ALEXIS Biochemicals: p53 (human) ELISA Kit

Advanced Search

You are here: Product Lines > Cell Cycle > p53 / Related Products > p53 Other Products

ALX-201-221

Revised 02-Oct-06

Pin1 (human) (recombinant)
SYNONYMS	Peptidyl-prolyl cis-trans Isomerase Pin1 (human) (recombinant) PPIase Pin1 (human) (recombinant) Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 (human) (recombinant) PPIase NIMA-interacting 1 (human) (recombinant) Rotamase Pin1 (human) (recombinant)
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Alzheimer Research Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-221-C100		100 µg	325.00 USD

Product Specification

MW:	~18.2kDa
SOURCE/HOST:	Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1 produced in E. coli.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 20mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 5mM DTT and 20% glycerol.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Further Categories Containing This Product:

Stress & Heat Shock Proteins Other Products • Antiviral Signalling / Related Products • Recombinant Proteins / Fusion Proteins • p53 Other Products

ALX-201-426

Revised 24-Jul-08

Sirtuin 1 (human) (recombinant) (His)
SYNONYMS	Sirt1 (human) (recombinant) (His) SIR2-like Protein 1 (human) (recombinant) (His)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Sirtuins / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-426-C010		10 µg	300.00 USD
ALX-201-426-C050		50 µg	470.00 USD

Product Specification

SOURCE/HOST:	Produced in E. coli. The mature peptide of human sirtuin 1 (aa 2-555) is fused at the N-terminus to a His-tag.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. 0.2μm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM sodium chloride.
ENDOTOXIN CONTENT:	<1EU/µg protein (LAL-test).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Images

Please click on thumbnails to enlarge.

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q96EB6: SIRT1 (human)
MANUFACTURER	Manufactured by AdipoGen, Inc.

Related Products

ALX-210-961	Polyclonal Antibody to AROS (human) (AT135)
ALX-201-426	Sirtuin 1 (human) (recombinant) (His)

Further Categories Containing This Product:

NAD+ Metabolism / Related Products • Recombinant Proteins / Fusion Proteins • p53 Other Products

ALX-210-419

Revised 30-Oct-06

Polyclonal Antibody to 53BP1
SYNONYMS	anti-Tumor Protein p53 Binding Protein 1 PAb anti-TP53BP1 PAb anti-p202 PAb
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	p53 Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-419-R050		50 µl	180.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	From rabbit.
FORMULATION:	Liquid. Neat serum containing 0.05% sodium azide.
IMMUNOGEN:	Recombinant human 53BP1 (tumor protein p53 binding protein 1) (aa 1308-1824).
SPECIFICITY:	Recognizes native and denatured forms of full-length human 53BP1. Cross-reacts with mouse 53BP1.
APPLICATION:	Immunocytochemistry (1:200); Immunoprecipitation; Western blot (1:2'000).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Storage at +4°C is recommended; if freezing is prefered add 50% glycerol.

Product Images

Please click on thumbnails to enlarge.

Product Description

The 53BP1 protein is implicated in the early response to DNA damage. The residues aa 1308-1824 are central to the antigen sequence recognized by the antibody ALX-210-419.

Product Specific Literature References

Phosphorylation and rapid relocalization of 53BP1 to nuclear foci upon DNA damage: L. Anderson, et al.; Mol. Cell. Biol. 21, 1719 (2001) Abstract
Kinetochore localisation of the DNA damage response component 53BP1 during mitosis: D. Jullien, et al.; J. Cell Sci. 115, 71 (2002) Abstract; Full Text

Further Categories Containing This Product:

DNA Damage Checkpoint Other Products • Polyclonal Antibodies

ALX-210-486

Revised 27-Nov-08

Polyclonal Antibody to Sirtuin 1 (human)
SYNONYMS	anti-Sirt1 (human) PAb anti-SIR2-like Protein 1 (human) PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Sirtuins / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-486-C100		100 µg	340.00 USD

Product Specification

SOURCE/HOST:	From rabbit.
PURITY DETAIL:	Purified IgG.
FORMULATION:	Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:	Recombinant human sirtuin 1.
SPECIFICITY:	Recognizes human sirtuin 1.
APPLICATION:	ELISA (direct and indirect: 1:2’000-1:5’000) Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for up to 6 months when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Images

Please click on thumbnails to enlarge.

General Information

MANUFACTURER

Manufactured by AdipoGen, Inc.

Further Categories Containing This Product:

NAD+ Metabolism / Related Products • Polyclonal Antibodies • p53 Other Products

ALX-210-487

Revised 27-Nov-08

Polyclonal Antibody to Sirtuin 2 (human)
SYNONYMS	anti-Sirt2 (human) PAb anti-SIR2-like Protein 2 (human) PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Sirtuins / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-487-C100		100 µg	340.00 USD

Product Specification

SOURCE/HOST:	From rabbit.
PURITY DETAIL:	Purified IgG.
FORMULATION:	Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:	Recombinant human sirtuin 2.
SPECIFICITY:	Recognizes human sirtuin 2.
APPLICATION:	ELISA (direct and indirect: 1:2’000-1:5’000) Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for up to 6 months when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Images

Please click on thumbnails to enlarge.

General Information

MANUFACTURER

Manufactured by AdipoGen, Inc.

Further Categories Containing This Product:

NAD+ Metabolism / Related Products • Polyclonal Antibodies • p53 Other Products

ALX-210-768

Revised 11-Apr-06

Polyclonal Antibody to p51A
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	p53 Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-768-C200		200 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	From rabbit.
CONCENTRATION:	2mg/ml
PURITY DETAIL:	Epitope-affinity purified IgG.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 424-446 (E⁴²⁴PRRETPKQSDVFFRHSKPPNRS⁴⁴⁶) of C-terminal human p51A.
SPECIFICITY:	Recognizes human and mouse p51A. Detects a band of ~54kDa by Western blot. Does not cross-react with p51B.
APPLICATION:	Immunocytochemistry (1:300) Immunohistochemistry (paraffin sections) Western Blot (1:1'000)
QUALITY CONTROL:	Western blot on mouse thymus, mouse heart and human T cell line Jurkat.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-165-040.

General Information

p51 is a member of the p53 tumor suppressor gene family, which is induced by DNA damage and/or stress stimuli, causing cells to undergo G1-arrest or apoptotic death. Unlike ubiquitous expression of p53, expression of p51 is restricted to a limited number of tissues. The p51 gene encodes two major splicing variants, p51A (448 aa protein with a molecular weight of 50.9kDa) and p51B (641 aa protein with a molecular weight of 71.9kDa), a human homolog of rat Ket. p51A and p51B differ in their carboxyl-terminal structure.

BACKGROUND/TECHNICAL INFORMATION

Most of the p51A protein in Jurkat and K562 tumor cell lines is degraded.

General Literature References

Cloning and functional analysis of human p51, which structurally and functionally resembles p53: M. Osada, et al.; Nature Med. 4, 839 (1998) Abstract
Human p53-p51 (p53-related) fusion protein: a potent BAX transactivator: S. Ikawa, et al.; Jpn. J. Cancer Res. 90, 596 (1999) Abstract
p53 family genes: structural comparison, expression and mutation: S. Ikawa, et al.; Cell Death Differ. 6, 1154 (1999) Abstract
p53 is a transcriptional activator of the muscle-specific phosphoglycerate mutase gene and contributes in vivo to the control of its cardiac expression: P. Ruiz-Lozano, et al.; Cell Growth Differ. 10, 295 (1999) Abstract; Full Text
Failure of viral oncoproteins to target the p53-homologue p51A: J. Roth & M. Dobbelstein; J. Gen. Virol. 80, 3251 (1999) Abstract; Full Text
Mutational analysis of p51A/TAp63gamma, a p53 homolog, in non-small cell lung cancer and breast cancer: M. Sunahara, et al.; Oncogene 18, 3761 (1999) Abstract

Further Categories Containing This Product:

Polyclonal Antibodies

ALX-210-769

Revised 11-Apr-06

Polyclonal Antibody to p51B
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	p53 Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-769-C200		200 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	From rabbit.
CONCENTRATION:	2mg/ml.
PURITY DETAIL:	Epitope-affinity purified IgG.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 623-641(N⁶²³FDMDARRNKQQRIKEEGE⁶⁴¹) of C-terminal human p51B.
SPECIFICITY:	Recognizes human and mouse p51B. Detects a band of ~75kDA by Western blot. Does not cross-react with p51A.
APPLICATION:	Immunocytochemistry (1:300) Immunohistochemistry (paraffin sections) Western Blot (1:1'000)
QUALITY CONTROL:	Western blot on mouse lung and human K562 cell line. As expected, some of the p51B is degraded in the tumor cell line K562.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-165-041.

General Information

General Literature References

Cloning and functional analysis of human p51, which structurally and functionally resembles p53  : M. Osada, et al.; Nat. Med. 4, 839 (1998) Abstract
Human p53-p51 (p53-related) fusion protein: a potent BAX transactivator  : S. Ikawa, et al.; Jpn. J. Cancer Res. 90, 596 (1999) Abstract
p53 family genes: structural comparison, expression and mutation  : S. Ikawa, et al.; Cell Death Differ. 6, 1154 (1999) Abstract
Failure of viral oncoproteins to target the p53-homologue p51A: J. Roth & M. Dobbelstein; J. Gen. Virol. 80, 3251 (1999) Abstract; Full Text
p53 is a transcriptional activator of the muscle-specific phosphoglycerate mutase gene and contributes in vivo to the control of its cardiac expression  : P. Ruiz-Lozano, et al.; Cell Growth Differ. 10, 295 (1999) Abstract; Full Text
Mutational analysis of p51A/TAp63gamma, a p53 homolog, in non-small cell lung cancer and breast cancer: M. Sunahara, et al.; Oncogene 18, 3761 (1999) Abstract

Further Categories Containing This Product:

Polyclonal Antibodies

ALX-210-947

Revised 13-Oct-08

Polyclonal Antibody to TIGAR (AT122)
SYNONYMS	anti-Tp53-induced Glycolysis and Apoptosis Regulator PAb (AT122)
PRODUCT LINE	Obesity & Adipokines
PRODUCT CATEGORY	Glycobiology Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-947-C100		100 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml
PURITY DETAIL:	Protein A-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human TIGAR (aa 1-270) fused at the N-terminus to a tag.
SPECIFICITY:	Recognizes human and mouse TIGAR.
APPLICATION:	Western Blot (1:1’000) Note: Does not work for immunoprecipitation.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Images

Please click on thumbnails to enlarge.

General Information

The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. TIGAR (TP53-induced glycolysis and apoptosis regulator) has been identified as a novel p53-inducible gene. TIGAR expression decreased fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis. In addition, TIGAR expression lowered intracellular reactive oxygen species (ROS) levels and protected cells from p53-induced apoptosis. Bensaad et al., proposed that TIGAR modulates the apoptotic response to p53, thereby allowing cells to survive mild or transient stress.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

TIGAR, a p53-inducible regulator of glycolysis and apoptosis: K. Bensaad, et al.; Cell 126, 107 (2006) Abstract; Full Text

ALX-201-430	Sirtuin 2 (human) (recombinant) (His)
ALX-210-961	Polyclonal Antibody to AROS (human) (AT135)

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q8IXJ6: SIRT2 (human)
MANUFACTURER	Manufactured by AdipoGen, Inc.

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml
PURITY DETAIL:	Protein A-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human AROS (aa 2-136)
SPECIFICITY:	Recognizes human AROS.
APPLICATION:	Western blot (1:1’000)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: