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Apoptosis & Necrosis Detection
You are here: Product Lines > Cell Death / Apoptosis / Autophagy > Apoptosis & Necrosis Detection
 
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ALX-850-043 Revised 27-Jul-05
ABSOLUTE-S™ Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY DNA-based Apoptosis Detection
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ALX-850-043-KI01   1 Kit 540.00 USD Add To Cart
Product Specification
QUANTITY: 50 assays.
KIT/SET CONTAINS: Contains all the reagents necessary, including positive and negative control cells.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -20°C
POSITIVE CONTROL INCLUDED: yes
Product Description
The kit utilizes the SBIP™ (strand break induced photolysis) methodology in which cells are irradiated with UV light to induce strand breaks. Does not require DNA denaturation and, therefore, is applicable in studies where preservation of antigens or other features of the cells is desired.
Product Specific Literature References
Detection of 5-bromo-2-deoxyuridine incorporated into DNA by labeling strand breaks induced by photolysis (SBIP): X. Li, et al.; Int. J. Oncol. 4, 1157 (1994)
Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color: X. Li, et al.; Exp. Cell. Res. 222, 28 (1996) Abstract
General Information
MANUFACTURER Manufactured by Phoenix Flow Systems.
Further Categories Containing This Product:
Detection Kits
 
 
ALX-380-283 Revised 21-May-08
7-Amino-actinomycin D
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SYNONYMS 7-AAD
7-Aminoactinomycin C1
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antitumor Antibiotics
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-380-283-M001   1 mg 90.00 USD Add To Cart
ALX-380-283-M005   5 mg 360.00 USD Add To Cart
Product Specification
FORMULA: C62H87N13O16
MW: 1270.4
CAS NUMBER: 7240-37-1
RTECS: AU1579000
SOURCE/HOST: Semisynthetic from actinomycin D (Prod. No. ALX-380-009).
PURITY: ≥96% (HPLC)
APPEARANCE: Red to dark purple powder.
SOLUBILITY: Soluble in DMSO (20mg/ml), methanol or 100% ethanol; slightly soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light. Hygroscopic.
HAZARD: VERY TOXIC.

Product Description
Membrane impermeable fluorescent DNA intercalator. Used as a fluorescent DNA stain. Inhibitor of DNA-primed RNA polymerase and DNA polymerase. Induces apoptosis and is used as a tool to identify apoptotic cells. Cytochemical probe. Exhibits growth-inhibitory activity against certain leukemias and sarcomas. Has antibacterial properties.
Product Specific Literature References
7-Amino-actinomycin D as a cytochemical probe. I. Spectral properties: J.E. Gill, et al.; J. Histochem. Cytochem. 23, 793 (1975) Abstract; Full Text
7-substituted actinomycin D analogs. Chemical and growth-inhibitory studies: S.K. Sengupta, et al.; J. Med. Chem. 18, 1175 (1975) Abstract
Simultaneous cell cycle analysis and two-color surface immunofluorescence using 7-amino-actinomycin D and single laser excitation: applications to study of cell activation and the cell cycle of murine Ly-1 B cells: P.S. Rabinovitch, et al.; J. Immunol. 136, 2769 (1986) Abstract
Binding specificities of actinomycin D to non-self-complementary -XGCY-tetranucleotide sequences: F.M. Chen; Biochemistry 31, 6223 (1992) Abstract
Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry: I. Schmid, et al.; Cytometry 13, 204 (1992) Abstract
Improved staining method for the simultaneous flow cytofluorometric analysis of DNA content, S-phase fraction, and surface phenotype using single laser instrumentation: K. Toba, et al.; Cytometry 13, 60 (1992) Abstract
Flow cytometric immunofluorescence assay for quantification of cyclobutyldithymine dimers in separate phases of the cell cycle: R.J. Berg, et al.; Carcinogenesis 14, 103 (1993) Abstract
Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y: K. Toba, et al.; J. Immunol. Methods 182, 193 (1995) Abstract
Hematopoietic cell protein-tyrosine phosphatase-deficient motheaten mice exhibit T cell apoptosis defect: X. Su, et al.; J. Immunol. 156, 4198 (1996) Abstract
The use of 7-amino actinomycin D in identifying apoptosis: simplicity of use and broad spectrum of application compared with other techniques: N.J. Philpott, et al.; Blood 87, 2244 (1996) Abstract; Full Text
A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry: I. Schmid, et al.; J. Immunol. Methods 170, 145 (1996) Abstract
Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods: H. Lecoeur, et al.; J. Immunol. Methods 209, 111 (1997) Abstract
Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia using 7AAD/PY: K. Toba, et al.; Eur. J. Haematol. 64, 10 (2000) Abstract
Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D: I. Schmid, et al.; Nat. Protoc. 2, 187 (2007) Abstract
Related Products
Further Categories Containing This Product:
Antibiotics - Apoptosis Inducers & Inhibitors • Apoptosis & Necrosis Detection Other Products • DNA Intercalators & Crosslinkers • Transcription Inhibitors / Related Products • Antitumor Agents (Apoptosis Inducers) • Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-201-116 Revised 21-Jan-08
Annexin V (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-201-116-C100   100 µg 194.00 USD Add To Cart
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Product Specification
MW: ~52kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. Recombinant human annexin V fused to GST.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Affinity purified.
FORMULATION: Lyophilized. Contains PBS, pH 7.4, and 0.02% sodium azide.
RECONSTITUTION: Reconstitute with 100µl sterile water.
SPECIFICITY: Binds to phosphatidylserine (PS).
BIOLOGICAL ACTIVITY: Exhibits anti-phospholipase activity.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Reconstituted solutions can be kept at 4°C under sterile conditions for one week or should be frozen in aliquots at -20°C. Repeated freeze/thaw cycles (>3) may result in an adverse effect on the activity of annexin V.
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-209-257 Revised 18-May-06
Annexin V (mouse) (recombinant) (Alexa Fluor® 488)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-209-257-T100   100 tests 245.00 USD Add To Cart
Product Specification
QUANTITY: 100 tests/500µl.
FORMULATION: Liquid. Contains 0.02% sodium azide.
SPECIFICITY: Binds phosphatidylserine (PS) residues.
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Protect from light. Do not freeze.
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Product Description
Alexa Fluor® 488-labelled recombinant mouse annexin shows bright green fluorescence (Ex(max): 495nm; Em(max): 519nm).
Product Specific Literature References
[1] Engagement of the antigen-receptor on immature murine B lymphocytes results in death by apoptosis: A. Norvell, et al.; J. Immunol. 154, 4404 (1995) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION General Protocol: Detection of apoptotic cells by flow cytometry

- Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS).
- Resuspend cells in 100µl binding buffer (10mM HEPES, pH 7.4, 140mM sodium chloride, 2.5mM calcium dichloride), add 5µl annexin V-Alexa Fluor® 488 to the cells and mix gently.
  For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1µg/ml or 1.5µM).
- Incubate for 15 min. at room temperature in the dark.
- Add 400µl binding buffer and analyze the staining in flow cytometry as soon as possible (within an hour).

Swiss-Prot link P48036: Annexin V (mouse)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-209-252 Revised 26-Aug-08
Annexin V (human) (recombinant) (APC)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-209-252-T020   20 tests 106.00 USD Add To Cart
ALX-209-252-T100   100 tests 279.00 USD Add To Cart
Product Specification
MW: ~35.8kDa.
SOURCE/HOST: Produced in E. coli.
QUANTITY: 20 tests/100µl; 100 tests/500µl.
PURITY: ≥98% (SDS-PAGE, HPLC)
FORMULATION: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Do not freeze. Protect from light.
Product Description
APC-labelled recombinant human annexin V is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable MAb (FITC-labelled or labelled secondary antibody). The fluorochrome APC (allophycocyanin) shows far red fluorescence (Ex(max): 650nm; Em(max): 660nm).
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:

 - Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2;
    filtered through 0.2µm pore filter);
 - Brief centrifugation before use is recommended
 - Wash cells in PBS by gentle shaking or pipetting up and down
 - resuspend cells in binding buffer; adjust cell density to 2-5x105/ml
 - Take 195µl cell suspension and add 5µl Annexin V-FITC
 - Mix and incubate 10 min. in the dark at room temperature
 - Wash cells 1x with PBS and resuspend in 190µl binding buffer
 - FACS analysis

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281 : Annexin V (mouse)
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-209-258 Revised 29-May-06
Annexin V (human) (recombinant) (ATTO 488)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-209-258-T100   100 tests 260.00 USD Add To Cart
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Product Specification
MW: ~52kDa (SDS-PAGE).
QUANTITY: 100 tests/0.5ml
PURITY: ≥90%
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry or immunofluorescence.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least one year after receipt when stored at +4°C.
HANDLING: Do not freeze. Protect from light.
Product Description
New ATTO-fluorescent dyes show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 488 shows bright green fluorescence (λabs (max): 501nm, λem (max): 523nm, εmax: 90'000).
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:

- Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm filter).
- Adjust cells to a density between 200’000 and 500’000 cells/ml.
- Take 195µl cell suspension and add 5µl Annexin-ATTO 488. Mix and incubate 10 min. in the dark.
- Wash cells 1x and resuspend in 190µl binding buffer.
- Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells.
- Analyze by FACS.
Note: These are general recommendations. Optimal conditions must be determined individually for each application:

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-209-259 Revised 11-Oct-06
Annexin V (human) (recombinant) (ATTO 647N)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-209-259-T100   100 tests 260.00 USD Add To Cart
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Product Specification
MW: ~52kDa (SDS-PAGE).
QUANTITY: 100 tests/0.5ml
PURITY: ≥90%
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry or immunofluorescence.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least one year after receipt when stored at +4°C.
HANDLING: Protect from light. Do not freeze.
Product Description
New ATTO-fluorescent dyes show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 647N shows red fluorescence (λabs (max): 645nm, λem (max): 669nm, εmax: 120’000).
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:

- Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm filter).
- Adjust cells to a density between 200’000 and 500’000 cells/ml.
- Take 195µl cell suspension and add 5µl Annexin-ATTO 647N. Mix and incubate 10 min. in the dark.
- Wash cells 1x and resuspend in 190µl binding buffer.
- Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells.
- Analyze by FACS.
Note: These are general recommendations. Optimal conditions must be determined individually for each application:

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-209-002 Revised 02-Aug-06
Annexin V (human) (recombinant) (Biotin)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-209-002-T020   20 tests 106.00 USD Add To Cart
ALX-209-002-T100   100 tests 352.00 USD Add To Cart
ALX-209-002-T300   300 tests 531.00 USD Add To Cart
Product Specification
MW: ~35.8kDa.
QUANTITY: 20 tests/100µl; 100 tests/500µl; 300 tests/1'500µl.
PURITY: ≥98% (SDS-PAGE, HPLC)
FORMULATION: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
[1] Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers: H.A. Andree, et al.; J. Biol. Chem. 265, 4923 (1990) Abstract; Full Text
[2] Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages: V.A. Fadok, et al.; J. Immunol. 148, 2207 (1992) Abstract
[3] Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis: G. Koopman, et al.; Blood 84, 1415 (1994) Abstract
[4] Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro: C.H. Homburg, et al.; Blood 85, 532 (1995) Abstract
[5] A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth. 184, 39 (1995) Abstract
[6] Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl: S. Martin, et al.; J. Exp. Med. 182, 1545 (1995) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[8] CD95 engagement releases calcium from intracellular stores of long term activated, apoptosis-prone gammadelta T cells: P. Rovere, et al.; J. Immunol. 156, 4631 (1996) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:
- Brief centrifugation before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down
- Resuspend cells in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered
through 0.2µm pore filter); adjust cell density to 2-5x105/ml
- Take 195µl cell suspension and add 5µl Annexin V-Biotin
- Mix and incubate 15 minutes at room temperature
- Wash cells 2x in binding buffer
- Resuspend in binding buffer
- Add Streptavidin-FITC
- Mix and incubate for 30 minutes in the dark at room temperature
- Wash cells 1x in binding buffer and resuspend in 190µl binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1mg/ml)
- FACS analysis

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281 : Annexin V (mouse)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • Biotinylated Reagents Other Products
 
 
ALX-209-250 Revised 08-Aug-08
Annexin V (human) (recombinant) (FITC)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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