ALEXIS Biochemicals: Quick Apoptotic DNA Ladder Detection Kit

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ALX-850-043

Revised 27-Jul-05

ABSOLUTE-S™ Kit
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	DNA-based Apoptosis Detection

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-043-KI01		1 Kit	540.00 USD

Product Specification

QUANTITY:	50 assays.
KIT/SET CONTAINS:	Contains all the reagents necessary, including positive and negative control cells.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-20°C
POSITIVE CONTROL INCLUDED:	yes

Product Description

The kit utilizes the SBIP™ (strand break induced photolysis) methodology in which cells are irradiated with UV light to induce strand breaks. Does not require DNA denaturation and, therefore, is applicable in studies where preservation of antigens or other features of the cells is desired.

Product Specific Literature References

Detection of 5-bromo-2-deoxyuridine incorporated into DNA by labeling strand breaks induced by photolysis (SBIP): X. Li, et al.; Int. J. Oncol. 4, 1157 (1994)
Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color: X. Li, et al.; Exp. Cell. Res. 222, 28 (1996) Abstract

General Information

MANUFACTURER

Manufactured by Phoenix Flow Systems.

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Detection Kits

ALX-850-041

Revised 29-Jan-05

APO-DIRECT™ Kit
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	DNA-based Apoptosis Detection

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-041-KI01		1 Kit	540.00 USD

Product Specification

QUANTITY:	50 assays.
KIT/SET CONTAINS:	Contains all the reagents necessary, including positive and negative control cells.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	+4°C
POSITIVE CONTROL INCLUDED:	yes

Product Description

One-step flow cytometry kit for the qualitative and quantitative measurement of apoptosis in mammalian cells. One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. The large number of DNA fragments appearing in apoptotic cells results in a multitude of 3'-hydroxyl termini of DNA ends which can be identified with fluorescent tagged deoxyuridine triphosphate nucleotides (F-dUTP). The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes a template independent addition of deoxyribonucleoside triphosphates (e.g. F-dUTP) to the 3'h-hydroxyl ends of double- or single-stranded DNA. A substantial number of these sites are available in apoptotic cells providing the basis for the single step fluorescent labelling and flow cytometric method utilized in the Apo-DIRECT™ Kit.

Product Specific Literature References

Features of apoptotic cells measured by flow cytometry: Z. Darzynkiewicz, et al.; Cytometry 13, 795 (1992) Abstract
Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis): Z. Darzynkiewicz, et al.; Cytometry 27, 1 (1997), (Review) Abstract
Single-step procedure for labeling DNA strand breaks with fluorescein- or BODIPY-conjugated deoxynucleotides: detection of apoptosis and bromodeoxyuridine incorporation: X. Li, et al.; Cytometry 20, 172 (1995) Abstract

General Information

MANUFACTURER

Manufactured by Phoenix Flow Systems.

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Detection Kits

ALX-804-192

Revised 30-Jul-07

Monoclonal Antibody to ssDNA (F7-26)
SYNONYMS	anti-Single-stranded DNA MAb (F7-26)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	DNA Fragmentation & Chromatin Condensation

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-192-R200		200 µl	190.00 USD
ALX-804-192-L001		1 ml	430.00 USD

Product Specification

CLONE:	F7-26
ISOTYPE:	Mouse IgM
QUANTITY:	200µl/20 tests; 1ml/100 tests
CONCENTRATION:	0.1mg/ml
FORMULATION:	Liquid. In PBS containing 5% fetal bovine serum (FBS).
IMMUNOGEN:	Calf thymus ssDNA.
SPECIFICITY:	Recognizes deoxycytidine of ssDNA of at least 25-30 bases in length in the absence of any reactivity to double-stranded DNA; not species-specific. Specifically detects apoptotic cells; necrotic cells are not recognized.
APPLICATION:	Flow Cytometry Immunocytochemistry Immunohistochemistry (frozen sections, paraffin sections - important: applicable for cold fixation at +4°C - for reference see Manual).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C

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Product Description

This reagent (MAb F7-26) allows the important discrimination between apoptosis and necrosis [1-3,6,7]. In this respect it can be regarded as a unique research tool superior to TUNEL staining [4,5]. The specificity of the assay is based upon the high sensitivity of DNA to thermal denaturation in condensed chromatin of apoptotic cells.

Although there is no change of the original product there have been important improvements in the staining protocol involving a formamide-based DNA-denaturation [8]. This is now more convenient to use, yielding a sharper contrast between apoptotic cells and controls. The increased sensitivity of MAb F7-26 allows omission of a previously recommended Biotin/Streptavidin amplification step, a source of background problems. The APOSTAIN assay is now applicable for cold (4°C) formalin fixed tissue. For recent applications of MAb F7-26 see [9-15].

Product Specific Literature References

[1] Detection of DNA damage in individual cells by flow cytometric analysis using anti-DNA monoclonal antibody: O.S. Frankfurt; Exp. Cell. Res. 170, 369 (1987) Abstract
[2] Detection of apoptosis in leukemic and breast cancer cells with monoclonal antibody to single-stranded DNA: O.S. Frankfurt; Anticancer Res. 14, 1861 (1994) Abstract
[3] Decreased stability of DNA in cells treated with alkylating agents: O.S. Frankfurt; Exp. Cell. Res. 191, 181 (1990) Abstract
[4] A cautionary note on the use of the TUNEL stain to determine apoptosis: C. Charriaut-Marlangue & J. Ben-Ari; Neuroreport 7, 61 (1995) Abstract
[5] In Situ detection of fragmented DNA (TUNEL assay) fails to discriminate among apoptosis, necrosis and autolytic cell death: A cautionary note: B. Grasl-Kraupp, et al.; Hepatology 21, 1465 (1995) Abstract
[6] Monoclonal antibody to single-stranded DNA is a specific and sensitive cellular marker of apoptosis: O.S. Frankfurt, et al.; Exp. Cell. Res. 226, 387 (1996) Abstract
[7] Detection of apoptotic cells with monoclonal antibodies to single-stranded DNA: O.S. Frankfurt; Apoptosis Detection and Assay Methods (Eds L. Zhu & J. Chun) 47 (1998)
[8] Identification of apoptotic cells by formamide-induced dna denaturation in condensed chromatin: O.S. Frankfurt & A. Krishan; J. Histochem. Cytochem. 49, 369 (2001) Abstract
[9] Apoptosis: one of the mechanisms that maintains unresponsiveness of the intestinal mucosal immune system: P. Bu, et al.; J. Immunol. 166, 6399 (2001) Abstract
[10] Effects of dexamethasone on apoptosis-related cell death after spinal cord injury: M. Zurita, et al.; J. Neurosurg. 96, 83 (2002) Abstract
[11] Imbalance between apostain expression and proliferative index can predict survival in primary glioblastoma: J. Vaquero, et al.; Acta Neurochir. (Wien) 144, 151 (2002) Abstract
[12] Early T-cell apoptosis and Fas expression during antiretroviral therapy in individuals infected with human immunodeficiency virus-1: S.P. Aries, et al.; Scand. J. Immunol. 48, 86 (1998) Abstract
[13] DNA strand breaks in Alzheimer's disease: E. Adamec, et al.; Brain Res. 849, 67 (1999) Abstract
[14] In vivo amifostine (WR-2721) prevents chemotherapy-induced apoptosis of peripheral blood lymphocytes from cancer patients: M. Provinciali, et al.; Life Sci. 64, 1525 (1999) Abstract
[15] Prediction of imminent complications in HIV-1-infected patients by markers of lymphocyte apoptosis: J.C. Wasmuth, et al.; J. Acquir. Immune Defic. Syndr. 23, 44 (2000) Abstract
[16] Caspase-independent phosphatidylserine exposure during apoptosis of primary T lymphocytes: C. Ferraro-Peyret, et al.; J. Immunol. 169, 4805 (2002) Abstract; Full Text
[17] Cathepsin-B-dependent apoptosis triggered by antithymocyte globulins: a novel mechanism of T-cell depletion: M. C. Michallet, et al.; Blood 102, 3719 (2003) Abstract

General Information

MANUFACTURER

Manufactured by Apostain, Inc.

Further Categories Containing This Product:

DNA-based Apoptosis Detection • DNA Repair Other Products • Monoclonal Antibodies

ALX-210-390

Revised 27-Sep-05

Polyclonal Antibody to Histone γ-H2AX (human) (phosphorylated) (pSer139)
SYNONYMS	anti-Histone H2AFX (human) (phosphorylated) (pSer¹³⁹) PAb anti-Histone H2A.X (human) (phosphorylated) (pSer¹³⁹) PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Histones / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-390-R050		50 µl	150.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	From rabbit.
CONCENTRATION:	0.67mg/ml.
FORMULATION:	Liquid. Affinity purified antibody in PBS containing 0.01% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to human histone γ-H2AX (K¹³⁴ATQApSQEY¹⁴²) phosphorylated at Ser¹³⁹.
SPECIFICITY:	Recognizes Ser¹³⁹-phosphorylated human histone γ-H2AX. Does not cross-react with non-phosphorylated human histone γ-H2AX.
APPLICATION:	Immunocytochemistry Western Blot
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C

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Product Specific Literature References

DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139: E.P. Rogakou, et al.; J. Biol. Chem. 273, 5858 (1998) Abstract; Full Text
Hyperphosphorylation of histone H2A.X and dephosphorylation of histone H1 subtypes in the course of apoptosis: H. Talasz, et al.; Cell Death Differ. 9, 27 (2002) Abstract
Local DNA damage by proton microbeam irradiation induces poly(ADP-ribose) synthesis in mammalian cells: L. Tartier, et al.; Mutagenesis 18, 411 (2003) Abstract
Conserved histone variant H2A.Z protects euchromatin from the ectopic spread of silent heterochromatin: M.D. Meneghini, et al.; Cell 112, 725 (2003) Abstract

General Literature References

SET-ting the stage for life and death: D. Chakravarti and R. Hong; Cell 112, 589 (2003) Abstract
Cancer: protective packaging for DNA: J.A. Downs and S.P. Jackson; Nature 424, 732 (2003) Abstract

Further Categories Containing This Product:

Phospho-specific Antibodies • DNA-based Apoptosis Detection • Polyclonal Antibodies

ALX-850-242

Revised 28-Apr-05

Quick Apoptotic DNA Ladder Detection Kit
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	DNA-based Apoptosis Detection

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-242-KI01		1 Kit	315.00 USD

Product Specification

QUANTITY:	50 tests.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Images

Please click on thumbnails to enlarge.

Product Description

The Quick Apoptotic DNA Ladder Detection Kit offers fast and sensitive detection of DNA fragmentation in apoptotic cells. Unlike other similar kits on the market which require 1-2 days to prepare nucleic acids, the new procedure requires less than 90 minutes to prepare chromosomal DNA in a single tube, with no need for extraction or a column. DNA ladders can easily be visualized by agarose gel electrophoresis. The new procedure increases recovery of small fragmented DNA, and therefore improves detection sensitivity.

General Information

MANUFACTURER

Manufactured by BioVision.

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