ALEXIS Biochemicals: UbcH9 (recombinant)

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ALX-201-150

Revised 05-Jul-06

Peptidyl-prolyl cis-trans Isomerase CPR6 (yeast) (recombinant)
SYNONYMS	CPR6 (yeast) (recombinant) PPIase Cpr6 (yeast) (recombinant)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Immunophilins / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-150-C025		25 µg	290.00 USD

Product Specification

EC:	5.2.1.8
SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	23mg/ml
PURITY:	≥99%
FORMULATION:	Liquid. In 40mM HEPES/KOH, pH 7.5, containing 20mM potassium chloride.
APPLICATION:	Western Blot
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-80°C
HANDLING:	For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.

Product Specific Literature References

Cpr6 and Cpr7, two closely related Hsp90-associated immunophilins from Saccharomyces cerevisiae, differ in their functional properties: C. Mayr, et al.; J. Biol. Chem. 275, 34140 (2000) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P53691: Peptidyl-prolyl cis-trans isomerase CPR6 (Saccharomyces cerevisiae (Baker's yeast))

General Literature References

Heat-shock protein 90, a chaperone for folding and regulation: D. Picard; Cell. Mol. Life Sci. 59, 1640 (2002), (Review) Abstract

Further Categories Containing This Product:

Enzymes • Recombinant Proteins / Fusion Proteins

ALX-201-119

Revised 01-Mar-05

Phosphoinositide 3-kinase p110α/p85 (bovine) (recombinant)
SYNONYMS	PI(3)K p110α/p85 (bovine) (recombinant)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Phosphoinositide 3-kinase [PI(3)K] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-119-C005		5 µg	440.00 USD

Product Specification

MW:	p110α: ~124.3kDa; p85: ~83.5kDa.
SOURCE/HOST:	Produced in Sf9 cells. Bovine PI(3)K p110α/p85 dimer untagged.
CONCENTRATION:	0.1mg/ml
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. Recombinant protein in 25mM HEPES, pH 8.0, 25mM sodium chloride, 2.5mM magnesium dichloride and 50% glycerol.
SPECIFIC ACTIVITY:	~1nmol/mg/min using phosphatidyl-inositol as the substrate.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

Phosphoinositide 3- kinases (PI(3)Ks) phosphorylate the 3'-OH position of inositol phospholipids. The heterodimeric complex consists of a catalytic subunit (p110α) and a regulatory adaptor subunit (p85). The PI(3)K regulates the MAP kinase and the Akt/PKB signalling pathway that is involved in pivotal cellular processes such as mitogenesis, apoptosis and cytoskeletal functions, and also plays an important role in the development of human cancer.

Further Categories Containing This Product:

Enzymes • Recombinant Proteins / Fusion Proteins

ALX-201-055

Revised 17-Apr-07

Phosphoinositide 3-kinase p110γ (human) (recombinant)
SYNONYMS	PI(3)K p110γ (human) (recombinant)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Phosphoinositide 3-kinase [PI(3)K] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-055-C010		10 µg	330.00 USD

Product Specification

EC:	2.7.1.137
SOURCE/HOST:	Produced in Sf9 cells. Full length PI(3)K p110γ (human phosphoinositide-3-kinase p110γ) is fused at the N-terminus to a His-tag.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 10mM HEPES, pH 7.5, containing 100mM sodium chloride, 0.5mM magnesium chloride and 50% glycerol.
SPECIFIC ACTIVITY:	~4.5nmol/mg/min using phosphatidylinositol as the substrate (lipid kinase activity test).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-20°C

Product Specific Literature References

Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase: B. Stoyanov, et al.; Science 269, 690 (1995) Abstract
Linkage of G protein-coupled receptors to the MAPK signaling pathway through PI 3-kinase gamma: M. Lopez-Ilasaca, et al.; Science 275, 394 (1997) Abstract
Lipid kinase and protein kinase activities of G-protein-coupled phosphoinositide 3-kinase gamma: structure-activity analysis and interactions with wortmannin: S. Stoyanova, et al.; Biochem. J. 324, 489 (1997) Abstract; Full Text
Gbetagamma stimulates phosphoinositide 3-kinase-gamma by direct interaction with two domains of the catalytic p110 subuni: D. Leopoldt, et al.; J. Biol. Chem. 273, 7024 (1998) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P48736: PI(3)K p110γ (human)

Lipid kinase activity test:
Kinase reaction buffer: 20mM TRIS-HCl, pH 7.4; 4mM MgCl₂; 100mM sodium chloride.
- Reaction mixture in total volume of 100ml was:
1x reaction buffer (final concentration), enzyme (4 reactions with 1, 2, 3 or 4µg were performed), 10µg phosphatidylinositol, 20µM cold ATP and 10µCi ³²P-ATP.
- Reaction was started by addition of the ³²P-ATP and incubate for 10 minutes at 37°C.
- Reaction was stopped by adding 150µl 1M HCl.
- Lipids were extracted with 400µl chloroform:methanol (1:1) by vortexing.
- Extracts were washed three times with 200µl 1M HCl.
- Incorporation of ³²P in the extracted lipids was measured by mixing extract with 3ml scintillation cocktail (ROTH) and counting in a liquid scintillation analyzer.
- To determine specific activity of PI3K p110γ the assay was performed under the same conditions stated above by using 10µCi ³²P-ATP with a value of 10'000 units.
- Average value of measurements of the lipid extracts of the performed 4 reactions was 227 units for 1µg. Dividing 10’000 by 227 a factor of 44 times less for the incorporation was determined.
-Total ATP (20µM) was divided by 44 giving 0.454µM for 10 minutes reaction time or 45.4M/min/µg. Recalculation of this value for a 1 liter solution results in 4.5 nmoles/min/mg.

Further Categories Containing This Product:

Enzymes • Recombinant Proteins / Fusion Proteins

ALX-201-221

Revised 02-Oct-06

Pin1 (human) (recombinant)
SYNONYMS	Peptidyl-prolyl cis-trans Isomerase Pin1 (human) (recombinant) PPIase Pin1 (human) (recombinant) Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 (human) (recombinant) PPIase NIMA-interacting 1 (human) (recombinant) Rotamase Pin1 (human) (recombinant)
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Alzheimer Research Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-221-C100		100 µg	325.00 USD

Product Specification

MW:	~18.2kDa
SOURCE/HOST:	Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1 produced in E. coli.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 20mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 5mM DTT and 20% glycerol.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Further Categories Containing This Product:

Stress & Heat Shock Proteins Other Products • Antiviral Signalling / Related Products • Recombinant Proteins / Fusion Proteins • p53 Other Products

ALX-201-282

Revised 18-Mar-08

PRMT1 (human) (recombinant) (His)
SYNONYMS	Protein Arginine N-Methyltransferase 1 (human) (recombinant) (His)
PRODUCT LINE	Nitric Oxide Pathway
PRODUCT CATEGORY	PRMTs, Mono- & Dimethylarginine / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-282-C002		2 µg	280.00 USD

Product Specification

EC:	EC 2.1.1.-
SOURCE/HOST:	Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION:	0.5mg/ml
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY:	~50-100ng are required for standard HAT assay.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link Q99873: PRMT-1 (human)

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-522-093

Revised 27-Mar-08

Profilin (Toxoplasma gondii) (recombinant)
SYNONYMS	Inflammatory Profilin (Toxoplasma gondii) (recombinant) Toll-like Receptor 11 Ligand (Toxoplasma gondii) (recombinant) TLR11 Ligand (Toxoplasma gondii) (recombinant)
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	Actin / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-093-C010		10 µg	230.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse
SEQUENCE:	aa (1-163)
MW:	~47kDa
SOURCE/HOST:	Produced in E. coli. Full length profilin (aa 1-163) from Toxoplasma gondii is fused to a N-terminal tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile, endotoxin-free water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to mouse TLR11.
BIOLOGICAL ACTIVITY:	Activates and induces TLR11-dependent IL-12p40 production from dendritic cells.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

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General Information

The initial recognition of pathogens during host defense is mediated by a family of receptors termed toll-like receptors (TLR). TLRs recognize conserved microbial components, termed pathogen-associated molecular patterns (PAMPs). To date, 11 TLRs have been cloned in mammals and each receptor appears to be involved in the recognition of a unique set of PAMPs that are distinct in their chemical nature and structure. TLR11 has a role in uropathogenic E. coli infection and senses the profilin protein from the protozoan parasite Toxoplasma gondii. Profilins are a class of small actin-binding proteins present only in eukaryotic cells. T. gondii profilin shares significant homology only with profilin genes from other apicomplexan protozoa, such as the malaria parasite, Plasmodium falciparum, suggesting that TLR11 may be involved in the recognition of these parasites.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q58NA1: Profilin (Toxoplasma gondii)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

A toll-like receptor that prevents infection by uropathogenic bacteria: D. Zhang, et al.; Science 303, 1522 (2004) Abstract
TLR11 activation of dendritic cells by a protozoan profilin-like protein: F. Yarovinsky, et al.; Science 308, 1626 (2005) Abstract
Of mice and man: TLR11 (finally) finds profilin: F.N. Lauw, et al.; Trends Immunol. 26, 509 (2005) Abstract
Toll-like receptor recognition regulates immunodominance in an antimicrobial CD4+ T cell response: F. Yarovinsky, et al.; Immunity 25, 655 (2006) Abstract

ALX-505-007	PBS (endotoxin-free)
ALX-505-008	ddWater (endotoxin-free)

Progranulin (PGRN) has been shown to be a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signalling cascades that control cell cycle progression and cell motility. PGRN has also been linked to tumorigenesis. The function of PGRN in the central nervous system (CNS) is also of interest, because mutations in the PGRN gene have been identified in cases of frontotemporal degeneration (FTD).
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P28799: Granulins (human) [Precursor] FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER	Manufactured by AdipoGen, Inc.

ALX-804-737	Monoclonal Antibody to Progranulin (human) (PG359-7)
AG-P0731	Progranulin (human) ELISA Kit
AG-PM0731	Progranulin (mouse) ELISA Kit
ALX-201-389	Progranulin (mouse) (recombinant)

Progranulin (PGRN) has been shown to be a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signalling cascades that control cell cycle progression and cell motility. PGRN has also been linked to tumorigenesis. The function of PGRN in the central nervous system (CNS) is also of interest, because mutations in the PGRN gene have been identified in cases of frontotemporal degeneration (FTD).
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P28798: Granulins (mouse) [Precursor] FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER	Manufactured by AdipoGen, Inc.

ALX-201-377	Progranulin (human) (recombinant)
ALX-804-737	Monoclonal Antibody to Progranulin (human) (PG359-7)
AG-PM0731	Progranulin (mouse) ELISA Kit
AG-P0731	Progranulin (human) ELISA Kit

SOURCE/HOST:	Produced in HEK 293 cells. Signal peptide and mature human progranulin (aa 1-593) is fused at the C-terminus to a FLAG^®-tag.
CONCENTRATION:	1mg/ml
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg protein (LAL-test).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

MW:	87kDa (homotrimer of 29kDa)
SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 25mM TRIS-HCl, pH 7.5, containing 50% glycerol, 50mM sodium chloride, 1mM DTT and 1mM EDTA.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-20°C
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stable for at least 4 weeks when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.