarcoplasmic reticulum (SR) is in part responsible for maintaining the level of intracellular Ca2+ in cardiac and skeletal muscle by storing and releasing Ca2+. Several intralumenal SR Ca2+ binding proteins have been identified, the most prominent of these is calsequestrin. Calsequestrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize Ca2+ near the junctional face of the terminal cisternae from which Ca2+ can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for Ca2+. Two mammalian isoforms of calequestrin exist, a cardiac and a skeletal muscle isoform, which are the products of different genes and are 63% identical. This antibody recognizes both cardiac and skeletal muscle calsequestrin from a variety of species.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-292 Revised 13-Jan-05
Polyclonal Antibody to Na+/Ca2+/K+Exchanger (rat)
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SYNONYMS anti-NCKX2 (rat) PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calcium Signalling (Intracellular) Other Products
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ALX-210-292-R200   200 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 90-102 (D90LNDKIRDYTPQP102C) of rat NCKX2 (Na+ / Ca2+/K+ exchanger).
SPECIFICITY: Recognizes rat NCKX2. Detects a band of ~78kDa by Western blot.
APPLICATION: Immunohistochemistry (frozen sections (1:100))
Western Blot (1:100)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-163-008.
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Product Description
Na+/Ca2+-K+ exchanger proteins (NCKX1) have been reported to occur in retinal photoreceptors and in brain (NCKX2). Unlike the abundant and widely expressed Na+/Ca2+ exchanger which catalyzes the exchange of 3 Na+ ions for 1 Ca2+ ion to modulate intracellular Ca2+ levels, NCKX isoforms exchange 4 Na+ ions for 1 Ca2+ and 1 K+ ion. The Na+/Ca2+-K+ exchanger uses the transmembrane Na+ gradient to catalyze countertransport of Ca2+ in a K+-dependent mechanism against its electrochemical gradient. It is hypothesized that NCKX2 plays a significant role in calcium homeostasis and the regulation of neuronal function.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-460 Revised 26-Apr-07
Polyclonal Antibody to Nucleobindin-2 (mouse)
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SYNONYMS anti-NUCB2 (mouse) PAb
anti-DNA-binding Protein NEFA (mouse) PAb
anti-Nesfatin (mouse) PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calcium Signalling (Intracellular) Other Products
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ALX-210-460-C100   100 µg 310.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Purified IgG.
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant mouse nucleobindin-2 (NUCB2).
SPECIFICITY: Recognizes mouse nucleobindin-2.
APPLICATION: ELISA (direct and indirect: 1:2’000-1:5’000)
Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 1-2 weeks when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Food Intake Modulators Other Products • Polyclonal Antibodies
 
 
ALX-201-259 Revised 26-Feb-07