ALEXIS Biochemicals: U-73343

ALX-370-006

Revised 03-Jun-08

U-73343
SYNONYMS	1-[6-[[(17β)-3-Methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-pyrrolidine-2,5-dione
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	PLA2 Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-370-006-M001		1 mg	20.00 USD
ALX-370-006-M005		5 mg	80.00 USD

Product Specification

FORMULA:	C₂₉H₄₂N₂O₃
MW:	466.7
CAS NUMBER:	142878-12-4
PURITY:	≥98% (TLC)
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in methylene chloride or chloroform; slightly soluble in DMSO or 100% ethanol; sparingly soluble in aqueous solutions.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+20°C
USE/STABILITY:	Stock solutions in DMSO or ethanol are stable for 4 months when stored at -20°C.

Product Description

Analog of U-73122 (Prod. No. ALX-370-005) which only slightly inhibits phospholipase C. Useful as a negative control.

Product Specific Literature References

Receptor-coupled signal transduction in human polymorphonuclear neutrophils: effects of a novel inhibitor of phospholipase C-dependent processes on cell responsiveness: R.J. Smith, et al.; J. Pharmacol. Exp. Ther. 253, 688 (1990) Abstract
Gonadotropin-releasing hormone-stimulated intracellular Ca2+ fluctuations and luteinizing hormone release can be uncoupled from inositol phosphate production: B.E. Hawes, et al.; Endocrinology 130, 3475 (1992) Abstract
A new, nongenomic estrogen action: the rapid release of intracellular calcium: P Morley, et al.; Endocrinology 131, 1305 (1992) Abstract
U-73122, a phospholipase C antagonist, inhibits effects of endothelin-1 and parathyroid hormone on signal transduction in UMR-106 osteoblastic cells: A. Tatrai, et al.; Biochim. Biophys. Acta 1224, 575 (1994) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Preparation of Working Solutions
Compound is only sparingly soluble in aqueous media and may be delivered to cells either after dissolving in organic solvents or complexing with serum proteins. Dissolve a weighed amount in methylene chloride or chloroform. Dispense in aliquots sufficient for one experiment into suitable vessels. Evaporate the solvent with a stream of nitrogen. This leaves a thin, dry film, which may be easily dissolved in DMSO (~5mM) or 100% ethanol (~2mM). As an alternative, an aqueous medium that contains serum or serum albumin (1-10mg/ml) may be used to dissolve the thin film.