ALEXIS Biochemicals: UNC5B (human):Fc (human) (recombinant)

ALX-522-095

Revised 14-Apr-08

UNC5B (human):Fc (human) (recombinant)
SYNONYMS	UNC-5 Homolog B (human):Fc (human) (recombinant) UNC5H2 (human):Fc (human) (recombinant) UNC-5 Homolog 2 (human):Fc (human) (recombinant) p53-regulated Receptor For Death And Life Protein 1 (human):Fc (human) (recombinant) P53RDL1 (human):Fc (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Netrins & Receptors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-095-C050		50 µg	365.00 USD

Product Specification

MW:	~80kDa (SDS-PAGE)
SOURCE/HOST:	Produced in CHO cells. The extracellular domain of human UNC5B (UNC5H2) (aa 28-363) is fused to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds to human, mouse and chicken netrin-1 and to chicken netrin-2.
BIOLOGICAL ACTIVITY:	Inhibits netrin-1 mediated survival of HEK 293 cells overexpressing DCC or UNC5B.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Specific Literature References

Netrin-1 expression confers a selective advantage for tumor cell survival in metastatic breast cancer: J. Fitamant, et al.; PNAS 105, 4850 (2008) Abstract

General Information

The receptors of the UNC family (for uncoordinated) include UNC-5 of C. elegans and its four vertebrate homologs, UNC5A, B, C and D (previously called UNC5H1-4). The UNC5 family binds to the netrin family of ligands and mediates axon repulsion effect of netrin, either alone or by binding to the DCC (another netrin receptor). These receptors are type I transmembrane proteins of roughly 110-120kDa composed of two extracellular Ig-like domains, two thrombospondin type I domains, and intracellular sequence with a ZU5 domain and a death domain. UNC5B is a direct target gene for p53 that mediates p53-dependent apoptosis, and is also a dependence receptor that regulates apoptosis either positively or negatively depending on its binding with netrin-1. UNC5B is highly expressed in brain, but also expressed at lower level in developing lung, cartilage, kidney, hematopoietic and immune tissues, as well as in the vascular system. Unc5B (as well as the other UNC5 receptors) is a putative tumour suppressor because of its frequent alteration and reduced expression in various human cancers. Recently UNC5B has been shown to mediate guidance events controlling the morphogenesis of the vascular system.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q8IZJ1: UNC5B (human)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

The netrin-1 receptors UNC5H are putative tumor suppressors controlling cell death commitment: K. Thiebault, et al.; PNAS 100, 4173 (2003) Abstract
Netrin-1 and its receptors in tumorigenesis: H. Arakawa; Nat. Rev. Cancer 4, 978 (2004) Abstract
The netrin receptor UNC5B mediates guidance events controlling morphogenesis of the vascular system: X. Lu, et al.; Nature 432, 179 (2004) Abstract
p53, apoptosis and axon-guidance molecules: H. Arakawa; Cell Death Differ. 12, 1057 (2005) Abstract

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Recombinant Proteins / Fusion Proteins

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