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Transcription Factors / Related Products
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ALX-163-011 Revised 14-Jul-08
NBD Peptide /  NF-κB Blocker (cell permeable)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Pathway Inhibitors
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ALX-163-011-1   1 Vial 240.00 USD Add To Cart
Product Specification
SEQUENCE: GRKKRRQRRR-GG-TALDWSWLQTE-amide
FORMULA: C121H202N48O32
MW: 2841.2
QUANTITY: 50µl
CONCENTRATION: 10mM
PURITY: ≥95% (HPLC)
FORMULATION: Liquid. In sterile PBS.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Protect from moisture. Keep on ice at all times.
POSITIVE CONTROL: Prod. No. ALX-168-009/1
Product Description
For increased cell permeability the peptide was covalently linked to the 10 aa recognized by the TAT transporter.
Product Specific Literature References
Selective inhibition of NF-kappaB blocks osteoclastogenesis and prevents inflammatory bone destruction in vivo: E. Jimi, et al.; Nature Med. 10, 617 (2004) Abstract
Selective inhibition of NF-kappaB activation by a peptide that blocks the interaction of NEMO with the IkappaB kinase complex: M.J. May, et al.; Science 289, 1550 (2000) Abstract
Characterization of the Ikappa B-kinase NEMO binding domain: M.J. May, et al.; J. Biol. Chem. 277, 45992 (2002) Abstract
Related Products
Further Categories Containing This Product:
Peptides
 
 
ALX-167-024 Revised 17-May-06
SN50
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Pathway Inhibitors
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ALX-167-024-C500   500 µg 125.00 USD Add To Cart
Product Specification
SEQUENCE: H-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Val-Gln-Arg-Lys-Arg-Gln-Lys-Leu-Met-Pro-OH
FORMULA: C129H230N36O29S
MW: 2781.5
PEPTIDE CONTENT: 50-75%
PURITY: ≥96% (HPLC)
APPEARANCE: White to off-white solid.
FORMULATION: Counter Ion: Trifluoroacetate salt.
SOLUBILITY: Soluble in distilled water, 100% ethanol or ethanol:water 1:1.
RECONSTITUTION: Reconstitute in sterile distilled water.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Peptides in solution have limited stability.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Description
Inhibits translocation of the NF-κB active complex into the nucleus. In mouse endothelial LE-II cells induced with LPS, inhibition of NF-κB nuclear translocation was 88% at 50µg/ml (18µM). In human monocytic THP-1 cells induced by LPS or TNF-α there was 85% inhibition at 100µg/ml. For control peptide, see SN50M (Prod. No. ALX-167-025).
Product Specific Literature References
Inhibition of nuclear translocation of transcription factor NF-kappa B by a synthetic peptide containing a cell membrane-permeable motif and nuclear localization sequence: Y.Z. Lin, et al.; J. Biol. Chem. 270, 14255 (1995) Abstract; Full Text
p38 MAP kinase and MKK-1 co-operate in the generation of GM-CSF from LPS-stimulated human monocytes by an NF-kappa B-independent mechanism: K.K. Meja, et al.; Br. J. Pharmacol. 131, 1143 (2000) Abstract
Nuclear factor-kappaB activates dual inhibition sites in the regulation of tumor necrosis factor-alpha-induced neutrophil apoptosis: M. Niwa, et al.; Eur. J. Pharmacol 407, 211 (2000) Abstract
Activation of nuclear factor kappaB and induction of apoptosis by tumor necrosis factor-alpha in the mouse uterine epithelial WEG-1 cell line: S. Pampfer, et al.; Biol. Reprod. 63, 879 (2000) Abstract
Interruption of the NF-kappaB pathway by Bay 11-7082 promotes UCN-01-mediated mitochondrial dysfunction and apoptosis in human multiple myeloma cells: Y. Dai, et al.; Blood 103, 2761 (2004) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Nuclear localization sequence (NLS) aa 360-369 (AAVALLPAVLLALLAPVQRKRQKLMP) of the transcription factor NF-κB p50 linked to a peptide cell-permeablization sequence, the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF).
Further Categories Containing This Product:
Peptides • Nuclear Envelope & Nuclear Transport
 
 
ALX-167-025 Revised 17-May-06
SN50M Control Peptide
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Pathway Inhibitors
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ALX-167-025-C500   500 µg 125.00 USD Add To Cart
Product Specification
SEQUENCE: H-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Pro-Val-Gln-Arg-Asn-Gly-Gln-Lys-Leu-Met-Pro-OH
FORMULA: C123H215N33O30S
MW: 2668.4
PEPTIDE CONTENT: 70-90%
PURITY: ≥97% (HPLC)
APPEARANCE: White to off-white solid.
FORMULATION: Counter Ion: Trifluoroacetate salt.
SOLUBILITY: Soluble in water.
RECONSTITUTION: Reconstitute in sterile distilled water.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Peptides in solution have limited stability.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Description
Inactive control peptide for SN50 (Prod. No. ALX-167-024). In mouse endothelial LE-II cells, induced with LPS, 50µg/ml SN50M had no measurable effect on NF-κB translocation. In human monocytic THP-1 cells induced by LPS or TNF-α there was slight inhibition at 100µg/ml.
Product Specific Literature References
Inhibition of nuclear translocation of transcription factor NF-kappa B by a synthetic peptide containing a cell membrane-permeable motif and nuclear localization sequence: Y.Z. Lin, et al.; J. Biol. Chem. 270, 14255 (1995) Abstract; Full Text
Interruption of the NF-kappaB pathway by Bay 11-7082 promotes UCN-01-mediated mitochondrial dysfunction and apoptosis in human multiple myeloma cells: Y. Dai, et al.; Blood 103, 2761 (2004) Abstract
Further Categories Containing This Product:
Peptides • Nuclear Envelope & Nuclear Transport
 
 
ALX-201-024 Revised 24-Feb-05
HSF1 (human) (recombinant)
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SYNONYMS Heat Shock Factor 1 (human) (recombinant)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Stress & Heat Shock Proteins Other Products
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ALX-201-024-C150   150 µg 343.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli.
FORMULATION: Liquid. Sonicated E. coli extract at a total protein concentration of 6mg/ml in 25mM HEPES, pH 7.9, 12.5mM MgCl, 0.1mM EDTA, 10% glycerol, 1mM DTT, 0.1% NP-40 and 300mM KCl and protease inhibitors.
APPLICATION: Positive control in gel shift assays (25 tests) or Western blot (50 tests) with PAb to HSF1 (Prod. No. ALX-210-129). DNase I footprinting and in vitro transcription assays.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -20°C
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q00613: HSF 1 (human)
Further Categories Containing This Product:
Transcription Factors Other Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-056 Revised 21-Jul-08
Caspase-1 (active) (human) (recombinant)
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SYNONYMS Interleukin 1β Converting Enzyme (active) (human) (recombinant)
IL-1β Converting Enzyme (active) (human) (recombinant)
ICE (active) (human) (recombinant)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-056-U025   25 U 231.00 USD Add To Cart
ALX-201-056-U100   100 U 385.00 USD Add To Cart
Product Specification
EC: 3.4.22.36
SOURCE/HOST: Produced in E. coli.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY: ~20’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD-pNA (pNA: p-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION: Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
QUALITY CONTROL: Routinely tested for its ability to cleave the caspase-1 colorimetric substrate Ac-YVAD-pNA (Prod. No. ALX-260-026).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
Caspase-1 is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kDa) and small (10kDa) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P29466: Caspase-1 (human) (precursor)
AfCS Signalling Gateway link A000492: Caspase-1 (mouse)
Further Categories Containing This Product:
Inflammasome • Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-074 Revised 18-Sep-07
EGR-1 Protein (human) (recombinant)
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SYNONYMS Early Growth Response Protein 1 (human) (recombinant)
NGFI-A (human) (recombinant)
krox24 (human) (recombinant)
TIS-8 (human) (recombinant)
zif 268 (human) (recombinant)
pat 225 (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Egr Protein Family / Related Products
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ALX-201-074-1   1 Vial 365.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in Sf9 cells.
QUANTITY: 50µl, corresponding to >25 band forming units (BFU). Sufficient for performing at least 25 gel shift assays under standard conditions [2].
FORMULATION: Liquid. In 20mM HEPES, pH 7.9, containing 25% glycerol, 420mM sodium chloride, 0.2mM EDTA and 1.5mM magnesium chloride.
SPECIFICITY: Binds specifically to an oligonucleotide with the sequence GCG GGG GCG.
SPECIFIC ACTIVITY: >0.5BFU/µl. 1BFU is sufficient to generate a band shift with a labelled oligonucleotide.
APPLICATION: EMSA promoter characterization, in vitro transcription assays, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Description
The recombinant EGR-1 protein can be employed in the following fields of research: a) Promoter studies: Identification of EGR-1 binding promoter elements. b) Detection of EGR-1 interacting proteins. c) Signal transduction: Assay for the effect on the nuclear component EGR-1. d) Screening for EGR-1 activity in tumor cells and lines. e) Drug screening: Effect on EGR-1 generation and DNA binding.
Product Specific Literature References
A regulatory element in the human interleukin 2 gene promoter is a binding site for the zinc finger proteins Sp1 and EGR-1: C. Skerka, et al.; J. Biol. Chem. 270, 22500 (1995) Abstract; Full Text
The early growth response protein (EGR-1) regulates interleukin-2 transcription by synergistic interaction with the nuclear factor of activated T cells: E.L. Decker, et al.; J. Biol. Chem. 273, 26923 (1998) Abstract; Full Text
The human zinc finger protein EGR-4 acts as autoregulatory transcriptional repressor: P.F. Zipfel, et al.; Biochim. Biophys. Acta. 1354, 134 (1997) Abstract
Zinc finger transcription factors as molecular targets for nitric oxide-mediated immunosuppression: inhibition of IL-2 gene expression in murine lymphocytes: D. Berendji, et al.; Mol. Med. 5, 721 (1999) Abstract
Fibroblast growth factor-2 induction of platelet-derived growth factor-C chain transcription in vascular smooth muscle cells is ERK-dependent but not JNK-dependent and mediated by Egr-1: V.C. Midgley and L.M. Khachigian; J. Biol. Chem. 279, 40289 (2004) Abstract; Full Text
General Information
Early Growth Response- (EGR-) proteins represent a family of related transcription factors with almost identical DNA- binding zinc finger domains, but with different flanking regions. All four human EGR-proteins, EGR-1 to EGR-4 bind the EGR-consensus sequence GCG T/GGG GCG. However individual EGR-proteins show different binding to related sequences, such as the overlapping EGR-1/Sp1 binding site in the human IL-2 gene promoter. In functional assays the EGR-4 protein shows superior performance for Electrophoretic Mobility Shift Assay (EMSA) compared to proteins expressed in E.coli.
BACKGROUND/TECHNICAL INFORMATION For EMSA labeled oligonucleotides are required, that include the specific target sequence. Radioactive labeling of the oligonucleotide is performed by kinase reaction (T4 kinase) using either 32P-γ ATP or 33P-γ ATP as a source for radioactivity. Due to the lower radiation load we recommend the use of 33P-γ ATP, which results in specific, detectable bands. Formation of a specific protein: DNA complex results in a lower mobility of this complex compared to the mobility of the free DNA. The specificity of the EMSA can also be analyzed by competition experiments in which an excess of unlabeled specific oligonucleotide interferes with the formation of a specific band, while the same amount of unrelated or mutated oligonucleotide has no effect. DNA-Protein complexes are then separated from the free (unbound) oligonucleotides by native gel-electrophoresis.

Swiss-Prot link P18146: EGR-1 (human)
AfCS Signalling Gateway link A003269: EGR-1 (mouse)
General Literature References
A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization: V.P. Sukhatme, et al.; Cell 53, 37 (1988) Abstract
Identification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals: X.M. Cao, et al.; Mol. Cell. Biol. 10, 1931 (1990) Abstract
Early growth response protein 1 (Egr-1): prototype of a zinc-finger family of transcription factor: A. Gashler & V.P. Sukhatme; Prog. Nucleic Acid Res. Mol. Biol. 50, 191 (1995) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-075 Revised 02-May-06
EGR-3 Protein (human) (recombinant)
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SYNONYMS Early Growth Response Protein 3 (human) (recombinant)
Pilot (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Egr Protein Family / Related Products
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ALX-201-075-1   1 Vial 395.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in Sf9 cells.
QUANTITY: 75µl, corresponding to >25 band forming units (BFU). Sufficient for performing at least 25 gel shift assays under standard conditions.
FORMULATION: Liquid. In 20mM HEPES, pH 7.9, containing 25% glycerol, 420mM sodium chloride, 0.2mM EDTA and 1.5mM magnesium chloride.
SPECIFICITY: Binds specifically to an oligonucleotide with the sequence GCG GGG GCG.
SPECIFIC ACTIVITY: >0.33BFU/µl. 1BFU is sufficient to generate a band shift with a labelled oligonucleotide.
APPLICATION: EMSA promoter characterization, in vitro transcription assay, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as positive control).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Description
The recombinant EGR-3 protein can be employed in the following fields of research: a) Promoter studies: Identification of EGR-3 binding promoter elements. b) Detection of EGR-3 interacting proteins. c) Signal transduction: Assay for the effect on the nuclear component EGR-3. d) Screening for EGR-3 activity in tumor cells and lines. e) Drug screening: Effect on EGR-3 generation and DNA binding.
Product Specific Literature References
EGR3, a novel member of the Egr family of genes encoding immediate- early transcription factors: S. Patwardhan, et al.; Oncogene 6, 917 (1991) Abstract
Coordinate expression and distinct DNA-binding characteristics of the four EGR-zinc finger proteins in Jurkat T lymphocytes: C. Skerka, et al.; Immunobiology 198, 179 (1997) Abstract
The human zinc finger protein EGR-4 acts as autoregulatory transcriptional repressor: P.F. Zipfel, et al.; Biochim. Biophys. Acta 354, 134 (1997) Abstract
The early growth response protein (EGR-1) regulates interleukin-2 transcription by synergistic interaction with the nuclear factor of activated T cells: E.L. Decker, et al.; J. Biol. Chem. 273, 26923 (1998) Abstract; Full Text
Cyclosporin A-sensitive transcription factor Egr-3 regulates Fas ligand expression: P.R. Mittelstadt & J.D. Ashwell; Mol. Cell. Biol. 18, 3744 (1998) Abstract; Full Text
Role of Egr-2 in up-regulation of Fas ligand in normal T cells and aberrant double-negative lpr and gld T cells: P.R. Mittelstadt & J.D. Ashwell; J. Biol. Chem. 274, 3222 (1999) Abstract; Full Text
General Information
Early Growth Response- (EGR-) proteins represent a family of related transcription factors with almost identical DNA- binding zinc finger domains, but with different flanking regions. All four human EGR-proteins, EGR-1 to EGR-4 bind the EGR-consensus sequence GCG T/GGG GCG. However individual EGR-proteins show different binding to related sequences, such as the overlapping EGR-1/Sp1 binding site in the human IL-2 gene promoter. In functional assays the EGR-4 protein shows superior performance for Electrophoretic Mobility Shift Assay (EMSA) compared to proteins expressed in E.coli.
BACKGROUND/TECHNICAL INFORMATION For EMSA labeled oligonucleotides are required, that include the specific target sequence. Radioactive labeling of the oligonucleotide is performed by kinase reaction (T4 kinase) using either 32P-γ ATP or 33P-γ ATP as a source for radioactivity. Due to the lower radiation load we recommend the use of 33P-γ ATP, which results in specific, detectable bands. Formation of a specific protein: DNA complex results in a lower mobility of this complex compared to the mobility of the free DNA. The specificity of the EMSA can also be analyzed by competition experiments in which an excess of unlabeled specific oligonucleotide interferes with the formation of a specific band, while the same amount of unrelated or mutated oligonucleotide has no effect. DNA-Protein complexes are then separated from the free (unbound) oligonucleotides by native gel-electrophoresis.

Swiss-Prot link Q06889: EGR-3 (human)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-076 Revised 26-Jul-05
EGR-4 Protein (human) (recombinant)
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SYNONYMS Early Growth Response Protein 4 (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Egr Protein Family / Related Products
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ALX-201-076-1   1 Vial 395.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in Sf9 cells.
QUANTITY: 10µl. Sufficient for >10 Western blots or >50 gel shift assays under standard conditions.
FORMULATION: Liquid. In 20mM HEPES, pH 7.9, containing 25% glycerol, 420mM sodium chloride, 0.2mM EDTA and 1.5mM magnesium chloride.
BIOLOGICAL ACTIVITY: Binds specifically to an oligonucleotide with the sequence GCG GGG GCG.
APPLICATION: EMSA promoter characterization, in vitro transcription assay, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as positive control).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Description
The recombinant EGR-4 protein can be employed in the following fields of research: a) Promoter studies: Identification of EGR-4 binding promoter elements. b) Detection of EGR-4 interacting proteins. c) Signal transduction: Assay for the effect on the nuclear component EGR-4. d) Screening for EGR-4 activity in tumor cells and lines. e) Drug screening: Effect on EGR-4 generation and DNA binding.
Product Specific Literature References
The human zinc finger protein EGR-4 acts as autoregulatory transcriptional repressor: P.F. Zipfel, et al.; Biochim. Biophys. Acta 1354, 134 (1997) Abstract
The early growth response protein (EGR-1) regulates interleukin-2 transcription by synergistic interaction with the nuclear factor of activated T cells: E.L. Decker, et al.; J. Biol. Chem. 273, 26923 (1998) Abstract; Full Text
General Information
Early Growth Response- (EGR-) proteins represent a family of related transcription factors with almost identical DNA- binding zinc finger domains, but with different flanking regions. All four human EGR-proteins, EGR-1 to EGR-4 bind the EGR-consensus sequence GCG T/GGG GCG. However individual EGR-proteins show different binding to related sequences, such as the overlapping EGR-1/Sp1 binding site in the human IL-2 gene promoter. In functional assays the EGR-4 protein shows superior performance for Electrophoretic Mobility Shift Assay (EMSA) compared to proteins expressed in E.coli.
BACKGROUND/TECHNICAL INFORMATION

For EMSA labeled oligonucleotides are required, that include the specific target sequence. Radioactive labeling of the oligonucleotide is performed by kinase reaction (T4 kinase) using either 32P-ATP or 33P-ATP as a source for radioactivity. Due to the lower radiation load we recommend the use of 33P-ATP, which results in specific, detectable bands. Formation of a specific protein: DNA complex results in a lower mobility of this complex compared to the mobility of the free DNA. The specificity of the EMSA can also be analyzed by competition experiments in which an excess of unlabeled specific oligonucleotide interferes with the formation of a specific band, while the same amount of unrelated or mutated oligonucleotide has no effect. DNA-Protein complexes are then separated from the free (unbound) oligonucleotides by native gel-electrophoresis.
Swiss-Prot link Q05215: EGR-4 (human)
General Literature References
The early response gene NGFI-C encodes a zinc finger transcriptional activator and is a member of the GCGGGGGCG (GSG) element-binding protein family: S.D. Crosby, et al.; Mol. Cell. Biol. 11, 3835 (1991) Abstract
Clone pAT 133 identifies a gene that encodes another human member of a class of growth factor-induced genes with almost identical zinc-finger domains: H.J. Muller, et al.; PNAS 88, 10079 (1991) Abstract
Genomic organization, chromosomal localization and promoter function of the human zinc-finger gene pAT133: C. Holst, et al.; Hum. Mol. Genet. 2, 367 (1993) Abstract
Neural-specific expression, genomic structure, and chromosomal localization of the gene encoding the zinc-finger transcription factor NGFI-C: S.D. Crosby, et al.; PNAS 89, 4739 (1992) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-094 Revised 03-May-06
Caspase-5 (active) (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-094-U025   25 U 203.00 USD Add To Cart
ALX-201-094-U100   100 U 371.00 USD Add To Cart
Product Specification
EC: 3.4.22.-
SOURCE/HOST: Produced in E. coli.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY: ~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION: In combination with caspase-5 activity assays, the enzyme is useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase-5 activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING: SHIPPED ON DRY ICE