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Caspase Recruitment Domain [CARD] Proteins / Related Products
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ALX-840-602 Revised 24-Nov-05
Positive Control (Cell Lysate) for Antibodies to Asc (human)
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SYNONYMS Positive Control (Cell Lysate) for Antibodies to Pycard (human)
PRODUCT LINE Immunology
PRODUCT CATEGORY Positive Controls (Cell Lysates & Extracts)
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-840-602-R100   100 µl 70.00 USD Add To Cart
Product Specification
SOURCE/HOST: Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human Asc (aa 1-195) fused at the C-terminus to a FLAG®-tag.
QUANTITY: 100µl corresponding to 2x105 cells.
FORMULATION: Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION: Positive control in Western blot for PAb to Asc (human) (AL177) (Prod. No. ALX-210-905). Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • Inflammasome
 
 
ALX-840-603 Revised 08-Mar-07
Positive Control (Cell Lysate) for Antibodies to NALP1 (human)
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SYNONYMS Positive Control (Cell Lysate) for Antibodies to NACHT-, LRR- and PYD-containing Protein 1 (human)
Positive Control (Cell Lysate) for Antibodies to DEFCAP (human)
Positive Control (Cell Lysate) for Antibodies to NAC (human)
Positive Control (Cell Lysate) for Antibodies to CARD7 (human)
Positive Control (Cell Lysate) for Antibodies to Caspase Recruitment Domain-containing Protein 7 (human)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Inflammasome
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-840-603-R100   100 µl 70.00 USD Add To Cart
Product Specification
SOURCE/HOST: Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human NALP1 (aa 1-1429).
QUANTITY: 100µl corresponding to 2 x 106 cells.
FORMULATION: Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION: Positive control in Western blot for MAb to NALP1 (human) (Nalpy1-4) (Prod. No. ALX-804-803), PAb to NALP1 (human) (AL176) (Prod. No. ALX-210-904), PAb to NALP1 (human) (CT) (Bur 242) (Prod. No. ALX-210-017) and PAb to NALP1 (human) (NT) (Bur 241) (Prod. No. ALX-210-018).
Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products
 
 
ALX-840-604 Revised 01-Mar-07
Positive Control (Cell Lysate) for Antibodies to RIP2 (human)
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SYNONYMS Positive Control (Cell Lysate) for Antibodies to Receptor-interacting Protein 2 (human)
Positive Control (Cell Lysate) for Antibodies to CARDIAK (human)
Positive Control (Cell Lysate) for Antibodies to CARD-containing IL-1β Converting Enzyme Associated Kinase (human)
Positive Control (Cell Lysate) for Antibodies to RICK (human)
Positive Control (Cell Lysate) for Antibodies to RIPK2 (human)
Positive Control (Cell Lysate) for Antibodies to Receptor-interacting Serine-Threonine Kinase 2 (human)
PRODUCT LINE Immunology
PRODUCT CATEGORY Positive Controls (Cell Lysates & Extracts)
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-840-604-R100   100 µl 70.00 USD Add To Cart
Product Specification
SOURCE/HOST: Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human RIP2 (receptor-interacting protein 2) (aa 1-541) fused at the C-terminus to a FLAG®-tag.
QUANTITY: 100µl corresponding to 2x106 cells.
FORMULATION: Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION: Positive control in Western blot for MAb to RIP2 (human) (Nick-1) (Prod. No. ALX-804-139). Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Nod-complex / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products
 
 
ALX-840-606 Revised 08-Mar-07
Positive Control (Cell Lysate) for Antibodies to CARMA1 (human)
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SYNONYMS Positive Control (Cell Lysate) for Antibodies to CARD-containing MAGUK Protein 1 (human)
Positive Control (Cell Lysate) for Antibodies to CARD11 (human)
Positive Control (Cell Lysate) for Antibodies to Caspase Recruitment Domain-containing Protein 11 (human)
Positive Control (Cell Lysate) for Antibodies to Bimp3 (human)
PRODUCT LINE Immunology
PRODUCT CATEGORY Positive Controls (Cell Lysates & Extracts)
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-840-606-R100   100 µl 70.00 USD Add To Cart
Product Specification
SOURCE/HOST: Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human CARMA1 (CARD-containing MAGUK protein 1) (aa 1-1147) fused at the C-terminus to a FLAG®-tag.
QUANTITY: 100µl corresponding to 2x105 cells.
FORMULATION: Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION: Positive control in Western blot for PAb to CARMA1 (AL220) (Prod. No. ALX-210-903). Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins / Related Products
 
 
ALX-840-609 Revised 03-May-07
Positive Control (Cell Lysate) for Antibodies to RIG-I (human)
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SYNONYMS Positive Control (Cell Lysate) for Antibodies to RIG-1 (human)
Positive Control (Cell Lysate) for Antibodies to Retinoic Acid-inducible Gene 1 (human)
Positive Control (Cell Lysate) for Antibodies to DEAD-box Protein 58 (human)
Positive Control (Cell Lysate) for Antibodies to Probable ATP-dependent RNA Helicase DDX58 (human)
PRODUCT LINE Immunology
PRODUCT CATEGORY Positive Controls (Cell Lysates & Extracts)
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-840-609-R100   100 µl 70.00 USD Add To Cart
Product Specification
SOURCE/HOST: Whole cell lysate of HEK293T cells transfected with an expression plasmid for human RIG-I (aa 1-925).
QUANTITY: 100µl corresponding to 2x105 cells.
PURITY: ≥95%
FORMULATION: Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION: Positive control in Western blot for MAb to RIG-I (human) (Alme-1)
(ALX-804-849) and PAb to RIG-I (human) (AT111) (Prod. No. ALX-210-932). Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins / Related Products
 
 
ALX-803-307 Revised 28-Jul-08
Monoclonal Antibody to Nod2 (human) (2D9)
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SYNONYMS anti-CARD15 (human) MAb (2D9)
anti-Caspase Recruitment Domain-containing Protein 15 (human) MAb (2D9)
anti-Ibd1 (human) MAb (2D9)
anti-Inflammatory Bowel Disease Protein 1 (human) PAb (2D9)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Nod-complex / Related Products
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ALX-803-307-1   1 Vial 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 2D9
ISOTYPE: Mouse IgG1κ
CONCENTRATION: 1mg/ml
PURITY DETAIL: Affinity-purified.
FORMULATION: Liquid. In 200μl PBS, pH 7.2, containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human Nod2 (CARD15) (aa 28-301).
SPECIFICITY: Recognizes human Nod2.
APPLICATION: Immunohistochemistry (2µg/ml)
Immunoprecipitation
Western Blot (transfected cells: 1:100 - 1:250)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least one year after receipt when stored at +4°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Literature References
Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-kappaB: J. Bertin, et al.; J. Biol. Chem. 274, 12955 (1999) Abstract
Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB: N. Inohara, et al. ; J. Biol. Chem. 274, 14560 (1999) Abstract
An induced proximity model for NF-kappa B activation in the Nod1/RICK and RIP signaling pathways  : N. Inohara, et al. ; J. Biol. Chem. 275, 27823 (2000) Abstract
Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB: Y. Ogura, et al.; J. Biol. Chem. 276, 4812 (2001) Abstract
Induction of Nod2 in myelomonocytic and intestinal epithelial cells via nuclear factor-kappa B activation: O. Gutierrez, et al. ; J. Biol. Chem. 277, 41701 (2001) Abstract
A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease: Y. Ogura, et al. ; Nature 411, 603 (2001) Abstract
Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease: J.P. Hugot, et al. ; Nature 411, 599 (2001) Abstract
Human Nod1 confers responsiveness to bacterial lipopolysaccharides  : N. Inohara, et al. ; J. Biol. Chem. 276, 2551 (2001) Abstract
Expression of NOD2 in Paneth cells: a possible link to Crohn's ileitis: Y. Ogura, et al. ; Gut 52, 1591 (2003) Abstract
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • Monoclonal Antibodies
 
 
ALX-804-134 Revised 01-Mar-07
Monoclonal Antibody to Bcl-10 (Carmen-1)
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SYNONYMS anti-CARMEN MAb (Carmen-1)
anti-CIPER MAb (Carmen-1)
anti-CLAP MAb (Carmen-1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Caspase Recruitment Domain [CARD] Proteins / Related Products
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ALX-804-134-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Carmen-1
ISOTYPE: Rat IgM
SOURCE/HOST: Purified from concentrated hybridoma cell culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: >95% (SDS-PAGE)
PURITY DETAIL: Purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human Bcl-10.
SPECIFICITY: Recognizes human and mouse Bcl-10.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY:

Stable for at least 6 months after receipt when stored undiluted at +4°C.
HANDLING: Do not freeze/thaw.
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
NF-kB Pathway Other Products • Innate Immunity Other Products • Monoclonal Antibodies
 
 
ALX-804-139 Revised 09-May-07
Monoclonal Antibody to RIP2 (human) (Nick-1)
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SYNONYMS anti-Receptor-interacting Protein 2 (human) MAb (Nick-1)
anti-CARDIAK (human) MAb (Nick-1)
anti-CARD-containing Interleukin-1β Converting Enzyme Associated Kinase (human) MAb (Nick-1)
anti-RICK (human) MAb (Nick-1)
anti-RIPK2 (human) MAb (Nick-1)
anti-Receptor-interacting Serine/Threonine Protein Kinase 2 (human) MAb (Nick-1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY RIP Kinases
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ALX-804-139-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Nick-1
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIP2 (receptor-interacting protein 2) (aa 1-322).
SPECIFICITY: Recognizes human RIP2.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-604.
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Product Specific Literature References
Participation of Rip2 in LPS signaling is independent of its kinase activity: C. Lu, et al.; J. Biol. Chem. 280, 16278 (2005) Abstract; Full Text
PIDD mediates NF-kB activation in Response to DNA damage: Janssens, et al.; Cell 123, 1079 (2005) Abstract
Participation of Rip2 in lipopolysaccharide signaling is independent of its kinase activity: C. Lu, et al.; J. Biol. Chem. 280, 16278 (2005) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Nod-complex / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Monoclonal Antibodies
 
 
ALX-804-348 Revised 18-Sep-07
Monoclonal Antibody to Apaf-1 (human) (2E12)
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SYNONYMS anti-Apoptosis Protease-activating Factor 1 (human) MAb (2E12)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Apaf-1
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ALX-804-348-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 2E12
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human Apaf-1 (apoptosis protease-activating factor 1) N-terminal CARD and CED-4 homologous domains (aa 1-464) .
SPECIFICITY: Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.
APPLICATION: ELISA
Immunocytochemistry
Immunohistochemistry (EM on frozen sections; for details see G. Hausmann, et al., below.)
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Bcl-2 family members do not inhibit apoptosis by binding the caspase activator Apaf-1: K. Moriishi, et al.; PNAS 96, 9683 (1999) Abstract; Full Text
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell Biol. 149, 623 (2000) Abstract; Full Text
Anti-Fas antibody-induced apoptosis and its signal transduction in human gastric carcinoma cell lines: K. Adachi, et al.; Int. J. Oncol. 23, 713 (2003) Abstract
Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein: D.H. Cho, et al.; J. Biol. Chem. 279, 39942 (2004) Abstract; Full Text
BOK and NOXA Are Essential Mediators of p53-dependent Apoptosis: A.G. Yakovlev, et al.; J. Biol. Chem. 279, 28367 (2004) Abstract
General Literature References
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997) Abstract
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997) Abstract
Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development: F. Cecconi, et al.; Cell 94, 727 (1998) Abstract
Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation: Y. Hu, et al.; PNAS 95, 4386 (1998) Abstract; Full Text
Apaf1 in developmental apoptosis and cancer: how many ways to die?: F. Cecconi & P. Gruss; Cell. Mol. Life Sci. 58, 1688 (2001), (Review) Abstract
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • Monoclonal Antibodies
 
 
ALX-804-349 Revised 18-Sep-07
Monoclonal Antibody to Apaf-1 (13F11)
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SYNONYMS anti-Apoptosis Protease-activating Factor 1 MAb (13F11)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Apaf-1
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ALX-804-349-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
CLONE: 13F11
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse Apaf-1 (apoptosis protease-activating factor 1) (aa 1-97) containing the N-terminal CARD domain.
SPECIFICITY: Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1.
APPLICATION: Immunocytochemistry
Immunoprecipitation
Western Blot
SHIPPING: