ALEXIS Biochemicals: Thaxtomin A

You are here: Product Lines > Nitric Oxide Pathway > Nitric Oxide Synthases [NOS] / Related Products > NOS Other Products

ALX-430-026

Revised 12-Sep-06

Calmidazolium chloride
SYNONYMS	1-[bis(p-Chlorophenyl)methyl]-3-[2,4-dichloro-β-(2,4-dichlorobenzyloxy)phenethyl] imidazolium chloride
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calmodulin Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-430-026-M010		10 mg	85.00 USD
ALX-430-026-M050		50 mg	340.00 USD

Product Specification

FORMULA:	C₃₁H₂₃Cl₇N₂O
MW:	687.7
CAS NUMBER:	57265-65-3
PURITY:	≥98%
APPEARANCE:	White powder.
SOLUBILITY:	Soluble in methanol, 100% ethanol, chloroform, DMSO or propylene glycol; almost insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C

Product Description

Calmodulin antagonist. At least 150 times more potent than trifluoperazine (Prod. No. ALX- 550-310) as an inhibitor of brain calmodulin-dependent phosphodiesterase. Inhibitor of voltage-gated Ca²⁺ channels. Blocks Ca²⁺-calmodulin binding to NOS.

Product Specific Literature References

Inhibitors of calmodulin impair the constitutive but not the inducible nitric oxide synthase activity in the rat aorta: V.B. Schini & P.M. Vanhoutte; J. Pharmacol. Exp. Ther. 261, 553 (1992) Abstract
Calmidazolium, a calmodulin inhibitor, inhibits endothelium-dependent relaxations resistant to nitro-L-arginine in the canine coronary artery: S. Illiano, et al.; Br. J. Pharmacol. 107, 387 (1992) Abstract
The calmodulin antagonist calmidazolium stimulates release of nitric oxide in neuroblastoma N1E-115 cells: J. Hu & E.E. el-Fakahany; Neuroreport 4, 198 (1993) Abstract
Vasoactive intestinal peptide/pituitary adenylate cyclase-activating peptide-dependent activation of membrane-bound NO synthase in smooth muscle mediated by pertussis toxin-sensitive Gi1-2: K.S. Murthy & G.M. Makhlouf; J. Biol. Chem. 269, 15977 (1994) Abstract
Mitochondrial nitric oxide synthase is constitutively active and is functionally upregulated in hypoxia: Z. Lacza, et al.; Free Radic. Biol. Med. 31, 1609 (2001) Abstract
Mitochondrial calcium uptake stimulates nitric oxide production in mitochondria of bovine vascular endothelial cells: E.N. Dedkova, et al.; Am. J. Physiol. Cell Physiol. 286, C406 (2004) Abstract; Full Text
Neural tube closure depends on nitric oxide synthase activity: A. Nachmany, et al.; J. Neurochem. 96, 247 (2006) Abstract

Further Categories Containing This Product:

NOS Other Products • Ca2+ Channels (L-type) • Phosphodiesterases / Related Products

ALX-850-006

Revised 25-May-07

NOSdetect™ Assay Kit
PRODUCT LINE	Nitric Oxide Pathway
PRODUCT CATEGORY	Nitric Oxide & NOS Detection / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-006-KI01		1 Kit	390.00 USD

Product Specification

QUANTITY:	50 assays.
APPLICATION:	For the measurement of nitric oxide synthase (NOS) activity.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C

Product Description

Measuring NOS activity by monitoring the conversion of L-arginine to L-citrulline is currently the standard assay for NOS activity in both crude and purified enzyme preparations. Advantages of the NOSdetect™ Assay Kit (Stratagene) include the use of radioactive substrates ([³H]-arginine or [¹⁴C]arginine) that enable sensitivity to the picomole level, as well as the specificity of the assay for the NOS pathway due to the direct enzymatic conversion of L-arginine to L-citrulline in eukaryotic cells. Additionally, the easy separation of neutrally charged L-citrulline from positively charged L-arginine allows hundreds of assays to be performed in less than an hour.

General Information

MANUFACTURER

Manufactured by Stratagene.

Further Categories Containing This Product:

Quantification Kits • NOS Other Products

ALX-630-109

Revised 14-Oct-08

Thaxtomin A
SYNONYMS	Thaztomin A
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Toxins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-630-109-M001		1 mg	90.00 USD
ALX-630-109-M005		5 mg	360.00 USD

Product Specification

FORMULA:	C₂₂H₂₂N₄O₆
MW:	438.4
CAS NUMBER:	122380-18-1
SOURCE/HOST:	Isolated from Streptomyces sp.
PURITY:	≥96% (HPLC)
APPEARANCE:	Orange solid.
SOLUBILITY:	Soluble in acetone, 100% ethanol or methanol.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions are stable for at least 3 months when stored at -20°C.
HAZARD:	HARMFUL.

Product Description

Phytotoxin. Causes plant cell necrosis at nanomolar concentrations. Demonstrated to be produced by bacterial nitric oxide synthases (NOS).

Product Specific Literature References

Selection and characterization of microorganisms utilizing thaxtomin A, a phytotoxin produced by streptomyces scabies: C.L. Doumbou, et al.; Appl. Environ. Microbiol. 64, 4313 (1998) Abstract; Full Text
Involvement of a cytochrome P450 monooxygenase in thaxtomin A biosynthesis by Streptomyces acidiscabies: F.G. Healy, et al.; J. Bacteriol. 184, 2019 (2002) Abstract; Full Text
An Arabidopsis mutant resistant to thaxtomin A, a cellulose synthesis inhibitor from Streptomyces species: W.R. Scheible, et al.; Plant Cell 15, 1781 (2003) Abstract; Full Text
More chemistry of the thaxtomin phytotoxins: R.R. King, et al.; Phytochemistry 64, 1091 (2003) Abstract
Nitration of a peptide phytotoxin by bacterial nitric oxide synthase: J.A. Kers, et al.; Nature 429, 79 (2004) Abstract
Thaxtomin A induces programmed cell death in Arabidopsis thaliana suspension-cultured cells: I. Duval, et al.; Planta 222, 820 (2005) Abstract

Further Categories Containing This Product:

Apoptosis Inducers & Inhibitors Other Products • NOS Other Products • Non-apoptotic Cell Death / Necrosis

Online Product Ordering

Clipboard [0]

Your items will be kept only for the duration of your visit.