ALEXIS Biochemicals: SuperFasLigand™, Soluble (human) (recombinant)

ALX-522-020

Revised 16-May-06

SuperFasLigand™, Soluble (human) (recombinant)
SYNONYMS	APO-1L, Soluble (human) (recombinant) CD95L, Soluble (human) (recombinant) CD178, Soluble (human) (recombinant) TNFSF 6, Soluble (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	FasL

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-020-C005		5 µg	250.00 USD
ALX-522-020-3005	SuperPack	3x5 µg	500.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
MW:	~32kDa (nonglycosylated), ~35kDa (glycosylated).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG^®-tag. Glycosylation of rhsSuperFasLigand^TMis similar to natural human FasL.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with cell culture medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to human, mouse and rat Fas (CD95; APO-1).
BIOLOGICAL ACTIVITY:	Kills Fas-sensitive cells at concentrations of >1ng/ml. Note: Does not require enhancer.
SPECIFIC ACTIVITY:	ED₅₀: 1ng/ml (A20 cells).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Images

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Product Specific Literature References

Fas ligand (CD95L) protects neurons against perforin-mediated T lymphocyte cytotoxicity: I. Medana, et al.; J. Immunol. 167, 674 (2001) Abstract; Full Text
Tumor-cell resistance to death receptor-induced apoptosis through mutational inactivation of the proapoptotic Bcl-2 homolog Bax: H. LeBlanc, et al.; Nat. Med. 8, 274 (2002) Abstract
Inhibition of metalloproteinase cleavage enhances the cytotoxicity of Fas ligand: P.G. Knox, et al.; J. Immunol. 170, 677 (2003) Abstract; Full Text
NF-kappa B is required for surface Ig-induced Fas resistance in B cells: B.R. Schram & T.L. Rothstein; J. Immunol. 170, 3118 (2003) Abstract; Full Text
TNF-related apoptosis-inducing ligand (TRAIL) frequently induces apoptosis in Philadelphia chromosome-positive leukemia cells: K. Uno, et al.; Blood 101, 3658 (2003) Abstract
Fas Ligand Down-Regulates Cytokine-Induced Fas Receptor Expression on Insulinoma (NIT-1), But Not Islet Cells, from Autoimmune Nonobese Diabetic Mice: P. Augstein, et al.; Endocrinology 145, 2747 (2004) Abstract
In vitro engagement of CD3 and CD28 corrects T cell defects in chronic lymphocytic leukemia: M. Bonyhadi, et al.; J. Immunol. 174, 2366 (2005) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Note: Results using rhsSuperFasLigand™ may differ from those obtained with agonistic antibodies!

Swiss-Prot link P48023: FasL (human)
AfCs Signalling Gateway link A002830: FasL (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Mature T cells of autoimmune lpr/lpr mice have a defect in antigen-stimulated suicide: J.H. Russel, et al.; PNAS 90, 4409 (1993) Abstract
A role for CD95 ligand in preventing graft rejection: D. Bellgrau, et al.; Nature 377, 630 (1995) Abstract
Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape: M. Hahne, et al.; Science 274, 1363 (1996) Abstract
Apoptosis by death factor: S. Nagata; Cell 88, 355 (1997) Abstract
Death receptors: signaling and modulation: A. Ashkenazi & V.M. Dixit; Science 281, 1305 (1998) Abstract

Further Categories Containing This Product:

CD Proteins • Recombinant Proteins / Fusion Proteins

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