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Nitric Oxide & NOS Detection / Related Products
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ALX-400-004 Revised 29-Aug-08
Griess Reagent
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-400-004-L050   50 ml 70.00 USD Add To Cart
Product Specification
APPEARANCE: Colorless to slightly pink liquid.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HAZARD: CORROSIVE.
Product Description
Reagent for the determination of nitrite (NO2-) in biological media. Consists of 1 part 0.1% naphthylethylenediamine dihydrochloride in distilled water plus 1 part 1% sulfanilamide (or sulfanilic acid) in 5% concentrated H3PO4.
Product Specific Literature References
Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids: L.C. Green, et al.; Anal. Biochem. 126, 131 (1982) Abstract
Measurement of nitrate and nitrite in biological samples using nitrate reductase and Griess reaction: D.L. Granger, et al.; Methods Enzymol. 268, 142 (1996) Abstract
Anticoagulants and other preanalytical factors interfere in plasma nitrate/nitrite quantification by the Griess method: D. Ricart-Jane, et al.; Nitric Oxide 6, 178 (2002) Abstract
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Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-610-001 Revised 23-Feb-07
2,3-Diaminonaphthalene
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SYNONYMS DAN
2,3-Naphthalenediamine
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-610-001-M100   100 mg 40.00 USD Add To Cart
Product Specification
FORMULA: C10H10N2
MW: 158.2
CAS NUMBER: 771-97-1
PURITY: ≥95% (GC)
APPEARANCE: Off-white to light brown solid.
SOLUBILITY: Soluble in pyridine, DMSO or dimethylformamide.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HAZARD: MAY BE CARCINOGENIC. TOXIC.

Product Description
Inhibitor of nitric oxide (NO) formation. The relatively nonfluorescent DAN reacts rapidly with the NO-derived N-nitrosating agent(s) to yield the highly fluorescent product 2,3-naphthotriazole (NAT).
Product Specific Literature References
A fluorometric assay for the measurement of nitrite in biological samples: T.P. Misko, et al.; Anal. Biochem. 214, 11 (1993) Abstract
Determination of nitric oxide using fluorescence spectroscopy: A.M. Miles, et al.; Meth. Enzymol. 268, 105 (1996) Abstract
Determination of selectivity of reactive nitrogen oxide species for various substrates: D.A. Wink, et al.; Meth. Enzymol. 268, 120 (1996) Abstract
Intensity-independent fluorometric detection of cellular nitric oxide release: P.J. Andrew, et al.; FEBS Lett. 408, 319 (1997) Abstract
Improved methods to measure end products of nitric oxide in biological fluids: nitrite, nitrate, and S-nitrosothiols: M. Marzinzig, et al.; Nitric Oxide 1, 177 (1997) Abstract
Improved nitric oxide detection using 2,3-diaminonaphthalene and its application to the evaluation of novel nitric oxide synthase inhibitors: N. Nakatsubo, et al.; Biol. Pharm. Bull. 21, 1247 (1998) Abstract
The oxidative and nitrosative chemistry of the nitric oxide/superoxide reaction in the presence of bicarbonate: D. Jourd'heuil, et al.; Arch. Biochem. Biophys. 365, 92 (1999) Abstract
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Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-610-002 Revised 05-Dec-06
Luminol
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SYNONYMS 5-Amino-2,3-dihydro-1,4-phthalazinedione
3-Aminophthalhydrazide
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-610-002-G001   1 g 25.00 USD Add To Cart
ALX-610-002-G005   5 g 65.00 USD Add To Cart
Product Specification
FORMULA: C8H7N3O2
MW: 177.2
CAS NUMBER: 521-31-3
MERCK INDEX: 14: 5600
RTECS: TH8890060
PURITY: ≥98%
APPEARANCE: Off-white to brown solid.
SOLUBILITY: Soluble in DMSO or in 10% ammonia (hot).
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HANDLING: Protect from light.
HAZARD: IRRITANT.

Product Description
Key reagent for generating luminescence [1]. A reagent for the highly sensitive and selective detection of nitric oxide (NO) in biological samples by a luminol-H2O2 chemiluminescence method [2-3].
Product Specific Literature References
[1] D.F. Roswell & E.H. Shite; Meth. Enzymol. 57, 409 (1978), (Review)
[2] New method of detecting nitric oxide production: K. Kikuchi, et al.; Chem. Pharm. Bull. 40, 2233 (1992) Abstract
[3] Detection of nitric oxide production from a perfused organ by a luminol- H2O2 system: K. Kikuchi, et al.; Anal. Chem. 65, 1794 (1993) Abstract
[4] Real time measurement of nitric oxide produced ex vivo by luminol-H2O2 chemiluminescence method: K. Kikuchi, et al.; J. Biol. Chem. 268, 23106 (1993) Abstract; Full Text
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Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-610-003 Revised 20-Jun-08
Methylene blue . trihydrate
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SYNONYMS 3,7-bis(Dimethylamino)phenothiazin-5-ium chloride . 3H2O
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-610-003-G005   5 g 20.00 USD Add To Cart
Product Specification
FORMULA: C16H18ClN3S . 3H2O
MW: 319.9 . 54.1
CAS NUMBER: 7220-79-3
MERCK INDEX: 14: 6060
RTECS: SP5740000
APPEARANCE: Green crystalline powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HAZARD: HARMFUL. POTENT NEUROTOXIN.

Product Description
Inhibitor of soluble guanylyl cyclase (sGC). Biological staining agent. Spin-trap which forms stable adducts with oxygen free radicals in solution.
Product Specific Literature References
Novel actions of methylene blue: B. Mayer, et al.; Eur. Heart J 14 Suppl I, 22 (1993) Abstract
Inhibition of nitric oxide synthesis by methylene blue: B. Mayer, et al.; Biochem. Pharmacol. 45, 367 (1993) Abstract
Modulation of nitric oxide-dependent relaxation of pig tracheal smooth muscle by inhibitors of guanylyl cyclase and calcium activated potassium channels: M.S. Kannan & D.E. Johnson; Life Sci. 56, 2229 (1995) Abstract
Methylene blue induces cytotoxicity in human brain tumor cells: Y.S. Lee & R.D. Wurster; Cancer Lett. 88, 141 (1995) Abstract
Methylene blue is a muscarinic antagonist in cardiac myocytes: N. Abi-Gerges, et al.; Mol. Pharmacol. 52, 482 (1997) Abstract
A comparative study of the effects of three guanylyl cyclase inhibitors on the L-type Ca2+ and muscarinic K+ currents in frog cardiac myocytes: N. Abi-Gerges, et al.; Br. J. Pharmacol 121, 1369 (1997) Abstract
Methylene blue inhibits stimulatory effect of sodium nitroprusside but not of 3-morpholino sydnonimine on prolactin secretion in freely moving male rats: M.C. González & E. Llorente; Brain Res. Bull. 46, 229 (1998) Abstract
Comparison between endothelial and neuronal nitric oxide pathways in rat aorta and gastric fundus: C. Guilmard, et al.; Nitric Oxide 2, 147 (1998) Abstract
Comparison of two soluble guanylyl cyclase inhibitors, methylene blue and ODQ, on sodium nitroprusside-induced relaxation in guinea-pig trachea: T.L. Hwang, et al.; Br. J. Pharmacol 125, 1158 (1998) Abstract
Methylene Blue--a therapeutic dye for all seasons?: M. Wainwright & K.B. Crossley; J. Chemother. 14, 431 (2002), (Review) Abstract
Methylene blue photoinactivation of RNA viruses: R.A. Floyd, et al.; Antiviral Res. 61, 141 (2004), (Review) Abstract
Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways: H. Atamna, et al.; FASEB J. 22, 703 (2008) Abstract
Further Categories Containing This Product:
Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products • Neurotoxins • Spin Traps, Spin Probes & Spin Labels • Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-610-023 Revised 10-Jul-08
DAN-1 EE . hydrochloride
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SYNONYMS 4-((3-Amino-2-naphthalenyl)aminomethyl)benzoic acid ethyl ester . HCl
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-610-023-M005   5 mg 162.00 USD Add To Cart
Product Specification
FORMULA: C20H20N2O2 . HCl
MW: 320.4 . 36.5
PURITY: ≥95%
FORMULATION: Liquid. Solution in ethanol.
SOLUBILITY: 30mg/ml soluble in DMSO or dimethyl formamide; slightly soluble in PBS, pH 7.2 (0.5mg/ml).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C. We do not recommend storing aqueous solutions for more than one day.

Product Description
Fluorescent indicator for the bioimaging of nitric oxide (NO). Upon entry into the cell, DAN-1 EE is transformed by cytosolic esterases into the less cell permeable DAN-1. Intracellular formation of NO can be monitored (Ex(max): 360-380nm; Em(max): 420-450nm).
Product Specific Literature References
Development of a fluorescent indicator for the bioimaging of nitric oxide: H. Kojima, et al.; Biol. Pharm. Bull. 20, 1229 (1997) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION To change the solvent, evaporate the ethanol under a gentle stream of nitrogen and immediately add the solvent of choice.
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Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-620-052 Revised 25-Sep-08
DAF-2
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SYNONYMS 4,5-Diaminofluorescein
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-620-052-M001   1 mg 360.00 USD Add To Cart
Product Specification
FORMULA: C20H16N2O5
MW: 364.3
CONCENTRATION: ~5mM
PURITY: ≥98%
FORMULATION: Pale yellow liquid. In 0.55ml DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING: Keep under inert gas. After opening, prepare aliquots and store at -20°C. Protect from light.
HAZARD: IRRITANT.

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Product Description
Highly sensitive fluorescent probe for the real-time detection of nitric oxide (NO) in vivo. In the cell, the product reacts rapidly with NO in the presence of O2 to form the highly fluorescent compound triazolofluorescein (DAF-2T (Prod. No. ALX-620-060), Ex 495nm; Em 515nm). Not for sale in Japan.
Product Specific Literature References
Development of a fluorescent indicator for nitric oxide based on the fluoroscein chromophore: H. Kojima, et al.; Chem. Pharm. Bull. 46, 373 (1998) Abstract
Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport 9, 3345 (1998) Abstract
Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins: H. Kojima, et al.; Anal. Chem. 70, 2446 (1998) Abstract
Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins: N. Nakatsubo, et al.; FEBS Lett. 427, 263 (1998) Abstract
Hypotonic stress-induced NO production in endothelium depends on endogenous ATP: C. Kimura, et al.; BBRC 274, 736 (2000) Abstract
Anti-NO action of carvedilol in cell-free system and in vascular endothelial cells: T. Yoshioka, et al.; Br. J. Pharmacol. 129, 1530 (2000) Abstract
Fluorescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000)
Formation of peroxynitrite in vascular endothelial cells exposed to cyclosporine A: J. Navarro-Antolin, et al.; FASEB J. 15, 1291 (2001) Abstract
Impairment of endothelial nitric oxide production by acute glucose overload: C. Kimura, et al.; Am. J. Physiol. Endocrinol. Metab. 280, E171 (2001) Abstract
Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging: M. Broillet, et al.; FEBS Lett. 491, 227 (2001) Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-620-054 Revised 11-Oct-06
4AF DA
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SYNONYMS 4-Aminofluorescein diacetate
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-620-054-M001   1 mg 136.00 USD Add To Cart
Product Specification
FORMULA: C24H17NO7
MW: 431.4
PURITY: ≥99% (HPLC)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Protect from light. Keep under inert gas.

Product Description
Negative control compound for the fluorescent probes DAF-2 (Prod. No. ALX-620-052) and DAF-2 DA (Prod. No. ALX-620-056).
Product Specific Literature References
Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport 9, 3345 (1998) Abstract
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Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-620-056 Revised 25-Sep-08
DAF-2 DA (cell permeable)
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SYNONYMS 4,5-Diaminofluorescein . diacetate
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-620-056-M001   1 mg 360.00 USD Add To Cart
Product Specification
FORMULA: C24H18N2O7
MW: 446.4
CONCENTRATION: ~5mM
PURITY: ≥99%
FORMULATION: Liquid. In 0.45ml DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING: Keep under inert gas. Protect from light. After opening, prepare aliquots and store at -20°C.

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Product Description
Cell permeable analog of DAF-2 (Prod. No. ALX-620-052) that is hydrolyzed to DAF-2 by intracellular esterases. Can be used in fluorescence microscopy to measure real-time changes in nitric oxide (NO) levels in vivo (Ex(max): 495nm; Em(max): 515nm). Not for sale in Japan.
Product Specific Literature References
Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport 9, 3345 (1998) Abstract
Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins: H. Kojima, et al.; Anal. Chem. 70, 2446 (1998) Abstract
Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins: N. Nakatsubo, et al.; FEBS Lett. 427, 263 (1998) Abstract
Development of a fluorescent indicator for nitric oxide based on the fluoroscein chromophore: H. Kojima, et al.; Chem. Pharm. Bull. 46, 373 (1998) Abstract
Hypotonic stress-induced NO production in endothelium depends on endogenous ATP: C. Kimura, et al.; BBRC 274, 736 (2000) Abstract
Anti-NO action of carvedilol in cell-free system and in vascular endothelial cells: T. Yoshioka, et al.; Br. J. Pharmacol. 129, 1530 (2000) Abstract
Fluroescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000)
Formation of peroxynitrite in vascular endothelial cells exposed to cyclosporine A: J. Navarro-Antolin, et al.; FASEB J. 15, 1291 (2001) Abstract
Impairment of endothelial nitric oxide production by acute glucose overload: C. Kimura, et al.; Am. J. Physiol. Endocrinol. Metab. 280, E171 (2001) Abstract
Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging: M. Broillet, et al.; FEBS Lett. 491, 227 (2001) Abstract
Nitric Oxide Ameliorates Hydrophobic Bile Acid-induced Apoptosis in Isolated Rat Hepatocytes by Non-mitochondrial Pathways: E. Gumpricht, et al.; J. Biol. Chem. 277, 25823 (2002) Abstract; Full Text
BMP controls nitric oxide-mediated regulation of cell numbers in the developing neural tube: A. Traister, et al.; Cell Death Differ. (2004) Abstract
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Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-620-060 Revised 25-Sep-08
DAF-2T
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SYNONYMS Diaminofluorescein-2T
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-620-060-C100   100 µg 160.00 USD Add To Cart
Product Specification
FORMULA: C20H11N3O5
MW: 373.3
CONCENTRATION: ~0.5mM
PURITY: ≥98% (HPLC)
FORMULATION: Pale yellow liquid. In 0.54ml DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING: After opening, prepare aliquots and store at -20°C. Protect from light. Keep under inert gas.

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Product Description
Fluorescent compound (Ex(max): 495nm; Em(max): 515nm) produced by the reaction between DAF-2 (Prod. No. ALX-620-052) and nitric oxide (NO). Control compound for NO detection using DAF-2 (Prod. No. ALX-620-052) or DAF-2 DA (cell permeable) (Prod. No. ALX-620-056). Not for sale in Japan.
Product Specific Literature References
Fluorescent indicators for imaging nitric oxide production: H. Kojima, et al.; Angew. Chem. Int. Ed. Engl. 38, 3209 (1999) Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-620-067 Revised 25-Sep-08
DAR-4M
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SYNONYMS Diaminorhodamine-4M
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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