ALEXIS Biochemicals: TNF-α, Soluble (human) (recombinant)

ALX-522-008

Revised 18-Sep-07

TNF-α, Soluble (human) (recombinant)
SYNONYMS	Tumor Necrosis Factor-α, Soluble (human) (recombinant) TNFSF 2, Soluble (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	TNF-alpha

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-008-C050		50 µg	290.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
MW:	~19kDa (SDS-PAGE).
SOURCE/HOST:	Produced in E. coli. The extracellular domain of human TNF-α (tumor necrosis factor-α) (aa 85-223) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG^®-tag.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to human and mouse TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-060), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.
BIOLOGICAL ACTIVITY:	Exerts its biological activity in a concentration range of 0.1-1ng/ml (WEHI 164 cells).
SPECIFIC ACTIVITY:	ED₅₀: 0.1ng/ml (WEHI 164 cells)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Specific Literature References

Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998) Abstract; Full Text
A second step of chemotaxis after transendothelial migration: keratinocytes undergoing apoptosis release IFN-gamma-inducible protein 10, monokine induced by IFN-gamma, and IFN-gamma-inducible alpha-chemoattractant for T cell chemotaxis toward epidermis in: S. Klunker, et al.; J. Immunol. 171, 1078 (2003) Abstract; Full Text
Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes: O. Micheau & J. Tschopp; Cell 114, 181 (2003) Abstract
Insulin-secreting beta-cell dysfunction induced by human lipoproteins: M.E. Roehrich, et al.; J. Biol. Chem. 278, 18368 (2003) Abstract; Full Text
BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004) Abstract
The chemokine receptor CCR7 activates in dendritic cells two signaling modules that independently regulate chemotaxis and migratory speed: L. Riol-Blanco, et al.; J. Immunol. 174, 4070 (2005) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P01375: TNF-α (human) (precursor)
AfCS Signalling Gateway link A002291: TNF-α (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

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