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Stress & Heat Shock Proteins
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ALX-804-810 Revised 24-Mar-05
Monoclonal Antibody to HSP70 (BB70)
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SYNONYMS anti-Heat Shock Protein 70 MAb (BB70)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY HSP70 / DnaK / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-810-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
Dog
Pig
Monkey
Others
CLONE: BB70
ISOTYPE: Mouse IgG2a
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: >95% (SDS-PAGE)
FORMULATION: Liquid. Purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: Chicken HSP70 (heat shock protein 70)/HSP90 (heat shock protein 90) complex.
SPECIFICITY: Recognizes both free and complexed human, mouse, rat, sheep, dog, pig, monkey, fish, guinea pig, hamster, rabbit, chicken, xenopus, drosophila and yeast HSP70 and HSC70. Detects bands of ~72 and ~73 kDa by Western blot, corresponding to HSP70 and HSC70, respectively.
APPLICATION: Immunohistochemistry (works on endogenous)
Immunoprecipitation (works on endogenous)
Western Blot (works on endogenous)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least one year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Identification of a 60-kilodalton stress-related protein, p60, which interacts with hsp90 and hsp70: D.F. Smith, et al.; Mol. Cell. Biol. 13, 869 (1993) Abstract; Full Text
Mutational analysis of the hsp70-interacting protein Hip: V. Prapapanich, et al.; Mol. Cell. Biol. 16, 6200 (1996) Abstract; Full Text
Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly: V. Prapapanich, et al.; Mol. Cell. Biol. 18, 944 (1998) Abstract; Full Text
Hop modulates Hsp70/Hsp90 interactions in protein folding: B.D. Johnson, et al.; J. Biol. Chem. 273, 3679 (1998) Abstract; Full Text
A role for heat shock protein 27 in CTL-mediated cell death: P.J. Beresford, et al.; J. Immunol. 161, 161 (1998) Abstract; Full Text
Hop as an adaptor in the heat shock protein 70 (Hsp70) and hsp90 chaperone machinery: S. Chen & D.F. Smith; J. Biol. Chem. 273, 35194 (1998) Abstract; Full Text
Repression of heat shock transcription factor HSF1 activation by HSP90 (HSP90 complex) that forms a stress-sensitive complex with HSF1: J. Zou, et al.; Cell 94, 471 (1998) Abstract; Full Text
Identification of genes that modify ataxin-1-induced neurodegeneration: P. Fernandez-Funez, et al.; Nature 408, 101 (2000) Abstract; Full Text
Induction of a heat shock factor 1-dependent stress response alters the cytotoxic activity of hsp90-binding agents: R. Bagatell, et al.; Clin. Cancer Res. 6, 3312 (2000) Abstract; Full Text
Hsp90 is required for c-Mos activation and biphasic MAP kinase activation in Xenopus oocytes: D.L. Fisher, et al.; EMBO J. 19, 1516 (2000) Abstract
Mutations in Drosophila heat shock cognate 4 are enhancers of Polycomb: R. Mollaaghababa, et al.; PNAS 98, 3958 (2001) Abstract; Full Text
Modulation of Drosophila heat shock transcription factor activity by the molecular chaperone DROJ1: G. Marchler & C. Wu; EMBO J. 20, 499 (2001) Abstract
Co-translational folding of caspase-activated DNase with Hsp70, Hsp40, and inhibitor of caspase-activated DNase: H. Sakahira & S. Nagata; J. Biol. Chem. 277, 3364 (2002) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
The ubiquitin-related BAG-1 provides a link between the molecular chaperones Hsc70/Hsp70 and the proteasome: J. Luders, et al.; J. Biol. Chem. 275, 4613 (2000) Abstract; Full Text
Further Categories Containing This Product:
Endoplasmatic Reticulum Stress • Monoclonal Antibodies
 
 
ALX-804-068 Revised 31-Aug-07
Monoclonal Antibody to HSP27 (G3.1)
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SYNONYMS anti-Heat Shock Protein 27 MAb (G3.1)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Small Heat Shock Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-068-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
Dog
CLONE: G3.1
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Partially purified HSP27 (heat shock protein 27).
SPECIFICITY: Recognizes human, mouse (weak), rat and dog HSP27. Detects a band of ~24kDa by Western blot.
APPLICATION: ELISA
Immunohistochemistry (paraffin sections (1:500))
Immunoprecipitation
Western Blot (1:500)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Detection of a Mr 24,000 estrogen-related protein in human breast cancer by monoclonal antibodies: D.J. Adams, et al.; Cancer Res. 43, 4297 (1983) Abstract
General Information
HSP27 also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that HSP27 may be a useful marker in classifying various hormone sensitive tumors.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-588 Revised 20-Nov-07
Monoclonal Antibody to HSP27 (phosphorylated) (pSer82) (5B9)
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SYNONYMS anti-Heat Shock Protein 27 (phosphorylated) (pSer82) MAb (5B9)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Small Heat Shock Proteins / Related Products
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ALX-804-588-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 5B9
ISOTYPE: Mouse IgG2a
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to aa 79-85 (R79QLpSSGV85) of human HSP27 (heat shock protein 27) phosphorylated at Ser82.
SPECIFICITY: Recognizes human, mouse, rat and dog phosphorylated HSP27.
APPLICATION: Immunohistochemistry
Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL: Yes (Prod. No. ALX-840-031).
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-584 Revised 31-Oct-06
Monoclonal Antibody to HSP65 (mycobacterial) (4H11)
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SYNONYMS anti-Heat Shock Protein 65 (mycobacterial) MAb (4H11)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY HSP60 / GroEL / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-584-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Others
CLONE: 4H11
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS, pH 7.2, containing 0.1mM PMSF and 50% glycerol.
IMMUNOGEN: Recombinant Mycobacterium bovis BCG HSP65 (heat shock protein 65).
SPECIFICITY: Recognizes M. bovis and M. leprae HSP65. Detects a band of ~65kDa by Western blot. Does not cross-react with the mammalian HSP60 homologs from human, mouse, rat and monkey.
APPLICATION: Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 2-4 weeks when stored at +4°C.
HANDLING: For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-075 Revised 24-Sep-07
Monoclonal Antibody to HSP70 (2A4)
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SYNONYMS anti-Heat Shock Protein 70 MAb (2A4)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY HSP70 / DnaK / Related Products
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ALX-804-075-R050   50 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Others
CLONE: 2A4
ISOTYPE: Mouse IgM
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Human HSP70 (heat shock protein 70).
SPECIFICITY: Recognizes HSP70, HSC70, and following heat shock, HSP72 from a broad range of species including human, mouse, avian, frog, fish, drosophila, yeast and wheat HSP70. Detects bands from ~70 to ~72kDa by Western blot, representing different members of the HSP70 family.
CROSSREACTIVITY PROTEINS: HSP70, HSC70, HSP72 (following heat shock)
APPLICATION: Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections)
Western Blot (1:5’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION 2D-gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-076 Revised 01-Oct-07
Monoclonal Antibody to HSP70 (human) (4G4)
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SYNONYMS anti-Heat Shock Protein 70 (human) MAb (4G4)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY HSP70 / DnaK / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-076-R050   50 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 4G4
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites diluted in PBS.
IMMUNOGEN: Recombinant human HSP70 (heat shock protein 70).
SPECIFICITY: Recognizes human HSP70.
APPLICATION: Immunoprecipitation
Western Blot (1:5'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-586 Revised 31-Oct-06
Monoclonal Antibody to HSP71 (mycobacterial) (5A8)
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SYNONYMS anti-Heat Shock Protein 71 (mycobacterial) MAb (5A8)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY HSP70 / DnaK / Related Products
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ALX-804-586-C200   200 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Others
CLONE: 5A8
ISOTYPE: Mouse IgG1
PURITY DETAIL: Protein G-affinity purified antibody.
FORMULATION: Liquid. Protein G purfied antibody in PBS, pH 7.2, containing 0.1mM PMSF and 50% glycerol.
IMMUNOGEN: Recombinant Mycobacterium tuberculosis HSP71 (heat shock protein 71).
SPECIFICITY: Recognizes M. bovis and M. leprae HSP71. Detects a band of ~71kDa by Western blot. Does not cross-react with eukaryotic HSC70, HSP70 or DnaK from E. coli.
APPLICATION: Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stability at +4°C: 2-4 weeks.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.
POSITIVE CONTROL: M. bovis or M. leprae samples can be used as positive control.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-078 Revised 29-Jul-08
Monoclonal Antibody to HSP90 (3B6)
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SYNONYMS anti-Heat Shock Protein 90 MAb (3B6)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY HSP90 / HtpG / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-078-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 3B6
ISOTYPE: Mouse IgG2b
FORMULATION: Liquid. Ascites fluid diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Purified mouse HSP90 (heat shock protein 90).
SPECIFICITY: Recognizes human, mouse, rat and bovine HSP90. Detects a band of ~90kDa by Western blot. Does not cross-react with chicken HSP90.
APPLICATION: Western Blot (1:500)
For immunoprecipitation use MAb to HSP90 (3G3) (Prod. No. ALX-804-079).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Chaperone-mediated Autophagy [CMA] • Monoclonal Antibodies
 
 
ALX-804-079 Revised 14-Sep-07
Monoclonal Antibody to HSP90 (3G3)
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SYNONYMS anti-Heat Shock Protein 90 MAb (3G3)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY HSP90 / HtpG / Related Products
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ALX-804-079-R400   400 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE: 3G3
ISOTYPE: Mouse IgM
PURITY DETAIL: Ammonium sulfate/ascites suspension.
FORMULATION: Liquid. Contains no preservatives.
IMMUNOGEN: Purified mouse HSP90 (heat shock protein 90).
SPECIFICITY: Recognizes human, mouse, rat, rainbow trout and chicken HSP90.
APPLICATION: Immunoprecipitation
For Western blot use MAb to HSP90 (3B6) (Prod. No. ALX-804-078).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Before use, centrifuge suspension (4’000 x g for 15 min.) and discard supernatant. Resuspend pellet in 200µl PBS, pH 7.2. If desired, add 0.05% sodium azide. Stable for 6 months after PBS resuspension.
Removal of residual ammonium sulfate is not usually necessary for IP procedure; for other uses it may be removed by dialysis or equivalent method.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Evidence that the 90-kDa heat shock protein (HSP90) exists in cytosol in heteromeric complexes containing HSP70 and three other proteins with Mr of 63,000, 56,000, and 50,000: G.H. Perdew; J. Biol. Chem. 266, 6708 (1991) Abstract; Full Text
Further Categories Containing This Product:
Chaperone-mediated Autophagy [CMA] • Monoclonal Antibodies
 
 
ALX-804-023 Revised 31-Aug-07
Monoclonal Antibody to HSP90 Co-chaperone (JJ3)
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SYNONYMS anti-Telomerase-binding Protein p23 MAb (JJ3)
anti-Progesterone Receptor Complex p23 MAb (JJ3)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY HSP90 / HtpG / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-023-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Others
CLONE: JJ3
ISOTYPE: Mouse IgG2a
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human HSP90 Co-chaperone.
SPECIFICITY: Recognizes human, mouse, chicken, rabbit and guinea pig HSP90 Co-chaperone. Detects a band of ~23kDa by Western blot.
APPLICATION: Immunocytochemistry
Immunoprecipitation (use on free or complexed HSP90 Co-chaperone)
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Folding of the glucocorticoid receptor by the reconstituted Hsp90-based chaperone machinery. The initial hsp90.p60.hsp70-dependent step is sufficient for creating the steroid binding conformation.: K.D. Dittmar & W.B. Pratt; J. Biol. Chem. 272, 13047 (1997) Abstract
Hepadnavirus assembly and reverse transcription require a multi-component chaperone complex which is incorporated into nucleocapsids  : J.Hu, D. O. Toft & C. Seeger; EMBO Journal 16, 59 (1997) Full Text
General Information
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex.
Cloned human p23 encodes a protein of 160 aa that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.
Further Categories Containing This Product:
Chaperone-mediated Autophagy [CMA] • Monoclonal Antibodies
 
 
ALX-804-808 Revised 13-Aug-07
Monoclonal Antibody to HSP90α/β (H90-10)
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