ALEXIS Biochemicals: CD40 (human):COMP (human) (recombinant)

MegaAPRIL™, Soluble (human) (recombinant)
SYNONYMS	APRIL, Soluble (human) (recombinant):ACRP30headless (human) A-Proliferation-inducing Ligand (human) (recombinant):ACRP30headless (human) TNFSF 13, Soluble (human) (recombinant):ACRP30headless (human) CD256, Soluble (human) (recombinant):ACRP30headless (human)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	APRIL

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-035-3010		3x10 µg	460.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
MW:	~34kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human APRIL (aa 88-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a FLAG^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to human and mouse BCMA and TACI. Binds to proteoglycans.
BIOLOGICAL ACTIVITY:	Stimulates proliferation of serum-starved NIH 3T3 cells. Stimulates B cell proliferation.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Images

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Product Specific Literature References

DCs induce CD40-independent immunoglobulin class switching through BLyS and APRIL: M.B. Litinskiy, et al.; Nat. Immunol. 3, 822 (2002) Abstract
APRIL modulates B and T cell immunity: J.V. Stein, et al.; J. Clin. Invest. 109, 1587 (2002) Abstract; Full Text
Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol. 172, 3268 (2004) Abstract; Full Text
Potential role of APRIL as autocrine growth factor for megakaryocytopoiesis: D. Bonci, et al.; Blood 104, 3169 (2004) Abstract; Full Text
BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway: A. Craxton, et al.; J. Exp. Med. 202, 1363 (2005) Abstract
APRIL and BAFF promote increased viability of replicating human B2 cells via mechanism involving cyclooxygenase 2: P.K. Mongini, et al.; J. Immunol. 176, 6736 (2006) Abstract; Full Text
TACI regulates IgA production by APRIL in collaboration with HSPG: D. Sakurai, et al.; Blood 109 , 2961 (2007) Abstract
BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway: T. Endo, et al.; Blood 109, 703 (2007) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link O75888: APRIL (human)
AfCS Signalling Gateway link A000305: APRIL (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth: M. Hahne, et al.; J. Exp. Med. 188, 1185 (1998) Abstract
Interaction of the TNF homologues BLyS and APRIL with the TNF receptor homologues BCMA and TACI: S.A. Marsters, et al.; Curr. Biol. 10, 785 (2000) Abstract
A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med. 192, 1677 (2000) Abstract
Tumor necrosis factor (TNF) receptor superfamily member TACI is a high affinity receptor for TNF family members APRIL and BLyS: Y. Wu, et al.; J. Biol. Chem. 275, 35478 (2000) Abstract
APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity: G. Yu, et al.; Nat. Immunol. 1, 252 (2000) Abstract
B-cell maturation protein, which binds the tumor necrosis factor family members BAFF and APRIL, is dispensable for humoral immune responses: S. Xu & K.P. Lam; Mol. Cell. Biol. 21, 4067 (2001) Abstract

Further Categories Containing This Product:

Recombinant Proteins/Fusion Proteins

ALX-270-443

Revised 05-May-08

AS601245
SYNONYMS	1,3-Benzothiazol-2-yl-(2-{[2-(3-pyridinyl)ethyl]amino}-4-pyrimidinyl) acetonitrile JNK Inhibitor V
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	JNK [SAPK1]/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-443-M001		1 mg	50.00 USD
ALX-270-443-M005		5 mg	150.00 USD
ALX-270-443-M025		25 mg	520.00 USD

Product Specification

FORMULA:	C₂₀H₁₆N₆S
MW:	372.5
CAS NUMBER:	345987-15-7
PURITY:	≥95% (HPLC)
APPEARANCE:	White to yellow solid.
PURITY DETAIL:	Sum of two isomers.
SOLUBILITY:	Soluble in DMSO (10mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions are stable for up to 3 months when stored at -20°C.
HANDLING:	Hygroscopic. Packaged under inert gas. Protect from light.
HAZARD:	HARMFUL.
IDENTITY:	Identity determined by ¹H-NMR.

Product Description

Potent and cell permeable ATP competitive JNK inhibitor (hJNK1: IC₅₀=150nM, hJNK2: IC₅₀=220nM and hJNK3: IC₅₀=70 nM). Displays anti-inflammatory properties and has been shown to reduce TNF-α plasma levels induced by LPS in mice.

Product Specific Literature References

AS601245 (1,3-benzothiazol-2-yl (2-[[2-(3-pyridinyl) ethyl] amino]-4 pyrimidinyl) acetonitrile): a c-Jun NH2-terminal protein kinase inhibitor with neuroprotective properties: S. Carboni, et al.; J. Pharmacol. Exp. Ther. 310, 25 (2004) Abstract; Full Text
Design and synthesis of the first generation of novel potent, selective, and in vivo active (benzothiazol-2-yl)acetonitrile inhibitors of the c-Jun N-terminal kinase: P. Gaillard, et al.; J. Med. Chem. 48, 4596 (2005) Abstract

Further Categories Containing This Product:

TNF-alpha & TNF Receptors Other Products • Anti-inflammatory Agents Other Products

ALX-522-025

Revised 25-Aug-08

BAFF, Soluble (human) (recombinant)
SYNONYMS	BlyS, Soluble (human) (recombinant) TALL-1, Soluble (human) (recombinant) THANK, Soluble (human) (recombinant) zTNF4, Soluble (human) (recombinant) TNFSF 13B/20, Soluble (human) (recombinant) CD257, Soluble (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	BAFF

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-025-C010		10 µg	365.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
MW:	~28kDa (SDS-PAGE).
SOURCE/HOST:	Produced in E. coli. The extracellular domain of human BAFF (aa 83-285) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to human and mouse (weak) BCMA, TACI and BAFF-R.
BIOLOGICAL ACTIVITY:	Mediates splenocyte survival. Induces monocyte survival.
APPLICATION:	Detection of receptors of human soluble BAFF on target cells.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Images

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Product Specific Literature References

BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med. 192, 129 (2000) Abstract; Full Text
BAFF mediates survival of peripheral immature B lymphocytes: M. Batten, et al.; J. Exp. Med. 192, 1453 (2000) Abstract; Full Text
Maturation of marginal zone and follicular B cells requires B cell activating factor of the tumor necrosis factor family and is independent of B cell maturation antigen: P. Schneider, et al.; J. Exp. Med. 194, 1691 (2001) Abstract
T cell costimulation by the TNF ligand BAFF: B. Huard, et al.; J. Immunol. 167, 6225 (2001) Abstract
Aberrant expression of B-lymphocyte stimulator by B chronic lymphocytic leukemia cells: a mechanism for survival: A.J. Novak, et al.; Blood 100, 2973 (2002) Abstract; Full Text
BAFF is a survival and maturation factor for mouse B cells: A.G. Rolink, et al.; Eur. J. Immunol. 32, 2004 (2002) Abstract
Expression of BLyS and its Receptors in B-Cell Non-Hodgkin Lymphoma: Correlation with Disease Activity and Patient Outcome: A.J. Novak, et al.; Blood 104, 2247 (2004) Abstract
TACI is mutant in common variable immunodeficiency and IgA deficiency: E. Castigli, et al.; Nat. Genet. 37, 829 (2005) Abstract
A role for BLyS in the activation of innate immune cells: S.K. Chang, et al.; Blood 108, 2687 (2006) Abstract; Full Text
Viral Double-Stranded RNA Triggers Ig Class Switching by Activating Upper Respiratory Mucosa B Cells through an Innate TLR3 Pathway Involving BAFF: W. Xu, et al.; J. Immunol. 181, 276 (2008) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link Q9Y275: BAFF (human)
AfCS Signalling Gateway link A000383: BAFF (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

TNFR-associated factor-3 is associated with BAFF-R and negatively regulates BAFF-R-mediated NF-kappa B activation and IL-10 production: L.G. Xu & H.B. Shu; J. Immunol. 169, 6883 (2002) Abstract; Full Text

Further Categories Containing This Product:

Recombinant Proteins/Fusion Proteins

ALX-522-052

Revised 02-May-07

BAFF, Soluble (mouse) (recombinant)
SYNONYMS	BLyS, Soluble (mouse) (recombinant) TALL-1, Soluble (mouse) (recombinant) THANK, Soluble (mouse) (recombinant) zTNF4, Soluble (mouse) (recombinant) TNFSF 13B/20, Soluble (mouse) (recombinant) CD257, Soluble (mouse) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	BAFF

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-052-C010		10 µg	365.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
MW:	~17kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of mouse BAFF (aa 127-309) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or other carrier protein.
SPECIFICITY:	Binds to human (weak) and mouse BCMA, TACI and BAFF-R.
BIOLOGICAL ACTIVITY:	Mediates splenocyte survival.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Images

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Product Specific Literature References

Act1, a negative regulator in CD40- and BAFF-mediated B cell survival: Y. Qian, et al.; Immunity 21, 575 (2004) Abstract
BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway: A. Craxton, et al.; J. Exp. Med. 202, 1363 (2005) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link Q9WU72: BAFF (mouse)
AfCS Signalling Gateway link A000383: BAFF (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

Further Categories Containing This Product:

Recombinant Proteins/Fusion Proteins

APO-54N-022

Revised 01-Apr-08

BAFF, Soluble (human) Detection Set [For ELISA Application]
SYNONYMS	BLyS, Soluble (human) Detection Set [For ELISA Application] TALL-1, Soluble (human) Detection Set [For ELISA Application] zTNF4, Soluble (human) Detection Set [For ELISA Application] TNFSF 13B/20, Soluble (human) Detection Set [For ELISA Application] CD257, Soluble (human) Detection Set [For ELISA Application]
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	BAFF

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-022-KI01		1 Set	560.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	0.3ng/ml (range 0 to 30ng/ml).
QUANTITY:	For 5x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD) 1 vial Coating Antibody (COAT) 1 vial Detection Antibody (DET) 1 vial Goat anti-rat IgG-HRP (HRP)
APPLICATION:	For the quantitative determination of soluble human BAFF from biological fluids (serum and cell culture supernatant). Does not cross-react with mouse BAFF.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

BAFF (B cell activation factor of the TNF family, also known as BLyS or TALL1) is a cytokine expressed predominantly by cells of the immune system such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells, activated T cells and some malignant B cells. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and also acts in processes such as immunoglobulin isotype switch and B cell co-stimulation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of this membrane-bound protein, which can readily be released in a soluble form, leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q9Y275: BAFF (human)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

The TNF family members BAFF and APRIL: the growing complexity: F. Mackay & C. Ambrose; Cytokine Growth Factor Rev. 14, 311 (2003) Abstract
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol. 172, 812 (2004) Abstract
BAFF Augments Certain Th1-Associated Inflammatory Responses: A.P. Sutherland, et al.; J. Immunol. 174, 5537 (2005) Abstract; Full Text
The BAFF/APRIL system: life beyond B lymphocytes: L.G. Ng, et al.; Mol. Immunol. 42, 763 (2005) Abstract
The BAFF/APRIL system: an important player in systemic rheumatic diseases: F. Mackay, et al.; Curr. Dir. Autoimmun. 8, 243 (2005) Abstract

Further Categories Containing This Product:

ELISA Sets

APO-54N-013

Revised 20-Jun-08

BAFF, Soluble (mouse) Detection Set [For ELISA Application]
SYNONYMS	BLyS, Soluble (mouse) Detection Set [For ELISA Application] TALL-1, Soluble (mouse) Detection Set [For ELISA Application] zTNF4, Soluble (mouse) Detection Set [For ELISA Application] TNFSF 13B/20, Soluble (mouse) Detection Set [For ELISA Application] CD257, Soluble (mouse) Detection Set [For ELISA Application]
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	BAFF

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-013-KI01		1 Set	1'050.00 USD

Product Specification

SENSITIVITY:	0.3ng/ml (range 0 to 20ng/ml)
QUANTITY:	For 4x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of soluble mouse BAFF from biological fluids (serum and cell culture supernatant).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Images

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Product Specific Literature References

Toll-like Receptor 9-Dependent and -Independent Dendritic Cell Activation by Chromatin-Immunoglobulin G Complexes: M.W. Boule, et al.; J. Exp. Med. 199, 1631 (2004) Abstract
Regulation of B-cell survival by BAFF-dependent PKCdelta-mediated nuclear signalling: I. Mecklenbrauker, et al.; Nature 431, 456 (2004) Abstract
BAFF Augments Certain Th1-Associated Inflammatory Responses: A.P. Sutherland, et al.; J. Immunol. 174, 5537 (2005) Abstract; Full Text
A role for B cell-activating factor of the TNF family in chemically induced autoimmunity: Y. Zheng, et al.; J. Immunol. 175, 6163 (2005) Abstract
Development of autoantibody responses in NC/Nga mice: its prevention by pulverized konjac glucomannan feeding: N. Onishi, et al.; Arch. Dermatol. Res. 300, 95 (2008) Abstract

General Information

BAFF (B cell activation factor of the TNF family, also known as BLyS or TALL1) is a cytokine expressed predominantly by cells of the immune system such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells, activated T cells and some malignant B cells. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and also acts in processes such as immunoglobulin isotype switch and B cell co-stimulation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of this membrane-bound protein, which can readily be released in a soluble form, leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link : BAFF (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

Further Categories Containing This Product:

ELISA Sets

ALX-840-605

Revised 09-Jun-05

Positive Control (Cell Lysate) for Antibodies to BAFF (human)
SYNONYMS	Positive Control (Cell Lysate) for Antibodies to BlyS (human) Positive Control (Cell Lysate) for Antibodies to TALL-1 (human) Positive Control (Cell Lysate) for Antibodies to THANK (human) Positive Control (Cell Lysate) for Antibodies to zTNF4 (human) Positive Control (Cell Lysate) for Antibodies to TNFSF 13B/20 (human) Positive Control (Cell Lysate) for Antibodies to CD257 (human)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Positive Controls (Cell Lysates & Extracts)

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-840-605-R100		100 µl	70.00 USD

Product Specification

SOURCE/HOST:	Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human BAFF (aa 83-285).
QUANTITY:	100µl corresponding to 2 x 10⁵ cells.
FORMULATION:	Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION:	Positive control in Western blot for MAb to BAFF (human) (Buffy-1) (Prod. No. ALX-804-128) and the MAb to BAFF (Buffy-2) (Prod. No. ALX-804-131). Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Images

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General Information

MANUFACTURER

Manufactured by Apotech Corporation.

Further Categories Containing This Product:

BAFF

APO-54N-020

Revised 02-Apr-08

BAFF, Soluble (human) ELISA Kit
SYNONYMS	BlyS, Soluble (human) ELISA Kit TALL-1, Soluble (human) ELISA Kit THANK, Soluble (human) ELISA Kit zTNF4, Soluble (human) ELISA Kit TNFSF 13B/20, Soluble (human) ELISA Kit CD257, Soluble (human) ELISA Kit
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	BAFF

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-020-KI01		1 Kit	630.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	0.95ng/ml (range 0 to 128ng/ml)
QUANTITY:	For 96 wells (~80 tests)
APPLICATION:	For the quantitative determination of soluble human BAFF from biological fluids (serum and cell culture supernatant).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C

General Information

BAFF (B cell activating factor belonging to the TNF family, also known as BLyS or TALL1) is a cytokine expressed predominantly by cells of the immune system such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells, activated T cells and some malignant B cells. BAFF binds three distinct receptors (BAFFR, TACI and BCMA) predominantly expressed on B cells, although activated T cells also express BAFFR. BAFF is a master regulator of peripheral B cell survival, and also acts in processes such as immunoglobulin isotype switch and B cell co-stimulation. Beside its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of this membrane-bound protein, which can readily be released in a soluble form by proteolytic cleavage, leads to autoimmune disorders in mice. In the human, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases, such as rheumatoid arthritis (RA), Sjögren’s syndrome (SS) and systemic lupus erythematosus (SLE). BAFF levels are also elevated in patients with multiple myeloma and B-cell chronic lymphoid leukemia (B-CCL).
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q9Y275: BAFF (human)
MANUFACTURER	Manufactured by Apotech Corporation & Immundiagnostik AG.

General Literature References

The BAFF/APRIL system: life beyond B lymphocytes: L.G. Ng, et al.; Mol. Immunol. 42, 763 (2005) Abstract
BAFF is produced by astrocytes and up-regulated in multiple sclerosis lesions and primary central nervous system lymphoma: M. Krumbholz, et al.; J. Exp. Med. 201, 195 (2005) Abstract

Further Categories Containing This Product:

ELISA & EIA Kits

APO-54N-019

Revised 01-Apr-08

BAFF, Soluble (mouse) ELISA Kit
SYNONYMS	BlyS, Soluble (mouse) ELISA Kit TALL-1, Soluble (mouse) ELISA Kit THANK, Soluble (mouse) ELISA Kit zTNF4, Soluble (mouse) ELISA Kit TNFSF 13B/20, Soluble (mouse) ELISA Kit CD257, Soluble (mouse) ELISA Kit
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	BAFF

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-019-KI01		1 Kit	630.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse
SENSITIVITY:	0.2ng/ml (range 0 to 20ng/ml)
QUANTITY:	For 96 wells (~80 tests)
APPLICATION:	For the quantitative determination of soluble mouse BAFF from biological fluids (serum and cell culture supernatant). Does not cross-react with human BAFF.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C

Product Specific Literature References

In vitro and in vivo activation induces BAFF and APRIL expression in B cells: V.T. Chu, et al.; J. Immunol. 179, 5947 (2007) Abstract

General Information

BAFF (B cell activation factor of the TNF family, also known as BLyS or TALL1) is a cytokine expressed predominantly by cells of the immune system such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells, activated T cells and some malignant B cells. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and also acts in processes such as immunoglobulin isotype switch and B cell co-stimulation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of this membrane-bound protein, which can readily be released in a soluble form, leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link : BAFF (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References