ALEXIS Biochemicals: Polyclonal Antibody to Fatty Acid Amide Hydrolase

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ALX-270-022

Revised 09-Jan-08

AEBSF . hydrochloride
SYNONYMS	4-(2-Aminoethyl)benzenesulfonylfluoride . HCl Pefabloc^®SC
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Proteases Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-022-M050		50 mg	40.00 USD
ALX-270-022-M250		250 mg	145.00 USD
ALX-270-022-G001		1 g	360.00 USD

Product Specification

FORMULA:	C₈H₁₀FNO₂S . HCl
MW:	203.2 . 36.5
CAS NUMBER:	30827-99-7
PURITY:	≥98% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in water or 100% ethanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HANDLING:	Protect from moisture.

Product Description

Specific irreversible inhibitor of serine proteases including trypsin, chymotrypsin, plasmin, plasma kallikrein, and thrombin. Stable, non-toxic alternative to PMSF (Prod. No. ALX-270-184). Inhibitor of NAD(P)H oxidase. Activator of MAP kinases JNK and p38.

Product Specific Literature References

Irreversible enzyme inhibitors. 180. Irreversible inhibitors of the C'la component of complement derived from m-(phenoxypropoxy)benzamidine and phenoxyacetamide: B.R. Baker & H.J. Cory; J. Med. Chem. 14, 119 (1971) Abstract
Serine protease inhibitors block priming of monocytes for enhanced release of superoxide: P. Megyeri, et al.; Immunology 86, 629 (1995) Abstract; Full Text
Inhibition of amyloid beta-protein production in neural cells by the serine protease inhibitor AEBSF: M. Citron, et al.; Neuron 17, 171 (1996) Abstract
Lysis of leukemic cells by human macrophages: inhibition by 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), a serine protease inhibitor: Y. Nakabo & M.J. Pabst; J. Leukoc. Biol. 60, 328 (1996) Abstract; Full Text
Inhibition of NADPH oxidase activation by 4-(2-aminoethyl)-benzenesulfonyl fluoride and related compounds: V. Diatchuk, et al.; J. Biol. Chem. 272, 13292 (1997) Abstract; Full Text
4-(2-Aminoethyl)benzenesulfonyl fluoride attenuates tumor-necrosis-factor-alpha-induced blood-brain barrier opening: P. Megyeri, et al.; Eur. J. Pharmacol. 374, 207 (1999) Abstract
Evaluation of two inhibitors of invasion: LY311727 [3-(3-acetamide-1-benzyl-2-ethyl-indolyl-5-oxy)propane phosphonic acid] and AEBSF [4-(2-aminoethyl)-benzenesulphonyl fluoride] in acute murine toxoplasmosis: R. Buitrago-Rey, et al.; J. Antimicrob. Chemother. 49, 871 (2002) Abstract; Full Text
Heme oxygenase-1 gene activation by the NAD(P)H oxidase inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride via a protein kinase B, p38-dependent signaling pathway in monocytes: N. Wijayanti, et al.; J. Biol. Chem. 280, 21820 (2005) Abstract; Full Text
Inhibitory effect of 4-(2-aminoethyl)-benzenesulfonyl fluoride, a serine protease inhibitor, on PI3K inhibitor-induced CHOP expression: T. Hosoi, et al.; Eur. J. Pharmacol. 554, 8 (2007) Abstract

Further Categories Containing This Product:

Heme Oxygenase / Related Products • MAPK Pathway Activators • Cell Stress, Oxidative Stress & Redox Signalling Other Products • Fatty Acid Amide Hydrolase [FAAH] / Related Products

ALX-270-184

Revised 26-May-08

Phenylmethylsulfonyl fluoride
SYNONYMS	PMSF Benzylsulfonyl fluoride
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Proteases Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-184-G001		1 g	25.00 USD
ALX-270-184-G005		5 g	50.00 USD
ALX-270-184-G025		25 g	135.00 USD

Product Specification

FORMULA:	C₇H₇FO₂S
MW:	174.2
CAS NUMBER:	329-98-6
RTECS:	XT8040000
PURITY:	≥99% (HPLC)
APPEARANCE:	White to off-white needle crystals.
SOLUBILITY:	Soluble in 100% ethanol, methanol or isopropanol; insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+20°C
HAZARD:	CORROSIVE. HARMFUL.

Product Description

Irreversible inhibitor of serine proteases. Widely used fatty acid amid hydrolase (FAAH) inhibitor for pretreatment of brain membrane preparations when testing CB₁ receptor activity. Spleen membranes used for CB₂ studies do not require this pretreatment, as FAAH activity is absent in these cells. Inhibits also cysteine proteases like papain (reversible by DTT treatment, Prod. No. ALX-280-001), as well as internucleosomal DNA fragmentation in immature thymocytes. For a related, more stable inhibitor see AEBSF (Prod. No. ALX-270-022).

Product Specific Literature References

Kinetic analysis of differences in brain acetylcholinesterase from fish or mammalian sources: D.E. Moss & D.E. Fahrney; Biochem. Pharmacol. 27, 2693 (1978) Abstract
Inactivation of the protease inhibitor phenylmethylsulfonyl fluoride in buffers: G.T. James; Anal. Biochem. 86, 574 (1978) Abstract
Enzymatic synthesis and degradation of anandamide, a cannabinoid receptor agonist: D.G. Deutsch & S.A. Chin; Biochem. Pharmacol. 46, 791 (1993) Abstract

Further Categories Containing This Product:

Acetylcholine Esterase / Related Products • Fatty Acid Amide Hydrolase [FAAH] / Related Products

ALX-300-146

Revised 20-Jun-08

Palmitoylethanolamide
SYNONYMS	PEA
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Endocannabinoids

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-300-146-M010		10 mg	20.00 USD

Product Specification

FORMULA:	C₁₈H₃₇NO₂
MW:	299.5
CAS NUMBER:	544-31-0
PURITY:	≥99%
APPEARANCE:	White solid.
SOLUBILITY:	Soluble in DMSO, chloroform or THF (30°C).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C

Product Description

Endogenous cannabinoid. Weak ligand of CB₁ (K_i=23.8µM) and CB₂ (K_i=13.9µM) receptor. Inhibits fatty acid amide hydrolase (FAAH) (IC₅₀=5.1µM). Immunosuppressant, anti-inflammatory, anti-nociceptive and anti-convulsant in vivo. The exact mode of action has not yet been revealed. It has been suggested that PEA: i) binds to a yet to be discovered cannabinoid receptor similar to CB₂; ii) administered in vivo elicits the synthesis of endogenous agonists of CB₂; iii) acts as an "entourage" compound by enhancing the activity and/or by influencing the turnover of endogenous agonists of CB₂, possibly but not uniquely, by inhibiting their degradation.

Product Specific Literature References

Mast cells express a peripheral cannabinoid receptor with differential sensitivity to anandamide and palmitoylethanolamide: L. Facci, et al.; PNAS 92, 3376 (1995) Abstract
Biosynthesis of an endogenous cannabinoid precursor in neurons and its control by calcium and cAMP: H. Cadas, et al.; J. Neurosci. 16, 3934 (1996) Abstract
N-(2-hydroxyethyl)hexadecanamide is orally active in reducing edema formation and inflammatory hyperalgesia by down-modulating mast cell activation: S. Mazzari, et al.; Eur. J. Pharmacol. 300, 227 (1996) Abstract
The ALIAmide palmitoylethanolamide and cannabinoids, but not anandamide, are protective in a delayed postglutamate paradigm of excitotoxic death in cerebellar granule neurons: S.D. Skaper, et al.; PNAS 93, 3984 (1996) Abstract
Analogues and homologues of N-palmitoylethanolamide, a putative endogenous CB(2) cannabinoid, as potential ligands for the cannabinoid receptors: D.M. Lambert, et al.; Biochim. Biophys. Acta 1440, 266 (1999) Abstract
Effects of homologues and analogues of palmitoylethanolamide upon the inactivation of the endocannabinoid anandamide: K.O. Jonsson, et al.; Br. J. Pharmacol. 133, 1263 (2001) Abstract
The palmitoylethanolamide family: a new class of anti-inflammatory agents?: D.M. Lambert, et al.; Curr. Med. Chem. 9, 663 (2002) Abstract
Effect on cancer cell proliferation of palmitoylethanolamide, a fatty acid amide interacting with both the cannabinoid and vanilloid signalling systems: L. De Petrocellis, et al.; Clin. Pharmacol. 16, 297 (2002) Abstract
Palmitoylethanolamide, endocannabinoids and related cannabimimetic compounds in protection against tissue inflammation and pain: Potential use in companion animals.
: G. Re, et al.; Vet. J. 173, 21 (2007), Review Abstract

Further Categories Containing This Product:

Anandamide & Anandamide Analogs • Analgesic / Anti-nociceptive Agents / Related Products • Immunomodulators Other Products • Anti-inflammatory Agents Other Products • Fatty Acid Amide Hydrolase [FAAH] / Related Products

ALX-300-302

Revised 25-Feb-05

Palmitoyl-N-isopropylamide
SYNONYMS	PIA N-Isopropyl-hexadecanamide
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Fatty Acid Amide Hydrolase [FAAH] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-300-302-M005		5 mg	63.00 USD

Product Specification

FORMULA:	C₁₉H₃₉NO
MW:	297.5
CAS NUMBER:	189939-61-5
PURITY:	≥98%
APPEARANCE:	Crystalline solid.
SOLUBILITY:	Soluble in ethanol (20mg/ml) and dimethyl formamide (3mg/ml). PIA will be stable for at least 6 months in these solvents when stored at -20°C. PIA is sparingly soluble in aqueous buffers.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 2 years after receipt when stored at -20°C.

Product Description

Inhibitor of fatty acid amide hydrolase (FAAH) (IC₅₀=12.8µM). Displays little binding to CB₁ and CB₂receptors (IC₅₀>100µM) and very weakly blocks anandamide uptake (IC₅₀~100µM).

Product Specific Literature References

Effects of homologues and analogues of palmitoylethanolamide upon the inactivation of the endocannabinoid anandamide: K.O. Jonsson, et al.; Br. J. Pharmacol. 133, 1263 (2001) Abstract
AM404 and VDM 11 non-specifically inhibit C6 glioma cell proliferation at concentrations used to block the cellular accumulation of the endocannabinoid anandamide: K.O. Jonsson, et al.; Arch. Toxicol. 77, 201 (2003) Abstract

Further Categories Containing This Product:

Anandamide Uptake Inhibitors

ALX-340-001

Revised 28-Jul-06

Arachidonyl trifluoromethylketone
SYNONYMS	AACOCF₃
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	PLA2 Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-340-001-M005		5 mg	70.00 USD
ALX-340-001-M010		10 mg	110.00 USD
ALX-340-001-M050		50 mg	390.00 USD

Product Specification

FORMULA:	C₂₁H₃₁F₃O
MW:	356.5
CAS NUMBER:	149301-79-1
PURITY:	≥98%
APPEARANCE:	Clear to yellow oil.
SOLUBILITY:	25mg/ml soluble in 100% ethanol or DMSO.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Dissolve in solvent purged with nitrogen or argon. Store under nitrogen or argon in tightly sealed container. Solutions are stable for up to 1 month when stored under nitrogen or argon in tightly sealed container at -80°C.
HANDLING:	PROTECT FROM AIR!
HAZARD:	TOXIC.

Product Description

Selective inhibitor of both, Ca^2+-dependent and Ca²⁺-independent phospholipase A₂(PLA₂), but not secretory PLA₂(sPLA₂). Also inhibits fatty acid amide hydrolase (FAAH) and anandamide amidase.

Product Specific Literature References

Inhibitors of arachidonoyl ethanolamide hydrolysis: B. Koutek, et al.; J. Biol. Chem. 269, 22937 (1994) Abstract; Full Text

Further Categories Containing This Product:

Fatty Acid Amide Hydrolase [FAAH] / Related Products

ALX-340-017

Revised 11-Jul-08

Methylarachidonyl fluorophosphonate
SYNONYMS	MAFP Methylphosphonofluoridic acid 5,8,11,14-eicosatetraenyl ester
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	PLA2 Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-340-017-M001		1 mg	33.00 USD
ALX-340-017-M005		5 mg	147.00 USD

Product Specification

FORMULA:	C₂₁H₃₆FO₂P
MW:	370.5
CONCENTRATION:	10mg/ml
PURITY:	≥98%
FORMULATION:	Liquid. Solution in methyl acetate.
SOLUBILITY:	3mg/ml soluble in 100% ethanol, DMSO or dimethyl formamide; 0.5mg/ml soluble in a 1:1 solution of ethanol:PBS (pH 7.2) (dilute the ethanol solution with PBS).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stable for at least one year after receipt when stored at -80°C. We do not recommend storing aqueous solutions for more than one day.

Product Description

Selective, active-site directed, irreversible inhibitor of cPLA₂ and iPLA_2.Potent inhibitor of fatty acid amide hydrolase (FAAH) (IC₅₀=2.5nM), 800 -fold more potent than AACOCF₃ (Prod. No. ALX-340-001). Binds to the CB₁ receptor in rat brain membrane (IC₅₀=20nM).

Product Specific Literature References

Methyl arachidonyl fluorophosphonate: a potent irreversible inhibitor of anandamide amidase: D.G. Deutsch, et al.; Biochem. Pharmacol. 53, 255 (1997) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

To change the solvent, evaporate the methyl acetate under a gentle stream of nitrogen and immediately add the solvent of choice.

Further Categories Containing This Product:

Cannabinoid Receptor Agonists & Antagonists / Related Products • Fatty Acid Amide Hydrolase [FAAH] / Related Products

ALX-340-041

Revised 11-Dec-07

Olvanil
SYNONYMS	NE-19550 N-Vanillyloleoylamide
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	TRPV1 Agonists and Antagonists / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-340-041-M005		5 mg	30.00 USD
ALX-340-041-M010		10 mg	48.00 USD

Product Specification

FORMULA:	C₂₆H₄₃NO₃
MW:	417.6
CAS NUMBER:	58493-49-5
PURITY:	≥98%
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in 100% ethanol, chloroform, dimethyl formamide or DMSO.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

"Hybrid" activator of CB₁ receptor (CB₁: K_i=1.6µM; CB₂: K_i=15µM) and TRPV1 (K_i=0.4µM; EC₅₀=33nM (human); EC₅₀=6.71nM (rat)). Also inhibits anandamide uptake (IC₅₀=9µM, K_i=14.1µM) and fatty acid amide hydrolase (FAAH) (IC₅₀=20µM).

Product Specific Literature References

NE-19550: a novel, orally active anti-inflammatory analgesic: L. Brand, et al.; Drugs Exp. Clin. Res. 13, 259 (1987) Abstract
The antinociceptive effect and pharmacokinetics of olvanil following oral and subcutaneous dosing in the mouse: W.K. Sietsema, et al.; Life Sci. 43, 1385 (1988) Abstract
Olvanil: more potent than capsaicin at stimulating the efferent function of sensory nerves: S.R. Hughes, et al.; Eur. J. Pharmacol. 219, 481 (1992) Abstract
Interactions between synthetic vanilloids and the endogenous cannabinoid system: V. Di Marzo, et al.; FEBS Lett. 436, 449 (1998) Abstract
Anandamide transport inhibition by the vanilloid agonist olvanil: M. Beltramo & D. Piomelli; Eur. J. Pharmacol. 364, 75 (1999) Abstract
Unsaturated long-chain N-acyl-vanillyl-amides (N-AVAMs): vanilloid receptor ligands that inhibit anandamide-facilitated transport and bind to CB1 cannabinoid receptors: D. Melck, et al.; BBRC 262, 275 (1999) Abstract
Vanilloid receptors on sensory nerves mediate the vasodilator action of anandamide: P.M. Zygmunt, et al.; Nature 400, 452 (1999) Abstract
Overlap between the ligand recognition properties of the anandamide transporter and the VR1 vanilloid receptor: inhibitors of anandamide uptake with negligible capsaicin-like activity: L. De Petrocellis, et al.; FEBS Lett. 483, 52 (2000) Abstract
Characterization using FLIPR of rat vanilloid receptor (rVR1) pharmacology: J.C. Jerman, et al.; Br. J. Pharmacol. 130, 916 (2000) Abstract
Evidence against the presence of an anandamide transporter: S.T. Glaser, et al.; PNAS 100, 4269 (2003) Abstract
Identification of species-specific determinants of the action of the antagonist capsazepine and the agonist PPAHV on TRPV1: E. Phillips, et al.; J. Biol. Chem. 279, 17165 (2004) Abstract
Effects of the vanilloid agonist olvanil and antagonist capsazepine on rat behaviors: J.W. Kasckow, et al.; Prog. Neuropsychopharmacol. Biol. Psychiatry 28, 291 (2004) Abstract

Further Categories Containing This Product:

Anandamide Uptake Inhibitors • Cannabinoid Receptor Agonists & Antagonists / Related Products • Fatty Acid Amide Hydrolase [FAAH] / Related Products

ALX-340-042

Revised 07-Jan-06

Arvanil
SYNONYMS	N-[(4-Hydroxy-3-methoxyphenyl)methyl]-5Z,8Z,11Z,14Z-eicosatetraenamide
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Cannabinoid Receptor Agonists & Antagonists / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-340-042-M005		5 mg	25.00 USD

Product Specification

FORMULA:	C₂₈H₄₁NO₃
MW:	439.6
CAS NUMBER:	128007-31-8
PURITY:	≥98%
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. Keep cool and dry.

Product Description

"Hybrid" activator of CB₁ receptor (CB₁: K_i=0.5µM; CB₂: K_i=>15µM) and TRPV1 (K_i=0.3µM). Also inhibits anandamide uptake (IC₅₀=3.6µM) and fatty acid amide hydrolase (FAAH) (IC₅₀=3µM). Analgesic, vasodilatory and anti-inflammatory in vivo. Apoptosis inducer.

Product Specific Literature References

Unsaturated long-chain N-acyl-vanillyl-amides (N-AVAMs): vanilloid receptor ligands that inhibit anandamide-facilitated transport and bind to CB1 cannabinoid receptors: D. Melck, et al.; BBRC 262, 275 (1999) Abstract
Neurobehavioral activity in mice of N-vanillyl-arachidonyl-amide: V. Di Marzo, et al.; Eur. J. Pharmacol. 406, 363 (2000) Abstract
A structure/activity relationship study on arvanil, an endocannabinoid and vanilloid hybrid: V. Di Marzo, et al.; J. Pharmacol. Exp. Ther. 300, 984 (2002) Abstract
The CB1/VR1 agonist arvanil induces apoptosis through an FADD/caspase-8-dependent pathway: R. Sancho, et al.; Br. J. Pharmacol. 140, 1035 (2003) Abstract
Evidence against the presence of an anandamide transporter: S.T. Glaser, et al.; PNAS 100, 4269 (2003) Abstract
Arvanil, a hybrid endocannabinoid and vanilloid compound, behaves as an antihyperkinetic agent in a rat model of Huntington's disease: E. de Lago, et al.; Brain Res. 1050, 210 (2005) Abstract
Arvanil inhibits T lymphocyte activation and ameliorates autoimmune encephalomyelitis: A.M. Malfitano, et al.; J. Neuroimmunol. 171, 110 (2006) Abstract

Further Categories Containing This Product:

Anandamide Uptake Inhibitors • Apoptosis Inducers & Inhibitors Other Products • TRPV1 Agonists and Antagonists / Related Products • Analgesic / Anti-nociceptive Agents / Related Products • Anti-inflammatory Agents Other Products • Fatty Acid Amide Hydrolase [FAAH] / Related Products

ALX-340-055

Revised 21-Jul-08

N-Arachidonoyl glycine
SYNONYMS	NAGly N-[1-Oxo-5Z,8Z,11Z,14Z-Eicosatetraenyl]-glycine
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Anandamide & Anandamide Analogs

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-340-055-M005		5 mg	55.00 USD

Product Specification

FORMULA:	C₂₂H₃₅NO₃
MW:	361.5
CAS NUMBER:	179113-91-8
CONCENTRATION:	50mg/ml
PURITY:	≥98%
FORMULATION:	Liquid. Solution in ethanol.
SOLUBILITY:	15mg/ml soluble in DMSO or dimethyl formamide; 2mg/ml soluble in PBS (pH 7.2).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 2 years after receipt when stored at -20°C. We do not recommend storing aqueous solutions for more than one day.

Product Description

Endogenous anandamide-like compound. Lacks affinity for CB₁ receptors (K_i>10µM), TRPV1 (EC₅₀>10µM) and anandamide uptake (IC₅₀>50µM), but inhibits fatty acid amide hydrolase