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ALX-201-065
Revised 18-Sep-08
MGMT (human) (recombinant)
SYNONYMS
O
6
-Methylguanine-DNA Methyltransferase (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Repair Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-065-R050
50 µl
320.00 USD
Product Specification
EC:
2.1.1.63
SOURCE/HOST:
Produced in
E. coli
. Full length human MGMT is fused at the N-terminus to a GST-tag.
PURITY:
Purified with Glutathiose-Sepharose 4B and thrombin cleavage
FORMULATION:
Liquid. 5'000U in 50µl buffer containing 50% glycerol, 100µg/ml BSA, 20mM TRIS-HCl, pH 7.8, 5mM dithiothreitol, 60mM KCl and 1mM EDTA.
SPECIFIC ACTIVITY:
100U/µl. One unit is defined as the amount of enzyme that removes 100fmol O
6
-methylguanine from DNA.
QUALITY CONTROL:
The biological activity has been checked in a radioactive standard MGMT assay.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Do not store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P16455:
MGMT (human)
Further Categories Containing This Product:
DNA & RNA Methyl & Alkyl Transferases / Related Products
•
Recombinant Proteins / Fusion Proteins
ALX-201-178
Revised 17-May-06
SET7/9 (human) (recombinant)
SYNONYMS
Histone H3-K4 Methyltransferase (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA & RNA Methyl & Alkyl Transferases / Related Products
Ordering Information
Product Numbers:
Format:
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Unit Price:
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ALX-201-178-C100
100 µg
125.00 USD
Product Specification
MW:
~40.7kDa.
EC:
2.1.1.43
SOURCE/HOST:
Produced in
E. coli.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 0.2M sodium chloride, 5mM DTT and 20% glycerol.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet:
T. Kwon, et al.; EMBO J.
22
, 292 (2003)
Abstract
General Information
Histone methyltransferase that transfers methyl groups to Lys
4
of histone H3, in complex with
S
-adenosyl-L-methionine.
General Literature References
Structure and catalytic mechanism of the human histone methyltransferase SET7/9
:
B. Xiao, et al.; Nature
421
, 652 (2003)
Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-210-228
Revised 05-Jun-07
Polyclonal Antibody to p300 (human)
SYNONYMS
anti-E1A Binding Protein p300 (human) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
p300 / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-228-C100
100 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 139-151 (G
139
TSGPNQGPTQST
151
C) in the nuclear factor binding domain of human p300 (E1A binding protein p300).
SPECIFICITY:
Recognizes human p300. Detects a band of ~265kDa by Western blot.
APPLICATION:
Western Blot (1-2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-165-038
.
General Information
p300 is a transcriptional coactivator closely related to CREB binding protein (CBP), interacting with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), cFos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB, and MyoD. p300 displays histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.
Related Products
ALX-165-038
Blocking Peptide for PAb to p300 (human) (Prod. No.
ALX-210-228
)
Further Categories Containing This Product:
Transcriptional Coactivators / Related Products
•
Polyclonal Antibodies
ALX-210-243
Revised 20-Oct-04
Polyclonal Antibody to CLOCK (NT)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Circadian Transcriptional Regulators / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
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ALX-210-243-R200
200 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Others
SOURCE/HOST:
From rabbit.
FORMULATION:
100µg of epitope affinity purified rabbit IgG (1.0mg/ml) with 1.0mg/ml BSA and 0.05% sodium azide in PBS.
IMMUNOGEN:
Synthetic peptide corresponding to aa 11-30 of mouse CLOCK.
SPECIFICITY:
Recognizes mouse, rat and hamster CLOCK. Detects a band of ~100kDa by Western blot.
APPLICATION:
Immunohistochemistry
Western Blot (1:200)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-153-031
.
Product Description
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein mediated autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-BMAL1 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of
Drosophila per
. PER, in concert with the product of the mammalian
timeless
gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-244
Revised 20-Oct-04
Polyclonal Antibody to CLOCK (mouse) (CT)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Circadian Transcriptional Regulators / Related Products
Ordering Information
Product Numbers:
Format:
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Unit Price:
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ALX-210-244-R200
200 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST:
From rabbit.
FORMULATION:
100µg of epitope affinity purified rabbit IgG (1.9mg/ml) with 1.0mg/ml BSA and 0.05% sodium azide in PBS.
IMMUNOGEN:
Synthetic peptide corresponding to aa 394-411 of mouse CLOCK.
SPECIFICITY:
Recognizes mouse CLOCK. Detects a band of ~100kDa by Western blot.
APPLICATION:
Western Blot (1:200)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-153-032
.
Product Description
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein mediated autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-BMAL1 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of
Drosophila per
. PER, in concert with the product of the mammalian
timeless
gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-245
Revised 21-Nov-06
Polyclonal Antibody to Ah Receptor Nuclear Translocator 3
SYNONYMS
anti-ARNT3 PAb
anti-BMAL1 PAb
anti-Brain & Muscle ARNT-like 1 PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Aryl Hydrocarbon Receptor / Related Products
Ordering Information
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Format:
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ALX-210-245-R200
200 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 582-594 (CD
582
MIDNDQG
SSSPS
594
) of mouse ARNT3 (Ah receptor nuclear translocator 3).
SPECIFICITY:
Recognizes human and hamster ARNT3.
APPLICATION:
Western Blot (1:200)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-152-023
.
General Information
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein mediated autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-BMAL1 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK-BMAL1 has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian the timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
Further Categories Containing This Product:
Circadian Transcriptional Regulators / Related Products
•
Polyclonal Antibodies
ALX-210-246
Revised 14-Sep-06
Polyclonal Antibody to PER1 (mouse)
SYNONYMS
anti-Period Circadian Protein 1 (mouse) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcriptional Repressors & Corepressors / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-246-R200
200 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 39-51 (P
39
SLADDTDANSNG
51
) of mouse PER1 (period circadian protein 1).
SPECIFICITY:
Recognizes mouse PER1.
APPLICATION:
Immunohistochemistry (frozen and paraffin sections (1:500))
Western Blot (1:200)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-165-043
.
Product Description
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein mediated autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Tranasactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-BMAL1 heterodimers bind to E box regulatory elements and stimulate gene transcription. PER, when complexed with the product of the mammalian
timeless
gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
Further Categories Containing This Product:
Circadian Transcriptional Regulators / Related Products
•
Polyclonal Antibodies
ALX-210-253
Revised 12-Jul-07
Polyclonal Antibody to SMRT (human)
SYNONYMS
anti-Silencing Mediator of Retinoic Acid and Thyroid Hormone Receptor (human) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
SMRT & N-CoR / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-253-C100
100 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 1302-1495 of human SMRT (silencing mediator of retinoic acid and thyroid hormone receptor).
SPECIFICITY:
Recognizes human SMRT. Detects a band of ~250kDa by Western blot.
APPLICATION:
Immunoprecipitation, Chromatin Immunoprecipitation
Western Blot (2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
General Information
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. SMRT (silencing mediator of retinoic acid & thyroid hormone receptor) and N-CoR (nuclear receptor corepressor) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-254
Revised 01-Feb-07
Polyclonal Antibody to N-CoR
SYNONYMS
anti-Nuclear Receptor Co-repressor PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
SMRT & N-CoR / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-254-C050
50 µg
524.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST:
From rabbit.
CONCENTRATION:
0.7mg/ml
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 2437-2453 (CP
2437
APLLSAQYETLSDSDD
2453
) of mouse N-CoR (nuclear receptor co-repressor).
SPECIFICITY:
Recognizes human and mouse N-CoR.
APPLICATION:
Immunoprecipitation, Chromatin Immunoprecipitation
Western Blot (1µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-163-007
.
General Information
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. SMRT (silencing mediator of retinoic acid & thyroid hormone receptor) and N-CoR (nuclear receptor corepressor) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-284
Revised 13-Jan-05
Polyclonal Antibody to RIP140 (human)
SYNONYMS
anti-Receptor-interacting Protein 140 (human) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Regulators Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-284-C100
100 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 718-735 (G
718
NPTKGRVKKK
EKTPLRD
735
) of human RIP140 (receptor-interacting protein 140).
SPECIFICITY:
Recognizes human RIP140. Detects a band of ~140kDa by Western blot.
APPLICATION:
Immunoprecipitation
Western Blot (2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-166-005
.
Product Description
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Receptor-Interacting Protein 140, also known as RIP140, has been shown to enhance estrogen receptor transcriptional activity through the AF2-AD domain in an agonist dependent manner and has also been implicated to be involved in other steroid receptor pathways, including the retinoid pathway. RIP140 has recently been shown to interact with peroxisome proliferator-activated receptor-α (PPARα) and has been proposed to competitively inhibit PPARα interaction with another known nuclear receptor coactivator, SRC-1. However, the specific roles for coactivation and corepression by RIP140 in steroid receptor pathways is currently not well understood.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-285
Revised 30-Oct-06
Polyclonal Antibody to SMRT (human)
SYNONYMS
anti-Silencing Mediator of Retinoic Acid and Thyroid Hormone Receptor (human) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
SMRT & N-CoR / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-285-C100
100 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Recombinant human SMRT (silencing mediator of retinoic acitd and thyroid hormone receptor) (aa 95-565).
SPECIFICITY:
Recognizes human SMRT. Detects a band of ~250kDa by Western blot.
APPLICATION:
Western Blot (4µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Polyclonal Antibodies
