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Viral Signalling
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ALX-803-060 Revised 27-Apr-06
Monoclonal Antibody to NS5B (HCV) (5B-3B1)
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SYNONYMS anti-Nonstructural Protein 5B (HCV) MAb (5B-3B1)
PRODUCT LINE Viral Signalling
PRODUCT CATEGORY Hepatitis Viruses / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-803-060-R100   100 µl 360.00 USD Add To Cart
Product Specification
CLONE: 5B-3B1
ISOTYPE: Mouse IgG2b
FORMULATION: Liquid. Ascites fluid diluted in PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase.
SPECIFICITY: Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382).
APPLICATION: Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase: D. Moradpour, et al.; J. Biol. Chem. 277, 593 (2002) Abstract; Full Text
Hepatitis C virus RNA synthesis in a cell-free system isolated from replicon-containing hepatoma cells: R.W. Hardy, et al.; J. Virol. 77, 2029 (2003) Abstract; Full Text
Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs: G. Randall, et al.; PNAS 100, 235 (2003) Abstract; Full Text
Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids: S.B. Kapadia and F.V. Chisari; PNAS 102, 2561 (2005) Abstract; Full Text
Quantitative analysis of the hepatitis C virus replication complex: D. Quinkert, et al.; J. Virol. 79, 13594 (2005) Abstract
Complete translation of the hepatitis C virus genome in vitro: membranes play a critical role in the maturation of all virus proteins except for NS3: Y.V. Svitkin, et al.; J. Virol. 79, 6868 (2005) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation
General Literature References
Unravelling hepatitis C virus replication from genome to function: B.D. Lindenbach and C.M. Rice; Nature 436, 933 (2005) Abstract
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Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-495 Revised 22-Dec-06
Monoclonal Antibody to Pin1 (8C10)
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SYNONYMS anti-Peptidyl-prolyl cis-trans Isomerase Pin1 MAb (8C10)
anti-PPIase Pin1 (8C10) MAb
anti-Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 MAb (8C10)
anti-PPIase NIMA-interacting 1 (8C10) MAb
anti-Rotamase Pin1 MAb (8C10)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Alzheimer Research Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-495-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 8C10
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN: Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1.
SPECIFICITY: Recognizes human, mouse and dog Pin1. Detects a band of ~16 kDa by Western blot.
APPLICATION: Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL INCLUDED: Yes (Prod. No. ALX-840-030).
General Information
Pin1, a member of the parvulin family of peptidyl-prolyl-cis-trans-isomerases (PPIases), specifically isomerizes only the phosphorylated  Ser/Thr-Pro bonds in certain proteins like Bcl-2, Cdc25C, Cdc27, p53 and Tau. Binding of Pin1 to p53 stimulates the DNA binding activity and transactivation function of p53. Pin1 also regulates the expression of cyclin D1 and is strikingly overexpressed in a subset of human tumors like breast cancer and oral squamous cell carcinoma.
Further Categories Containing This Product:
Stress & Heat Shock Proteins Other Products • Antiviral Signalling / Related Products • Monoclonal Antibodies • p53 Other Products
 
 
ALX-804-849 Revised 16-Aug-07
Monoclonal Antibody to RIG-I (Alme-1)
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SYNONYMS anti-RIG-1 MAb (Alme-1)
anti-Retinoic Acid-inducible Gene 1 Protein MAb (Alme-1)
anti-DEAD-box Protein 58 MAb (Alme-1)
anti-Probable ATP-dependent RNA Helicase DDX58 MAb (Alme-1)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
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ALX-804-849-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Alme-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIG-I (retinoic acid-inducible gene 1 protein) (aa 201-713).
SPECIFICITY: Recognizes human and mouse RIG-I.
APPLICATION: Immunohistochemistry (paraffin sections)
Immunoprecipitation (1:100)
Western blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Virology. Sensing viral RNA amid your own: T. Fujita; Science 314, 935 (2006) Abstract
5’-Triphosphate RNA is the ligand for RIG-I: V. Hornung, et al.; Science 314, 994 (2006) Abstract
RIG-I-mediated antiviral responses to single-stranded RNA bearing 5’-phosphates: A. Pichlmair, et al.; Science 314, 997 (2006) Abstract
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Further Categories Containing This Product:
Antiviral Signalling / Related Products • NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • Polyclonal Antibodies
 
 
ALX-804-372 Revised 22-May-08
Monoclonal Antibody to TANK-binding Kinase 1 (108A429)
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SYNONYMS anti-TBK1 MAb (108A429)
anti-NAK MAb (108A429)
anti-NF-κB-activating Kinase MAb (108A429)
anti-T2K MAb (108A429)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY IkB Kinases [IKKs] / Related Products
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ALX-804-372-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 108A429
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 563-577 (YYQFKKDKAERRLAYC) of human TBK1 (TANK-binding kinase 1).
SPECIFICITY: Recognizes human, mouse, rat, dog and bovine TBK1. Detects a band of ~80kDa by Western blot.
APPLICATION: Western Blot (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
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Product Description
TANK-binding Kinase 1 (TBK1) is a member of the IKK family. TANK (TRAF-associated NF-κB activator) interacts with TBK1 and activates NF-κB in a kinase-dependent manner [1].
Product Specific Literature References
[1] NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase: J.L. Pomerantz & D. Baltimore; EMBO J. 18, 6694 (1999) Abstract; Full Text
[2] IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway: K.A. Fitzgerald, et al.; Nat. Immunol. 4, 491 (2003) Abstract
[3] Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I: R. Sumpter, Jr., et al.; J. Virol. 79, 2689 (2005) Abstract; Full Text
[4] Gene expression and antiviral activity of alpha/beta interferons and interleukin-29 in virus-infected human myeloid dendritic cells: P. Osterlund, et al.; J. Virol. 79, 9608 (2005) Abstract
Further Categories Containing This Product:
Antiviral Signalling / Related Products • Monoclonal Antibodies
 
 
ALX-804-362 Revised 25-Sep-08
Monoclonal Antibody to Toll-like Receptor 3 (human) (40C1285.6)
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SYNONYMS anti-TLR3 MAb (40C1285.6)
anti-CD283 (human) MAb (40C1285.6)
PRODUCT LINE Inflammation
PRODUCT CATEGORY TLR Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-362-C100   100 µg 293.00 USD Add To Cart
ALX-804-362B-C100 Biotin 100 µg 319.00 USD Add To Cart
ALX-804-362F-C100 FITC 100 µg 358.00 USD Add To Cart
ALX-804-362R-C100 R-PE 100 µg 397.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 40C1285.6
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: 40C1285.6: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
40C1285.6-Biotin: Liquid. In PBS containing 0.02% sodium azide.
40C1285.6-FITC: Liquid. In 10mM TRIS containing 150mM sodium chloride and 0.02% sodium azide.
40C1285.6-R-PE: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptides corresponding to human TLR3 (Toll-like receptor 3) cytoplasmic portion.
SPECIFICITY: Recognizes human TLR3.
APPLICATION: Flow Cytometry (0.5-4µg/106 cells for intracellular staining, for cell surface see [7])
Immunocytochemistry (see [3],[8] and [9])
Immunohistochemistry (paraffin sections) (see [4] and [6])
Immunoprecipitation (2µg/106 transfected cells)
Western Blot (1-3µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep Biotin-, FITC- and R-PE-conjugated formats at +4°C. Do not freeze. Protect from light.
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Product Specific Literature References
[1] Subcellular localization of Toll-like receptor 3 in human dendritic cells: M. Matsumoto, et al.; J. Immunol. 171, 3154 (2003) Abstract
[2] The effect of innate immunity on autoimmune diabetes and the expression of Toll-like receptors on pancreatic islets: L. Wen, et al.; J. Immunol. 172, 3173 (2004) Abstract
[3] Regulation of the polymeric Ig receptor by signaling through TLRs 3 and 4: linking innate and adaptive immune responses: T.A. Schneeman, et al.; J. Immunol. 175, 376 (2005) Abstract
[4] Toll-like receptors-2, -3 and -4 expression patterns on human colon and their regulation by mucosal-associated bacteria: E. Furrie, et al.; Immunology 115, 565 (2005) Abstract
[5] Distinct poly(I-C) and virus-activated signaling pathways leading to interferon-beta production in hepatocytes: K. Li, et al.; J. Biol. Chem. 280, 16739 (2005 280, 16739 (2005) Abstract; Full Text
[6] Thyrocytes express a functional toll-like receptor 3: overexpression can be induced by viral infection and reversed by phenylmethimazole and is associated with Hashimoto’s autoimmune thyroiditis: N. Harii, et al.; Mol. Endocrinol. 19, 1231 (2005) Abstract
[7] Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils: C.K. Wong, et al.; Am. J. Respir. Cell Mol. Biol. 37, 85 (2007) Abstract
[8] Biochemical and functional analyses of the human Toll-like receptor 3 ectodomain: C.T. Ranjith-Kumar, et al.; J. Biol. Chem. 282, 7668 (2007) Abstract; Full Text
[9] Effects of single nucleotide polymorphisms on Toll-like receptor 3 activity and expression in cultured cells: C.T. Ranjith-Kumar, et al.; J. Biol. Chem. 282, 17696 (2007) Abstract; Full Text
Further Categories Containing This Product:
Antiviral Signalling / Related Products • Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-474 Revised 24-Mar-05
Monoclonal Antibody to Toll-like Receptor 3 (human) (TLR3.7)
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SYNONYMS anti-TLR3 (human) MAb (TLR3.7)
anti-CD283 (human) MAb (TLR3.7)
PRODUCT LINE Inflammation
PRODUCT CATEGORY TLR Antibodies
Ordering Information
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ALX-804-474-C050   50 µg 220.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: TLR3.7
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.2mg/ml.
FORMULATION: Liquid. Purified antibody in PBS containing 10% glycerol. Does not contain any preservatives.
IMMUNOGEN: Recombinant human TLR3 (Toll-like receptor 3).
SPECIFICITY: Recognizes human TLR3.
APPLICATION: Flow Cytometry
Immunocytochemistry
Immunoprecipitation
Functional Application (blocks double-stranded RNA-mediated signalling)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling: M. Matsumoto, et al.; BBRC 293, 1364 (2002) Abstract
Subcellular localization of Toll-like receptor 3 in human dendritic cells: M. Matsumoto, et al.; J. Immunol. 171, 3154 (2003) Abstract
Mechanism of up-regulation of human Toll-like receptor 3 secondary to infection of measles virus-attenuated strains: M. Tanabe, et al.; BBRC 311, 39 (2003) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Antiviral Signalling / Related Products • Monoclonal Antibodies • CD Antibodies
 
 
ALX-350-274 Revised 16-Jun-08
Myriocin
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SYNONYMS ISP-1
Thermozymocidin
2S,3R,4R,6E-2-Amino-3,4-dihydroxy-2-hydroxymethyl-14-oxo-6-eicosenoic acid
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Immunomodulators
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ALX-350-274-M005   5 mg 90.00 USD Add To Cart
Product Specification
FORMULA: C21H39NO6
MW: 401.5
CAS NUMBER: 35891-70-4
RTECS: JX3890000
PURITY: ≥98% (TLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO (25mg/ml; heat briefly in boiling water bath) or in dilute base (5mg/ml; 50mM NaOH).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HAZARD: HARMFUL.

Product Description
Potent immunosuppressant ~10 to 100-fold more potent than cyclosporin A (Prod. No. ALX-380-002). Blocks sphingolipid biosynthesis by inhibiting serine palmitoyltransferase (Ki=0.28nM). Induces apoptosis by depletion of cellular sphingolipids. Inhibits proliferation of an IL-2-dependent mouse cytotoxic cell line CTLL-1 (IC50=15nM).
Product Specific Literature References
Fungal metabolites. Part 11. A potent immunosuppressive activity found in Isaria sinclairii metabolite: T. Fujita, et al.; J. Antibiot. 47, 208 (1994) Abstract
Serine palmitoyltransferase is the primary target of a sphingosine-like immunosuppressant, ISP-1/myriocin: Y. Miyake, et al.; BBRC 211, 396 (1995) Abstract
Ceramide and its interconvertible metabolite sphingosine function as indispensable lipid factors involved in survival and dendritic differentiation of cerebellar Purkinje cells: S. Furuya, et al.; J. Neurochem. 71, 366 (1998) Abstract
Complete removal of sphingolipids from the plasma membrane disrupts cell to substratum adhesion of mouse melanoma cells: K. Hidari, et al.; J. Biol. Chem. 271, 14636 (1996) Abstract
Sli2 (Ypk1), a homologue of mammalian protein kinase SGK, is a downstream kinase in the sphingolipid-mediated signaling pathway of yeast: Y. Sun, et al.; Mol. Cell. Biol. 20, 4411 (2000) Abstract; Full Text
Effect of myriocin on plasma sphingolipid metabolism and atherosclerosis in apoE-deficient mice: M.R. Hojjati, et al.; J. Biol. Chem. 280, 10284 (2005) Abstract
Serine palmitoyltransferase inhibitor suppresses HCV replication in a mouse model: T. Umehara, et al.; BBRC 346, 67 (2006) Abstract
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Further Categories Containing This Product:
Natural Products - Apoptosis Inducers & Inhibitors • Sphingolipids & Glycosphingolipids Other Products • Natural Products - Antiviral / anti-HIV Agents
 
 
ALX-380-088 Revised 30-Jul-08
Netropsin . dihydrochloride
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SYNONYMS 4-([((Aminoiminomethyl)amino)acetyl)amino)-N-(5-(((3-amino-3-iminopropyl)amino)carbonyl]-1-methyl-1H-pyrrol-3-yl)-1-methyl-1H-pyrrole-3-carboxamide
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antitumor Antibiotics
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ALX-380-088-M005   5 mg 40.00 USD Add To Cart
Product Specification
FORMULA: C18H26N10O3 . 2HCl
MW: 430.5 . 73.0
CAS NUMBER: 63770-20-7 (hydrochloride)
RTECS: DW2973000
SOURCE/HOST: Isolated from Streptomyces netropsis.
PURITY: ≥93% (HPLC)
APPEARANCE: Faint yellow powder.
SOLUBILITY: Soluble in alcohols or water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
HAZARD: HARMFUL.

Product Description
Nucleic acid binding ligand. Binds to A-T-rich regions of the minor groove of DNA.
Product Specific Literature References
Netropsin, a New Antibiotic Produced by a Streptomyces: A.C. Finlay et al.; J. Am. Chem. Soc. 73, 341 (1951)
Efficient Total Syntheses of the Oligopeptide Antibiotics Netropsin and Distamycin: J.W. Lown et al.; J. Org. Chem. 50, 3774 (1985)
Sequence and structural selectivity of nucleic acid binding ligands: J. Ren & J.B. Chaires; Biochemistry 38, 16067 (1999) Abstract
Molecular modelling of the interaction of carbocyclic analogues of netropsin and distamycin with d(CGCGAATTCGCG)2: K. Bielawski, et al.; Acta Biochim. Pol. 47, 855 (2000) Abstract
Recent developments in sequence selective minor groove DNA effectors: B.S. Reddy, et al.; Curr. Med. Chem. 8, 475 (2001), (Review) Abstract
Further Categories Containing This Product:
Other Antibiotics - DNA Regulation / Transcription • Antibiotics - Antiviral / Anti-HIV
 
 
ALX-350-228 Revised 08-Jul-08
Nomilin
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY