ALEXIS Biochemicals: Thy-1 (RLE) (mouse):Fc (human) (recombinant)

ALX-522-097

Revised 27-Mar-08

Thy-1 (RLE) (mouse):Fc (human) (recombinant)
SYNONYMS	CD90 (RLE) (mouse):Fc (human) (recombinant)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	CD Proteins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-097-C050		50 µg	365.00 USD

Product Specification

MW:	~48kDa (SDS-PAGE)
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of mouse Thy-1 (aa 20-131) is fused to the Fc portion of human IgG1. The integrin binding sequence RLD of mouse Thy-1 has been changed to RLE abolishing the binding of integrins.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

General Information

Thy-1 is a glycosyl phosphatidylinositol (GPI)-anchored glycoprotein of the immunoglobulin superfamily expressed in various cell types, particularly in cells of the neuronal system, activited microvascular endothelial cells, fibroblasts and blood stem cells. Thy-1 is involved in cell adhesion. Thy-1 interacts with the integrin avB3 (CD51/CD61) and Mac-1 (CD11b/CD18). In astrocytes, binding of Thy-1 to β3-integrin promotes focal adhesion formation.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P01831: Thy-1 (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

Thy-1 binds to integrin beta(3) on astrocytes and triggers formation of focal contact sites: L. Leyton, et al.; Curr. Biol. 11, 1028 (2001) Abstract
Human Thy-1 (CD90) on activated endothelial cells is a counterreceptor for the leukocyte integrin Mac-1 (CD11b/CD18): A. Wetzel, et al.; J. Immunol. 172, 3850 (2004) Abstract; Full Text
Interaction of human Thy-1 (CD 90) with the integrin alphavbeta3 (CD51/CD61): an important mechanism mediating melanoma cell adhesion to activated endothelium: A. Saalbach, et al.; Oncogene 24, 4710 (2005) Abstract