ALEXIS Biochemicals: Polyclonal Antibody to Cardif (human) (AT107)

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ALX-804-715

Revised 19-Jun-08

Monoclonal Antibody to ACE2 (human) (AC18F)
SYNONYMS	anti-Angiotensin-converting Enzyme 2 (human) MAb (AC18F) anti-SARS Receptor (human) MAb (AC18F)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Angiotensin Converting Enzyme [ACE] & ACE2/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-715-C050		50 µg	195.00 USD
ALX-804-715-C100		100 µg	270.00 USD
ALX-804-715B-C050	Biotin	50 µg	380.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	AC18F
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:	Recombinant human ACE2 (angiotensin-converting enzyme 2).
SPECIFICITY:	Recognizes human ACE2.
APPLICATION:	ELISA (direct and indirect: 1:2’000-1:10’000) Flow Cytometry Western Blot (1:2’000-1:10’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

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Product Specific Literature References

Co-localization of angiotensin-converting enzyme 2-, octomer-4- and CD34 positive cells in rabbit atherosclerotic plaques: A. Zulli, et al.; Exp. Physiol. 93, 564 (2008) Abstract

General Information

MANUFACTURER

Manufactured by AdipoGen, Inc.

Further Categories Containing This Product:

SARS Coronavirus/Related Products • Monoclonal Antibodies

ALX-804-847

Revised 19-Jun-08

Monoclonal Antibody to Cardif (human) (Adri-1)
SYNONYMS	anti-CARD Adapter Inducing Interferon-β (human) MAb (Adri-1) anti-IPS-1 Cardif (human) MAb (Adri-1) anti-Interferon-β Promoter Stimulator Protein 1 (human) MAb (Adri-1) anti-MAVS (human) MAb (Adri-1) anti-Mitochondrial Antiviral Signalling Protein (human) MAb (Adri-1) anti-VISA (human) MAb (Adri-1) anti-Virus-induced Signalling Adapter (human) MAb (Adri-1)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Antiviral Signalling/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-847-C100		100 µg	370.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	Adri-1
ISOTYPE:	Mouse IgG2b
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).
SPECIFICITY:	Recognizes human Cardif.
APPLICATION:	Immunocytochemistry (1:100) Immunohistochemistry (paraffin sections) Immunoprecipitation (1:200) Western Blot (1:1’000)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

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Product Specific Literature References

TRADD Protein Is an Essential Component of the RIG-like Helicase Antiviral Pathway: M.C. Michallet, et al.; Immunity 28, 651 (2008) Abstract

General Information

RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons.
Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005) Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005) Abstract
CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006) Abstract

Further Categories Containing This Product:

NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins/Related Products • Polyclonal Antibodies

ALX-804-278

Revised 31-Oct-06

Monoclonal Antibody to Hepatitis Bx Protein (X36C)
SYNONYMS	anti-HBx Protein MAb (X36C)
PRODUCT LINE	Viral Signalling
PRODUCT CATEGORY	Hepatitis Viruses/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-278-C100		100 µg	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Others
CLONE:	X36C
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:	Recombinant HBx protein (hepatitis Bx protein).
SPECIFICITY:	Recognizes HBx. Detects a band of ~17kDa by Western blot.
APPLICATION:	ELISA Immunoprecipitation (does not immunoprecipitate HBx complexed with XIP) Western Blot (1µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Further Categories Containing This Product:

Monoclonal Antibodies

ALX-804-277

Revised 14-Dec-06

Monoclonal Antibody to Hepatitis C Core Protein (C7-50)
PRODUCT LINE	Viral Signalling
PRODUCT CATEGORY	Hepatitis Viruses/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-277-C100		100 µg	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Others
CLONE:	C7-50
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:	Purified HCV (hepatitis C virus) core fusion protein (genotype 1b).
SPECIFICITY:	Recognizes epitope between aa 21-40 of HCV core protein. This sequence is conserved among different HCV strains. Detects a band of ~21kDa by Western blot.
APPLICATION:	ELISA Immunocytochemistry Immunoprecipitation Western Blot (1µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Characterization of three novel monoclonal antibodies against hepatitis C virus core protein: D. Moradpour, et al.; J. Med. Virol. 48, 234 (1996) Abstract

General Information

Hepatitis C virus (HCV) is the primary cause of non-A, non-B hepatitis and results in most HCV-infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. The 21kDa core protein of HCV is well conserved among different HCV genotypes and may suppress hepatitis B virus replication in a phosphorylation dependent manner.
BACKGROUND/TECHNICAL INFORMATION	Immunocytochemical staining of HCV core protein in transfected chimp hepatocytes yields a staining pattern consistent with cytoplasmic and vesicular staining.

Further Categories Containing This Product:

Monoclonal Antibodies

ALX-804-863

Revised 29-Jan-08

Monoclonal Antibody to MDA5 (human) (Hely-1)
SYNONYMS	anti-Melanoma Differentiation-associated Gene 5 (human) MAb (Hely-1) anti-Helicard (human) MAb (Hely-1)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	RNA Helicases/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-863-C100		100 µg	380.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	Hely-1
ISOTYPE:	Mouse IgG1
SOURCE/HOST:	Purified from concentrated hybridoma tissue cell culture supernatant.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555).
SPECIFICITY:	Recognizes human MDA5.
APPLICATION:	ELISA Immunoprecipitation (1:100) Western Blot (1:1‘000)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract
Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses: H. Kato, et al.; Nature 441, 101 (2006) Abstract

Further Categories Containing This Product:

NF-kB Pathway Other Products • Antiviral Signalling/Related Products • Caspase Recruitment Domain [CARD] Proteins/Related Products • Interferons & Interferon Receptors Other Products • Monoclonal Antibodies

ALX-803-059

Revised 27-Apr-06

Monoclonal Antibody to NS3 (HCV) (1B6)
SYNONYMS	anti-Nonstructural Protein 3 (HCV) MAb (1B6)
PRODUCT LINE	Viral Signalling
PRODUCT CATEGORY	Hepatitis Viruses/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-803-059-R100		100 µl	360.00 USD

Product Specification

CLONE:	1B6
ISOTYPE:	Mouse IgG1
PURITY:	Liquid. Ascites fluid diluted in PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant hepatitis C virus (HCV) NS3 serine protease domain.
SPECIFICITY:	Recognizes HCV NS3 (epitope mapped to NS3 aa 160-193), recognizes NS3 alone or the NS3-4A complex.
APPLICATION:	Immunocytochemistry (1:50) Western Blot (1:500)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Subcellular localization, stability, and trans-cleavage competence of the hepatitis C virus NS3-NS4A complex expressed in tetracycline-regulated cell lines: B. Wolk, et al.; J. Virol. 74, 2293 (2000) Abstract; Full Text
Protein-protein interactions between hepatitis C virus nonstructural proteins: Dimitrova, et al.; J. Virol. 77, 5401 (2003) Abstract; Full Text
Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons: R. Gosert, et al.; J. Virol. 77, 5487 (2003) Abstract; Full Text
Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes: D. Moradpour, et al.; J. Virol. 78, 7400 (2004) Abstract; Full Text
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids: S.B. Kapadia and F.V. Chisari; PNAS 102, 2561 (2005) Abstract; Full Text
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract

General Information

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Unravelling hepatitis C virus replication from genome to function: B.D. Lindenbach and C.M. Rice; Nature 436, 933 (2005) Abstract

Further Categories Containing This Product:

Monoclonal Antibodies

ALX-803-061

Revised 26-Apr-07

Monoclonal Antibody to NS5B (HCV) (5B-12B7)
SYNONYMS	anti-Nonstructural Protein 5B (HCV) MAb (5B-12B7)
PRODUCT LINE	Viral Signalling
PRODUCT CATEGORY	Hepatitis Viruses/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-803-061-R100		100 µl	360.00 USD

Product Specification

CLONE:	5B-12B7
ISOTYPE:	Mouse IgG2a
PURITY:	Liquid. Ascites fluid diluted in PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase.
SPECIFICITY:	Recognizes HCV NS5B (epitope mapped to NS5B aa 139-392).
APPLICATION:	Immunocytochemistry (1:100) Immunoprecipitation Functional Application: Blocks the RNA-dependent RNA polymerase activity in vitro. Note: Not suitable for Western blot. Use MAb to NS5B (HCV) (5B-3B1) (Prod. No. ALX-803-060).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Determinants for membrane association of the hepatitis C virus RNA-dependent RNA polymerase: J. Schmidt-Mende, et al.; J. Biol. Chem. 276, 44052 (2001) Abstract; Full Text
Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase: D. Moradpour, et al.; J. Biol. Chem. 277, 593 (2002) Abstract; Full Text
Protein-protein interactions between hepatitis C virus nonstructural proteins: Dimitrova, et al.; J. Virol. 77, 5401 (2003) Abstract; Full Text
Interference of hepatitis C virus RNA replication by short interfering RNAs: Kapadia, et al.; PNAS 100, 2014 (2003) Abstract; Full Text

General Information

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Unravelling hepatitis C virus replication from genome to function: B.D. Lindenbach and C.M. Rice; Nature 436, 933 (2005) Abstract

CLONE:	5B-3B1
ISOTYPE:	Mouse IgG2b
FORMULATION:	Liquid. Ascites fluid diluted in PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase.
SPECIFICITY:	Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382).
APPLICATION:	Western Blot (1:1'000)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

SPECIES CROSSREACTIVITY:	Human Mouse Dog
CLONE:	8C10
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN:	Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1.
SPECIFICITY:	Recognizes human, mouse and dog Pin1. Detects a band of ~16 kDa by Western blot.
APPLICATION:	Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL INCLUDED:	Yes (Prod. No. ALX-840-030).