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ALX-850-296 Revised 26-Sep-08
Leptin Receptor (human) ELISA Kit
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SYNONYMS OB-R (human) ELISA Kit
CD295 (human) ELISA Kit
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Leptin / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-296-KI01   1 Kit 696.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 0.4ng/ml (range 2 to 100ng/ml)
QUANTITY: For 96 wells (~80 tests)
APPLICATION: For the quantitative determination of human leptin receptor in serum, plasma and cell culture supernatant. Weakly cross-reacts with mouse, dog, horse and pig leptin receptor. Does not cross-react with rat serum.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 6 months when stored at +4°C.
Product Specific Literature References
Regulation of circulating soluble leptin receptor levels by gender, adiposity, sex steroids, and leptin: observational and interventional studies in humans: J.L. Chan, et al.; Diabetes 51, 2105 (2002) Abstract
Mutations in the human leptin and leptin receptor genes as models of serum leptin receptor regulation: N. Lahlou, et al.; Diabetes 51, 1980 (2002) Abstract
Weight loss increases soluble leptin receptor levels and the soluble receptor bound fraction of leptin: M. Laimer, et al.; Obes. Res. 10, 597 (2002) Abstract
Soluble leptin receptor and insulin resistance as determinant of sleep apnea: D. Manzella, et al.; Int. J. Obes. Relat. Metab. Disord. 26, 370 (2002) Abstract
Opposite modifications in circulating leptin and soluble leptin receptor across the eating disorder spectrum: P. Monteleone, et al.; Mol. Psychiatry 7, 641 (2002) Abstract
Obesity is associated with decreasing levels of the circulating soluble leptin receptor in humans: V. Ogier, et al.; Int. J. Obes. Relat. Metab. Disord. 26, 496 (2002) Abstract
Soluble leptin receptors and serum leptin in end-stage renal disease: relationship with inflammation and body composition: R. Pecoits-Filho, et al.; Eur. J. Clin. Invest. 32, 811 (2002) Abstract
Circulating concentrations of soluble leptin receptor: influence of menstrual cycle and diet therapy: H. Shimizu, et al.; Nutrition 18, 309 (2002) Abstract
Leptin and soluble leptin receptor levels in obese and weight-losing individuals: F.M. van Dielen, et al.; J. Clin. Endocrinol. Metab. 87, 1708 (2002) Abstract
Leptin, soluble leptin receptor, and transforming growth factor-beta1 levels in minimal change nephrotic syndrom: N. Buyan, et al.; Pediatr. Nephrol. 18, 1009 (2003) Abstract
Diurnal and ultradian dynamics of serum adiponectin in healthy men: comparison with leptin, circulating soluble leptin receptor, and cortisol patterns: A. Gavrila, et al.; J. Clin. Endocrinol. Metab. 88, 2838 (2002) Abstract; Full Text
Relationships between the serum levels of soluble leptin receptor and free and bound leptin in non-pregnant women of reproductive age and women undergoing controlled ovarian hyperstimulation: N. Kado, et al.; Hum. Reprod. 18, 715 (2003) Abstract; Full Text
Changes in circulating leptin, leptin receptor, and gonadal hormones from infancy until advanced age in humans: D.R. Mann, et al.; J. Clin. Endocrinol. Metab. 88, 3339 (2003) Abstract; Full Text
Serum ghrelin levels in obese patients: the relationship to serum leptin levels and soluble leptin receptors levels: M. Rosicka, et al.; Physiol. Res. 52, 61 (2003) Abstract; Full Text
Soluble leptin receptor and soluble receptor-bound fraction of leptin in the metabolic syndrome: A. Sandhofer, et al.; Obes. Res. 11, 760 (2003) Abstract; Full Text
Body fat mass and macronutrient intake in relation to circulating soluble leptin receptor, free leptin index, adiponectin, and resistin concentrations in healthy humans: M. Yannakoulia, et al.; J. Clin. Endocrinol. Metab. 88, 1730 (2003) Abstract; Full Text
Leptin and its soluble receptor in plasma of patients suffering from remitting-relapsing multiple sclerosis (MS) In vitro effects of leptin on type-1 and type-2 cytokine secretion by peripheral blood mononuclear cells, T-cells and monocytes of MS patients: K. Chatzantoni, et al.; J. Autoimm. 23, 169 (2004) Abstract
Hormonal and body composition predictors of soluble leptin receptor, leptin, and free leptin index in adolescent girls with anorexia nervosa and controls and relation to insulin sensitivity: M. Misra, et al.; J. Clin. Endocrinol. Metab. 89, 3486 (2004) Abstract; Full Text
Serum leptin, soluble leptin receptor, free leptin index and bone mineral density in patients with primary biliary cirrhosis: F. Szalay, et al.; Eur. J. Gastroenterol. Hepatol. 17, 923 (2005) Abstract
Expression of leptin receptors in mononuclear cells from myelodysplastic syndromes and acute myeloid leukemias: P.C. Tsiotra, et al.; Acta Haematol. 114, 71 (2005) Abstract
General Information
MANUFACTURER Manufactured by BioVendor.
General Literature References
Identification and expression cloning of a leptin receptor: L.A. Tartaglia, et al.; Cell 83, 1263 (1995) Abstract
Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice: H. Chen, et al.; Cell 84, 491 (1996) Abstract
Novel B219/OB receptor isoforms: possible role of leptin in hematopoiesis and reproduction: J.A. Cioffi, et al.; Nat. Med. 2, 585 (1996) Abstract
Abnormal splicing of the leptin receptor in diabetic mice: G.H. Lee; Nature 379, 632 (1996) Abstract
The leptin receptor: L.A. Tartaglia; J. Biol. Chem. 272, 6093 (1997) Abstract
Leptin: J. Auwerx & B. Staels; Lancet 351, 737 (1998), (Review) Abstract
Leptin and its receptors: regulators of whole-body energy homeostasis: K.L. Houseknecht & C.P. Portocarrero; Domest. Anim. Endocrinol. 15, 457 (1998) Abstract
Further Categories Containing This Product:
CD Kits & Sets • ELISA & EIA Kits
 
 
APO-54N-029 Revised 05-Dec-08
LIGHT, Soluble (human) Detection Set [For ELISA Application]
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SYNONYMS HVEML, Soluble (human) Detection Set [For ELISA Application]
TNFSF 14, Soluble (human) Detection Set [For ELISA Application]
CD258, Soluble (human) Detection Set [For ELISA Application]
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY HVEM & LIGHT / LT-betaR / DcR3 / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
APO-54N-029-KI01   1 Set 540.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 0.2ng/ml (range 0 to 20ng/ml).
QUANTITY: 5x96 wells
KIT/SET CONTAINS: 1 vial Standard (lyophilized) (STD).
1 vial Coating Antibody (COAT).
1 vial Detection Antibody (DET).
APPLICATION: For the quantitative determination of human LIGHT (HVEML; TNFSF 14) from biological fluids (serum, plasma and cell culture supernatant).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
General Information
LIGHT (derived from "homologous to Lymphotoxins, exhibits Inducible expression, and competes with Herpes simplex virus (HSV) Glycoprotein D for Herpes virus entry mediator (HVEM /TR2), a receptor expressed by T lymphocytes")is a 29 kDa type 2 transmembrane protein that belongs to the TNF superfamily [1, 2]. It is expressed in granulocytes, monocytes, platelets, immature dendritic cells and activated T cells. LIGHT is active either as membrane-anchored or secreted (soluble) forms. Three receptors of the TNF superfamily: lymphotoxin receptor (LTR), herpesvirus entry mediator (HVEM), and decoy receptor 3 (DcR3) binds to the ligand. LIGHT is involved in T-cell regulation and dendritic cell maturation [3]. It triggers several signaling pathways such as NF-κB, non-canonical NF-κB, Stat3, ERK5 / BMK1 and JNK [4]. LIGHT is released from platelets and induces a proinflammatory state in vascular endothelial cells [5]. It suppress tumor formation in vivo by inducing apoptotic cell death. Embryonic stem (ES) cells are differentiated in the presence of LIGHT [6]. Mice overexpressing LIGHT spontaneously develop severe autoimmune disease. LIGHT has been reported to increase in Rhumatoid Arthritis (RA) patients and to induce a RANKL ­dependent and -independent osteoclasts formation [7].
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link O43557: Tumor Necrosis Factor Ligand Superfamily Member 14 (human)
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] LIGHT-HVEM signaling and the regulation of T cell-mediated immunity: S.W. Granger & S. Rickert; Cytokine Growth Factor Rev. 14, 289 (2003) Abstract
[2] LIGHT, a new member of the TNF superfamily, and lymphotoxin alpha are ligands for herpesvirus entry mediator: D.N. Mauri, et al. ; Immunity 8, 21 (1998) Abstract
[3] LIGHT, a TNF-like molecule, costimulates T cell proliferation and is required for dendritic cell-mediated allogeneic T cell response: K. Tamada, et al. ; J. Immunol. 164, 4105 (2000) Abstract
[4] LIGHT, a member of the TNF superfamily, activates Stat3 mediated by NIK pathway: N. Nadiminty, et al. ; BBRC 359, 379 (2007) Abstract
[5] Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes: K. Otterdal, et al. ; Blood 108, 928 (2006) Abstract
[6] LIGHT induces differentiation of mouse embryonic stem cells associated with activation of ERK5: G.M. Zou, et al. ; Oncogene 25, 463 (2006) Abstract
[7] LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis: J.R. Edwards, et al. ; Arthritis Rheum. 54, 1451 (2006) Abstract
Further Categories Containing This Product:
ELISA & EIA Kits
 
 
AG-L0441 Revised 14-Feb-07
Listeria monocytogenes SURE mono ELISA Kit
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PRODUCT LINE Immunology
PRODUCT CATEGORY Bacteria Other Products
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AG-L0441EK-KI01   96 wells 490.00 USD Add To Cart
Product Specification
SENSITIVITY: 1ng/ml culture supernatant.
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the qualitative determination of L. monocytogenes.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
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Product Description

The genus Listeria is comprised of six species: L. monocytogenes, L. ivanovii, L. innocua, L. welshimeri, L. seeligeri, and L. grayi. All Listeria species are widely found in nature as well as in many food industry related applications. Among these species, L. monocytogenes is known to infect humans, causing sepsis, meningitis, or encephalitis. This organism can be classified as an important opportunistic infectious agent which is becoming an emerging problem in public hygiene. In order to prevent L. monocytogenes infection, prescreening of processed food with a reliable diagnostic kit is necessary.

The AdipoGen Listeria monocytogenes SURE mono ELISA Kit is an easy, ready-to-use system to specifically identify L. monocytogenes. The system relies on the outstanding mononclonal capture-antibody p6007 (Prod. No. ALX-804-705) that recognizes specifically the secretory enzyme p60 of L. monocytogenes and a polyclonal antibody recognizing pan p60 (Prod. No. ALX-210-415) as the secondary antibody. p6007 discriminates L. monocytogenes from the other Listeria species.
Product Specific Literature References
Use of monoclonal antibodies that recognize p60 for identification of Listeria monocytogenes: K.Y. Yu, et al.; Clin. Diag. Lab. Immunol. 11, 446 (2004) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Please also see our Product Flyer "Specific Detection of Listeria monocytogenes".
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
ELISA & EIA Kits
 
 
ALX-850-270 Revised 15-Feb-07
M30-Apoptosense® ELISA Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Cytokeratin-based Apoptosis Detection
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-270-KI01 (CE Mark) 1 Kit Inquire
ALX-850-270-5001 (CE Mark) 5x1 Kit Inquire
Product Specification
APPLICATION: For the specific and sensitive detection of caspase-cleaved intermediate filament protein cytokeratin-18 (CK18Asp396-NE: M30 neo-epitope). The M30-Apoptosense® ELISA is primarely intended for quantitative determination of epithelial apoptosis in cytosolic cell extracts or cell culture supernatants, human serum or plasma or other body fluids.
Use in combination with the M65® ELISA Kit (Prod. No. ALX-850-310) to allow the calculation of apoptosis:necrosis ratios.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Do not freeze.
Product Specific Literature References
Immunocytochemical detection and mapping of a cytokeratin 18 neo-epitope exposed during early apoptosis: M.P. Leers, et al.; J. Pathol. 187, 567 (1999) Abstract
Cisplatin induces the proapoptotic conformation of Bak in a deltaMEKK1-dependent manner: A. Mandic, et al.; Mol. Cell. Biol. 21, 3684 (2001) Abstract
An immunohistochemical study of the clearance of apoptotic cellular fragments: M.P. Leers, et al.; Cell. Mol. Life Sci. 59, 1358 (2002) Abstract
A novel high-through-put assay for screening of pro-apoptotic drugs: M. Hagg, et al.; Invest. New Drugs 20, 253 (2002) Abstract
Measurement of an apoptotic product in the sera of breast cancer patients: T. Ueno, et al.; Eur. J. Cancer 39, 769 (2003) Abstract
A novel assay for discovery and characterization of pro-apoptotic drugs and for monitoring apoptosis in patient sera: K. Biven, et al.; Apoptosis 8, 263 (2003) Abstract
Betulinic acid: a new cytotoxic compound against malignant head and neck cancer cells: D. Thurnher, et al.; Head and Neck 25, 732 (2003) Abstract
Differentiation between cell death modes using measurements of different soluble forms of extracellular cytokeratin 18: G. Kramer, et al.; Cancer Res. 64, 1751 (2004) Abstract
Keratin 8/18 breakdown and reorganization during apoptosis: B. Schutte, et al.; Exp. Cell Res. 297, 11 (2004) Abstract
Validation of pharmacodynamic assays to evaluate the clinical efficacy of an antisense compound (AEG 35156) targeted to the X-linked inhibitor of apoptosis protein XIAP: J. Cummings, et al.; Br. J. Cancer 92, 532 (2005) Abstract
Apoptotic cytokeratin 18 neoepitopes in serum of patients with chronic hepatitis C: B. Kronenberger, et al.; J. Viral Hepat. 12, 307 (2005) Abstract
Expression of the antiapoptotic gene seladin-1 and octreotide-induced apoptosis in growth hormone-secreting and nonfunctioning pituitary adenomas: P. Luciani, et al.; J. Clin. Endocrinol. Metab. 90, 6156 (2005) Abstract; Full Text
The extent of liver steatosis in chronic hepatitis C virus infection is mirrored by caspase activity in serum: N. Seidel, et al.; Hepatology 42, 113 (2005) Abstract
For an extensive and regularly updated literature list, please click here.:
General Information
BACKGROUND/TECHNICAL INFORMATION

Cytokeratin-18 expression has been shown in but is not limited to cells and tissues from lung, breast, intestine, gut, colon, kidney, pancreas, bladder, trophoblasts.
The M30‑Apoptosense® ELISA uses the well-established M30 CytoDEATH monoclonal antibody which has been shown in numerous independent studies to be specific to the CK18 Asp396 caspase cleavage site and represents a validated apoptosis marker that does not react with viable or necrotic cells.

Apoptosis detection using M30 CytoDEATH has been described in several publications to serve as a superior technique compared to TUNEL or detection of gammaH2AX phosphorylation. Assessment of apoptosis by immunohistochemical markers compared to cellular morphology in ex vivo‑stressed colonic mucosa under conditions of DNA-damage may be repaired and results in false-positive counts of apoptotic cells. In addition has modest or transient caspase activation without the cleavage of death substrates been attributed to non-suicidal processes such as proliferation, differentiation and inflammation.

The unmatched sensitivity and superior performance characteristics of M30‑Apoptosense® ELISA even allows the tracing of apoptotic epithelial cells (e.g. carcinoma cells) in human blood samples as no contribution comes from apoptotic inflammatory lymphoid cells as those belong -together with neuronal cells and tissues- to the prominent populations of Cytokeratin-18 negative cells.

By measuring the caspase-cleaved Cytokeratin-18 with M30 one can selectively quantify the extent of non-lympoid e.g. epithelial carcinoma target cells in natural killer (NK) activity tests in one well containing a mixed cell population of otherwise un-labeled effector (T cells: CK18 and M30 negative) and target cells (carcinoma cells: CK18 and M30 positive).

As the principle of the M30‑Apoptosense® ELISA is based upon measuring the rapid accumulation of a caspase cleavage product -that is comparably stable- rather than proteolytic activity itself, it is recommended as an advantageous assay for high through‑put screening (HTS) selective for pro-apoptotic compounds:
- It avoids the necessity to analyse multiple time‑points after the apoptotic stimulus as it has the broadest time-window for optimal signal strength compared to alternative apoptosis detection methods as it does not rely on cell proliferation M30‑Apoptosense® ELISA avoids false positive counts due to cell cycle arrest, senescence or differentiation.
- For mechanistically studies the optional analysis of only the cell culture supernatant avoiding cell lysis allows the ongoing manipulation of the cells to study sensitization or synergistic effects of different stimuli (e.g. drugs) in the same tissue culture well.

Please also see our Product Flyer "M30 CytoDEATH™ - Key Marker to Detect Apoptosis in Epithelial Cells" and the Original Manufacturer’s Product Description.
MANUFACTURER Manufactured by Peviva AB.
Related Products
Further Categories Containing This Product:
ELISA & EIA Kits • Tumor Markers
 
 
ALX-850-310 Revised 04-Jul-08
M65® ELISA Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Cytokeratin-based Apoptosis Detection
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-310-KI01 (CE Mark) 1 Kit Inquire
Product Specification
APPLICATION: For the specific and sensitive detection of soluble CK18 (cytokeratin 18). The M65® ELISA is primarily intended for quantitative determination of total cell death (apoptosis and necrosis) of CK18-positive cells (e.g. carcinoma) in human serum and plasma samples.
Use in combination with the M30-Apoptosense® ELISA Kit (Prod. No. ALX-850-270) to allow the calculation of apoptosis:necrosis ratios.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Do not freeze.
Product Specific Literature References
Differentiation between cell death modes using measurements of different soluble forms of extracellular cytokeratin 18: G. Kramer, et al.; Cancer Res. 64, 1751 (2004) Abstract
Determining tumor apoptosis and necrosis in patient serum using cytokeratin 18 as a biomarker: S. Linder, et al.; Cancer Lett. 214, 1 (2004) Abstract
Apoptosis pathway-targeted drugs--from the bench to the clinic: J. Cummings, et al; Biochim. Biophys. Acta 1705, 53 (2004) Abstract
Induction of lysosomal membrane permeabilization by compounds that activate p53-independent apoptosis: H. Erdal, et al.; PNAS 102, 192 (2005) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Please also see our Product Flyer "M30 CytoDEATH™ - Key Marker to Detect Apoptosis in Epithelial Cells" (click here to download pdf) and the Original Manufacturer’s Product Description.
MANUFACTURER Manufactured by Peviva AB.
Related Products
Further Categories Containing This Product:
ELISA & EIA Kits • Tumor Markers
 
 
APO-54N-039 Revised 10-Oct-08 New product
MDA5 (human) (IntraCellular) ELISA Kit
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SYNONYMS Melanoma Differentiation-associated Gene 5 (human) (IntraCellular) ELISA Kit
Helicard (human) (IntraCellular) ELISA Kit
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
Ordering Information
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APO-54N-039-KI01   1 Kit 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 0.4ng/ml (range 0.5 to 32ng/ml).
QUANTITY: For 96 wells (~80 tests).
APPLICATION: For the quantitative determination of human MDA5 in cell extracts.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
NF-kB Pathway Other Products • Antiviral Signalling / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • ELISA & EIA Kits
 
 
ALX-850-319 Revised 30-Sep-08
Microcystins (Adda specific) ELISA Kit
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Microcystins
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ALX-850-319-KI01   1 Kit 575.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.1ng/ml (range 0.15 to 5ng/ml)
QUANTITY: 96 wells (~80 tests)
APPLICATION: For the quantitative and sensitive congener-independent detection of microcystins and nodularin in water samples.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
Product Description
Polyclonal antibody prepared against the Adda moiety binds to microcystins and nodularins, allowing the congener-independent determination of these toxins and many of its congeners, and does not cross-react with other non-related toxins or compounds. No pre-sample preparation required. Total time for measurement is less than 2.5 hours. Enables simultaneous measurement of multiple samples at reasonable costs. Patented technology.
U.S. Patent No. 6,967,240
Worldwide Patent PCT WO 01/18059 A2
Product Specific Literature References
Congener-independent immunoassay for microcystins and nodularins: W.J. Fischer, et al.; Environ. Sci. Technol. 35, 4849 (2001) Abstract
A review of analytical methods for assessing the public health risk from microcystin in the aquatic environment: P.R. Hawkins, et al.; J. Water Supply 54, 509 (2005)
General Information
Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa.
MANUFACTURER Manufactured by Abraxis LLC.
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Further Categories Containing This Product:
ELISA & EIA Kits • Marine Natural Products
 
 
ALX-850-324 Revised 30-Sep-08
Microcystins-DM ELISA Kit
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Microcystins
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-324-KI01   1 Kit 575.00 USD