ALEXIS Biochemicals: SuperKillerTRAIL™, Soluble (human) (recombinant)

ALX-201-115

Revised 06-Oct-08

SuperKillerTRAIL™, Soluble (human) (recombinant)
SYNONYMS	Apo-2L, Soluble (human) (recombinant) (CC-mutant) TNFSF 10, Soluble (human) (recombinant) (CC-mutant) CD253, Soluble (human) (recombinant) (CC-mutant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	TRAIL [Apo-2L]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-115-C010		10 µg	360.00 USD
ALX-201-115-3010	SuperPack	3x10 µg	720.00 USD

Product Specification

SOURCE/HOST:	Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.
CONCENTRATION:	0.5mg/ml.
PURITY:	≥98% (SDS-PAGE)
PURITY DETAIL:	Affinity purified. Sterile filtered.
FORMULATION:	Liquid. In 20mM HEPES, pH 7.9, containing 120mM NaCl, 0.01% Tween 20, 1% sucrose, 1mM DTT.
ENDOTOXIN CONTENT:	<0.02EU/µg purified protein (LAL test; Bio Whittaker).
SPECIFICITY:	Binds to human TRAIL receptors.
BIOLOGICAL ACTIVITY:	Induces apoptosis at concentrations of >1ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. Shows superior activity on human cell lines that require extensive cross-linking of TRAIL-Rs for killing (e.g. Jurkat).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C. For a special storage and dilution buffer see Prod. No. ALX-505-005. Working dilution: 2-100ng/ml.
HANDLING:	Avoid freeze/thaw cycles.

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Product Specific Literature References

Synthetic lethal targeting of MYC by activation of the DR5 death receptor pathway: Y. Wang, et al.; Cancer Cell 5, 501 (2004) Abstract
Sulforaphane targets pancreatic tumor-initiating cells by NF-{kappa}B-induced anti-apoptotic signaling: G. Kallifatidis, et al.; Gut Epub ahead of print, (2008) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P50591: TRAIL (human)
AfCS Signalling Gateway link A002862: TRAIL (mouse)