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Golgi Apparatus
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Items 13 of 13
ALX-210-127 Revised 09-Aug-07
Polyclonal Antibody to β-COP
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SYNONYMS anti-β-Coat Protein PAb
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Golgi Apparatus
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ALX-210-127-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
PURITY DETAIL: Purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 496-513 (E496AGELKPEEEITVGPVQK513) of rat β-COP (β-coat protein).
SPECIFICITY: Recognizes human, mouse and rat β-COP. Detects a band of~110kDa by Western blot.
APPLICATION: Immunocytochemistry (1:2’000) [1]
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
[1] Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin: R. Duden, et al.; Cell 64, 649 (1991) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-790 Revised 29-Sep-05
Polyclonal Antibody to SCAMP1
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SYNONYMS anti-Secretory Carrier Membrane Protein 1 PAb
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Endocytosis
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ALX-210-790-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-17 (M1SDFDSNPFADPDLNNP17C) of human SCAMP1 (secretory carrier membrane protein 1). This sequence is completely conserved in rat and pig SCAMP1.
SPECIFICITY: Recognizes human, mouse and rat SCAMP 1. Detects a band of ~31kDa by Western blot.
APPLICATION: Immunoprecipitation
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-167-026.
Related Products
Further Categories Containing This Product:
Synaptic Proteins / Related Products • Golgi Apparatus • Polyclonal Antibodies
 
 
ALX-210-857 Revised 01-Dec-04
Polyclonal Antibody to SCAMP2
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SYNONYMS anti-Secretory Carrier Membrane Protein 2 PAb
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Endocytosis
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ALX-210-857-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Others
SOURCE/HOST: From rabbit.
FORMULATION: Purified. Contains 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-17 (M1SAFDTNPFADPVDVNP17) of human SCAMP2 (secretory carrier membrane protein 2).
SPECIFICITY: Recognizes mouse and hamster SCAMP 2.
APPLICATION: Western Blot.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-167-027.
Further Categories Containing This Product:
Synaptic Proteins / Related Products • Golgi Apparatus • Polyclonal Antibodies
 
 
ALX-210-884 Revised 03-Jun-05
Polyclonal Antibody to α-COP
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SYNONYMS anti-α-Coat Protein PAb
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Golgi Apparatus
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ALX-210-884-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-19 (M1LTKFETKSARVK
GLSFHP19) of rat α-COP (coat-protein (α subunit)).
SPECIFICITY: Recognizes human and rat α-COP. Detects bands of ~140kDa (representing α-COP) and ~100kDa (unidentified) by Western blot.
APPLICATION: Immunocytochemistry: (3µg/ml)
Immunocytochemical staining results in α-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm.
Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-153-057.
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Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-886 Revised 07-Dec-06
Polyclonal Antibody to β'-COP (rat)
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SYNONYMS anti-β'-Coat Protein (rat) PAb
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Golgi Apparatus
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ALX-210-886-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 494-511 (K494VLAAQETHEGV
TEDGIE511) of rat β’-COP (coat-protein (β’ subunit)).
SPECIFICITY: Recognizes rat β’-COP. Detects a band of ~115kDa by Western blot.
APPLICATION: Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-153-058.
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Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-887/1 Revised 14-Aug-07
Polyclonal Antibody to COPII (rat)
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SYNONYMS anti-Coat Protein II (rat) PAb
anti-SEC23 (rat) PAb
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Golgi Apparatus
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ALX-210-887/1-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-18 (M1TTYLEFIQQNEER
DGVR18) of rat COPII (coat protein II; SEC23).
SPECIFICITY: Recognizes mouse and rat COPII. Detects a band of ~85kDa by Western blot.
APPLICATION: Immunocytochemistry
Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-153-059.
Related Products
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-350-019 Revised 16-Oct-08
(+)-Brefeldin A
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SYNONYMS BFA
Ascotoxin
Decumbin
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics - Apoptosis Inducers & Inhibitors
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ALX-350-019-M005   5 mg 80.00 USD Add To Cart
ALX-350-019-M010   10 mg 130.00 USD Add To Cart
ALX-350-019-M025   25 mg 270.00 USD Add To Cart
ALX-350-019-M050   50 mg 420.00 USD Add To Cart
Product Specification
FORMULA: C16H24O4
MW: 280.4
CAS NUMBER: 20350-15-6
MERCK INDEX: 14: 1369
SOURCE/HOST: Isolated from Eupenicillium brefeldianum.
PURITY: ≥97% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, methanol, acetone or ethyl acetate.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: HARMFUL.

Product Description
Inhibitor of intracellular protein transport and protein secretion, interfering with trafficking in the trans-Golgi network, leading to the accumulation of cycling proteins in ERGIC clusters. Blocks ADP-ribosylation factor (Arf) in an inactive GDP-bound conformation and thereby prevents binding of COPI coats to ERGIC and Golgi membranes. Upon brefeldin A treatment the Golgi rapidly tubulates and fuses with the ER by an energy-, temperature-, and microtubule-dependent process. In contrast, the drug has little effect on the ERGIC, which keeps its identity, although the ERGIC clusters become larger and more uniformly distributed in the cytoplasm of the cells. Inhibits intracellular collagen degradation. Also inhibits apical Na+ channels in epithelia. Induces apoptosis.
Product Specific Literature References
Decumbin, a new compound from a species of Penicillium: V.L. Singleton, et al.; Nature 181, 1072 (1958)
Über die Isolierung neuer Stoffwechselprodukte aus Penicillium brefeldianum Dodge.: E. Harri, et al.; Helv. Chim. Acta 46, 1235 (1963)
Novel blockade by brefeldin A of intracellular transport of secretory proteins in cultured rat hepatocytes: Y. Misumi, et al.; J. Biol. Chem. 261, 11398 (1986) Abstract; Full Text
Brefeldin A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum: T. Fujiwara, et al.; J. Biol. Chem. 263, 18545 (1988) Abstract; Full Text
Brefeldin A, thapsigargin, and AIF4- stimulate the accumulation of GRP78 mRNA in a cycloheximide dependent manner, whilst induction by hypoxia is independent of protein synthesis: B.D. Price, et al.; J. Cell. Physiol. 152, 545 (1992) Abstract
Brefeldin A and the endocytic pathway. Possible implications for membrane traffic and sorting: W. Hunziker, et al.; FEBS Lett. 307, 93 (1992), (Review) Abstract
Brefeldin A inhibits degradation as well as production and secretion of collagen in human lung fibroblasts: C.R. Ripley, et al.; J. Biol. Chem. 268, 3677 (1993) Abstract
Brefeldin A is a potent inducer of apoptosis in human cancer cells independently of p53: R.G. Shao, et al.; Exp. Cell Res. 227, 190 (1996) Abstract
Brefeldin A inhibition of apical Na+ channels in epithelia: R.S. Fisher, et al.; Am. J. Physiol. 270, C138 (1996) Abstract
Brefeldin A: deciphering an enigmatic inhibitor of secretion: A. Nebenfuhr, et al.; Plant Physiol. 130, 1102 (2002), Review Abstract
NKT cells provide help for dendritic cell-dependent priming of MHC class I-restricted CD8+ T cells in vivo: D. Stober, et al.; J. Immunol. 170, 2540 (2003) Abstract; Full Text
Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70: K.F. Xu, et al.; PNAS 102, 2784 (2005) Abstract
Further Categories Containing This Product:
ADP Ribosylation Factor [ARF] / Related Products • Golgi Apparatus • Collagen / Related Products • Na+ Channels • Antitumor Antibiotics • Antibiotics - Antiviral / Anti-HIV • Antibiotics - Antifungal • Autophagy Other Products • Antitumor Agents (Apoptosis Inducers)
 
 
ALX-350-086 Revised 08-Apr-08
Nordihydroguaiaretic acid
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SYNONYMS NDGA
4,4'-(2,3-Dimethyl-1,4-butanediyl)bis[1,2-benzenediol]
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Other Natural Products for Inflammation Research
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ALX-350-086-G001   1 g 62.00 USD Add To Cart
Product Specification
FORMULA: C18H22O4
MW: 302.4
CAS NUMBER: 500-38-9
MERCK INDEX: 14: 6693
SOURCE/HOST: Synthetic.
PURITY: ≥95%
APPEARANCE: Crystalline powder.
SOLUBILITY: Soluble in 100% ethanol, methanol or acetone; slightly soluble in hot water.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C

Product Description
Antioxidant and antineoplastic. Lipoxygenase inhibitor. Stimulates the rapid retrograde movement of both Golgi stacks and the trans-Golgi network to the endoplasmatic reticulum (ER). Displays pleiotropic effects on cells, which include apoptosis, cell proliferation, differentiation and chemotaxis. Anticancer agent.
Product Specific Literature References
Inhibition of tumoral cell respiration and growth by nordihydroguaiaretic acid: M. Pavani, et al.; Biochem. Pharmacol. 48, 1935 (1994) Abstract
Nordihydroguaiaretic acid blocks protein transport in the secretory pathway causing redistribution of Golgi proteins into the endoplasmic reticulum: T. Fujiwara, et al.; J. Biol. Chem. 273, 3068 (1998) Abstract; Full Text
Effect of nordihydroguaiaretic acid on the secretion of lipoprotein lipase: S.M. Kim, et al.; J. Biochem. Mol. Biol. 35, 518 (2002) Abstract; Full Text
Mechanisms underlying the activation of large conductance Ca2+-activated K+ channels by nordihydroguaiaretic acid: H. Yamamura, et al.; Jpn. J. Pharmacol. 89, 53 (2002) Abstract
Direct interaction of the Golgi membrane with the endoplasmic reticulum membrane caused by nordihydroguaiaretic acid: T. Fujiwara, et al.; BBRC 301, 927 (2003) Abstract
Stepwise dissection of the intracellular fate of cationic cell-penetrating peptides: R. Fischer, et al.; J. Biol. Chem. 279, 12625 (2004) Abstract; Full Text
The anti-apoptotic effects of nordihydroguaiaretic acid: inhibition of cPLA(2) activation during TNF-induced apoptosis arises from inhibition of calcium signaling: C.A. Culver, et al.; Life Sci. 77, 2457 (2005) Abstract
Nordihydroguaiaretic acid is a potent in vitro scavenger of peroxynitrite, singlet oxygen, hydroxyl radical, superoxide anion and hypochlorous acid and prevents in vivo ozone-induced tyrosine nitration in lungs: E. Floriano-Sanchez, et al.; Free Radic. Res. 40, 523 (2006) Abstract
Nordihydroguaiaretic acid affects multiple dynein-dynactin functions in interphase and mitotic cells: K. Arasaki, et al.; Mol. Pharmacol. 71, 454 (2007) Abstract
Protective role of nordihydroguaiaretic acid (NDGA) against the genotoxic damage induced by ethynodiol diacetate in human lymphocytes in vitro: Y.H. Siddique, et al.; J. Environ. Biol. 28, 279 (2007) Abstract
Further Categories Containing This Product:
Golgi Apparatus • Lipoxygenases / Related Products • Natural Products - Antioxidants • Antitumor Agents (Enzyme Inhibitors) • Natural Products - Antitumor Reagents
 
 
ALX-350-240 Revised 05-Dec-07
Ilimaquinone
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SYNONYMS 3-[(Decahydro-1β,2β,4αβ-trimethyl-5-methylene-1-naphthyl)methyl]-2-hydroxy-5-methoxybenzoquinone
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Other Signal Transduction Pathway Modulators
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ALX-350-240-C100   100 µg 140.00 USD Add To Cart
Product Specification
FORMULA: C22H30O4
MW: 358.5
CAS NUMBER: 71678-03-0
PURITY: ≥98%
APPEARANCE: Yellow powder.
SOLUBILITY: Soluble in DMSO or 100% ethanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Displays antimicrobial, anti-HIV and anti-inflammatory properties. Induces reversible breakdown of Golgi membranes. Inhibits the association of β-COP and ADP-ribosylation factor to Golgi membranes. Interacts with methylation enzymes. Shows anticancer activity.
Product Specific Literature References
R.T. Luibrand, et al.; Tetrahedron 35, 609 (1979)
Complete vesiculation of Golgi membranes and inhibition of protein transport by a novel sea sponge metabolite, ilimaquinone: P.A. Takizawa, et al.; Cell 73, 1079 (1993) Abstract
Interactions of (-)-ilimaquinone with methylation enzymes: implications for vesicular-mediated secretion: H.S. Radeke, et al.; Chem. Biol. 6, 639 (1999) Abstract
Ilimaquinone inhibits gap-junctional communication prior to Golgi fragmentation and block in protein transport: V. Cruciani, et al.; Exp. Cell Res. 287, 130 (2003) Abstract
Natural products with anti-HIV activity from marine organisms: L.A. Tziveleka, et al.; Curr. Top. Med. Chem. 3, 1512 (2003), Review Abstract
Interaction of the smooth endoplasmic reticulum and mitochondria: J.G. Goetz & I.R. Nabi; Biochem. Soc. Trans. 34, 370 (2006), Review Abstract
Ilimaquinone, a marine sponge metabolite, displays anticancer activity via GADD153-mediated pathway: P.H. Lu, et al.; Eur. J. Pharmacol. 556, 45 (2007) Abstract
Further Categories Containing This Product:
Natural Products - Anti-inflammatory Agents • Golgi Apparatus • ADP Ribosylation Factor [ARF] / Related Products • Natural Products - Chemopreventive Agents • Natural Products - Antiviral / anti-HIV Agents • PRMTs, Mono- & Dimethylarginine / Related Products • Marine Natural Products
 
 
ALX-804-095 Revised 09-Aug-07
Monoclonal Antibody to trans-Golgi Network 38 (rat) (2F7.1)
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SYNONYMS anti-TGN38 (rat) MAb (2F7.1)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Golgi Apparatus
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ALX-804-095-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Rat
CLONE: 2F7.1
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-17 (L1PSASKPNNTS
SENNPP17) of N-terminal rat TGN38 (trans-Golgi network 38).
SPECIFICITY: Recognizes the luminal domain of rat TGN38. Detects a band of ~38kDa by Western blot, representing deglycosylated TGN38. Under normal Western blot conditions detects a band of ~85-95kDa depending on cell type.
APPLICATION: Flow Cytometry
Immunocytochemistry (1:1'000)
Western Blot (1:500)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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