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ALX-350-270

Revised 08-Feb-08

α-Amanitin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Natural Products - DNA Regulation / Transcription

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-270-M001		1 mg	140.00 USD

Product Specification

FORMULA:	C₃₉H₅₄N₁₀O₁₄S
MW:	919.0
CAS NUMBER:	23109-05-9
MERCK INDEX:	14: 373
RTECS:	BD6195000
SOURCE/HOST:	Isolated from Amanita phalloides mushrooms.
PURITY:	≥90%
APPEARANCE:	White to light yellow powder.
SOLUBILITY:	Soluble in 100% ethanol (5mg/ml), DMSO, dimethyl formamide, methanol or water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Dilute with buffer immediately prior to use. Product is destroyed by concentrated acid or base.
HANDLING:	Protect from light.
HAZARD:	VERY TOXIC.

Product Description

Inhibits eukaryotic RNA-polymerase II with high efficiency, but not eukaryotic RNA-polymerase I, or bacterial or viral RNA-polymerases. Inhibits mammalian protein synthesis.

Product Specific Literature References

Effect of alpha-amanitin on ribonucleic acid synthesis and on ribonucleic acid polymerase in mouse liver: F. Stirpe & L. Fiume; Biochem. J. 103, 67P (1967) Abstract
Specific inhibition of nuclear RNA polymerase II by alpha-amanitin: T.J. Lindell, et al.; Science 170, 447 (1970) Abstract
Fifty years of amanitin: T. Wieland & H. Faulstich; Experientia 47, 1189 (1991), (Review) Abstract
Alpha-amanitin blocks translocation by human RNA polymerase II: X.Q. Gong, et al.; J. Biol. Chem. 279, 27422 (2004) Abstract

Further Categories Containing This Product:

Amanitins & Phalloidins / Related Products • RNA Polymerases / Related Products • Protein Synthesis Other Products

ALX-350-271

Revised 08-Oct-07

β-Amanitin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Natural Products - DNA Regulation / Transcription

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-271-M001		1 mg	170.00 USD

Product Specification

FORMULA:	C₃₉H₅₃N₉O₁₅S
MW:	920.0
CAS NUMBER:	21150-22-1
MERCK INDEX:	14: 373
SOURCE/HOST:	Isolated from Amanita phalloides mushrooms.
PURITY:	≥90%
FORMULATION:	White to off-white powder.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide, methanol or water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Dilute with buffer immediately prior to use. Product is destroyed by concentrated acid or base.
HANDLING:	Protect from light.
HAZARD:	VERY TOXIC.

Product Description

Biological activity like α-amanitin (Prod. No. ALX-350-270), but contains a carboxy group which is useful for coupling reactions.

Further Categories Containing This Product:

Amanitins & Phalloidins / Related Products • RNA Polymerases / Related Products • Protein Synthesis Other Products

ALX-350-272

Revised 17-Dec-07

γ-Amanitin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Natural Products - DNA Regulation / Transcription

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-272-M001		1 mg	170.00 USD

Product Specification

FORMULA:	C₃₉H₅₄N₁₀O₁₃S
MW:	903.0
CAS NUMBER:	13567-11-8; 21150-23-2
MERCK INDEX:	14: 373
SOURCE/HOST:	Isolated from Amanita phalloides mushrooms.
PURITY:	≥90%
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide or methanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Dilute with buffer immediately prior to use. Product is destroyed by concentrated acid or base.
HANDLING:	Protect from light.
HAZARD:	VERY TOXIC.

Product Description

Biological activity like α-amanitin (Prod. No. ALX-350-270).

Further Categories Containing This Product:

Amanitins & Phalloidins / Related Products • RNA Polymerases / Related Products • Protein Synthesis Other Products

ALX-380-071

Revised 18-Nov-08

Rifampicin
SYNONYMS	Rifampin 3-(4-Methylpiperazinyl-iminomethyl)rifamycin SV
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antibiotics for Cell Cycle Research

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-380-071-M100		100 mg	18.00 USD

Product Specification

FORMULA:	C₄₃H₅₈N₄O₁₂
MW:	823.0
CAS NUMBER:	13292-46-1
MERCK INDEX:	14: 8216
RTECS:	VJ7000000
SOURCE/HOST:	Semisynthetic.
PURITY:	≥95%
APPEARANCE:	Orange to brown powder.
SOLUBILITY:	Soluble in DMSO (25mg/ml) or methanol (25mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HAZARD:	HARMFUL.

Product Description

Antibacterial (tuberculostatic). Inhibits DNA-dependent RNA polymerase. Potent inducer of hepatic cytochrome P450 enzymes.

Product Specific Literature References

Rifampicin: a new orally active rifamycin: N. Maggi, et al.; Chemotherapy 11, 285 (1966) Abstract
Rifampicin sensitivity of the components of DNA-dependent RNA polymerase: E. di Mauro, et al.; Nature 222, 533 (1969) Abstract
Chemical and biological properties of rifampicin: S. Furesz; Antibiot. Chemother. 16, 316 (1970) Abstract
On the mechanism of rifampicin inhibition of RNA synthesis: W.R. McClure and C.L. Cech; J. Biol. Chem. 253, 8949 (1978) Abstract
Pharmacokinetic drug interactions with rifampicin: K. Venkatesan; Clin. Pharmacokinet. 22, 47 (1992), Review Abstract
Pharmacokinetic interactions with rifampicin : clinical relevance: M. Niemi, et al.; Clin. Pharmacokinet. 42, 819 (2003), Review Abstract
Roles of rifampicin in drug-drug interactions: underlying molecular mechanisms involving the nuclear pregnane X receptor: J. Chen and K. Raymond; Ann. Clin. Microbiol. Antimicrob. 50, 3 (2006), Review Abstract

Further Categories Containing This Product:

Other Antibiotics - DNA Regulation / Transcription • Cytochrome P450 [CYP450] / Related Products • RNA Polymerases / Related Products

ALX-380-092

Revised 03-Jul-08

Radicicol
SYNONYMS	Monorden 1aS-(1aR,2Z,4E,14,15aR*)]-8-Chloro-1a,14,15,15a-tetrahydro-9,11-dihydroxy-14-methyl-6H-oxireno[e][2]benzoxacyclotetradecin-6,12(7H)-dione
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antibiotics - Antifungal

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-380-092-M001		1 mg	75.00 USD
ALX-380-092-M005		5 mg	295.00 USD

Product Specification

FORMULA:	C₁₈H₁₇ClO₆
MW:	364.8
CAS NUMBER:	12772-57-5
MERCK INDEX:	14: 6253
RTECS:	RR1105000
SOURCE/HOST:	Isolated from Humicola fuscoatra.
PURITY:	≥98% (HPLC)
APPEARANCE:	White to light yellow powder.
SOLUBILITY:	Soluble in DMSO, methanol or acetone.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.
HAZARD:	MAY BE CARCINOGENIC. HARMFUL.

Product Description

Antifungal macrocyclic lactone antibiotic with antimalarial activity. Potent inhibitor of HSP90. Binds more strongly to HSP90 (nanomolar affinity) than to Grp94. Also binds to yeast HSP90, E. coli HtpG and TRAP-1. Non-competitive inhibitor of ATP citrate lyase. Anti-angiogenic. Specifically inhibits the interaction between HIF-1α/Arnt heterodimer and the hypoxia-responsive element (HRE), reducing VEGF expression. Protein tyrosine kinase inhibitor, e.g. p60^v-src(IC₅₀=0.27nM). Inhibitor of cyclooxygenase-2 (COX-2) expression (IC₅₀=27nM) without affecting COX-1 expression in LPS-stimulated macrophages. Induces the differentiation of HL-60 cells into macrophages, blocking cell cycle at G1 and G2. Suppressor of NIH 3T3 cell transformation by diverse oncogenes such as mos, ras and src in part by blocking the key signal transduction intermediates such as MAP kinase and GAP-associated p62. Inhibitor of AP-1-, NF-κB- and serum response factor (SRF)-mediated transcription (e.g. expression of iNOS). Represses the transcriptional function of the estrogen receptor. Inhibits archeal growth and DNA topoisomerase VI (a Topo IIB family topoisomerase). Blocks replication of negative-strand RNA viruses.

Product Specific Literature References

Potent and specific inhibition of p60v-src protein kinase both in vivo and in vitro by radicicol: H.J. Kwon, et al.; Cancer Res. 52, 6926 (1992) Abstract
Radicicol, a microbial cell differentiation modulator, inhibits in vivo angiogenesis: T. Oikawa, et al.; Eur. J. Pharmacol. 241, 221 (1993) Abstract
Radicicol, a protein tyrosine kinase inhibitor, suppresses the expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide and in experimental glomerulonephritis: P. Chanmugam, et al.; J. Biol. Chem. 270, 5418 (1995) Abstract; Full Text
Suppression of RAS and MOS transformation by radicicol: J.F. Zhao, et al.; Oncogene 11, 161 (1995) Abstract
Induction of differentiation of HL-60 cells by the anti-fungal antibiotic, radicicol: Y. Shimada, et al.; J. Antibiot. 48, 824 (1995) Abstract
Radicicol inhibits tyrosine phosphorylation of the mitotic Src substrate Sam68 and retards subsequent exit from mitosis of Src-transformed cells: I. Pillay, et al.; Cell Growth Differ. 7, 1487 (1996) Abstract
Radicicol leads to selective depletion of Raf kinase and disrupts K-Ras-activated aberrant signaling pathway: S. Soga, et al.; J. Biol. Chem. 273, 822 (1998) Abstract; Full Text
Antimalarial activity of radicicol, heptelidic acid and other fungal metabolites: Y. Tanaka, et al.; J. Antibiot. 51, 153 (1998) Abstract
Targeting of the protein chaperone, HSP90, by the transformation suppressing agent, radicicol: S.V. Sharma, et al.; Oncogene 16, 2369 (1998) Abstract
Structural basis for inhibition of the Hsp90 molecular chaperone by the antitumor antibiotics radicicol and geldanamycin: S.M. Roe, et al.; J. Med. Chem. 42, 260 (1999) Abstract
T.W. Schulte, et al.; Mol. Endocrinol. 13, 1435 (1999) Abstract; Full Text
Radicicol suppresses expression of inducible nitric-oxide synthase by blocking p38 kinase and nuclear factor-kappaB/Rel in lipopolysaccharide-stimulated macrophages: Y.J. Jeon, et al.; J. Pharmacol. Exp. Ther. 294, 548 (2000) Abstract; Full Text
Radicicol binds and inhibits mammalian ATP citrate lyase: S.W. Ki, et al.; J. Biol. Chem. 275, 39231 (2000) Abstract; Full Text
Radicicol suppresses transformation and restores tropomyosin-2 expression in both ras- and MEK-transformed cells without inhibiting the Raf/MEK/ERK signaling cascade: P.N. Kim, et al.; Cell Growth Differ. 12, 543 (2001) Abstract; Full Text
Radicicol-sensitive peptide binding to the N-terminal portion of GRP94: S. Vogen, et al.; J. Biol. Chem. 277, 40742 (2002) Abstract; Full Text
Reduction of hypoxia-induced transcription through the repression of hypoxia-inducible factor-1alpha/aryl hydrocarbon receptor nuclear translocator DNA binding by the 90-kDa heat-shock protein inhibitor radicicol: E. Hur, et al.; Mol. Pharmacol. 62, 975 (2002) Abstract; Full Text
Radicicol represses the transcriptional function of the estrogen receptor by suppressing the stabilization of the receptor by heat shock protein 90: M.O. Lee, et al.; Mol. Cell Endocrinol. 188, 47 (2002) Abstract
Development of radicicol analogues: S. Soga, et al.; Curr. Cancer Drug Targets 3, 359 (2003) Abstract
Heat shock protein 90 (Hsp90) chaperone complex inhibitor, radicicol, potentiated radiation-induced cell killing in a hormone-sensitive prostate cancer cell line through degradation of the androgen receptor: K. Harashima, et al.; Int. J. Radiat. Biol. 81, 63 (2005) Abstract
Inhibition of archaeal growth and DNA topoisomerase VI activities by the Hsp90 inhibitor radicicol: D. Gadelle, et al.; Nucleic Acid Res. 33, 2310 (2005) Abstract
Geldanamycin, radicicol, and chimeric inhibitors of the Hsp90 N-terminal ATP binding site: M.K. Hadden, et al.; Curr. Top. Med. Chem. 6, 1173 (2006), Review Abstract
The topoisomerase II-Hsp90 complex: a new chemotherapeutic target?: C.R. Barker, et al.; Int. J. Cancer 118, 2685 (2006) Abstract
Structural basis for topoisomerase VI inhibition by the anti-Hsp90 drug radicicol: K.D. Corbett & J.M. Berger; Nucleic Acids Res. 34, 4269 (2006) Abstract
Chemistry and biology of resorcylic acid lactones: N. Winssinger & S. Barluenga; Chem. Commun. (Camb.) 22 (2007) Abstract
Antiviral activity and RNA polymerase degradation following Hsp90 inhibition in a range of negative strand viruses: J.H. Connor, et al.; Virology 362, 109 (2007) Abstract

Further Categories Containing This Product:

Antibiotics - Protein Kinase Inhibitors • Cell Cycle Blockers & Inhibitors / Related Products • Estrogen & Estrogen Receptors / Related Products • Antibiotics - Nitric Oxide Pathway Modulators • Antitumor Agents (Hormone-related) • Tyrosine Kinase Inhibitors • NF-kB Pathway Inhibitors • Malaria / Related Products • Antibiotics - Antimalarial • Antibiotics - NF-kB Inhibitors • Antibiotics for Angiogenesis Research • Antibiotics for Cell Cycle Research • HSP90 / HtpG / Related Products • Antibiotics - Topoisomerase Inhibitors • RNA Polymerases / Related Products

ALX-380-111

Revised 16-Jun-08

Resistomycin
SYNONYMS	Geliomycin Heliomycin Itamycin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Antibiotics - DNA Regulation / Transcription

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-380-111-M001		1 mg	70.00 USD

Product Specification

FORMULA:	C₂₂H₁₆O₆
MW:	376.4
CAS NUMBER:	20004-62-0
RTECS:	DJ6350000
SOURCE/HOST:	Isolated from Streptomyces sp.
PURITY:	≥98% (HPLC)
APPEARANCE:	Yellow to orange solid.
SOLUBILITY:	Soluble in DMSO or methanol.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions are stable for at least 3 months when stored at -20°C.
HAZARD:	MAY BE CARCINOGENIC. HARMFUL.

Product Description

Antibacterial against Gram-positive and mycobacteria. Inhibits RNA polymerase. Induces apoptosis.

Product Specific Literature References

Effects of the antibiotic resistomycin on the synthesis of macromolecules: I. Haupt, et al.; Z. Allg. Mikrobiol. 156, 411 (1975) Abstract
3-Alkanoyl-5-hydroxymethyl tetronic acid homologues and resistomycin: new inhibitors of HIV-1 protease. I. Fermentation, isolation and biological activity: B.E. Roggo, et al.; J. Antibiot. (Tokyo) 47, 136 (1994) Abstract
Effects of Polyphenolic Anthrone Derivatives,Resistomycin and Hypericin, on Apoptosis in Human Megakaryoblastic Leukemia CMK-7 Cell Line: Y. Shiono et al.; Z. Naturforsch. 57c, 923 (2002) Full Text

Further Categories Containing This Product:

Antibiotics - Apoptosis Inducers & Inhibitors • RNA Polymerases / Related Products

ALX-380-200

Revised 31-Jul-08

Thiolutin
SYNONYMS	6-Acetamido-4-methyl-1,2-dithiolo(4,3-b)pyrrol-5(4H)-one Farcinicine Acetopyrrothine
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antibiotics - Antifungal

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-380-200-M001		1 mg	60.00 USD
ALX-380-200-M005		5 mg	240.00 USD

Product Specification

FORMULA:	C₈H₈N₂O₂S₂
MW:	228.3
CAS NUMBER:	87-11-6
MERCK INDEX:	14: 9342
RTECS:	JP1355000
SOURCE/HOST:	Isolated from Streptomyces luteosporeus.
PURITY:	≥98% (HPLC)
APPEARANCE:	Yellow powder.
SOLUBILITY:	Soluble in DMSO (1mg/ml); very slightly soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HAZARD:	VERY TOXIC.

Product Description

Sulfur-containing antibiotic. Potent inhibitor of bacterial and yeast RNA polymerases. Inhibits mRNA chain elongation. Inhibits adhesion of human umbilical vein endothelial cells (HUVECs) to vitronectin (IC₅₀=0.83µM) and thus suppresses tumor cell-induced angiogenesis in vivo. Inhibits microbiological growth in beer. Active against soil borne pathogens.

Product Specific Literature References

W.D. Celmer, et al.; JACS 74, 6304 (1952)
W.D. Celmer & I.A. Solomons; JACS 77, 2861 (1955)
Inhibition of yeast ribonucleic acid polymerases by thiolutin: D.J. Tipper; J. Bacteriol. 116, 245 (1973) Abstract; Full Text
Inhibition of messenger ribonucleic acid synthesis in Escherichia coli by thiolutin: G.G. Khachatourians and D.J. Tipper; J. Bacteriol. 119, 795 (1974) Abstract; Full Text
In vivo effect of thiolutin on cell growth and macromolecular synthesis in Escherichia coli: G.G. Khachatourians and D.J. Tipper; Antimicrob. Agents Chemother. 6, 304 (1974) Abstract; Full Text
Cell wall synthesis regulation in Saccharomyces cerevisiae. Effect of RNA and protein inhibition: M.V. Elorza, et al.; Biochim. Biophys. Acta 454, 263 (1976) Abstract
P.R. Deb & B.K. Dutta; Curr. Sci. 53, 659 (1984)
The yeast heat shock response is induced by conversion of cells to spheroplasts and by potent transcriptional inhibitors: C.C. Adams and D.S. Gross; J. Bacteriol. 173, 7429 (1991) Abstract; Full Text
Thiolutin, an inhibitor of HUVEC adhesion to vitronectin, reduces paxillin in HUVECs and suppresses tumor cell-induced angiogenesis: K. Minamiguchi, et al.; Int. J. Cancer 93, 307 (2001) Abstract
Genome-wide analysis of mRNA stability using transcription inhibitors and microarrays reveals posttranscriptional control of ribosome biogenesis factors: J. Grigull, et al.; Mol. Cell. Biol. 24, 5534 (2004) Abstract; Full Text
Determination of mRNA half-lives in Candida albicans using thiolutin as a transcription inhibitor: B.W. Kebaara, et al.; Genome 49, 894 (2006) Abstract

FORMULA:	C₉H₁₀N₂O₂S₂
MW:	242.3
CAS NUMBER:	574-95-8
SOURCE/HOST:	Isolated from Streptomyces sp. MST-AS4782.
PURITY:	≥98% (HPLC)
APPEARANCE:	Yellow to orange solid.
SOLUBILITY:	Soluble in DMSO or dimethyl formamide; partially soluble in methanol or 100% ethanol; poorly soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C