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Ubiquitin Ligases [E3]
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ALX-201-215 Revised 02-Apr-08
CHIP (human) (recombinant)
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SYNONYMS C-terminus of HSC70 Interacting Protein
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Co-Chaperones & HSP-interacting Proteins / Related Products
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ALX-201-215-C025   25 µg 160.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥99%
FORMULATION: Liquid. In 50mM HEPES/KOH, pH 7.0, containing 50mM potassium chloride, 5mM DTT and 10% (v/v) glycerol.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Dimerization of the human E3 ligase CHIP via a coiled-coil domain is essential for its activity: R. Nikolay, et al.; J. Biol. Chem. 279, 2673 (2004) Abstract; Full Text
Further Categories Containing This Product:
Chaperone-mediated Autophagy [CMA] • Recombinant Proteins / Fusion Proteins • Ubiquitin Ligases [E3]
 
 
ALX-430-128 Revised 18-Nov-08
Nutlin-3
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SYNONYMS (±)-4-[4,5-bis-(4-Chlorophenyl)-2-(2-isopropoxy-4-methoxyphenyl)-4,5-dihydroimidazole-1-carbonyl]-piperazin-2-one
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Other Products
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ALX-430-128-M001   1 mg 35.00 USD Add To Cart
ALX-430-128-M005   5 mg 160.00 USD Add To Cart
ALX-430-128-M025   25 mg 560.00 USD Add To Cart
Product Specification
FORMULA: C30H30Cl2N4O4
MW: 581.5
CAS NUMBER: 548472-68-0
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white crystalline solid.
SOLUBILITY: Soluble in methanol, 100% ethanol, DMSO, dimethyl formamide or acetonitrile; sparingly soluble in a 1:10 solution of ethanol:PBS, pH 7.2.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least one year after receipt when stored at -20°C. We do not recommend storing the aqueous solution for more than one day.
HAZARD: IRRITANT.

Product Description
Inhibitor of p53/MDM2 interaction (IC50=0.09µM).
Product Specific Literature References
In vivo activation of the p53 pathway by small-molecule antagonists of MDM2: L.T. Vassilev, et al.; Science 303, 844 (2004) Abstract
Reactivation of the p53 pathway as a treatment modality for KSHV-induced lymphomas: G. Sarek, et al.; J. Clin. Invest. 117, 1019 (2007) Abstract; Full Text
General Literature References
Drug discovery and p53: D.P. Lane, et al.; Drug Discov. Today 8, 347 (2003) Abstract
Further Categories Containing This Product:
Ubiquitin Ligases [E3]
 
 
ALX-210-394 Revised 30-Oct-06
Polyclonal Antibody to Asb-2 (human)
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SYNONYMS anti-Ankyrin Repeat and SOCS Box Containing Protein 2 (human) PAb
PRODUCT LINE Ubiquitin & Proteasome
PRODUCT CATEGORY Ubiquitin Ligases [E3]
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ALX-210-394-R200   200 µl 267.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Protein G purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptides containing aa 75-93 and 348-368 of human Asb-2 (ankyrin repeat and SOCS box containing protein 2).
SPECIFICITY: Recognizes human Asb-2.
APPLICATION: Western Blot (1:500-1:1'000).
Other applications not tested.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
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General Information
The ankyrin repeat and SOCS box-containing (Asb) protein family is comprised of members which each contain “SOCS boxes” but differ from other families of protein in the motifs they contain upstream of the SOCS box. SOCS boxes are C-terminal regions of homology found in the suppressor of cytokine signaling family of proteins. The box region is thought to be the point of interaction between SOCS proteins and E3 ubiquitin ligases. Asb-2 has been found to induce growth inhibition and chromatin condensation in myeloid leukemia cells.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-011 Revised 22-Apr-08
Polyclonal Antibody to BAG-1 (Bur 1680)
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SYNONYMS anti-Bcl-2 Associated Athanogene-1 PAb (Bur 1680)
anti-BAG Family Molecular Chaperone Regulator 1 (Bur 1680) PAb
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bcl-2 Family Interacting Proteins / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-011-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Recombinant mouse BAG-1 (Bcl-2 associated athanogene-1) (aa 8-219).
SPECIFICITY: Recognizes mouse and rat BAG-1.
APPLICATION: Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections (1:5'000))
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -80°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Bag1 is a regulator and marker of neuronal differentiation: P. Kermer, et al.; Cell Death Differ. 9, 405 (2002) Abstract
General Information
BAG (Bcl-2-associated athanogene) family proteins contain a BAG domain and interact with a variety of partners including heat shock proteins, steroid hormone receptors, Bcl-2 and Raf-1. BAG-1 and BAG-3 participate in a wide variety of cellular processes including signalling, transcription, stress response, and apoptosis. BAG-1 functions as a nucleotide exchange factor of mammalian cytosolic HSC70, thereby triggering substrate unloading from the chaperone. In addition BAG-1 associates with the proteasome, playing a role in coordinating chaperone and degradation pathways. Aberrant BAG-1 expression has been observed in certain human cancers.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
BAG-1--a nucleotide exchange factor of Hsc70 with multiple cellular functions: S. Alberti, et al.; Cell Stress Chaperones 8, 225 (2003) Abstract; Full Text
BAG-1: a multi-functional pro-survival molecule: P.A. Townsend, et al.; Int. J. Biochem. Cell. Biol. 37, 251 (2005) Abstract
Further Categories Containing This Product:
Co-Chaperones & HSP-interacting Proteins / Related Products • Chaperone-mediated Autophagy [CMA] • Polyclonal Antibodies • Ubiquitin Ligases [E3]
 
 
ALX-210-009 Revised 22-Apr-08
Polyclonal Antibody to BAG-1 (mouse) (CT) (Bur 1702)
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SYNONYMS anti-Bcl-2 Associated Athanogene-1 (mouse) (CT) PAb (Bur 1702)
anti-BAG Family Molecular Chaperone Regulator 1 (mouse) (CT) (Bur 1702) PAb
anti-RAP46 (mouse) (CT) (Bur 1702) PAb
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bcl-2 Family Interacting Proteins / Related Products
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ALX-210-009-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 204-219 (C204QETERLQSTNLALAE219) of mouse BAG-1 (Bcl-2 associated athanogene-1).
SPECIFICITY: Recognizes mouse BAG-1.
APPLICATION: Immunohistochemistry (paraffin sections (1:2'500))
Immunoprecipitation (transfected cells)
Western Blot (1:500-1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -80°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity: S. Takayama, et al.; Cell 80, 279 (1995) Abstract
Interleukin-2 (IL-2) upregulates BAG-1 gene expression through serine-rich region within IL-2 receptor beta c chain: M. Adachi, et al.; Blood 88, 4118 (1996) Abstract; Full Text
Bcl-2 interacting protein, BAG-1, binds to and activates the kinase Raf-1: H.G. Wang, et al.; PNAS 93, 7063 (1996) Abstract; Full Text
Role of Bag-1 in the survival and proliferation of the cytokine-dependent lymphocyte lines, Ba/F3 and Nb2: C.V. Clevenger, et al.; Mol. Endocrinol. 11, 608 (1997) Abstract; Full Text
Bag1 is a regulator and marker of neuronal differentiation: P. Kermer, et al.; Cell Death Differ. 9, 405 (2002) Abstract
General Information
BAG (Bcl-2-associated athanogene) family proteins contain a BAG domain and interact with a variety of partners including heat shock proteins, steroid hormone receptors, Bcl-2 and Raf-1. BAG-1 and BAG-3 participate in a wide variety of cellular processes including signalling, transcription, stress response, and apoptosis. BAG-1 functions as a nucleotide exchange factor of mammalian cytosolic HSC70, thereby triggering substrate unloading from the chaperone. In addition BAG-1 associates with the proteasome, playing a role in coordinating chaperone and degradation pathways. Aberrant BAG-1 expression has been observed in certain human cancers.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
BAG-1--a nucleotide exchange factor of Hsc70 with multiple cellular functions: S. Alberti, et al.; Cell Stress Chaperones 8, 225 (2003) Abstract; Full Text
BAG-1: a multi-functional pro-survival molecule: P.A. Townsend, et al.; Int. J. Biochem. Cell. Biol. 37, 251 (2005) Abstract
Further Categories Containing This Product:
Co-Chaperones & HSP-interacting Proteins / Related Products • Chaperone-mediated Autophagy [CMA] • Polyclonal Antibodies • Ubiquitin Ligases [E3]
 
 
ALX-210-010 Revised 22-Apr-08
Polyclonal Antibody to BAG-1 (NT) (Bur 1735)
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SYNONYMS anti-Bcl-2 Associated Athanogene-1 (NT) PAb (Bur 1735)
anti-BAG-family Molecular Chaperone Regulator 1 (NT) (Bur 1735) PAb
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bcl-2 Family Interacting Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-010-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 26-46 (N26ERYDLLVTPQQGNSEPVVQD46) of mouse BAG-1 (Bcl-2 associated athanogene-1).
SPECIFICITY: Recognizes mouse and rat BAG-1.
APPLICATION: Immunocytochemistry (1:1'000)
Immunohistochemistry (paraffin sections (1:3'500))
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -80°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Bag1 is a regulator and marker of neuronal differentiation: P. Kermer, et al.; Cell Death Differ. 9, 405 (2002) Abstract
General Information
BAG (Bcl-2-associated athanogene) family proteins contain a BAG domain and interact with a variety of partners including heat shock proteins, steroid hormone receptors, Bcl-2 and Raf-1. BAG-1 and BAG-3 participate in a wide variety of cellular processes including signalling, transcription, stress response, and apoptosis. BAG-1 functions as a nucleotide exchange factor of mammalian cytosolic HSC70, thereby triggering substrate unloading from the chaperone. In addition BAG-1 associates with the proteasome, playing a role in coordinating chaperone and degradation pathways. Aberrant BAG-1 expression has been observed in certain human cancers.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
BAG-1--a nucleotide exchange factor of Hsc70 with multiple cellular functions: S. Alberti, et al.; Cell Stress Chaperones 8, 225 (2003) Abstract; Full Text
BAG-1: a multi-functional pro-survival molecule: P.A. Townsend, et al.; Int. J. Biochem. Cell. Biol. 37, 251 (2005) Abstract
Further Categories Containing This Product:
Co-Chaperones & HSP-interacting Proteins / Related Products • Chaperone-mediated Autophagy [CMA] • Polyclonal Antibodies • Ubiquitin Ligases [E3]
 
 
ALX-210-883 Revised 02-Apr-07
Polyclonal Antibody to CHIP (human)
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SYNONYMS anti-C-terminus of HSC70 interacting Protein (human) PAb
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Co-Chaperones & HSP-interacting Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-883-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 218-232 (V218DEKRKKRDIPDYLC232) of human CHIP (C-terminus of HSC70 interacting protein).
SPECIFICITY: Recognizes C-terminal human CHIP. Detects bands of ~35kDa (representing CHIP) and ~85kDa (non-specific) by Western blot.
APPLICATION: Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-153-056.
Related Products
Further Categories Containing This Product:
Chaperone-mediated Autophagy [CMA] • Polyclonal Antibodies • Ubiquitin Ligases [E3]
 
 
ALX-210-260 Revised 13-Jan-05
Polyclonal Antibody to E6-AP (human)
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SYNONYMS anti-Angelmann Syndrome-associated Protein (human) PAb
PRODUCT LINE Ubiquitin & Proteasome
PRODUCT CATEGORY Ubiquitin Ligases [E3]
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-260-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Antiserum diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Recombinant full length human E6-AP (Angelmann syndrome-associated protein).
SPECIFICITY: Recognizes human E6-AP. Detects a band of ~100kDa by Western blot.
APPLICATION: Immunoprecipitation
Western Blot (1:2'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Description
Angelman Syndrome (AS) is a neurological disorder characterized by severe mental retardation, seizures and speech impairment, which results from the lack of a functional maternal copy of the E6-AP gene. E6-AP, or Angelman Syndrome-Associated protein, has been shown to interact with the progesterone receptor as a coactivator, enhancing the transcriptional activity of this steroid receptor. Interestingly, E6-AP (UBE3A) is also a member of the E3 ubiquitin-protein ligase family which plays a role in defining the substrate specificity of the ubiquitin-proteasome degradation system. For example, E6-AP can interact directly with the growth and tumor-suppressive protein, p53 or indirectly as part of a human papillomavirus type 16 or 18 E6 protein complex. This interaction promotes the degradation of p53 via the ubiquitin-proteosome pathway. These two functions of E6-AP, coactivation and degradation, are independent of one another. Furthermore, the coactivation function of E6-AP in AS patients remains intact and the ubiquitin-protease function of the protein was compromised. It is unclear, however, how this causes AS.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
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