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Viral Signalling
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ALX-746-023 Revised 11-Sep-08 New product
Oligo(deoxyadenosine:deoxythymidine) (endotoxin-free) (synthetic)
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SYNONYMS Oligo(dA:dT) (endotoxin-free) (synthetic)
PRODUCT LINE Immunology
PRODUCT CATEGORY Antiviral Signalling / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-746-023-C050   50 µg 250.00 USD Add To Cart
Product Specification
SOURCE/HOST: Synthetic.
QUANTITY: Aliquoted by weight of dry material. Contains polymer, residual salt and water. Sufficient for at least 200 cellular activation assays at 0.01-0.025µg/ml.
FORMULATION: Lyophilized. Sterile.
ENDOTOXIN CONTENT: <0.02EU/µg (LAL test; BioWhittaker)
RECONSTITUTION: For a 250µg/ml stock solution, dissolve the total vial content in 200µl endotoxin-free buffer or water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Aqueous stock solution is stable for 1 day when stored at +4°C.
HANDLING: Protect from light. After reconstitution, prepare aliquots and store at -20°C. For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Product Description
Strong IFN inducer, dependent of IRF3.
General Information
BACKGROUND/TECHNICAL INFORMATION For in vitro stimulation we recommend the following procedure:
1. Bone marrow-derived mouse dendritic cells (BM mDC) are cultured 8x104/100μl in RPMI containing
10% FCS over night at 5% CO2 and 37°C.
2. The medium is removed and replaced by 100μl OPTI MEM (Invitrogen) and subsequently
the transfection mixture is added: 25μl OPTI MEM containing 0.025 to 0.25 Poly(dA:dT) + 25μl OPTI MEM
containing 0.5μl Lipofectamine 2000 (Invitrogen) or alternative transfection agents.
3. Over night stimulation at 5% CO2 and 37°C.
4. Measurement of IFN-α/β in culture supernatants.
General Literature References
DAI (DLM-1/ZBP1) is a cytosolic DNA sensor and an activator of innate immune response: A. Takaoka, et al.; Nature 448, 501 (2007) Abstract
Cytosolic DNA recognition for triggering innate immune responses: A. Takaoka & T. Taniguchi; Adv. Drug Deliv. Rev. 60, 847 (2008) Abstract
Intracellular pattern-recognition receptors: C. Dostert, et al.; Adv. Drug. Deliv. Rev. 60, 830 (2008) Abstract
The inflammasome recognizes cytosolic microbial and host DNA and triggers an innate immune response: D.A. Muruve, et al.; Nature 452, 103 (2008) Abstract
Related Products
 
 
ALX-201-221 Revised 02-Oct-06
Pin1 (human) (recombinant)
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SYNONYMS Peptidyl-prolyl cis-trans Isomerase Pin1 (human) (recombinant)
PPIase Pin1 (human) (recombinant)
Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 (human) (recombinant)
PPIase NIMA-interacting 1 (human) (recombinant)
Rotamase Pin1 (human) (recombinant)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Alzheimer Research Other Products
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ALX-201-221-C100   100 µg 325.00 USD Add To Cart
Product Specification
MW: ~18.2kDa
SOURCE/HOST: Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1 produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 20mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 5mM DTT and 20% glycerol.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Stress & Heat Shock Proteins Other Products • Antiviral Signalling / Related Products • Recombinant Proteins / Fusion Proteins • p53 Other Products
 
 
ALX-210-444 Revised 25-Jul-07
Polyclonal Antibody to ACE2 (human)
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SYNONYMS anti-Angiotensin-converting Enzyme 2 (human) PAb
anti-SARS Receptor (human) PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin Converting Enzyme [ACE] & ACE2 / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-444-C100   100 µg 340.00 USD Add To Cart
ALX-210-444B-C050 Biotin 50 µg 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Purified IgG.
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human ACE2 (angiotensin-converting enzyme 2).
SPECIFICITY: Recognizes human ACE2.
APPLICATION: ELISA (direct and indirect: 1:2’000-1:10’000)
Western Blot 1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
SARS Coronavirus / Related Products • Polyclonal Antibodies
 
 
ALX-210-929 Revised 16-Apr-08
Polyclonal Antibody to Cardif (human) (AT107)
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SYNONYMS anti-CARD Adapter Inducing Interferon-β (human) PAb (AT107)
anti-IPS-1 (human) PAb (AT107)
anti-Interferon-β Promoter Stimulator Protein 1 (human) PAb (AT107)
anti-MAVS (human) PAb (AT107)
anti-Mitochondrial Antiviral Signalling Protein (human) PAb (AT107)
anti-VISA (human) PAb (AT107)
anti-Virus-induced Signalling Adapter (human) PAb (AT107)
PRODUCT LINE Immunology
PRODUCT CATEGORY Antiviral Signalling / Related Products
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ALX-210-929-C100   100 µg 370.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).
SPECIFICITY: Recognizes human cardif.
APPLICATION: Immunocytochemistry (1:500)
Immunoprecipitation (1:100)
Western Blot (1:2’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Images
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Product Specific Literature References
Regulation of antiviral responses by a direct and specific interaction between TRAF3 and Cardif : S. K. Saha; EMBO J. 25, 3257 (2006) Abstract
Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008) Abstract
General Information
RIG-I (retinoic acid-inducible gene I; Ddx58) and Mda5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons.
Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and Mda5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005) Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005) Abstract
CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006) Abstract
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • NF-kB Pathway Other Products • Polyclonal Antibodies
 
 
ALX-210-963 Revised 27-Nov-08 New product
Polyclonal Antibody to LGP2 (human) (AT137)
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SYNONYMS anti-Probable ATP-dependent RNA Helicase DHX58 (human) PAb (AT137)
anti-Probable ATP-dependent RNA Helicase LGP2 (human) PAb (AT137)
anti-Protein D11Lgp2 Homolog (human) PAb (AT137)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
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ALX-210-963-C050   50 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to 15 aa near the C-terminus of human LGP2.
SPECIFICITY: Recognizes human LGP2.
APPLICATION: Immunoprecipitation (1:200)
Western blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
MDA5 and RIG-I are two key intracellular receptors that recognize virus infection to produce type I IFN. LGP2 is homologous to MDA5 and RIG-I, but lacks the caspase recruitment domain (CARD) crucial for their antiviral activity [1]. LGP2 may be a negative regulator of host innate immune defense against viruses by acting through dsRNA sequestration or inhibition of RIG-I dimerization [2].
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity: M. Yoneyama, et al.; J. Immunol. 175, 2851 (2005) Abstract; Full Text
[2] Negative regulation of cytoplasmic RNA-mediated antiviral signaling: A. Komuro, et al.; Cytokine 43, 350 (2008) Abstract
Further Categories Containing This Product:
Antiviral Signalling / Related Products • Polyclonal Antibodies
 
 
ALX-210-935 Revised 20-Feb-08
Polyclonal Antibody to MDA5 (human) (AT113)
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SYNONYMS anti-Melanoma Differentiation-associated Gene 5 (human) PAb (AT113)
anti-Helicard (human) PAb (AT113)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
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ALX-210-935-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555).
SPECIFICITY: Recognizes human MDA5.
APPLICATION: Immunoprecipitation (1:100)
Western Blot (1:1‘000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008) Abstract
General Information
MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract
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Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • NF-kB Pathway Other Products • Interferons & Interferon Receptors Other Products • Antiviral Signalling / Related Products • Polyclonal Antibodies
 
 
ALX-210-352 Revised 30-Oct-06
Polyclonal Antibody to MDA5 (mouse) (AL180)
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SYNONYMS anti-Melanoma Differentiation-associated Gene 5 (mouse) PAb (AL180)
anti-Helicard (mouse) PAb (AL180)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
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ALX-210-352-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Contains 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse MDA5 (melanoma differentiation-associated gene 5) (aa 2-208).
SPECIFICITY: Recognizes mouse MDA5.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Overexpression of Helicard, a CARD-Containing Helicase Cleaved during Apoptosis, Accelerates DNA Degradation: M. Kovacsovics, et al.; Curr. Biol. 12, 838 (2002) Abstract
General Information
MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract
Related Products
Further Categories Containing This Product:
NF-kB Pathway Other Products • Antiviral Signalling / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • Polyclonal Antibodies
 
 
ALX-210-932 Revised 28-Feb-07
Polyclonal Antibody to RIG-I (human) (AT111)
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SYNONYMS anti-RIG-1 (human) PAb (AT111)
anti-Retinoic Acid-inducible Gene 1 Protein (human) PAb (AT111)
anti-DEAD-box Protein 58 (human) PAb (AT111)
anti-Probable ATP-dependent RNA Helicase DDX58 (human) PAb (AT111)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
Ordering Information
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ALX-210-932-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIG-I (retinoic acid-inducible gene 1 protein) (aa 201-713).
SPECIFICITY: Recognizes human RIG-I.
APPLICATION: Immunoprecipitation (1:100)
Western blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
General Information
RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Virology. Sensing viral RNA amid your own: T. Fujita; Science 314, 935 (2006) Abstract
5’-Triphosphate RNA is the ligand for RIG-I: V. Hornung, et al.; Science 314, 994 (2006) Abstract
RIG-I-mediated antiviral responses to single-stranded RNA bearing 5’-phosphates: A. Pichlmair, et al.; Science 314, 997 (2006) Abstract
Related Products
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • NF-kB Pathway Other Products • Antiviral Signalling / Related Products • Interferons & Interferon Receptors Other Products • Polyclonal Antibodies
 
 
ALX-210-367 Revised 25-Sep-08
Polyclonal Antibody to Toll-like Receptor 3 (mouse)
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SYNONYMS anti-TLR3 (mouse) PAb
anti-CD283 (mouse) PAb
PRODUCT LINE Inflammation
PRODUCT CATEGORY TLR Antibodies
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ALX-210-367-R200   200 µl 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptides corresponding to aa 135-150, 828-844 and 876-891 of mouse TLR3 (Toll-like receptor 3).
SPECIFICITY: Recognizes mouse TLR3. Does not cross