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Viral Signalling
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AG-AE0631 Revised 11-Jan-08
ACE2 (human) ELISA Kit
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SYNONYMS Angiotensin-converting Enzyme 2 (human) ELISA Kit
SARS Receptor (human) ELISA Kit
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin Converting Enzyme [ACE] & ACE2 / Related Products
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AG-AE0631EK-KI01   96 wells 590.00 USD Add To Cart
AG-AE0631TP-KI01 Twin Plex 2x96 wells 890.00 USD Add To Cart
AG-AE0631PP-KI01 Penta Plex 5x96 wells 1'870.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 293pg/ml (range 0 to 50ng/ml)
APPLICATION: For the quantitative determination of ACE2 in human urine samples or cell culture supernatants.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
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MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
SARS Coronavirus / Related Products • ELISA & EIA Kits
 
 
ALX-201-221 Revised 02-Oct-06
Pin1 (human) (recombinant)
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SYNONYMS Peptidyl-prolyl cis-trans Isomerase Pin1 (human) (recombinant)
PPIase Pin1 (human) (recombinant)
Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 (human) (recombinant)
PPIase NIMA-interacting 1 (human) (recombinant)
Rotamase Pin1 (human) (recombinant)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Alzheimer Research Other Products
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ALX-201-221-C100   100 µg 325.00 USD Add To Cart
Product Specification
MW: ~18.2kDa
SOURCE/HOST: Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1 produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 20mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 5mM DTT and 20% glycerol.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Stress & Heat Shock Proteins Other Products • Antiviral Signalling / Related Products • Recombinant Proteins / Fusion Proteins • p53 Other Products
 
 
ALX-201-247 Revised 18-Mar-08
NS3-NS4A (HCV) (recombinant) (His)
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SYNONYMS Nonstructural Protein 3-NS4A (HCV) (recombinant) (His)
Hepacivirin-NS4A (HCV) (recombinant) (His)
PRODUCT LINE Viral Signalling
PRODUCT CATEGORY Hepatitis Viruses / Related Products
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ALX-201-247-C100   100 µg 210.00 USD Add To Cart
Product Specification
MW: ~68.7kDa (NS3 subunit); ~6.2kDa (NS4A subunit)
EC: 3.4.21.98
SOURCE/HOST: Produced in E. coli. Recombinant hepatitis C virus (HCV) NS3-NS4A serine protease complex is fused to a His-tag.
CONCENTRATION: 379ng/μl (5µM)
PURITY: ≥85% (SDS-PAGE)
FORMULATION: Liquid. In 25mM HEPES, pH 7.6, containing 1mM EDTA, 3mM DTT, 0.5M sodium chloride, 0.2% Triton X-100 and 20% glycerol.
BIOLOGICAL ACTIVITY: IC50 (lot-specific)
APPLICATION: Suitable for screenings.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-330 Revised 24-Jul-06
ACE2 (human):Fc (human) (recombinant)
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SYNONYMS Angiotensin-converting Enzyme 2 (human):Fc (human) (recombinant)
SARS Receptor (human):Fc (human) (recombinant)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin Converting Enzyme [ACE] & ACE2 / Related Products
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ALX-201-330-C050   50 µg 370.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. The signal peptide sequence and the extracellular domain of human ACE2 (angiotensin-converting enzyme 2) (aa 1-740) are fused at the C-terminus to the Fc portion of human IgG1.
CONCENTRATION: 1mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL test).
BIOLOGICAL ACTIVITY: Converts angiotensin I to angiotensin-(1-9).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link Q9NRA7: Angiotensin converting enzyme-like protein (human)
MANUFACTURER Manufactured by Adipogen, Inc.
Further Categories Containing This Product:
SARS Coronavirus / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-356 Revised 18-Mar-08
ACE2, Soluble (human) (recombinant)
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SYNONYMS Angiotensin-converting Enzyme 2, Soluble (human) (recombinant)
SARS Receptor, Soluble (human) (recombinant)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin Converting Enzyme [ACE] & ACE2 / Related Products
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ALX-201-356-C010   10 µg 295.00 USD Add To Cart
ALX-201-356-C050   50 µg 795.00 USD Add To Cart
Product Specification
EC: 3.4.17.-
SOURCE/HOST: Produced in HEK 293 cells. The signal peptide sequence and the extracellular domain of human ACE2 (angiotensin-converting enzyme 2) (aa 1-740) are fused at the C-terminus to a FLAG®-tag.
CONCENTRATION: 1mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL-test).
BIOLOGICAL ACTIVITY: Converts angiotensin I to angiotensin-(1-9).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9NRA7: Angiotensin converting enzyme-like protein (human)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
SARS Coronavirus / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-210-028 Revised 01-Mar-07
Polyclonal Antibody to TRAF6 (Bur 30)
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SYNONYMS anti-Tumor Necrosis Factor (TNF) Receptor-associated Factor 6 PAb (Bur 30)
anti-RING Finger Protein 85 PAb (Bur 30)
anti-Interleukin-1 Signal Transducer PAb (Bur 30)
PRODUCT LINE Inflammation
PRODUCT CATEGORY TRAFs / Related Products
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ALX-210-028-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 451-469 (D451AKPELLAFQRPTIPRNPK469) of human TRAF6 (tumor necrosis factor (TNF) receptor-associated factor 6). This sequence is completely conserved in mouse TRAF6.
SPECIFICITY: Recognizes human and mouse TRAF6. Detects a band of ~65kDa by Western blot. Does not cross-react with other members of the TRAF family.
APPLICATION: Immunohistochemistry (paraffin sections (1:1'000))
Western Blot (1:500-1:2'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -80°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies: J.M. Zapata, et al.; J. Immunol. 165, 5084 (2000) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Autoimmunity & Self Tolerance Other Products • Antiviral Signalling / Related Products • Polyclonal Antibodies
 
 
ALX-210-352 Revised 30-Oct-06
Polyclonal Antibody to MDA5 (mouse) (AL180)
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SYNONYMS anti-Melanoma Differentiation-associated Gene 5 (mouse) PAb (AL180)
anti-Helicard (mouse) PAb (AL180)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
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ALX-210-352-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Contains 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse MDA5 (melanoma differentiation-associated gene 5) (aa 2-208).
SPECIFICITY: Recognizes mouse MDA5.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Overexpression of Helicard, a CARD-Containing Helicase Cleaved during Apoptosis, Accelerates DNA Degradation: M. Kovacsovics, et al.; Curr. Biol. 12, 838 (2002) Abstract
General Information
MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract
Related Products
Further Categories Containing This Product:
NF-kB Pathway Other Products • Antiviral Signalling / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • Polyclonal Antibodies
 
 
ALX-210-367 Revised 25-Sep-08
Polyclonal Antibody to Toll-like Receptor 3 (mouse)
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SYNONYMS anti-TLR3 (mouse) PAb
anti-CD283 (mouse) PAb
PRODUCT LINE Inflammation
PRODUCT CATEGORY TLR Antibodies
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ALX-210-367-R200   200 µl 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptides corresponding to aa 135-150, 828-844 and 876-891 of mouse TLR3 (Toll-like receptor 3).
SPECIFICITY: Recognizes mouse TLR3. Does not cross-react with human TLR3.
APPLICATION: Flow Cytometry (cell surface & intracellular (1μg/106 cells) (see [2]))
Immunohistochemistry (paraffin sections (1:50), frozen sections (see [1]))
Western Blot (1-3µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
[1] Viral double-stranded RNA aggravates lupus nephritis through Toll-like receptor 3 on glomerular mesangial cells and antigen-presenting cells: P.S. Patole, et al.; J. Am. Soc. Nephrol. 16, 1326 (2005) Abstract
[2] Toll-like receptor-7 modulates immune complex glomerulonephritis: R.D. Pawar, et al.; J. Am. Soc. Nephrol. 17, 141 (2006) Abstract
[3] Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice: P.S. Patole, et al.; Nephrol. Dial. Transplant. 21, 3062 (2006) Abstract
Further Categories Containing This Product:
Antiviral Signalling / Related Products • Polyclonal Antibodies • CD Antibodies
 
 
ALX-210-444 Revised 25-Jul-07
Polyclonal Antibody to ACE2 (human)
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SYNONYMS anti-Angiotensin-converting Enzyme 2 (human) PAb
anti-SARS Receptor (human) PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin Converting Enzyme [ACE] & ACE2 / Related Products
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ALX-210-444-C100   100 µg 340.00 USD Add To Cart
ALX-210-444B-C050 Biotin 50 µg 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Purified IgG.
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human ACE2 (angiotensin-converting enzyme 2).
SPECIFICITY: Recognizes human ACE2.
APPLICATION: ELISA (direct and indirect: 1:2’000-1:10’000)
Western Blot 1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
SARS Coronavirus / Related Products • Polyclonal Antibodies
 
 
ALX-210-908 Revised 14-Jan-08
Polyclonal Antibody to TRIF (human) (AL227)
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SYNONYMS anti-TICAM-1 (human) PAb (AL227)
anti-TIR-containing Adaptor Molecule-1 (human) PAb (AL227)
anti-Toll-interleukin-1 Receptor [TIR] Domain-containing Adaptor Inducing Interferon-β [IFN-β] (human) PAb (AL227)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Toll-IL-1 Receptor Adaptors / Related Products
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ALX-210-908-C050   50 µg 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 4-31 (T4GPSLPSAFDIL
GAAGQDKLLYLKHKLK31) of N-terminal human TRIF [TICAM-1].
SPECIFICITY: Recognizes human TRIF.
APPLICATION: Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
The human adaptor SARM negatively regulates adaptor protein TRIF-dependent Toll-like receptor signaling: M. Carty, et al.; Nat. Immunol. 7, 1074 (2006) Abstract
Interferon regulatory factor 3-dependent responses to lipopolysaccharide are selectively blunted in cord blood cells: E. Aksoy, et al.; Blood 109, 2887 (2007) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Antiviral Signalling / Related Products • Polyclonal Antibodies
 
 
ALX-210-929 Revised 16-Apr-08
Polyclonal Antibody to Cardif (human) (AT107)
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SYNONYMS anti-CARD Adapter Inducing Interferon-β (human) PAb (AT107)
anti-IPS-1 (human) PAb (AT107)
anti-Interferon-β Promoter Stimulator Protein 1 (human) PAb (AT107)
anti-MAVS (human) PAb (AT107)
anti-Mitochondrial Antiviral Signalling Protein (human) PAb (AT107)
anti-VISA (human) PAb (AT107)
anti-Virus-induced Signalling Adapter (human) PAb (AT107)
PRODUCT LINE Immunology
PRODUCT CATEGORY Antiviral Signalling / Related Products
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ALX-210-929-C100   100 µg 370.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).
SPECIFICITY: Recognizes human cardif.
APPLICATION: Immunocytochemistry (1:500)
Immunoprecipitation (1:100)
Western Blot (1:2’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
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Product Specific Literature References
Regulation of antiviral responses by a direct and specific interaction between TRAF3 and Cardif : S. K. Saha; EMBO J. 25, 3257 (2006) Abstract
Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008) Abstract
General Information
RIG-I (retinoic acid-inducible gene I; Ddx58) and Mda5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons.
Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and Mda5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005) Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005) Abstract
CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006) Abstract
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • NF-kB Pathway Other Products • Polyclonal Antibodies
 
 
ALX-210-932 Revised 28-Feb-07
Polyclonal Antibody to RIG-I (human) (AT111)