ALEXIS Biochemicals: TL1A, Soluble (mouse) (recombinant)

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ALX-522-055

Revised 24-Mar-05

DcR3 (human):Fc (human) (recombinant)
SYNONYMS	Decoy Receptor 3 (human):Fc (human) (recombinant) TNFRSF 6B (human):Fc (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	HVEM & LIGHT / LT-betaR / DcR3 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-055-C050		50 µg	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
MW:	~58kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human DcR3 (decoy receptor 3) (aa 1-246) is fused to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water.
SPECIFICITY:	Binds human LIGHT. Does not bind human FasL.
APPLICATION:	Detection of human LIGHT and TL1A (VEGI) in combination with PAb to human IgG1.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

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Product Specific Literature References

Genomic amplification of a decoy receptor for Fas ligand in lung and colon cancer: R.M. Pitti, et al.; Nature 396, 699 (1998) Abstract
A newly identified member of tumor necrosis factor receptor superfamily (TR6) suppresses LIGHT-mediated apoptosis: K.Y. Yu, et al.; J. Biol. Chem. 274, 13733 (1999) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link O95407: DcR3 (human) (precursor) AfCS Signalling Gateway link A000758: DcR3 (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

Soluble decoy receptor 3 is expressed by malignant gliomas and suppresses CD95 ligand-induced apoptosis and chemotaxis: W. Roth, et al.; Cancer Res. 61, 2759 (2001) Abstract
Modulation of dendritic cell differentiation and maturation by decoy receptor 3: T.L. Hsu, et al.; J. Immunol. 168, 4846 (2002) Abstract

Related Products

ALX-203-004

Control:Fc Fusion Protein (human) (recombinant)

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

APO-54N-029

Revised 02-Apr-08

LIGHT, Soluble (human) Detection Set [For ELISA Application]
SYNONYMS	HVEML, Soluble (human) Detection Set [For ELISA Application] TNFSF 14, Soluble (human) Detection Set [For ELISA Application] CD258, Soluble (human) Detection Set [For ELISA Application]
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	HVEM & LIGHT / LT-betaR / DcR3 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-029-KI01		1 Set	540.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	0.2ng/ml (range 0 to 20ng/ml).
QUANTITY:	5x96 wells
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of human LIGHT (HVEML; TNFSF 14) from biological fluids (serum, plasma and cell culture supernatant).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C

General Information

LIGHT (derived from "homologous to Lymphotoxins, exhibits Inducible expression, and competes with Herpes simplex virus (HSV) Glycoprotein D for Herpes virus entry mediator (HVEM /TR2), a receptor expressed by T lymphocytes")is a 29 kDa type 2 transmembrane protein that belongs to the TNF superfamily [1, 2]. It is expressed in granulocytes, monocytes, platelets, immature dendritic cells and activated T cells. LIGHT is active either as membrane-anchored or secreted (soluble) forms. Three receptors of the TNF superfamily: lymphotoxin receptor (LTR), herpesvirus entry mediator (HVEM), and decoy receptor 3 (DcR3) binds to the ligand. LIGHT is involved in T-cell regulation and dendritic cell maturation [3]. It triggers several signaling pathways such as NF-κB, non-canonical NF-κB, Stat3, ERK5 / BMK1 and JNK [4]. LIGHT is released from platelets and induces a proinflammatory state in vascular endothelial cells [5]. It suppress tumor formation in vivo by inducing apoptotic cell death. Embryonic stem (ES) cells are differentiated in the presence of LIGHT [6]. Mice overexpressing LIGHT spontaneously develop severe autoimmune disease. LIGHT has been reported to increase in Rhumatoid Arthritis (RA) patients and to induce a RANKL dependent and -independent osteoclasts formation [7].
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link O43557: Tumor Necrosis Factor Ligand Superfamily Member 14 (human)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

[1] LIGHT-HVEM signaling and the regulation of T cell-mediated immunity: S.W. Granger & S. Rickert; Cytokine Growth Factor Rev. 14, 289 (2003) Abstract
[2] LIGHT, a new member of the TNF superfamily, and lymphotoxin alpha are ligands for herpesvirus entry mediator: D.N. Mauri, et al. ; Immunity 8, 21 (1998) Abstract
[3] LIGHT, a TNF-like molecule, costimulates T cell proliferation and is required for dendritic cell-mediated allogeneic T cell response: K. Tamada, et al. ; J. Immunol. 164, 4105 (2000) Abstract
[4] LIGHT, a member of the TNF superfamily, activates Stat3 mediated by NIK pathway: N. Nadiminty, et al. ; BBRC 359, 379 (2007) Abstract
[5] Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes: K. Otterdal, et al. ; Blood 108, 928 (2006) Abstract
[6] LIGHT induces differentiation of mouse embryonic stem cells associated with activation of ERK5: G.M. Zou, et al. ; Oncogene 25, 463 (2006) Abstract
[7] LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis: J.R. Edwards, et al. ; Arthritis Rheum. 54, 1451 (2006) Abstract

Further Categories Containing This Product:

ELISA & EIA Kits

ALX-522-018

Revised 23-Nov-07

LIGHT, Soluble (human) (recombinant)
SYNONYMS	HVEML, Soluble (human) (recombinant) TNFSF 14, Soluble (human) (recombinant) CD258, Soluble (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	HVEM & LIGHT / LT-betaR / DcR3 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-018-C010		10 µg	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
MW:	~25kDa (SDS-PAGE)
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human LIGHT (aa 89-240) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to human and mouse LTβR and human HVEM and DcR3.
BIOLOGICAL ACTIVITY:	Increases LNCaP (prostate cancer cell) growth.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Images

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Product Specific Literature References

T cell costimulation by the TNF ligand BAFF: B. Huard, et al.; J. Immunol. 167, 6225 (2001) Abstract
Endogenous association of TRAF2, TRAF3, cIAP1, and Smac with lymphotoxin beta receptor reveals a novel mechanism of apoptosis: J. Kuai, et al.; J. Biol. Chem. 278, 14363 (2003) Abstract; Full Text
A coreceptor interaction between the CD28 and TNF receptor family members B and T lymphocyte attenuator and herpesvirus entry mediator: L.C. Gonzalez, et al.; PNAS 102, 1116 (2005) Abstract; Full Text
LIGHT, a member of the TNF superfamily, activates Stat3 mediated by NIK pathway: N. Nadiminty, et al.; BBRC 359, 379 (2007) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link O43557: LIGHT (human)
AfCS Signalling Gateway link A001406: LIGHT (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-522-034

Revised 27-Mar-06

LTα3, Soluble (human) (recombinant)
SYNONYMS	TNF-β, Soluble (human) (recombinant) TNFSF 1, Soluble (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	TNF-alpha & TNF Receptors Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-034-C010		10 µg	200.00 USD
ALX-522-034-3010	SuperPack	3x10 µg	410.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
MW:	~25kDa (SDS-PAGE).
SOURCE/HOST:	Produced in E. coli. The extracellular domain of human LTα3 (aa 43-205) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to human HVEM and human and mouse TNF-R1 and TNF-R2.
BIOLOGICAL ACTIVITY:	Kills human TNF-R1 positive cells.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles.

General Information

Lymphotoxin-α (LTα, TNF-β) is a 25 kDa type II soluble protein belonging to the TNF family. It is expressed as a trimer (LTα3), or with lymphotoxin-β (LTα1β2 or LTα2β1). LTα3 interacts with 3 different members of the TNF-receptor superfamily: HVEM (Prod. No. ALX-522-017), TNF-R1 (Prod. No. ALX-522-013) and TNF-R2 (Prod. No. ALX-522-014). LTα3 is expressed by activated T and B lymphocytes and is a pro-apoptotic cytokine, a pluripotent mediator of inflammtory and immune defense mechanisms, and moreover it plays an important role in regulating the generation of the peripheral lymphoid system.

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P01374: TNF-β (human) (precursor)
AfCS Signalling Gateway link A001438: TNF-β (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins • HVEM & LIGHT / LT-betaR / DcR3 / Related Products

ALX-522-041

Revised 20-Jul-06

LTβR (human):Fc (human) (recombinant)
SYNONYMS	Tumor Necrosis Factor Receptor 2-related Protein TNFRSF 3 (human):Fc (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	HVEM & LIGHT / LT-betaR / DcR3 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-041-C050		50 µg	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
MW:	Migrates as a doublet at ~56kDa and ~60kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The cysteine-rich region of human LTβR (aa 1-220) is fused to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute in 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds human and mouse LIGHT and LTα1β2.
BIOLOGICAL ACTIVITY:	Inhibits human LIGHT- and LTα1β2-mediated effects such as cytotoxic effects on HT29 target cells.
APPLICATION:	Detection of human membrane-bound LIGHT in combination with PAb to human IgG1.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Specific Literature References

The lymphotoxin-β receptor is necessary and sufficient for LIGHT-mediated apoptosis of tumor cells: I.A. Rooney, et al.; J. Biol. Chem. 275, 14307 (2000) Abstract; Full Text
Lymphotoxin-mediated regulation of gammadelta cell differentiation by alphabeta T cell progenitors: B. Silva-Santos, et al.; Science 307, 925 (2005) Abstract
Interactions of tumor necrosis factor (TNF) and TNF receptor family members in the mouse and human: C. Bossen, et al.; J. Biol. Chem. 281, 13964 (2006) Abstract; Full Text

General Information

LTβR is a 75kDa type I transmembrane protein belonging to the TNF receptor family. It is 414 aa long and contains 4 cysteine-rich repeats in its extracellular domain. It is expressed on monocytes, fibroblasts, stromal and muscle cells. It is known to activate nuclear factor NF-κB, to interact with NIK and to induce cell death via TNF receptor-associated factor 3 (TRAF3). LTβR has two known ligands: LIGHT and LTα1β2. It has been shown to be involved in splenic architecture development, lymph node genesis and affinity maturation, as well as in the development of diabetes and colitis. It has been implicated in the induction of HIV replication and impairs prion replication in mice lacking follicular dendritic cells.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P36941: LTβR (human) (precursor) AfCS Signalling Gateway link A001440: LTβR (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

HVEM (TR2, ATAR, HVEA) is a 36 kDa type I transmembrane protein belonging to the TNF receptor family. HVEM contains 3 cysteine-rich repeats in the extracellular portion which is typical of TNFR family members. HVEM mediates Herpes Simplex Virus-1 infection of T cells. Ligation of HVEM by its cellular trimeric ligand LIGHT (Prod. No. ALX-522-018) induces recruitment to the cytoplasmic domain of the signaling molecules TRAF2 and 5. Activation in vitro of HVEM leads to induction of nuclear factors NFκB and AP-1. The outcome of HVEM activation on T cells is still controversial. A second ligand for HVEM is soluble LTα.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q92956: HVEM (human) (precursor) AfCS Signalling Gateway link A001150: HVEM (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

SPECIES CROSSREACTIVITY:	Human
CLONE:	37A565
ISOTYPE:	Mouse IgG
CONCENTRATION:	0.5mg/ml
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human DcR3 (decoy receptor 3).
SPECIFICITY:	Recognizes human DcR3. Detects a band of ~34kDa by Western blot.
APPLICATION:	Western Blot (2-4µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 6 months when stored at +4°C.