ALEXIS Biochemicals: TL1A, Soluble (mouse) (recombinant)

ALX-522-105

Revised 27-Mar-08

TL1A, Soluble (mouse) (recombinant)
SYNONYMS	TNFSF 15, Soluble (mouse) (recombinant) VEGI, Soluble (mouse) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	HVEM & LIGHT / LT-betaR / DcR3 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-105-C010		10 µg	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse
MW:	~25kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of mouse TL1A (aa 94-252) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds mouse DR3.
APPLICATION:	ELISA Flow Cytometry (use together with Enhancer for Ligands (Prod. No. ALX-804-034) to detect human and mouse DR3 and DcR3).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

General Information

TL1A (TNFSF15), a ligand belonging to the tumor necrosis factor (TNF) family, is expressed predominantly by endothelial cells and monocytes [1]. TL1a is upregulated by the proinflammatory cytokines TNF and IL-1 and also by immune complexes (IC) [2]. TL1 functions in T cell costimulation and Th1 polarization. On activated T cells, TL1A functions specifically via its surface-bound receptor DR3, (a member of the death-domain containing TNF receptor family) to promote cell survival and secretion of proinflammatory cytokines (1). The secreted decoy receptor 3 (DcR3), a soluble protein of the tumor necrosis factor receptor (TNFR) superfamily blocks the action of TL1A [3]. TL1A can be released to circulate as a homotrimeric soluble form [4]. Activation of DR3 by TL1A induced the formation of a signaling complex containing TRADD, TRAF2, and RIP and activated the NF-κB and the ERK, JNK, and p38 mitogen-activated protein kinase pathways [5]. The TL1A/DR3 pathway plays an important role in Th1-mediated intestinal diseases, such as Crohn’s disease [6].
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q8K3Y8: TL1A (mouse) FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

[1] TL1A is a TNF-like ligand for DR3 and TR6/DcR3 and functions as a T cell costimulator: T.S. Migone, et al.; Immunity 16, 479 (2002) Abstract
[2] Soluble TNF-like cytokine (TL1A) production by immune complexes stimulated monocytes in rheumatoid arthritis: M.A. Cassatella, et al.; J. Immunol. 178, 7325 (2007) Abstract
[3] Enhanced adhesion of monocytes via reverse signaling triggered by decoy receptor 3: M.J. Hsu, et al.; Exp. Cell Res. 292, 241 (2004) Abstract
[4] Identification of naturally secreted soluble form of TL1A, a TNF-like cytokine: S. Kim & L. Zhang; J. Immunol. Methods 298, 1 (2005) Abstract
[5] TL1A-induced NF-kappaB activation and c-IAP2 production prevent DR3-mediated apoptosis in TF-1 cells: L. Wen, et al.; J. Biol. Chem. 278, 39251 (2003) Abstract
[6] Expression, localization, and functional activity of TL1A, a novel Th1-polarizing cytokine in inflammatory bowel disease: G. Bamias, et al.; J. Immunol. 171, 4868 (2003) Abstract